多細胞システム制御研究グループ

研究課題

糖鎖は全ての細胞の最も外側を覆い、細胞の種類や状態（分化、がん化）で変化することから「細胞の顔」と呼ばれ、再生医療に用いる細胞の品質管理や、がん等の疾患の創薬標的として期待されています。我々はこれまで組換えレクチンライブラリーを構築し、これらを用いることで糖鎖をプロファイリングする技術の開発に取り組んできました。まず複数のレクチンをスライドグラス上に固定化したレクチンアレイの開発と利用を進めてきました。また最近、シーケンシングによる糖鎖プロファイリング技術を開発し、１細胞ごとの糖鎖発現と遺伝子発現を同時解析する技術(scGR-seq)の確立に展開することに成功しました。さらに微生物叢の糖鎖プロファイリングにも応用しました。本技術の更なる高度化と応用を進めていきます。

研究課題

ヒト多能性細胞やヒト間葉系幹細胞は再生医療のための細胞源として期待されています。これら細胞をヒトに移植する際、細胞の安全性や品質の確保が極めて重要です。我々はこれまで、移植用細胞に残存する造腫瘍性のヒト多能性幹細胞を非破壊的に検出し、除去する技術を開発し、企業と共同で実用化することに成功しました。今後も新しい品質管理技術の開発を目指します。

研究課題

がん、糖尿病、精神・神経疾患の診断技術の開発は、確定診断・治療方針の決定・QOLの向上・早期の社会復帰のために重要です。我々は正常細胞と疾患細胞（前立腺がん、乳がん、膵癌、糖尿病、精神疾患）の細胞機能や遺伝子・糖鎖発現を比較解析することで、新規の診断技術の開発を目指しています。

研究課題

エクソソームは真核細胞のエンドソーム区画で産生される細胞膜結合性の細胞外小胞であり、体循環に乗って他の細胞へと運ばれ、発生、生理、病理的プロセスにおいて細胞間コミュニケーションツールとして機能しています。所内外と連携し、細胞から分泌された直後のインタクトな状態にある膜小胞の調製技術の高度化、活用技術の開発、糖鎖機能解明など、理工学的視点からナノサイズの未開拓領域である謎多きエクソソームの本質の解明に挑戦します。

研究課題

近年、マイクロ波は化学反応の効率加熱手段として多用されるが、低温での反応や精密な温度制御が要求される糖鎖や創薬活性物質の精密合成での利用展開は進んでいない。また、化学反応におけるマイクロ波の加熱特性以外の特有効果については疑問視する声も多く、 「まやかし」とも揶揄する学者も存在する。
我々は、１）冷却機能を備えたマイクロ波照射反応装置を製作し、精密な温度制御や低温での照射を可能とし、２）LeX３糖体合成やペプチド合成に適応させ、マイクロ波特有の効果を明確にした。そして３）高価で貴重な糖アミノ酸シントンの安定性と反応性両方の向上に成功し、世界に類のない複雑な糖鎖ペプチドの数十mgスケール合成法の確立、４）酵素反応を題材としてマイクロ波の分子選択活性化効果を考察し、マイクロ波効果の本質に迫る、などの研究をすすめている。

業績リスト

• Nie, H; Saini, P; Miyamoto, T; Liao, L; Zielinski, RJ; Liu, H; Zhou, W; Wang, C; Murphy, B; Towers, M; Yang, T; Qi, Y; Kannan, T; Kossenkov, A; Tateno, H; Claiborne, DT; Zhang, N; Abdel-Mohsen, M; Zhang, R.
  Targeting branched N-glycans and fucosylation sensitizes ovarian tumors to immune checkpoint blockade.
  NAT COMMUN. 2024 Apr 2;15(1):2853. doi: 10.1038/s41467-024-47069-y
• Arai, J; Hayakawa, Y; Tateno, H; Murakami, K; Hayashi, T; Hata, M; Matsushita, Y; Kinoshita, H; Abe, S; Kurokawa, K; Oya, Y; Tsuboi, M; Ihara, S; Niikura, R; Suzuki, N; Iwata, Y; Shiokawa, T; Shiomi, C; Uekura, C; Yamamoto, K; Fujiwara, H; Kawamura, S; Nakagawa, H; Mizuno, S; Kudo, T; Takahashi, S; Ushiku, T; Hirata, Y; Fujii, C; Nakayama, J; Shibata, S; Woods, S; Worthley, DL; Hatakeyama, M; Wang, TC; Fujishiro, M.
  Impaired glycosylation of gastric mucins drives gastric tumorigenesis and serves as a novel therapeutic target.
  GASTROENTEROLOGY. 2024 Apr 5:S0016-5085(24)00363-9. doi: 10.1053/j.gastro.2024.03.037
• Miura, I; Shams, F; Ohki, J; Tagami, M; Fujita, H; Kuwana, C; Nanba, C; Matsuo, T Ogata, M; Mawaribuchi, S; Shimizu, N; Ezaz, T.
  Multiple Transitions between Y Chromosome and Autosome in Tagos Brown Frog Species Complex.
  GENES (Basel). 2024 Feb 26;15(3):300. doi: 10.3390/genes15030300
• Nakahashi, H; Oda, T; Shimomura, O; Akashi, Y; Takahashi, K; Miyazaki, Y; Furuta, T; Kuroda, Y; Louphrasitthiphol, P; Mathis, BJ; Tateno, H.
  Aberrant Glycosylation in Pancreatic Ductal Adenocarcinoma 3D Organoids Is Mediated by KRAS Mutations.
  J ONCOL. 2024 Mar 18;2024:1529449. doi: 10.1155/2024/1529449
• Nagashima, I; Shimizu, H.
  Preparation of Mucin Glycopeptides by Organic Synthesis.
  METHODS MOL BIOL. 2024;2763:187-199. doi: 10.1007/978-1-0716-3670-1_16
• Chen, LM; Keisham, S; Tateno, H; van, Ede J; Pronk, M; van, Loosdrecht MCM; Lin, Y.
  Alterations of Glycan Composition in Aerobic Granular Sludge during the Adaptation to Seawater Conditions.
  ACS ES T WATER. 2023 Dec 27;4(1):279-286. doi: 10.1021/acsestwater.3c00625
• Keisham, S; Saito, S; Kowashi, S; Tateno, H.
  Droplet-Based Glycan and RNA Sequencing for Profiling the Distinct Cellular Glyco-States in Single Cells.
  SMALL METHODS. 2024 Jan 2:e2301338. doi: 10.1002/smtd.202301338
• Yonezawa, A; Mizushima, K; Hirai, Y; Tateno, H; Muto, A; Igarashi, K; Takag, T; Naito, Y; Higashimura, Y.
  Involvement of transcription repressor Bach1 in function of intestinal goblet cells.
  ANNALS OF NUTRITION AND METABOLISM. 2023 ;79 (suppl 1) 1170-1170; AUG. doi: 10.1159/000530786
• Ishii, N; Noguchi, K; Ikemoto, MJ; Yohda, M; Odahara, T.
  Optimizing Exosome Preparation Based on Size and Morphology: Insights From Electron Microscopy.
  MICROSC MICROANAL. 2023 Oct 13:ozad103. doi: 10.1093/micmic/ozad103
• Ishii, N; Odahara, T.
  Investigation of the Efficacy of Lanthanoid Heavy Metal Acetates as Electron Staining Reagents for Biomembrane Vesicles.
  MICROSC MICROANAL. 2023 Oct 13:ozad107. doi: 10.1093/micmic/ozad107
• Odaka, H.
  Target-Specific O-linked N-acetylglucosamine Modification with a Dual-Specificity Aptamer Reveals Functions of O-GlcNAcylation in beta-catenin.
  TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY. May 25, 2023; Volume 35(2023) Issue 205. doi: 10.4052/tigg.2309.6E
• Watanabe, T, Tateno, H.
  Lectin Blotting of Cell Lysates.
  CURR PROTOC. 2023 May;3(5):e790. doi: 10.1002/cpz1.790
• Kuroda, Y; Oda, T; Shimomura, O; Louphrasitthiphol, P; Mathis, BJ; Tateno, H, Hatano, K.
  Novel positron emission tomography imaging targeting cell surface glycans for pancreatic cancer: (18) F-labeled rBC2LCN lectin.
  CANCER SCI. 2023 May 19. doi: 10.1111/cas.15846
• Odaka, H; Tateno, H.
  Single-Cell Glycan and RNA Sequencing (scGR-seq).
  CURR PROTOC. 2023 May;3(5):e777. doi: 10.1002/cpz1.777
• Ikemoto, MJ; Aihara, Y; Ishii, N; Shigemori, H.
  3,4-Dihydroxybenzalacetone Inhibits the Propagation of Hydrogen Peroxide-Induced Oxidative Effect via Secretory Components from SH-SY5Y Cells.
  BIOL PHARM BULL. 2023;46(4):599-607. doi: 10.1248/bpb.b22-00727
• Murata, Y; Jitsukawa, T; Iida, M; Furuta, T; Haramoto, Y; Shigeri, Y; Fujisaki, S.
  Liquid chromatography-mass spectrometry analyses of polyprenyl phosphates in Escherichia coli cells in which genes for isoprenoid synthesis are amplified.
  J BIOSCI BIOENG. 2023 Mar 1:S1389-1723(23)00047-6. doi: 10.1016/j.jbiosc.2023.02.003
• Mawaribuchi, S; Ito, M; Ogata, M; Yoshimura, Y; Miura, I.
  Parallel Evolution of Sex-Linked Genes across XX/XY and ZZ/ZW Sex Chromosome Systems in the Frog Glandirana rugosa.
  GENES (Basel). 2023 Jan 18;14(2):257. doi: 10.3390/genes14020257
• Sato, Y; Kato, Y; Iizumi, Y; Morimoto, T; Kobashi, K; Sugino, T; Tateno, H; Okazaki, T.
  Size distributions of cellulose nanocrystals in dispersions using the centrifugal sedimentation method.
  INT J BIOL MACROMOL. 2023 Feb 2:123520. doi: 10.1016/j.ijbiomac.2023.123520
• Mawaribuchi, S; Shimomura, O; Oda, T; Hiemori, K; Shimizu, K; Yamase, K; Date, M; Tateno, H.
  rBC2LCN-reactive SERPINA3 is a glycobiomarker candidate for pancreatic ductal adenocarcinoma.
  GLYCOBIOLOGY. 2023 Feb 2:cwad009. doi: 10.1093/glycob/cwad009
• Huang, T; Sato, Y; Kuramochi, A; Ohba, Y; Sano, M; Miyagishi, M; Tateno, H; Wadhwa, R; Kawasaki, K; Uchida, T; Ekdahl, KN; Nilsson, B; Chung, UI; Teramura, Y.
  Surface modulation of extracellular vesicles with cell-penetrating peptide-conjugated lipids for improvement of intracellular delivery to endothelial cells.
  REGEN THER. 2023 Jan 11;22:90-98. doi: 10.1016/j.reth.2022.12.007
• Mawaribuchi, S; Haramoto, Y; Ikeda, N; Ito, M.
  Evolutionary features of ligands and their receptors via protein-protein interactions and essentiality in primates.
  GENES CELLS. 2023 Jan 9. doi: 10.1111/gtc.13006
• Ishii, N.
  C-shaped dipper: A novel useful auxiliary tool for preparation of specimen grids for transmission electron microscopy.
  MICROSC RES TECH. 2022 Dec 29. doi: 10.1002/jemt.24283
• Ando, M; Kawasaki, H; Tamura, S; Haramoto, Y; Shigeri, Y.
  Recent Advances in Gas Sensing Technology Using Non-Oxide II-VI Semiconductors CdS, CdSe, and CdTe.
  CHEMOSENSORS. 2022 10(11)482. doi: 10.3390/chemosensors10110482
• Nagashima, I; Sugiyama, JI; Shimizu, H.
  Study of 400 MHz microwave conduction loss effect for a hydrolysis reaction by thermostable β-Glucosidase HT1.
  BIOSCI BIOTECHNOL BIOCHEM. 2022 Dec 6:zbac194. doi: 10.1093/bbb/zbac194
• Kuroda, Y; Shimomura, O; Nakahashi, H; Miyazaki, Y; Nakajima, T; Tateno, H; Oda, T.
  Lectin-based photoimmunotherapy targeting cell surface glycans for pancreatic cancer.
  CANCER RESEARCH 82 (22). 2022 NOV 15. doi: 10.1158/1538-7445.PANCA22-B083
• Kuroda, Y; Oda, T; Shimomura, O; Hashimoto, S; Akashi, Y; Miyazaki, Y; Furuya, K; Furuta, T; Nakahashi, H; Louphrasitthiphol, P; Mathis, BJ; Nakajima,T; Tateno, H.
  Lectin-based phototherapy targeting cell surface glycans for pancreatic cancer.
  INT J CANCER. 2022 Nov 22. doi: 10.1002/ijc.34362
• Tasaki, M; Tateno, H; Sato, T; Narimatsu, H; Saito, K; Nakagawa, Y; Aoki, T; Kamimura, M; Ushiki, T; Takahashi, K; Tomita, Y.
  Hyporesponsiveness against donors ABO antigens of renal grafts after ABO-incompatible kidney transplantation.
  CLIN EXP NEPHROL. 2022 Oct 8. doi: 10.1007/s10157-022-02280-3
• Kosaka, T; Yamaguchi, S; Izuta, S; Yamahira, S; Shibasaki, Y; Tateno, H; Okamoto, A.
  Bioorthogonal Photoreactive Surfaces for Single-Cell Analysis of Intercellular Communications.
  AM CHEM SOC. 2022 Sep 20. doi: 10.1021/jacs.2c07321
• Yamasaki, K; Adachi, N; Ngwe Tun, MM; Ikeda, A; Moriya, T; Kawasaki, M; Yamasaki, T; Kubota, T; Nagashima, I; Shimizu, H; Tateno, H; Morita, K.
  Core fucose-specific Pholiota squarrosa lectin (PhoSL) as a potent broad-spectrum inhibitor of SARS-CoV-2 infection.
  FEBS J. 2022 Aug 25. doi: 10.1111/febs.16599
• Katsumi, T; Shams, F; Yanagi, H; Ohnishi, T; Toda, M; Lin, SM; Mawaribuchi, S; Shimizu, N; Ezaz, T; Miura, I.
  Highly rapid and diverse sex chromosome evolution in the Odorrana frog species complex.
  DEV GROWTH DIFFER. 2022 Jul 26. doi: 10.1111/dgd.12800
• Numakawa, T; Odaka, H.
  The Role of Neurotrophin Signaling in Age-Related Cognitive Decline and Cognitive Diseases.
  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022 Jul 13;23(14):7726. doi: 10.3390/ijms23147726
• Kokubu, R; Ohno, S; Kuratani, H; Takahashi, Y; Manabe, N; Shimizu, H; Chiba, Y; Denda-Nagai, K; Tsuiji, M; Irimura, T; Yamaguchi, Y.
  O-Glycan-Dependent Interaction between MUC1 Glycopeptide and MY.1E12 Antibody by NMR, Molecular Dynamics and Docking Simulations.
  INT J MOL SCI. 2022 Jul 16;23(14):7855. doi: 10.3390/ijms23147855
• Nagae, M; Hirata, T; Tateno, H; Mishra, SK; Manabe, N; Osada, N; Tokoro, Y; Yamaguchi, Y; Doerksen, RJ; Shimizu, T; Kizuka, Y.
  Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A).
  COMMUN BIOL. 2022 Jul 19;5(1):695. doi: 10.1038/s42003-022-03661-w
• Odaka, H.
  Genome-wide CRISPR/Cas9 Screening Identifies KDM2B as a Modifier of Heparan Sulfate-Protein Interactions.
  TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY. 2022 Volume 34 Issue 199 Pages E63. doi: 10.4052/tigg.2203.6E
• Tsukano, K; Yamamoto, T; Watanabe, T; Michiue, T.
  Xenopus Dusp6 modulates FGF signaling to precisely pattern pre-placodal ectoderm.
  DEVELOPMENTAL BIOLOGY. 2022 May 16. doi: 10.1016/j.ydbio.2022.05.009
• Oinam, L; Tateno, H.
  Glycan Profiling by Sequencing to Uncover Multicellular Communication: Launching Glycobiology in Single Cells and Microbiomes.
  FRONT CELL DEV BIOL. 2022 May 27;10:919168. doi: 10.3389/fcell.2022.919168
• Yang, Y; Akashi, Y; Shimomura, O; Tateno, H; Saito, S; Hiemori, K; Miyazaki, Y; Furuta, T; Kitaguchi, D; Kuroda, Y; Pakavarin, L; Oda, T.
  Glycan expression profile of signet ring cell gastric cancer cells and potential applicability of rBC2LCN-targeted lectin drug conjugate therapy.
  GASTRIC CANCER. 2022 Jun 17. doi: 10.1007/s10120-022-01312-x
• Miura, I; Shams, F; Jeffries, DL; Katsura, Y; Mawaribuchi, S; Perrin, N; Ito, M; Ogata, M; Ezaz, T.
  Identification of ancestral sex chromosomes in the frog Glandirana rugosa bearing XX-XY and ZZ-ZW sex-determining systems.
  MOL ECOL. 2022 Jun 12. doi: 10.1111/mec.16551
• Odaka, H; Hiemori, K; Shimoda, A; Akiyoshi, K; Tateno, H.
  CD63-positive extracellular vesicles are potential diagnostic biomarkers of pancreatic ductal adenocarcinoma.
  BMC GASTROENTEROLOGY. 2022 Mar 28;22(1):153. doi: 10.1186/s12876-022-02228-7
• Hayashi, Y; Yimiti, D; Sanada, Y; Ding, C; Omoto, T; Ogura, T; Nakasa, T; Ishikawa, M; Hiemori, K; Tateno, H; Miyaki, S; Adachi, N.
  The therapeutic capacity of bone marrow MSC-derived extracellular vesicles in Achilles tendon healing is passage-dependent and indicated by specific glycans.
  FEBS LETTERS. 2022 Apr;596(8):1047-1058. doi: 10.1002/1873-3468.14333
• Tasaki, M; Tateno, H; Sato, T; Tomioka, A; Kaji, H; Narimatsu, H; Saito, K; Nakagawa, Y; Aoki, T; Kamimura, M; Ushiki, T; Okada, M; Miwa, Y; Hotta, K; Yoshida, Y; Takahashi, K; Tomita, Y.
  A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation.
  TRANSPL INT. 2022 Mar 23;35:10248. doi: 10.3389/ti.2022.10248
• Oinam, L; Hayashi, R; Hiemori, K; Kiyoi, K; Sage-Ono, K; Miura, K; Ono, M; Tateno, H.
  Quantitative evaluation of glycan-binding specificity of recombinant Concanavalin A produced in lettuce (Lactuca sativa).
  BIOTECHNOL BIOENG. 2022 Apr 8. doi: 10.1002/bit.28099
• Ishii, N.
  Systematic Investigation of Lanthanoid Transition Heavy Metal Acetates as Electron Staining Reagents for Protein Molecules in Biological Transmission Electron Microscopy.
  MICROSC MICROANAL. 2022 Apr 1:1-10. doi: 10.1017/S1431927622000411
• Seo, N; Nakamura, J; Kaneda, T; Tateno, H; Shimoda, A; Ichiki, T; Furukawa, K; Hirabayashi, J; Akiyoshi, K; Shiku, H.
  Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method.
  J EXTRACELL VESICLES. 2022 Mar;11(3):e12205. doi: 10.1002/jev2.12205
• Odaka, H; Ozaki, H; Tateno, H.
  scGR-seq: Integrated analysis of glycan and RNA in single cells.
  STAR PROTOC. 2022 Feb 18;3(1):101179. doi: 10.1016/j.xpro.2022.101179. eCollection
• Irumagawa, S; Hiemori, K; Saito, S; Tateno, H; Arai, R.
  Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans.
  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23 (2);doi: 10.3390/ijms23020676 JAN
• Oinam, L; Tateno, H.
  Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System.
  METHODS MOL BIOL. 2022;2460:25-32. doi: 10.1007/978-1-0716-2148-6_2
• Kaifu, T; Yabe, R; Maruhashi, T; Chung, SH; Tateno, H; Fujikado, N; Hirabayashi, J; Iwakura, Y.
  DCIR and its ligand asialo-biantennary N-glycan regulate DC function and osteoclastogenesis.
  J EXP MED. 2021 Dec 6;218(12):e20210435. doi: 10.1084/jem.20210435. Epub 2021 Nov
• Shimoda, A; Miura, R; Tateno, H; Seo, N; Shiku, H; Sawada, S; Sasaki, Y; Akiyoshi, K.
  Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications.
  SMALL METHODS; doi: 10.1002/smtd.202100785
• Tulin, EKC; Nakazawa, C; Nakamura, T; Saito, S; Ohzono, N; Hiemori, K; Nakakita, SI; Tateno, H; Tonozuka, T; Nishikawa, A.
  Glycan detecting tools developed from the Clostridium botulinum whole hemagglutinin complex.
  SCI REP. 2021 Nov 9;11(1):21973. doi: 10.1038/s41598-021-01501-1
• Kaigawa, H; Tateno, H; Arai, R.
  Self-Assembling Functional Complexes of Protein Nanobuilding Blocks with a Trimeric Lectin.
  PROTEIN SCIENCE 30 158-159; OCT
• Irumagawa, S; Tateno, H; Arai, R.
  Self-Assembling Lectin Nano-Building Blocks Enhance Avidity to Glycans through Polyvalent Effect.
  PROTEIN SCIENCE 30 173-174; OCT
• Hoshi, K; Ito, H; Abe, E; Fuwa, TJ; Kanno, M; Murakami, Y; Abe, M; Murakami, T, Yoshihara, A, Ugawa, Y, Saito, T, Saido, TC, Matsumoto, K, Yamaguchi, Y, Furukawa, K, Arai, H, Kanai, M, Miyajima, M, Arai, H, Ogawa, N, Akatsu, H, Hashizume, Y, Tateno, H, Honda, T, Hashimoto, Y.
  Transferrin Biosynthesized in the Brain Is a Novel Biomarker for Alzheimers Disease.
  METABOLITES. 2021 Sep 10;11(9):616. doi: 10.3390/metabo11090616
• Minoshima, F; Ozaki, H; Odaka, H; Tateno, H.
  Integrated analysis of glycan and RNA in single cells.
  ISCIENCE. 2021 Jul 17;24(8):102882. doi: 10.1016/j.isci.2021.102882. eCollection
• Watanabe, T; Takeda, K; Hiemori, K; Minamisawa, T; Tateno, H.
  A glycosaminoglycan microarray identifies the binding of SARS-CoV-2 spike protein to chondroitin sulfate E.
  FEBS LETT. 2021 Aug 10. doi: 10.1002/1873-3468.14173
• Haramoto, Y.
  First person - Yoshikazu Haramoto.
  BIOLOGY OPEN 10 (4);doi: 10.1242/bio.058759 APR
• Ogita, Y; Tamura, K; Mawaribuchi, S; Takamatsu, N; Ito, M.
  Independent pseudogenizations and losses of sox15 during amniote diversification following asymmetric ohnolog evolution.
  BMC ECOL EVOL. 2021 Jun 30;21(1):134. doi: 10.1186/s12862-021-01864-z
• Furuta, T; Oda, T; Kiyoi, K; Ozawa, Y; Kimura, S; Kurimori, K; Miyazaki, Y; Yu, Y; Furuya, K; Akashi, Y; Shimomura, O; Tateno, H
  Carcinoembryonic antigen as a specific glycoprotein ligand of rBC2LCN lectin on pancreatic ductal adenocarcinoma cells
  Cancer Sci. 2021 Jun 11. doi: 10.1111/cas.15023
• Numakawa, T; Odaka, H.
  Brain-Derived Neurotrophic Factor Signaling in the Pathophysiology of Alzheimers Disease: Beneficial Effects of Flavonoids for Neuroprotection.
  INT J MOL SCI. 2021 May 27;22(11):5719. doi: 10.3390/ijms22115719
• Haramoto, Y; Sakata, M; Kobayashi, S.
  Visualization of X chromosome reactivation in mouse primordial germ cells in vivo.
  BIOL OPEN. 2021 Apr 15;10(4):bio058602. doi: 10.1242/bio.058602. Epub 2021 Apr 29
• Watanabe, T; Tateno, H.
  Elimination of cells deviated from human induced pluripotent stem cells with a photoactivatable IR700-labelled antibody.
  BIOCHEM BIOPHYS RES COMMUN. 2021 Mar 25;554:13-18. doi: 10.1016/j.bbrc.2021.03.078
• Furuta T, Tateno H, Kiyoi K, Miyazaki Y, Kimura S, Kurimori K, Shimomura O, Oda T
  Glycans on CEA as a targeting ligand of pancreatic ductal adenocarcinoma in novel lectin-drug conjugated therapy.
  CANCER SCIENCE 112 718-718; FEB
• Odaka H, Numakawa T, Soga M, Kido J, Matsumoto S, Kajihara R, Okumiya T, Tani N, Tanoue Y, Fukuda T, Furuya H, Inoue T, Era T
  An iPSC-based neural model of sialidosis uncovers glycolytic impairment-causing presynaptic dysfunction and deregulation of Ca(2+) dynamics
  Neurobiol Dis. 2021 Jan 28:105279. doi: 10.1016/j.nbd.2021.105279
• Kimura S, Oda T, Shimomura O, Enomoto T, Hashimoto S, Kuroda Y, Yu Y, Kurimori K, Furuta T, Miyazaki Y, Tateno H
  Novel Pancreatic Cancer Therapy Targeting Cell Surface Glycans by Liposomes Modified with rBC2LCN Lectin
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  Platelet-derived extracellular vesicles are increased in sera of Alzheimers disease patients, as revealed by Tim4-based assays.
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  Complete mitochondrial genome sequence of Japanese forest green tree frog (Rhacophorus arboreus)
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  Monoclonal antibodies specific for podocalyxin expressed on human induced pluripotent stem cells.
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  Interferon-α alters host glycosylation machinery during treated HIV infection
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  SSEA-1-positive fibronectin is secreted by cells deviated from the undifferentiated state of human induced pluripotent stem cells
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  Glycome profiling by lectin microarray reveals dynamic glycan alterations during epidermal stem cell aging
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  rBC2LCN lectin as a potential probe of early-stage HER2-positive breast carcinoma
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  Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection
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  Plasma and antibody glycomic biomarkers of time to HIV rebound and viral setpoint
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  Glycan Binding Profiling of Jacalin-Related Lectins from the Pteria Penguin Pearl Shell
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  Structural basis for specific recognition of core fucosylation in N-glycans by Pholiota squarrosa lectin (PhoSL
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  Identification of Aquatic Organisms Using a Magneto-Optical Element
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  Characterization and functional analysis of novel circulating NK cell sub-populations
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  Human Stem Cell Glycome: From Structural Elucidation to Social Implementation
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  Photoactivable Elimination of Tumorigenic Human Induced Pluripotent Stem Cells by Using a Lectin-Doxorubicin Prodrug Conjugate
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  Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis
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  Chromosome alignment maintenance requires the MAP RECQL4, mutated in the Rothmund-Thomson syndrome
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  Fucose-specific lectin of Aspergillus fumigatus: binding properties and effects on immune response stimulation
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  Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Lectin AJLec from the Sea Anemone Anthopleura japonica
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  The trimeric solution structure and fucose-binding mechanism of the core fucosylation-specific lectin PhoSL
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  Investigation of Selective Recognition of Sugars Using Lectin-inspired Temperature-responsive Polymers
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  A Novel Therapeutic Strategy for Pancreatic Cancer: Targeting Cell Surface Glycan Using rBC2LC-N Lectin-Drug Conjugate (LDC
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  Structural and quantitative evidence of alpha2-6-sialylated N-glycans as markers of the differentiation potential of human mesenchymal stem cells
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  Distinct roles for each N-glycan branch interacting with mannose-binding type Jacalin-related lectins Orysata and Calsepa
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  Development of a Sensitive Microarray Platform for the Ranking of Galectin Inhibitors: Identification of a Selective Galectin-3 Inhibitor
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  Diabetes-Induced Dysfunction of Mitochondria and Stem Cells in Skeletal Muscle and the Nervous System
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  Diabetes-Induced Dysfunction of Mitochondria and Stem Cells in Skeletal Muscle and the Nervous System
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  Lectin microarray analysis of isolated polysaccharides from Sasa veitchii
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  Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus
  Protein Science 26: 1574-1583 (2017) doi: 10.1002/pro.3185
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  Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells
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  Identification and comparative analyses of Siamois cluster genes in Xenopus laevis and tropicalis
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  Sugar-Binding Profiles of Chitin-Binding Lectins from the Hevein Family: A Comprehensive Study
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  Engineering of recombinant Wisteria floribunda agglutinin specifically binding to GalNAcbeta1,4GlcNAc (LacdiNAc
  Glycobiology. 0: 0 (2017) doi: 10.1093/glycob/cwx038
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  Isolation of Rice Bran Lectins and Characterization of Their Unique Behavior in Caco-2 Cells
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  Electron microscopic and physicobiochemical studies of chaperonin GroEL and GroES complexes.
  Microscopy and imaging science: practical approaches to applied research and education 7: 112-119 (2017)
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  GroEL and the GroEL-GroES Complex
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  Asymmetry in the function and dynamics of the cytosolic group II chaperonin CCT/TRiC
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