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  4. 誌上発表（国際誌・査読有）2015年～2019年

研究成果　Research Result

誌上発表（国際誌・査読有）2019年～2015年

部門研究戦略1：生物機能解明

脳遺伝子研究グループ
Molecular Neurobiology Research Group

  • Ochiishi, T., Kaku, M., Kiyosue, K., Doi, M., Urabe, T., Hattori, N., Shimura, H., Ebihara, T.: New Alzheimer’s disease model mouse specialized for analyzing the function and toxicity of intraneuronal Amyloid β oligomers. Sci Rep, 9(1), 17368 (2019).
  • Ishida, K., Goto, S., Ishimura, M., Amanuma, M., Hara, Y., Suzuki, R., Katoh, K., Morita, E.: Functional correlation between subcellular localizations of Japanese encephalitis virus capsid protein and virus production. J. Virol., 93(19), e00612-19 (2019).
  • Yamamoto, K., Otomo, K., Nemoto, T., Ishihara, S., Haga, H., Nagasaki, A., Murakami, Y., Takahashi, M.: Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts. Exp. Cell Res., 376(1), 67-76 (2019).
  • Tanaka, M., Fujii, Y., Hirano, K., Higaki, T., Nagasaki, A., Ishikawa, R., Okajima, T., Katoh, K.: Fascin in lamellipodia contributes to cell elasticity by controlling the orientation of filamentous actin. 24(3), 202-213 (2019).
  • Saito, R., Shinkai, Y., Doi, M.: Intestinal F-box protein regulates quick avoidance behavior of Caenorhabditis elegans to the pathogenic bacterium Pseudomonas aeruginosa. Genes Cells, (2018).
  • Shinkai, Y., Doi, M.: EOR-1 mediates non-cell autonomous regulation of abts-1 gene expression in HSNs. microPublication Biology (2018).
  • Shinkai, Y., Kuramochi, M., Doi, M.: A simple method for visualization of locus-specific H4K20me1 modifications in living Caenorhabditis elegans single cells. G3-Genes Genomes Genet., 8(7), 2249-2255 (2018).
  • Nagasaki, A., Kato, Y., Meguro, K., Yamagishi, A., Nakamura, C., Uyeda, T.Q.P.: A genome editing vector that enables easy selection and identification of knockout cells. Plasmid, 98, 37-44 (2018).
  • Shinkai, Y., Kuramochi, M., Doi, M.: Regulation of chromatin states and gene expression during HSN neuronal maturation is mediated by EOR-1/PLZF, MAU-2/cohesin loader, and SWI/SNF complex. Sci Rep., 8, 7942 (2018).
  • Kijima, S.T., Staiger, C., Katoh, K., Nagasaki, A., Ito, K., Uyeda, T.Q.: Arabidopsis vegetative actin isoforms, AtACT2 and AtACT7, generate distinct filament arrays in living plant cells. Sci Rep., 8(1) (2018).
  • Ito, N., Katoh, K., Kushige, K., Saito, Y., Umemoto, T., Matsuzaki, Y., Kiyonari, H., Kobayashi, D., Soga, M., Era, T., Araki, N., Furuta, Y., Suda, T., Kida, Y., Ohta, K.: Ribosome incorporation into somatic cells promotes lineage transdifferentiation towards multipotency. Sci. Rep., 8(1), 1634 (2018).
  • Nagasaki, A., Kijima, S.T., Yumoto, T., Imaizumi, M., Yamagishi, A., Kim, H., Nakamura, C., Uyeda, T.Q.P.: The position of the GFP tag on actin affects the filament formation in mammalian cells. 42(2), 131-140 (2017).
  • Nozumi, M., Nakatsu, F., Katoh, K., Igarashi, M.: Coordinated movement of vesicles and actin bundles during nerve growth revealed by superresolution microscopy. Cell Reports, 18, 2203-2216 (2017).
  • Shibata, K., Nagasaki, A., Adachi, H., Uyeda, T.Q.P.: Actin binding domain of filamin distinguishes posterior from anterior actin filaments in migrating Dictyostelium cells. Biophysics and Physicobiology. 13, 321-331 (2016).
  • Ohzono, T., Katoh, K., Fukuda, J.: Fluorescence microscopy reveals molecular localisation at line defects in nematic liquid crystals. Sci. Rep., 6, 36477 (2016).
  • Kien, X.N., Umeki, N., Kijima, S.T., Kodera, N., Ueno, H., Furutani-Umezu, N., Nakajima, J., Noguchi, T.Q.P., Nagasaki, A., Tokuraku, K., Uyeda, T.Q.: Allosteric regulation by cooperative conformational changes of actin laments drives mutually exclusive binding with co lin and myosin. Sci Rep (2016).
  • Hashimoto, M., Doi, M., Kurebayashi, N., Furukawa, K., Hirawake-Mogi, H., Ohmiya, Y., Sakurai, T., Mita, T., Mikoshiba, K., Nara, T.: Inositol 1,4,5-trisphosphate receptor determines intracellular Ca2+ concentration in Trypanosoma cruzi throughout its life cycle. FEBS Open Bio, 6(12), 1178-1185 (2016).
  • Ochiishi, T., Doi, M., Yamasaki, K., Hirose, K., Kitamura, A., Urabe, T., Hattori, N., Kinjo, M., Ebihara, T., Shimura, H.: Development of new fusion proteins for visualizing amyloid-β oligomers in vivo. Sci Rep., 6, 22712 (2016).
  • Ochiishi, T., Itakura, A., Liu, L., Akatsu, H., Kohno, H., Nishimura, M., Yoshimune, K.: Immunohistochemical detection of the delayed formation of nonfibrillar large amyloid-β aggregates. Genes Cells, 21(2), 200-211 (2016).
  • Shibata, T., Yamashita, S., Hirosaki, K., Katoh, K., Ohta, Y.: Isolation of mitochondria by gentle cell membrane 　　disruption, and their subsequent characterization. Biochem. Biophys. Res. Commun., 463(4), 536-568 (2015).

脳機能調節因子研究グループ
Molecular Neurophysiology Research Group

  • Tani, N., Kazuma, K., Ohtsuka, Y., Shigeri, Y., Masuko, K., Konno, K., Inagaki, H.: Mass spectrometry analysis and biological characterization of the predatory ant odontomachus monticola venom and venom sac components. Toxins, 11(1), 1-15 (2019).
  • Murao, N., Matsubara, S., Matsuda, T., Noguchi, H., Mutoh, T., Mutoh, M., Koseki, H., Namihira, M., Nakashima, K: Np95/Uhrf1 regulates tumor suppressor gene expression of neural stem/precursor cells, contributing to neurogenesis in the adult mouse brain. Neurosci. Res. (2018).
  • Semi, K., Sanosaka, T., Namihira, M., Nakashima, K.: Nuclear factor I/A coordinates the timing of oligodendrocyte differentiation/maturation via Olig1 promoter methylation. Hayati Journal of Bioscience, 25(2) (2018).
  • Hirano, K., Namihira, M.: FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1. FEBS Open Bio, 7(12), 1932-1972 (2017).
  • Kazuma, K., Masuko, K., Konno, K., Inagaki, H.: Combined venom gland transcriptomic and venom peptidomic analysis of the predatory ant Odontomachus monticola. Toxins, 9(10), 323 (2017).
  • Noguchi, H., Kimura, A., Murao, N., Namihira, M., Nakashima, K.: Prenatal deletion of DNA methyltransferase 1 in neural stem cells impairs neurogenesis and causes anxiety-like behavior in adulthood. Neurogenesis, 3(1), e1232679 (2016).
  • Hirano, K., Namihira, M.: LSD1 mediates neuronal differentiation of human fetal neural stem cells by controlling the expression of a novel target gene, HEYL. Stem Cells, 34(7), 1872-1882 (2016).
  • Noguchi, H., Murao, N., Kimura, A., Matsuda, T., Namihira, M., Nakashima, K., DNA methyltransferase 1 is indispensable for development of the hippocampal dentate gyrus. J. Neurosci., 36(22), 6050-6068 (2016).
  • Yamauchi, Y., Kimoto, H., Yang, X., Filkin, S., Utkin, Y., Kubo, T., Inagaki, H.: Pr-SNTX, a short-chain three-finger toxin from Papuan pigmy mulga snake, is an antagonist of muscle-type nicotinic acetylcholine receptor (α2βδε). Biosci. Biotechnol. Biochem., 80(1), 158-161 (2016).
  • Noguchi, H., Kimura, A., Murao, N., Matsuda, T., Namihira, M., Nakashima, K.: Expression of DNMT1 in neural stem/precursor cells is critical for survival of newly generated neurons in the adult hippocampus. Neurosci. Res. (2015).
  • Murao, N., Matsuda, T., Noguchi, H., Koseki, H., Namihira, M., Nakashima, K.: Characterization of Np95 expression in mouse brain from embryo to adult: A novel marker for proliferating neural stem/precursor cells. Neurogenesis (2015).
  • Shigeri Y, Yasuda A, Hagihara Y, Nishi K, Watanabe K, Imura T, Inagaki H, Haramoto Y, Ito Y, Asashima M.: Identification of novel peptides from amphibian (Xenopus tropicalis) skin by direct tissue MALDI-MS analysis. FEBS J., 282(1), 102-113 (2015).

細胞分子機能研究グループ
Functional Biomolecular Research Group

  • Sunarso, Tsuchiya, A., Toita, R., Tsuru, K., Ishikawa, K.: Enhanced osseointegration capability of poly(ether ether ketone) via combined phosphate and calcium surface-functionalization. Int. J. Mol. Sci., 21(1), 198 (2019).
  • Toita, R., Asai, D., Otani, K., Kawano, T., Murata, M., Kang, J-H.: Suppression of lysophosphatidylcholine-induced human aortic smooth muscle cell calcification by protein kinase A (PKA) inhibition. Lipids (2019).
  • Asai, D., Murata, M., Toita, R., Kawano, T., Nakashima, H., Kang, JH.: A high-affinity peptide substrate for G protein-coupled receptor kinase 2 (GRK2). Amino Acids (2019).
  • Fukuda, N., Tsuchiya, A., Sunarso, Toita, R., Tsuru, K., Mori, Y., Ishikawa, K.: Surface plasma treatment and phosphorylation enhance the biological performance of poly(ether ether ketone). Colloid Surf. B-Biointerfaces, 173(1), 36-42 (2019).
  • Dwiranti, A., Takata, H., Fukui, K.: Reversible changes of chromosome structure upon different concentrations of divalent cations. Microsc. microanal., 25(3), 817-821 (2019).
  • Saito, E., Isogai, S., Deguchi, T., Ishida, K. Nozaki, T., Ishiama, E., Wayama, M., Shimoda, H.: Intraperitoneal dye injection method for visualizing the functioning lymphatic vascular system in zebrafish and medaka. Dev. Dyn. (2019).
  • Shin, M., Nozaki, T., Idrizi, F. Isogai, S., Ogasawara, K., Ishida, K., Yuge, S., Roscoe, B., Wolfe, S.A., Fukuhara, S., Mochizuki, N., Deguchi, T., Lawson, N.D.: Valves are a conserved feature of the zebrafish lymphatic system. Dev. Cell, 51(3), 374-386 (2019).
  • Fukuda, N., Kanazawa, M., Tsuru, K., Tsuchiya, A., Sunarso, Toita, R., Mori, Y., Nakashima, Y., Ishikawa, K.: Synergistic effect of surface phosphorylation and micro-roughness on enhanced osseointegration ability of poly(ether ether ketone) in the rabbit tibia. Sci Rep, 8(1), 16887 (2018).
  • Toita, R., Otani, K., Kawakno, T., Murata, M., Kang, J-H.: Protein kinase A (PKA) inhibition reduces human aortic smooth muscle cell calcification stimulated by inflammatory response and inorganic phosphate. Life Sci., 209, 466-471 (2018).
  • Toita, R., Fujita, S., Kang, J-H.: Macrophage uptake behavior and anti-inflammatory response of bovine brain- or soybean-derived phosphatidylserine liposomes. J. Oleo Sci., 67(9), 1131-1135 (2018).
  • Sunarso, Tsuchiya, A., Fukuda, N., Toita, R., Tsuru, K., Ishikawa, K.: Effect of micro-roughening of poly(ether ether ketone) on bone marrow derived stem cell and macrophage responses, and osseointegration. J. Biomater. Sci.-Polym. Ed., 1-14 (2018).
  • Toita, R., Kawano, T., Fujita, S., Murata, M., Kang, J-H.: Increased hepatic inflammation in a normal-weight mouse after long-term high-fat diet feeding. J. Toxicol. Pathol., 31(1) (2018).
  • Takata, H., Madung, M., Katoh, K., Fukui, K.: Cdk1-dependent phosphorylation of KIF4A at S1186 triggers lateral chromosome compaction during early mitosis. PLoS One, 13(12), e0209614 (2018).
  • Tonoyama, Y., Shinya, M., Toyoda, A., Kitano, T., Oga, A., Nishimaki, T., Katsumura, T., Oota, H., Wan, M.T., Yip, B.W.P., Helen, M.O.L., Chisada, S., Deguchi, T., Au, D.W.T., Naruse, K., Kamei, Y., Taniguchi, Y.: Abnormal nuclear morphology is independent of longevity in a zmpste24-deficient fish model of Hutchinson-Gilford progeria syndrome (HGPS). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. (2018).
  • Poonperm, R., Takata, H., Uchiyama, S., Fukui, K.: Interdependency and phosphorylation of KIF4 and condensin I are essential for organization of chromosome scaffold. PLoS One, 12(8), e0183298 (2017).
  • Phengchat, R., Takata, H., Uchiyama, S., Fukui, K.: Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore. Sci Rep (2017).
  • Asai, D., Murata, M., Toita, R., Kawano, T., Nakashima, H., Kang, J-H.: Role of amino acid residues surrounding the phosphorylation site in peptide substrates of G protein-coupled receptor kinase 2 (GRK2). Amino Acids, 1-6 (2016).
  • Toita, R., Kawano, T., Murata, M., Kang, J-H.: Anti-obesity and anti-inflammatory effects of macrophage-targeted interleukin-10-conjugated liposome in obese mice. 110(3), 81-88 (2016).
  • Sunarso, Toita, R., Tsuru, K., Ishikawa, K.: Immobilization of calcium and phosphate ions improves the osteoconductivity of titanium implants. Mater. Sci. Eng. C-Mater. Biol. Appl., 68, 291-298 (2016).
  • Sunarso, Toita, R., Tsuru, K., Ishikawa, K.: A superhydrophilic titanium implant functionalized by ozone gas modulates bone marrow cell and macrophage responses. J. Mater. Sci., 27(8), 127-135 (2016).
  • Ohnishi, H., Kawasaki, T., Deguchi, T., Yuba, S.: Generation of xeroderma pigmentosum-A patient-derived induced pluripotent stem cell line for use as future disease model. Cell. Reprogramm., 17(4), 268-274 (2015).

部門研究戦略2：生物機能計測

バイオアナリティカル研究グループ
Bioanalytical Research Group

  • Chihara, K., Bischler, T., Barquist, L., Monzon, V.A., Noda, N., Vogel, J., Tsuneda, S.: Conditional Hfq association with small noncoding RNAs in Pseudomonas aeruginosa revealed through comparative UV cross-linking immunoprecipitation followed by high-throughput sequencing. mSystems (2019).
  • Ishikawa, D., Suzuki, Y., Kurokawa, C., Ohara, M., Tsuchiya, M., Morita, M., Yanagisawa, M., Endo, M., Kawano, R., Takinoue, M.: DNA Origami nanoplate‐based emulsion with nanopore function. Angew. Chem.-Int. Edit., 58, 15299-15303 (2019).
  • Hayakawa, E., Ohgidani, M., Fujimura, Y., Kanba, S., Miura, D., Kato, T.A.: Cuprizone-treated mice, a possible model of schizophrenia, highlighting the simultaneous abnormalities of GABA, serine and glycine in hippocampus. Schizophr. Res., 210, 326-328 (2019).
  • Morikawa-Ichinose, T., Fujimura, Y., Murayama, F., Yamazaki, Y., Yamamoto, T., Wariishi, H., Miura, D.: Improvement of sensitivity and reproducibility for imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry. J. Am. Soc. Mass Spectrom., 30(8), 1512-1520 (2019).
  • Sasaki, A., Halter, M., Elliott, J.T.: Fluorescence correlation methods for determining absolute numbers of molecules from microscopy images. Bioimages, 27, 13-22 (2019).
  • Ota, Y., Saito, K., Takagi, T., Matsukura, S., Morita, M., Tsuneda, S., Noda, N.: Fluorescent nucleic acid probe in droplets for bacterial sorting (FNAP-sort) as a high-throughput screening method for environmental bacteria with various growth rates. PLoS One, 14(4), e0214533 (2019).
  • Watahiki, S., Kimura, N., Yamazoe, A., Miura, T., Sekiguchi, Y., Noda, N., Matsukura, S., Kasai, D., Takahata, Y., Nojiri, H., Fukuda, M.: Ecological impact assessment of a bioaugmentation site on remediation of chlorinated ethylenes by multi-omics analysis. J. Gen. Appl. Microbiol. (2019).
  • Morita, M., Ota, Y., Katoh, K., Noda, N.: Bacterial cell culture at the single-cell level inside giant vesicles. J. Vis. Exp. (2019).
  • Morita, M., Katoh, K., Noda, N.: Direct observation of bacterial growth in giant unilamellar vesicles: A novel tool for bacterial culture. ChemistryOpen, 7(11), 845-849 (2018).
  • Miyamoto, T., Yokota, A., Ota, Y., Tsuruga, M., Aoi, R., Tsuneda, S., Noda, N.: Nitrosomonas europaea MazF specifically recognises the UGG motif and promotes selective RNA degradation. Front. Microbiol.9, 2386 (2018).
  • Sasaki, A., Yamamoto, J., Kinjo, M., Noda, N.: Absolute quantification of RNA molecules using fluorescence correlation spectroscopy with certified reference materials. Anal. Chem., 90(18), 10865-10871 (2018).
  • Tourlousse, D.M., Ohashi, A., Sekiguchi, Y.: Sample tracking in microbiome community profiling assays using synthetic 16S rRNA gene spike-in controls. Sci Rep, 8(1), 9095 (2018).
  • Sato, Y., Endo, M., Morita, M., Takinoue, M., Sugiyama, H., Murata, S., Nomura, S.M., Suzuki, Y.: Environment-dependent self-assembly of DNA origami lattices on phase-separated lipid membranes. Adv. Mater. Interfaces, 5(14), 1-6 (2018).
  • Sugawara, J., Tomota, T., Noda, N., Matsukura, S., Tsukagoshi, K., Hayashi, K., Hieda, M., Maeda, S.: Effects of endothelin-related gene polymorphisms and aerobic exercise habit on age-related arterial stiffening: a 10-yr longitudinal study. J. Appl. Physiol., 124, 312-320 (2018).
  • Itoh, N., Takada, E., Okubo, K., Yano, Y., Hoshino, M., Sasaki, A., Kinjo, M., Matsuzaki, K.: Not oligomers but amyloids are cytotoxic in the membrane-mediated amyloidogenesis of amyloid-β peptides. ChemBioChem (2017).
  • Tsuboi, S., Sasaki, A., Sakata, T., Yasuda, H., Jin, T.: Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging. Chem. Commun., 53(68), 9450-9453 (2017).
  • Miyamoto, T., Ota, Y., Yokota, A., Suyama, T., Tsuneda, S., Noda, N.: Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease. MicrobiologyOpen, e501 (2017).
  • Kurokawa, C., Fujiwara, K., Morita, M., Kawamata, I., Kawagishi, Y., Sakai, A., Murayama, Y., Nomura, S.M., Murata, S., Takinoue, M., Yanagisawa, M.: DNA cytoskeleton for stabilizing artificial cells. Proc. Natl. Acad. Sci. U. S. A., 114(28), 7228-7233 (2017).
  • Hermawan, I., Furuta, A., Higashi, M., Fujita, Y., Akimitsu, N., Yamashita, A., Moriishi, K., Tsuneda, S., Tani, H., Nakakoshi, M., Tsubuki, M., Sekiguchi, Y., Noda, N., Tanaka, J.: Four aromatic sulfates with an inhibitory effect against HCV NS3 helicase from the crinoid alloeocomatella polycladia. Mar. Drugs, 15(4), 117 (2017).
  • Tourlousee DM., Yoshiike, S., Ohashi, A., Matsukura, S., Noda, N., Sekiguchi, Y.: Synthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing. Nucleic Acids Res., 45(4), e23 (2017).
  • Miyamoto, T., Yokota, A., Tsuneda, S., Noda, N.: AAU-specific RNA cleavage mediated by MazF toxin endoribonuclease conserved in Nitrosomonas europaea. Toxins, 8(6), 174 (2016).
  • Morikawa, T.J., Fujita, H., Kitamura, A., Horio, T., Yamamoto, J., Kinjo, M., Sasaki, A., Machiyama, H., Yoshizawa, K., Ichimura, T., Imada, K., Nagai, T., Watanabe, T.M.: Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Sci Rep, 6, 22342 (2016).
  • Goto, K., Kimura, T., Kitamura, N., Semba, S., Ohmiya, Y., Aburatani, S., Matsukura, S., Tsuda, M., Kurokawa, T., Gong, J.P., Tnaka, S., Yasuda, K.: Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 Cells, which plays a significant role in the gel-induced chondrogenic differentiation. J. Biomed. Mater. Res. Part A, 104(3), 734-746 (2016).
  • Miyamoto, T., Kato, Y., Sekiguchi, Y., Tsuneda, S., Noda, N.: Characterization of mazF-mediated sequence-specific RNA cleavage in pseudomonas putida using massive parallel sequencing. PLoS One, 11(2), e0149494 (2016).
  • Fujimoto, N., Mizuno, K., Yokoyama, T., Ohnishi, A., Suzuki, M., Watanabe, S., Komatsu, K., Sakata, Y., Kishida, N., Akiba, M., Matsukura, S.: Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing. J. Gen. Appl. Microbiol., 61(5), 171-176 (2015).
  • Tourlousse DM., Natsuura, N., Sun, L., Toyonaga, M., Kuroda, K., Ohashi, A., Cruz, R., Yamaguchi, T., Sekiguchi, Y.: Draft genome sequence of bacteroidales strain TBC1, a novel isolate from a methanogenic wastewater treatment system. Genome Announc., 3(5), e01168 (2015).
  • Tourlousse DM., Honda, T., Matsuura, N., Ohashi, A., Tonouchi, A., Sekiguchi, Y.: Draft genome sequence of bacteroidales strain 6E, isolated from a rice paddy field in Japan. Genome Announc., 3(5), e01167 (2015).
  • Sasaki, A., Yamamoto, J., Takashi, J., Kinjo, M.: Raster image cross-correlation analysis for spatiotemporal visualization of intracellular degradation activities against exogenous DNAs. Sci Rep., 5, 14428 (2015).
  • Oasa, S., Sasaki, A., Yamamoto, J., Mikuni, S., Kinjo, M.: Homodimerization of glucocorticoid receptor from single cells investigated using fluorescence correlation spectroscopy and microwells. FEBS Lett., 589(17), 2171-2178 (2015).
  • Furuta, A., Tsubuki, M., Endoh, M., Miyamoto, T., Tanaka, J., Salam, K.A., Akimitsu, N., Tani, H., Yamashita, A., Moriishi, K., Nakakoshi, M., Sekiguchi, Y., Tsuneda, S., Noda, N.: Identification of hydroxyanthraquinones as novel inhibitors of hepatitis C virus NS3 helicase. Int. J. Mol. Sci., 16, 18439-18453 (2015).
  • Mikuni, S., Kodama, K., Sasaki, A., Kohira, N., Maki, H., Munetomo, M., Maenaka, K., Kinjo, M.: Screening for FtsZ dimerization inhibitors using fluorescence cross-correlation spectroscopy and surface resonance plasmon analysis. PLoS One, 10(7), e0130933 (2015).
  • Sasaki, A., Tsukasaki, Y., Komatsuzaki, A., Sakata, T., Yasuda, H., Jin, T.: Recombinant protein (EGFP-Protein G) coated PbS quantum dots for in vitro and in vivo dual fluorescence (visible and second-NIR) imaging of breast tumors. Nanoscale, 7(12), 5115-5119 (2015).
  • Shirane, S., Araki, M., Morishita, S., Edahiro, Y., Takei, H., Yoo, Y., Choi, M., Sunami, Y., Hironaka, Y., Noguchi, M., Koike, M., Noda, N., Ohsaka, A., Komatsu, N.: JAK2, CALR and MPL mutation spectrum in Japanese myeloproliferative neoplasms patients. Haematologica, 100(2), e46-48 (2015).

細胞・生体医工学研究グループ
Medical and Biological Engineering Research Group

  • Kurihara, K., Homma, T., Kobayashi, S., Shichiri, M., Fujiwara, H., Fujii, S., Yamada, KI., Nakane, M., Kawamae, K., Fujii, J.: Ascorbic acid insufficiency impairs spatial memory formation in juvenile AKR1A-knockout mice. J. Clin. Biochem. Nutr., 65(3), 209-216 (2019).
  • Yamazoe, H.: Antibody immobilization technique using protein film for high stability and orientation control of the immobilized antibody. Mater. Sci. Eng. C-Mater. Biol. Appl., 100, 209-214(2019).
  • Shichiri, M., Ishida, N., Hagihara, Y., Yoshida, Y., Kume, A., Suzuki, H.: Probucol induces the generation of lipid peroxidation products in erythrocytes and plasma of male cynomolgus macaques. J. Clin. Biochem. Nutr., 64(2), 129-142 (2019).
  • Nautiyal, K., Kibria, M.G., Akazawa-Ogawa, Y., Hagihara, Y., Kuroda, Y.: Design and assessment of an active anti-epidermal growth factor receptor (EGFR) single chain variable fragment (ScFv) with improved solubility. Biochem. Biophys. Res. Commun., 508(4), 1043-1049 (2018).
  • Suzuki, T., Mochizuki, Y., Kimura, S., Akazawa-Ogawa, Y., Hagihara, Y., Nemoto, N.: Anti-survivin single-domain antibodies derived from an artificial library including three synthetic random regions by in vitro selection using cDNA display. Biochem. Biophys. Res. Commun., 503(3), 2054-2060 (2018).
  • Soeta, Y., Sakamoto, Y.: An exploratory analysis of sound field characteristics using the impulse response in a car cabin. Environments, 5(4), 44 (2018).
  • Yamazoe, H.: Spectroscopic study on the conformation of serum albumin in film state. J. Biosci. Bioeng. (2018).
  • Furutani, S., Nishio, K., Naruishi, N., Akazawa-Ogawa, Y., Hagihara, Y., Yoshida, Y., Nagai, H.: Rapid enzyme-linked immunosorbent assays for diagnosis of diabetes in a compact disc-shaped microfluidic device. Anal. Sci., 34(3), 379-382 (2018).
  • Soeta, Y., Shimokura, R.: Sound quality evaluation of air-conditioner noise based on factors of the autocorrelation function. Appl. Acoust., 124, 11-19 (2017).
  • Dong, JH., Shichiri, M., Chung, CI., Shibata, T., Uchida, K., Hagihara, Y., Yoshida, Y., Ueda, H.: An open sandwich immunoassay for detection of 13(R,S)-hydroxy-9(E),11(E)-octadecadienoic acid. Analyst, 142(5), 787-793 (2017).
  • Homma, T., Akihara, R., Okano, S., Shichiri, M., Yoshida, Y., Yamada, K., Miyata, S., Nakajima, O., Fujii, J.: Heightened aggressive behavior in mice deficient in aldo-keto reductase 1a (Akr1a). Behav. Brain Res., 319, 219-224 (2017).
  • Yamazoe, H.: Multifunctional protein microparticles for medical applications. Biomaterials, 155, 1-12 (2017).
  • Furutani, S., Hagihara, Y., Nagai, H.: On-site identification of meat species in processed foods by a rapid real-time polymerase chain reaction system. Meat Sci., 131, 56-59 (2017).
  • Kume, A., Anh, DTM., Shichiri, M., Ishida, N., Suzuki, H.: Probucol dramatically enhances dihydroartemisinin effect in murine malaria. Malar. J., 15, 472 (2016).
  • Otsuki, N., Homma, T., Fujiwara, H., Kaneko, K., Hozumi, Y., Shichiri, M., Takashima, M., Ito, J., Konno, T., Kurahashi, T., Yoshida, Y., Goto, K., Fujii, S., Fujii, J.: Trichloroethylene exposure aggravates behavioral abnormalities in mice that are deficient in superoxide dismutase. Regul. Toxicol. Pharmacol., 79, 83-90 (2016).
  • Saito, Y., Akazawa-Ogawa, Y., Matsumura, A., Saigoh, K., Itoh, S., Sutou, K., Kobayashi, M., Mita, Y., Shichiri, M., Hisahara, S., Hara, Y., Fujimura, H., Takamatsu, H., Hagihara, Y., Yoshida, Y., Hamakubo, T., Kusunoki, S., Shimohama, S., Noguchi, N.: Oxidation and interaction of DJ-1 with 20S proteasome in the erythrocytes of early stage Parkinson’s disease patients. Sci Rep., 6, 30793 (2016).
  • Noda, S., So, M., Adachi, M., Kardos, J., Akazawa-Ogawa, Y., Hagihara, Y., Goto, Y.: Thioflavin T-silent denaturation intermediates support the main-chain-dominated architecture of amyloid fibrils. Biochemistry, 55(28), 3937-3948 (2016).
  • Soeta, Y., Nakagawa, S., Kamiya, Y., Kamiya, M.: Subjective preference for air-conditioner sounds inside a car in summer and winter. J. Ergonomics, 6(4) (2016).
  • Akazawa-Ogawa, Y., Uegaki, K., Hagihara, Y.: The role of intra-domain disulfide bonds in heat-induced irreversible denaturation of camelid single domain VHH antibodies. J. Biochem., 159(1), 111-121 (2016).
  • Kume, A., Herbas, M.S., Shichiri, M., Ishida, N., Suzuki, H.: Effect of anti-hyperlipidemia drugs on the alpha-tocopherol concentration and their potential for murine malaria infection. Parasitol. Res., 115(1), 69-75 (2016).
  • Ando, M., Horie, M., Akazawa-Ogawa, Y., Hagihara, Y., Murase, N., Shigeri, Y.: Cytotoxicity of CdSe-based quantum dots incorporated in glass nanoparticles evaluated using human keratinocyte HaCaT cells. Biosci. Biotechnol. Biochem., 80(1), 210-213 (2016).
  • Yamazoe, H., Nakanishi, H., Kashiwagi, Y., Nakamoto, M., Tachibana, A., Hagihara, Y., Tanabe, T.: Changes in cell adhesiveness and physicochemical properties of crosslinked albumin films after ultraviolet irradiation. Langmuir, 32(1), 203-210 (2016).
  • Yamazoe, H., Ichikawa, T., Hagihara, Y., Iwasaki, Y.: Generation of a patterned co-culture system composed of adherent cells and immobilized nonadherent cells. Acta Biomater., 31, 231-240 (2016).
  • Yamazoe, H., Hagihara, Y., Kobayashi, H.: Multi-component co-culture system of cancer cells and two types of stromal cells for in vitro evaluation of anticancer drugs. Tissue Eng., 22(1), 20-29 (2016).
  • Soeta, Y., Nakagawa, S.: Prediction of optimal auditory signals using auditory evoked magnetic responses. Build. Environ. , 94, 924-929 (2015).
  • Saito, Y., Shichiri, M., Hamajima, T., Ishida, N., Mita, Y., Nakao, S., Hagihara, Y., Yoshida, Y., Takahashi, K., Niki, E., Noguchi, N.: Enhancement of lipid peroxidation and its amelioration by vitamin E in a subject with mutations in the SBP2 gene. J. Lipid Res., 56(11), 2172-2182 (2015).
  • Herbas, M.S., Shichiri, M., Ishida, N., Kume, A., Hagihara, Y., Yoshida, Y., Suzuki, H.: Probucol-induced α-tocopherol deficiency protects mice against malaria infection. PLoS One, 10(8), e0136014 (2015).
  • Miyazaki, N., Kiyose, N., Akazawa, Y., Takashima, M., Hagihara, Y., Inoue, N., Matsuda, T., Ogawa, R., Inoue, S., Ito, Y.: Isolation and characterization of antigen-specific alpaca (Lama pacos) VHH antibodies by biopanning followed by high throughput sequencing. J. Biochem., 158(3), 205-215 (2015).
  • Soeta, Y., Shimokura, R., Kim, Y.H., Ohsawa, T., Ito, K.: Characteristics of Buddhist chanting in Japanese temples. Appl. Acoust., 91, 73-76 (2015).
  • Soeta, Y., Bito, Y.: Detection of features of prosthetic cardiac valve sound by spectrogram analysis. Appl. Acoust., 89, 28-33 (2015).
  • Yoshino, K., Umeno, A., Shichiri, M., Watanabe, H., Ishida, N., Kojima, M., Iwaki, S., Hagihara, Y., Nakamura, M., Yoshida, Y.: Biomarkers for the evaluation of immunological properties during the Shikoku walking pilgrimage. J. Biol. Regul. Homeost. Agents, 29(1), 51-62 (2015).
  • Yagi, H., Mizuno, A., So, M., Hirano, M., Adachi, M., Akazawa-Ogawa, Y., Hagihara, Y., Ikenoue, T., Lee, Y-H., Kawata, Y., Goto, Y.: Ultrasonication-dependent formation and degradation of α-synuclein amyloid fibrils. BBA-Proteins Proteomics, 1854(3), 209-217 (2015).
  • Akazawa-Ogawa, Y., Shichiri, M., Nishio, K., Yoshida, Y., Niki, E., Hagihara, Y.: Singlet oxygen-derived products from linoleate activate Nrf2 signaling in skin cells. Free Radic. Biol. Med., 79, 164-175 (2015).
  • Ando, M., Horie, M., Akazawa-Ogawa, Y., Hagihara, Y., Murase, N., Shigeri, Y.: Cytotoxicity of CdSe-based quantum dots incorporated in glass nanoparticles evaluated using human keratinocyte HaCaT cells. Biosci. Biotechnol. Biochem., 80(1), 210-213 (2015).
  • Yoshino, K., Umeno, A., Shichiri, M., Watanabe, H., Ishida, N., Kojima, M., Iwaki, S., Hagihara, Y., Nakamura, M., Yoshida, Y.: Biomarkers for the evaluation of immunological properties during the shikoku walking pilgrimage. J. Biol. Regul. Homeost. Agents, 29(1), 51-62 (2015).

部門研究戦略3：生物機能応用

分子細胞デザイン研究グループ
Molecular and Cellular Design Research Group

  • Shibakami, M., Sohma, M., Kijima, N., Nemoto, T.: Melt spinnabilities of thermoplastic paramylon mixed esters. Heliyon, 5(11), e02843 (2019).
  • Watanabe, H., Yoshida, C., Ooishi, A., Nakai, Y., Ueda, M., Isobe, Y., Honda, S.: Histidine-mediated intramolecular electrostatic repulsion for controlling pH-dependent protein-protein interaction. ACS Chem. Biol., 14(12), 2729-2736 (2019).
  • Nasrin, S.R., Kabir, A.M.R., Konagaya, A., Ishihara, T., Sada, K., Kakugo, A.: Stabilization of microtubules by cevipabulin. Biochem. Biophys. Res. Commun., 516(3), 760-764 (2019).
  • Akizuki, K., Kinumi, T., Ono, A., Senga, Y., Osawa, J., Shigeri, Y., Ishida, A., Kameshita, I., Sueyoshi, N.: Autoactivation of C-terminally truncated Ca2+/calmodulin-dependent protein kinase (CaMK) Iδ via CaMK kinase-independent autophosphorylation. Arch. Biochem. Biophys., 15(668), 29-38 (2019).
  • Senga, Y., Imamura, H., Ogura, T., Honda, S.: In-solution microscopic imaging of fractal aggregates of a stressed therapeutic antibody. Anal. Chem. (2019).
  • Shibakami, M., Shibata, K., Akashi, A., Onaka, N., Takezaki, J., Tsubouchi, G., Yoshikawa, H.: Creation of straight-chain cationic polysaccharide-based bile salt sequestrants made from euglenoid β-1,3-glucan as potential antidiabetic agents. Pharm. Res., 36(1), 23 (2019).
  • Shibakami, M., Sohma, M.: Thermal, crystalline, and pressure-sensitive adhesive properties of paramylon monoesters derived from an euglenoid polysaccharide. Carbohydr. Polym. 200, 239-247 (2018).
  • Senga, Y., Honda, S.: Suppression of aggregation of therapeutic monoclonal antibodies during storage by removal of aggregation precursors using a specific adsorbent of non-native IgG conformers. Bioconjugate Chem., 29(10), 3250-3261 (2018).
  • Yamashita, M., Sueyoshi, N., Yamada, H., Katayama, S., Senga, Y., Takenaka, Y., Ishida, A., Kameshita, I., Shigeri, Y.: Characterization of CoPK02, a Ca2+/calmodulin-dependent protein kinase in mushroom Coprinopsis cinerea. Biosci. Biotechnol. Biochem., 82(8), 1335-1343 (2018).
  • Shibakami, M., Nemoto, T., Sohma, M.: Dependence of dissolution, dispersion, and aggregation characteristics of cationic polysaccharides made from euglenoid β-1,3-glucan on degree of substitution. Cellulose, 25(4), 2217-2234 (2018).
  • Shibakami, M.: Thickening and water-absorbing agent made from euglenoid polysaccharide. Carbohydr. Polym., 173, 451-464 (2017).
  • Senga, Y., Imamura, H., Miyafusa, T., Watanabe, H., Honda, S.: AlphaScreen-based homogeneous assay using a pair of 25-residue artificial proteins for high-throughput analysis of non-native IgG. Sci Rep., 7(1), 12466 (2017).
  • Shibakami, M., Sohma, M.: Synthesis and thermal properties of paramylon mixed esters and optical, mechanical, and crystal properties of their hot-pressed films. Carbohydr. Polym., 155, 416-424 (2017).
  • Sugiyama, Y., Yamashita, S., Uezato, Y., Senga, Y., Katayama, S., Goshima, N., Shigeri, Y., Sueyoshi, N., Kameshita, I.: Phosphorylated TandeMBP: A unique protein substrate for protein phosphatase assay. Anal. Biochem., 513, 47-53 (2016).
  • Watanabe, H., Yageta, S., Imamura, H., Honda, S.: Biosensing probe for quality control monitoring of the structural integrity of therapeutic antibodies. Anal. Chem., 88(20), 10095-10101 (2016).
  • Shibakami, M., Tsubouchi, G., Sohma, M., Hayashi, M.: Synthesis of nanofiber-formable carboxymethylated Euglena-derived β-1,3-glucan. Carbohydr. Polym. 152(18), 468-478 (2016).
  • Senga, Y., Akizuki, K., Katayama, S., Shigeri, Y., Kameshita, I., Ishida, A., Sueyoshi, N.: High-performance CaMKI: A highly active and stable form of CaMKIδ produced by high-level soluble expression in Escherichia coli. Biochem. Biophys. Res. Commun., 475(3), 277-282 (2016).
  • Katayama, S., Senga, Y., Oi, A., Miki, Y., Sugiyama, Y., Sueyoshi, N., Kameshita, I.: Expression analyses of splice variants of zebrafish cyclin-dependent kinase-like 5 and its substrate, amphiphysin 1. Gene, 583(1), 15-23 (2016).
  • Shibakami, M., Tsubouchi, G., Sohma, M., Hayashi, M.: Preparation of self-standing transparent thin films made from acetylated euglenoid β-1,3-glucans. Carbohydr. Polym., 133, 421-428 (2015).
  • Watanabe, H., Honda, S.: Adaptive assembly: maximizing the potential of a given functional peptide with a tailor-made protein scaffold. Chem. Biol., 22(9) (2015).
  • Senga, Y., Ishida, A., Shigeri, Y., Kameshita, I., Sueyoshi, N.: The phosphatase-resistant isoform of CaMKI, Ca²⁺/calmodulin-dependent protein kinase Iδ (CaMKIδ), remains in its "primed" form without Ca²⁺ stimulation. Biochemistry, 54(23), 3617-3630 (2015).
  • Shibakami, M., Tsubouchi, G., Sohma, M., Hayashi, M.: One-pot synthesis of thermoplastic mixed paramylon esters using trifuoroacetic anhydride. Carbohydr. Polym., 119, 1-7 (2015).

構造創薬研究グループ
Structure-Based Drug Discovery Research Group

  • Akutsu, Y., Doi, M., Furukawa, K., Takagi, Y.: Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System. J. Vis. Exp., 149, e59962 (2019).
  • Yamasaki, K., Kubota, T., Yamasaki, T., Nagashima, I., Shimizu, H., Terada, RI, Nishigami, H., Kang, J., Tateno, M., Tateno, H.: Structural basis for specific recognition of core fucosylation in N-glycans by Pholiota squarrosa lectin (PhoSL). GLYCOBIOLOGY, 29(7), 576-587 (2019).
  • Nishigami, H., Kang, J., Terada, R., Kino, H., Yamasaki, K., Tateno, M.: Is it possible for short peptide composed of positively- and negatively-charged “hydrophilic” amino acid residue-clusters to form metastable “hydrophobic” packing?. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 21(19), 9683-9693 (2019).
  • Phillips, R.E., Yang, Y., Smith, R.C., Thompson, B.M., Yamasaki, T., Sato-Feliciano, Y.M., Funato, K., Liang, Y., Garcia-Bermudez, J., Wang, X., Garcia, B.A., Yamasaki, K., McDonald, J.G., Birsoy, K., Tabar, V., Allis, C.D.: Target identification reveals lanosterol synthase as a vulnerability in glioma. Proc. Natl. Acad. Sci. U. S. A., 116(16), 7957-7962 (2019).
  • Nishigami, H., Kang, J., Yamasaki, K., Tateno, M.: Specific coordination mode and electrostatic Ion atmosphere effects of divalent cations regulating spatially crossing configurations of DNA duplexes. J. Phys. Soc. Jpn., 88, 44801 (2019).
  • Furuhata, Y., Kobayashi, M., Maruyama, R., Sato, Y., Makino, K., Michiue, T., Yui, H., Nishizawa, S., Yoshimoto, K.: Programmable RNA detection with a fluorescent RNA aptamer using optimized three-way junction formation. RNA (2019).
  • Furuhata, Y., Sakai, A., Murakami, T., Morikawa, M., Nakamura, C., Yoshizumi, T., Fujikura, U., Nishida, K., Kato, Y.: A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall. Sci Rep, 9, 2163 (2019).
  • Takagi, Y., Akutsu, Y., Doi, M., Furukawa, K.: Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi. Plos Neglect. Trop. Dis. (2019).
  • Takagi, Y., Nosato, H., Doi, M., Furukawa, K., Sakanashi, H.: Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach. Biotechniques, 66(3) (2018).
  • Tani, O., Akutsu, Y., Ito, S., Suzuki, T., Tateishi, Y., Yamaguchi, T., Niimi, T., Namatame, I., Chiba, Y., Sakashita, H., Kubota, T., Yanagi, T., Mizukami, S., Hirayama, K., Furukawa, K., Yamasaki, K.: NMR biochemical assay for oxidosqualene cyclase: Evaluation of inhibitor activities on trypanosoma cruzi and human enzymes. J. Med. Chem., 61, 5047-5053 (2018).
  • Yamasaki, K., Yamasaki, T., Tateno, H.: The trimeric solution structure and fucose-binding mechanism of the core fucosylation-specific lectin PhoSL. Sci Rep., 8(7740), 1-12 (2018).
  • Ishitobi, H., Sanada, Y., Kato, Y., Ikuta, Y., Shibata, S., Yamasaki, S., Lotz, M.K., Matsubara, K., Miyaki, S., Adachi, N.: Carnosic acid attenuates cartilage degeneration through induction of heme oxygenase-1 in human articular chondrocytes. Eur. J. Pharmacol., 830, 1-8 (2018).
  • Kubota, T., Tani, O., Yamaguchi, T., Namatame, I., Sakashita, H., Furukawa, K., Yamasaki, K.: Crystal structures of FMN-bound and FMN-free forms of dihydroorotate dehydrogenase from Trypanosoma brucei. FEBS Open Bio (2018).
  • Yamashita, S., Takagi, Y., Nagaike, T., Tomita, K.: Crystal structures of U6 snRNA-specific terminal uridylyltransferase. Nat. Commun., 8, 15788 (2017).
  • Kang, J., Yamasaki, K., Sano, K., Tsutsui, K., Tsutsui, K.M., Tateno, M.: Simulated annealing-extended sampling for multicomponent decomposition of spectral data of DNA complexed with peptide. J. Phys. Soc. Jpn. 86, 014802 (2016).
  • Furuta, T., Miyake, S., Ishitobi, H., Ogura, T., Kato, Y., Kamei, N., Miyado, K., Higashi, Y., Ochi, M.: Mesenchymal stem cell-derived exosomes promote fracture healing in a mouse model. Stem Cells Transl. Med. 5(12), 1620-1630 (2016).
  • Yamasaki, K., Tani, O., Tateishi, Y., Tanabe, E., Namatame, I., Niimi, T., Furukawa, K., Sakashita, H.: An NMR biochemical assay for fragment-based drug discovery: evaluation of an inhibitor activity on spermidine synthase of Trypanosoma cruzi. J. Med. Chem., 59(5), 2261-2266 (2016).

生体分子創製研究グループ
Biomolecule Design Research Group

  • Yamano, N., Kawasaki, N., Ida, S., Nakayama, A.: Biodegradation of polyamide 4 in seawater. Polym. Degrad. Stabil., 166, 230-236 (2019).
  • Himiyama, T., Oshima, M., Uegaki, K., Nakamura, T.: Distinct molecular assembly of homologous peroxiredoxins from Pyrococcus horikoshii and Thermococcus kodakaraensis. J. Biochem., 106(1), 89-95 (2019).
  • Masui, A., Ikawa, S., Kawasaki, N., Yamano, N., Nakayama, A.: Biodegradation control of a polyamide 4–visible-light-sensitive TiO2 composite by a fluorescent light irradiation. Polym. Degrad. Stabil., 167, 44-49 (2019).
  • Nakayama, A., Yamano, N., Kawasaki, N.: Biodegradation in seawater of aliphatic polyesters. Polym. Degrad. Stabil., 166, 290-299 (2019).
  • Mine, S., Watanabe, M.: Structural insights into the molecular evolution of the Archaeal Exo-β-d-Glucosaminidase. Int. J. Mol. Sci., 20(10), 2460 (2019).
  • Nakamura, T., Koma, D., Oshima, M., Hoshino, H., Ohmoto, T., Uegaki, K.: Application of chromosomal gene insertion into Escherichia coli for expression of recombinant proteins. J. Biosci. Bioeng., 126(2), 266-272 (2018).
  • Sashiwa, H., Fukuda, R., Okura, T., Sato, S., Nakayama, A.: Microbial degradation behavior in seawater of polyester blends containing poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Mar. Drugs, 16(34) (2018).
  • Mine, S., Watanabe, M., Kamachi, S., Abe, Y., Ueda, T.: The structure of an archaeal ß-gulucosaminidase provides insight into glycoside hydrolase evolution. J. Biol. Chem., 292, 4996-5006 (2017).
  • Yamano, N., Kawasaki, N., Ida, S., Nakayama, Y., Nakayama, A.: Biodegradation of polyamide 4 in vivo. Polym. Degrad. Stabil., 137, 281-288 (2017).
  • Uechi, K., Kamachi, S., Akita, H., Mine, S., Watanabe, M.: Crystal structure of an acetyl esterase complexed with acetate ion provides insight into the catalytic mechanism. Biochem. Biophys. Res. Commun., 477(3), 383-387 (2016).
  • Kawasaki, T., Saka, T., Mine, S., Mizohata, E., Inoue, T., Matsumura, H., Sato, T.: N-terminal acidic residue of the cytosolic helix 8 of an odorant receptor is responsible for different response dynamics via G-protein. FEBS Lett., 589(10), 1136-1142 (2015).
  • Kawasaki, N., Yamano, N., Nakayama, A.: Polyamide4-block-poly(vinyl acetate) via a polyamide4 azo macromolecular initiator: Thermal and mechanical behavior, biodegradation, and morphology. J. Appl. Polym. Sci., 132(37), 42466-42475 (2015).
  • Ando, H., Kawasaki, N., Yamano, N., Uegaki, K., Nakayama, A.: Biodegradation of a poly(ε-caprolactone-co-L-lactide)–visible-light-sensitive TiO2 composite with an on/off biodegradation function. Polym. Degrad. Stabil., 114, 65-71 (2015).

先端ゲノムデザイン研究グループ
Advance Genome Design Research Group

  • Hao, L., Okano, K., Zhang, C., Zhang, Z., Lei, Z., Feng, C., Utsumi, M., Ihara, I., Maseda, H., Shimizu, K.: Effects of levofloxacin exposure on sequencing batch reactor (SBR) behavior and microbial community changes. Sci. Total Environ., 672, 227-238 (2019).
  • Takeuchi, M., Ozaki, H., Hiraoka, S., Kamagata, Y., Sakata, S., Yoshioka, H., Iwasaki, W.: Possible cross-feeding pathway of facultative methylotroph Methyloceanibacter caenitepidi Gela4 on methanotroph Methylocaldum marinum S8. PLoS One (2019).
  • Iwasaki, M., Miyake, M., Maseda, H., Qi, G., Pan, Z., Ihara, I., Umetsu, K.: Thermophilic anaerobic digestion is an effective treatment for reducing cefazolin-resistant bacteria and ESBL-producers in dairy manure. J. Mater. Cycles Waste Manag., 1-7 (2018).
  • Fukuda, N., Senga, Y., Honda, S.: Anxa2‐ and Ctsd‐knockout CHO cell lines to diminish the risk of contamination with host cell proteins. Biotechnol. Prog. (2019).
  • Fukuda, N., Honda, S.: Artificial mating-type conversion and repetitive mating for polyploid generation. ACS Synth. Biol., 7(5), 1413-1423 (2018).
  • Fukuda, N., Kaishima, M., Ishii, J., Honda, S.: Positive detection of GPCR antagonists using a system for inverted expression of a fluorescent reporter gene. ACS Synth. Biol. (2017).
  • Kaishima, M., Ishii, J., Matsuno, T., Fukuda, N., Kondo, A.: Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity. Sci. Rep., 6, 35932 (2016).
  • Fukuda, N., Kaishima, M., Ishii, J., Kondo, A., Honda, S.: Continuous crossbreeding of sake yeasts using growth selection systems for a-type and α-type cells. AMB Expr., 6,45 (2016).
  • Wu, C.: Biotinylation of deoxyguanosine at the abasic site in double-stranded oligodeoxynucleotides. J. Anal. Methods Chem., 2016 (2016).
  • Kaishima, M., Ishii, J., Fukuda, N., Kondo, A.: Gγ recruitment systems specifically select PPI and affinity-enhanced candidate proteins that interact with membrane protein targets. Sci. Rep., 5, 16723 (2015).
  • Fukuda, N., Honda, S.: Rapid evaluation of tyrosine kinase activity of membrane-integrated human epidermal growth factor receptor using the yeast Gγ recruitment system. ACS Synth. Biol., 4(4), 421-429 (2015).
  • Fukuda, N., Honda, S.: Functional analyses and affinity-alteration of receptors and enzymes based on membrane recruitment of yeast guanine nucleotide-binding protein gamma subunit. Receptors & Clinical Investigation, 2(1), 373 (2015).

部門長・副部門長・首席研究員・総括研究員・招聘研究員
Director, Deputy Director, Prime Senior Researcher, Principal Research Manager, Honda Lab.

  • Watthaison, P., Pongpamorn, P., Pimviriyakul, P., Maenpuen, S., Ohmiya, Y., Chaiyen, P.: A Chemo-Enzymatic Cascade for the Smart Detection of Nitro- and Halogenated Phenols. Angewandte Chemie. 131(38), 13388-13392 (2019).
  • Zheng, Y., Saitou, A., Wang, C., Toyoda, A., Minakuchi, Y., Sekiguchi, Y., Ueda, K., Ueda, K., Takano, H., Sakai, Y., Abe, K., Yokota, A., Yabe, S.: Genome features and secondary metabolites biosynthetic potential of the glass Ktedonobacteria. Front. Microbiol., 10, 893 (2019).
  • Shibuya, R., Miyafusa, T., Honda, S.: Stabilization of backbone‐circularized protein is attained by synergistic gains in enthalpy of folded structure and entropy of unfolded structure. FEBS J., 15092 (2019).
  • Yageta, S., Imamura, H., Shibuya, R., Honda, S.: CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering. mAbs, 11(3), 453-462 (2019).
  • Yageta, S., Imamura, H., Shibuya, R., Honda, S.: C H 2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering. mAbs (2018)
  • Waite, D.W., Dsouza, M., Sekiguchi, Y., Hugenholtz, P., Taylor, M.W.: Network-guided genomic and metagenomic analysis of the faecal microbiota of the critically endangered kakapo. Sci. Rep., 8, 8128 (2018).
  • Imamura, H., Sasaki, A., Honda, S.: Fate of a stressed therapeutic antibody tracked by fluorescence correlation spectroscopy: Folded monomers survive aggregation. J. Phys. Chem. B, 121(34), 8085-8093 (2017).
  • Torisu, T., Maruno, T., Yoneda, S., Hamaji, Y., Honda, S., Ohkubo, T., Uchiyama, S.: Friability testing as a new stress-stability assay for biopharmaceuticals. J. Pharm. Sci. (2017).
  • Yageta, S., Shibuya, R., Imamura, H., Honda, S.: Conformational and colloidal stabilities of human immunoglobulin G Fc and its cyclized variant: Independent and compensatory participation of domains in aggregation of multi-domain proteins. Mol. Pharm. (2017).
  • Imamura, H., Honda, S.: Calibration-free concentration analysis for an analyte prone to self-association. Anal. Biochem., 516, 61-64 (2017).
  • Furutani, S., Naruishi, N., Hagihara, Y., Nagai, H.: Development of an on-site rapid real-time polymerase chain reaction system and the characterization of suitable DNA polymerases for TaqMan probe technology. Anal. Bioanal. Chem., 408(20), 5641-5649 (2016).
  • Imamura, H., Honda, S.: Surface plasmon resonance biosensing of the monomer and the linked dimer of the variants of protein G under mass transport limitation. Data Brief, 9, 917-921 (2016).
  • Imamura, H., Honda, S.: Kinetics of antibody aggregation at neutral pH and ambient temperatures triggered by temporal exposure to acid. J. Phys. Chem. B, 120(36), 9581-9589 (2016).
  • Miyahara, D., Oishi, I., Makino, R., Kurumisawa, N., Nakaya, R., Ono, T., Kagami, H., Tagami, T.: Chicken stem cell factor enhances primordial germ cell proliferation cooperatively with fibroblast growth factor 2. J. Reprod. Dev., 2015, 128 (2016).
  • Oishi, I., Yoshii, K., Miyahara, D., Kagami, H., Tagami, T.: Targeted mutagenesis in chicken using CRISPR/Cas9 system. Sci Rep, 23980 (2016).
  • Thandar, SM., Ushiki, N., Fujitani, H., Sekiguchi, Y., Tsuneda, S.: Ecophysiology and comparative genomics of nitrosomonas mobilis Ms1 isolated from autotrophic nitrifying granules of wastewater treatment bioreactor. Front. Microbiol., 7, 1869 (2016).
  • Noguchi, M., Kurisu, F., Sekiguchi, Y., Kasuga, I., Furumai, H.: Microbial community structure of methanogenic benzene-degrading cultures enriched from five different sediments. J. Gen. Appl. Microbiol. (2016).
  • Sun, L., Toyonaga, M., Ohashi, A., Matsuura, N., Tourlousse, D.M., Meng, X.Y., Tamaki, H., Hanada, S., Cruz, R., Yamaguchi, T., Sekiguchi, Y.: Isolation and characterization of Flexilinea flocculare gen. nov., sp. nov., a filamentous anaerobic bacterium belonging to the class Anaerolineae in the phylum Chloroflexi. Int. J. Syst. Evol. Microbiol. (2016).
  • Yeoh, Y.K., Sekiguchi, Y., Parks, D.H., Hugenholtz, P.: Comparative genomics of candidate phylum TM6 suggests that parasitism is widespread and ancestral in this lineage. Mol. Biol. Evol. (2016).
  • Matsuura, N., Ohashi, A., Toulousse, D.M., Sekiguchi, Y.: Draft genome sequence of thermodesulfovibrio aggregans TGE-P1T, an obligately anaerobic, thermophilic, sulfate-reducing bacterium in the phylum nitrospirae. Genome Announc., 4(2), e00089-16 (2016).
  • Matsuura, N., Ohashi, A., Toulousse, D.M., Sekiguchi, Y.: Draft genome sequence of the syntrophic lactate-degrading bacterium tepidanaerobacter syntrophicus JLT. Genome Announc., 4(1), e01712-15 (2016).
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