応用分子微生物学研究グループ

成果発表

生物システム工学特別研究チーム(旧生物システム工学研究グループ)の成果発表に関しては、こちらをご覧ください。



成果発表(2014年以降)

2018

  • Konishi, K, Ueda S, Kawano M, Osawa S, Tamura T, Hokazono E, Kayamori Y, Sakasegawa S. (2018) Characterization and application of a novel nicotinamide mononucleotide adenylyltransferase from Thermus thermophilus HB8. J Biosci Bioeng. in press.
  • Usuki S, Tamura N, Yuyama K, Tamura T, Mukai K, Igarashi Y. (2018) Konjac ceramide (kCer) regulates NGF-induced neurite outgrowth via the Sema3A signaling pathway. J Oleo Sci 67(1):77-86.
  • Usuki S, Tamura N, Yuyama K, Tamura T, Mukai K, Igarashi Y. (2018) Characterization of konjac ceramide (kCer) binding to Sema3A receptor Nrp1. J Oleo Sci 67(1):87-94.
  • Yasutake Y, Hattori S, Hayashi H, Matsuda K, Tamura N, Kohgo S, Maeda K, Mitsuya H. (2018) HIV-1 with HBV-associated Q151M substitution in RT becomes highly susceptible to entecavir: structural insights into HBV-RT inhibition by entecavir. Sci Rep 8:1624.
  • Itoh H, Miura A, Matsui M, Arazoe T, Nishida K, Kumagai T, Arita M, Tamano K, Machida M, Shibata T. (2018) Knockout of the SREBP system increases production of the polyketide FR901512 in filamentous fungal sp. No. 14919 and lovastatin in Aspergillus terreus ATCC20542. Appl Microbiol Biotechnol 102(3):1393-1405.

2017

  • Kasuga K, Sasaki A, Matsuo T, Yamamoto C, Minato Y, Kuwahara N, Fujii C, Kobayashi M, Agematsu H, Tamura T, Komatsu M, Ishikawa J, Ikeda H, Kojima I. (2017) Heterologous production of kasugamycin, an aminoglycoside antibiotic from Streptomyces kasugaensis, in Streptomyces lividans and Rhodococcus erythropolis L-88 by constitutive expression of the biosynthetic gene cluster. Appl Microbiol Biotechnol 101(10):4259-4268.
  • Yasutake Y, Kameda T, Tamura T. (2017) Structural insights into the mechanism of the drastic changes in enzymatic activity of the cytochrome P450 vitamin D3 hydroxylase (CYP107BR1) caused by a mutation distant from the active site. Acta Crystallogr Sect F 73(5):266-275.
  • Konishi K, Kumagai T, Sakasegawa S, Tamura T. (2017) Complete genome sequence of Burkuhorderia stabilis FERMP-21014. Genome Announcements 5(29):e00636-17.
  • Yasutake Y, Kusada H, Ebuchi T, Hanada S, Kamagata Y, Tamura T, Kimura N. (2017) Bifuncrional quorum-quenching and antibiotic-acylase MacQ forms a 170-kDa capsule-shaped molecule containing spacer polypeptides. Sci Rep 7:8946.
  • Mohd Yusoff MZ, Akita H, Hassan MA, Fujimoto S, Yoshida M, Nakashima N, Hoshino T. (2017) Production of acetoin from hydrothermally pretreated oil mesocarp fiber using metabolically engineered Escherichia coli in a bioreactor system. Bioresour Technol 245(Pt A):1040-1048.
  • Akita H, Kimura ZI, Yusoff MZM, Nakashima N, Hoshino T. (2017) Identification and characterization of Burkholderia multivorans CCA53. BMC Res Notes 10(1):249.
  • Akita H, Nakashima N, Hoshino T. (2017) Production of d-lactate using a pyruvate-producing Escherichia coli strain. Biosci Biotechnol Biochem 81(7):1452-1455.
  • Tamano K, Miura A, Koike H, Kamisaka Y, Umeura M, Machida M. (2017) High-efficiency extracellular release of free fatty acids from Aspergillus oryzae using non-ionic surfactants. J Biotechnol 248:9-14.
  • Matsui M, Yokoyama T, Nemoto K, Kumagai T, Tamano K, Machida M, Shibata T. (2017) Further enhancement of FR901469 productivity by co-overexpression of cpcA, a cross-pathway control gene, and frbF in fungal sp. No. 11243. J Biosci Bioeng 124(1):8-14.
  • Kato S, Kanata Y, Kitagawa W, Sone T, Asano K, Kamagata Y. (2017) Restoration of the growth of Escherichia coli under K+-deficient conditions by Cs+ incorporation via the K+ transporter Kup. Sci Rep 7:1965.
  • Matsui M, Yokoyama T, Nemoto K, Kumagai T, Terai G, Tamano K, Machida M, Shibata T. (2017) Identification of a putative FR901469 biosynthesis gene cluster in fungal sp. No. 11243 and enhancement of the productivity by overexpressing the transcription factor gene frbF. J Biosci Bioeng. 123(2):147-153.

2016

  • Tamano K, Miura A. (2016) Further increased production of free fatty acids by overexpressing a predicted transketolase gene of the pentose phosphate pathway in Aspergillus oryzae faaA disruptant. Biosci Biotechnol Biochem 80(9):1829-1835.
  • Kato S, Goya E, Tanaka M, Kitagawa W, Kikuchi Y, Asano K, Kamagata Y. (2016) Enrichment and isolation of Flavobacterium strains with tolerance to high concentrations of cesium ion. Sci Rep 6:20041.
  • Al-Ashmawy MA, Sallam KI, Abd-Elghany SM, Elhadidy M, Tamura T. (2016) Prevalence, molecular characterization, and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from milk and dairy products. Foodborne Pathog Dis 13(3):156-162.
  • Usuki S, Tamura N, Sakai A, Tamura T, Mukai K, Igarashi Y. (2016) Chemoenzymatically prepared konjac ceramide inhibits NGF-induced neurite outgrowth by a semaphoring 3A-like action. Biochem Biophys Rep 5:160-167.
  • Akita H, Nakashima N, Hoshino T. (2016) Pyruvate production using engineered Escherichia coli. AMB Express 6(1):94.
  • Akita H, Kimura Z, Yusoff MZ, Nakashima N, Hoshino T. (2016) Draft genome sequence of Burkholderia sp. Strain CCA53, isolated from leaf soil. Genome Announc 4(4):e00630-16.
  • Akita H, Kimura Z, Mohd Yusoff MZ, Nakashima N, Hoshino T. (2016) Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential. Springerplus 5:596.
  • 玉野孝一(2016)「代謝物質生産性向上の現在と未来」生物工学会誌 94(9):563.

2015

  • Tamano K, Bruno KS, Koike H, Ishii T, Miura A, Umemura M, Culley DE, Baker SE, Machida M. (2015) Increased production of free fatty acids in Aspergillus oryzae by disruption of a predicted acyl-CoA synthetase gene. Appl Microbiol Biotechnol. 99(7):3103-3113.
  • Matsumoto Y, Yasutake Y, Takeda Y, Tamura T, Yokota A, Wada M. (2015) Structural insights into the substrate stereospecificity of D-threo-3-hydroxyaspartate dehydratase from Delftia sp. HT23: a useful enzyme for the synthesis of optically pure L-threo- and of D-erythro-3-hydroxyaspartate. Appl. Microbiol. Biotechnol, 99(17):7137-7150.
  • Nakamura A, Tamura N, Yasutake Y. (2015) Structure of the HIV-1 reverse transcriptase Q151M mutant: insights into the inhibitor resistance of HIV-1 reverse transcriptase and the structure of the nucleotide-binding pocket of Hepatitis B virus polymerase. Acta Crystallogr Sect F 71(11):1384-1390.
  • Puspita ID, Kitagawa W, Kamagata Y, Tanaka M, Nakatsu CH. (2015) Increase in bacterial colony formation from a permafrost ice wedge dosed with a Tomitella biformata recombinant resuscitation-promoting factor protein. Microbes Environ 30(2):151-156.
  • Ohbayashi T, Takeshita K, Kitagawa W, Nikoh N, Koga R, Meng XY, Tago K, Hori T, Hayatsu M, Asano K, Kamagata Y, Lee BK, Fukatsu T, Kikuchi Y. (2015) Insect’s intestinal organ for symbiont sorting. Proc Natl Acad Sci USA 112(37):E5179-E5188.
  • Mohammed MA. Sallam KI, Tamura T. (2015) Prevalence, identification and molecular characterization of Cronobacter sakazakii isolated from retail meat products. Food Control 53:206-211.
  • Abd-Elghany SM, Sallam KI, Abd-Elkhalek A, Tamura T. (2015) Occurrence, genetic characterization and antimicrobial resistance of Salmonella isolated from chicken meat and giblets. Epidemiol Infect 143(5):997-1003.
  • Sallam KI, Abd-Elghany SM, Elhadidy M, Tamura T. (2015) Molecular characterization and antimicrobial resistance profile of methicillin-resistant Staphylococcus aureus in retail chicken. J Food Prot 78(10):1879-1884.
  • Furukawa J, Sakai S, Yokota I, Okada K, Hamamatsu H, Kobayashi T, Yoshida Y, Higashino K, Tamura T, Igarashi Y, Shinohara Y. (2015) Quantitave GSL-glycome analysis of human whole serum based on an EGCase digestion and glycoblotting method. J Lipid Res 56(12):2399-2407.
  • Akita H, Watanabe M, Suzuki T, Nakashima N, Hoshino T. (2015) Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1. FEMS Microbiol Lett 362(16):fnv116.
  • Akita H, Watanabe M, Suzuki T, Nakashima N, Hoshino T. (2015) Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase. AMB Express 5:17.
  • Akita H, Nakashima N, Hoshino T. (2015) Bacterial production of isobutanol without expensive reagents. Appl Microbiol Biotechnol 99(2):991-999.

2014

  • Tamano K. (2014) Enhancing microbial metabolite and enzyme production: current strategies and challenges. Front Microbiol. 5:718.
  • Takeda I, Tamano K, Yamane N, Ishii T, Miura A, Umemura M, Terai G, Baker SE, Koike H, Machida M. (2014) Genome sequence of the Mucoromycotina fungus Umbelopsis isabellina, an effective producer of lipids. Genome Announc. 2(1):e00071-14.
  • Umemura M, Nagano N, Koike H, Kawano J, Ishii T, Miyamura Y, Kikuchi M, Tamano K, Yu J, Shin-ya K, Machida M. (2014) Characterization of the biosynthetic gene cluster for the ribosomally synthesized cyclic peptide ustiloxin B in Aspergillus flavus. Fungal Genet Biol. 68:23-30.
  • Mohammed MA, Sallam KI, Eldaly EAZ, Ahdy AM, Tamura T. (2014) Occurrence, serotypes and virulence genes of non-O157 Shiga toxin-producing Escherichia coli in fresh beef, ground beef, and beef burger. Food Control 37:182-187.
  • Sallam KI, Mohammed MA, Hassan MA, Tamura T. (2014) Prevalence, molecular identification and antimicrobial resistance profile of Salmonella serovars isolated from retail beef products in Mansoura, Egypt. Food Control 38:209-214.
  • Hubert A, Mitani Y, Tamura T, Boicu M, Nagy I. (2014) Protein complex purification from Thermoplasma acidophilum using a phage display library. J Microbiol Methods 98:15-22.
  • Hayashi, T., Tanaka, Y., Sakai, N., Okada, U., Yao, M., Watanabe, N., Tamura, T, Tanaka, I. (2014) Structural and genomic DNA analysis of the putative TetR transcriptional repressor SCO7518 from Streptomycec coelicoclor A3(2). FEBS Lett 588(23):4311-4318.
  • Kitagawa W, Hata M, Sekizuka T, Kuroda M, Ishikawa J (2014) Draft genome sequence of Rhodococcus erythropolis JCM 6824, an Aurachin RE antibiotic producer. Genome Announc 2(5). pii: e01026-14.
  • Tanaka T, Kawasaki K, Daimon S, Kitagawa W, Yamamoto K, Tamaki H, Tanaka M, Nakatsu CH, Kamagata Y (2014) A hidden pitfall in the preparation of agar media undermines microorganism cultivability. Appl Environ Microbiol 80(24):7659-7666.
  • Enomoto M, Kitagawa W, Yasutake Y, Shimizu H (2014) Total synthesis of aurachins C, D, and L, and a structurally simplified analog of aurachin C. Biosci Biotechnol Biochem 78(8):1324-1327.
  • Yamamoto S, Nakano M, Kitagawa W, Tanaka M, Sone T, Hirai K, Asano K (2014) Characterization of multi-antibiotic-resistant Escherichia coli Isolated from beef cattle in Japan. Microbes Environ 29(2):136-144
  • Kugo T, Kitagawa W, Shimomura Y, Yamagishi T, Tanaka M, Sone T, Asano K, Kamagata Y (2014) Draft genome sequence of methanol-utilizing Methylophilus sp. Strain OH31, isolated from pond sediment in Hokkaido, Japan. Genome Announc 2(2). pii: e00274-14.
  • Funo K, Kitagawa W, Tanaka M, Sone T, Asano K, Kamagata Y (2014) Draft genome sequence of Tomitella biformata AHU 1821T, isolated from a permafrost ice wedge in Alaska. Genome Announc 2(1). pii: e00066-14.
  • Kitagawa W, Kamagata Y (2014) Diversity of 2,4-Dichlorophenoxyacetic Acid (2,4-D)-Degradative Genes and Degrading Bacteria. Biodegradative bacteria-How Bacteria Degrade, Survive, Adapt, and Evolve (Nojiri, H.; Tsuda, M.; Fukuda, M.; Kamagata, Y. Eds.), Chapter 3 (pp 43-57) (Springer Japan), 10.1007/978-4-431-54520-0_3
  • Nakashima N, Ohno S, Yoshikawa K, Shimizu H, Tamura T (2014) A vector library for silencing central carbon-metabolism genes with antisense RNAs in Escherichia coli. Appl Environ Microbiol 80(2):564-573.
  • Yasutake Y, Kitagawa W, Hata M, Nishioka T, Ozaki T, Nishiyama M, Kuzuyama T, Tamura T (2014) Structure of the quinoline N-hydroxylating cytochrome P450 RauA, an essential enzyme that confers antibiotic activity on aurachin alkaloids. FEBS Lett 588(1):105-110.
  • Nakashima N, Akita H, Hoshino T. (2014) Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin. Metab Eng 25:204-214.
  • Nakashima N, Miyazaki K. (2014) Bacterial cellular engineering by genome editing and gene silencing. Int J Mol Sci 15:2773-2793.
  • 安武義晃(2014)「多彩にして多才:シトクロムP450」日本の結晶学 (II) -その輝かしい発展- p.350.(日本結晶学会)
  • 和田大、安武義晃(2014)「反応に金属を要求するPLP酵素」化学と生物 52(10):634-636.

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