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バイオ変換グループは、化学品のバイオ化を支える酵素・微生物の開発を行っています。

過去の業績ACCOMPLISHMENT (PAST)

原著論文

2021年

Akita H, Itoiri Y , Takeda N, Kimura Zen-Ichiro, Inoue H, Matsushika A: Draft Genome Sequence of Klebsiella pneumoniae subsp. pneumoniae CCI2, Isolated from Leaf Soil.
Microbiol Resour Announc, 10(25):e0034321 (2021)

Akita H, Goshima T, Suzuki T, Itoiri Y, Kimura Z, Matsushika A: Application of Pichia kudriavzevii NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production.
Fermentation, 7(2): 10.3390/fermentation7020083

Akita H, Kumagai A: Biosorption of Palladium Using Enterobacter oligotrophicus CCA6T.
Salt and Seawater Science & Technology, 2:19-21 (2021).

2020年

Akita H, Itoiri Y, Ihara S, Takeda N, Matsushika A, Kimura Z: Deinococcus kurensis sp. nov., isolated from pond water collected in Japan.
Arch Microbiol, in press

Akita H, Itoiri Y, Kumagai A, Takeda N, Matsushika A, Oshiki M, Kimura Z: Draft genome sequence of Deinococcus sp. KR-1, a potential strain for palladium-leaching.
J Genomics 8, 21-24.

2019年

Nakamichi Y#, Fujii T#, Fouquet T, Matsushika A, Inoue H: GH30-7 endoxylanase C from the filamentous fungus Talaromyces cellulolyticus. Appl Environ Microbiol 85, e01442-19.

Nakamichi Y, Fouquet T, Ito S, Matsushika A, Inoue H: Mode of action of GH30-7 reducing-end xylose-releasing exoxylanase A (Xyn30A) from the filamentous fungus Talaromyces cellulolyticus. Appl Environ Microbiol 85, e00552-19.

Suzuki T, Hoshino T, Matsushika A: High-temperature ethanol production by a series of recombinant xylose-fermenting Kluyveromyces marxianus strains. Enzyme Microb Technol 129, 109359

Mine S and Watanabe M: Structural Insights into the Molecular Evolution of the Archaeal Exo-β-D-glucosaminidase. Int J Mol Sci. 20(10), 2460

Matsuzawa T, Watanabe M, Kameda T, Kameyama A, and Yaoi K: Cooperation between β-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae. FEBS J 286(16), 3182-3193

Akita H, Matsushika A, Kimura ZI: Enterobacter oligotrophica sp. nov., a novel oligotroph isolated from leaf soil. MicrobiologyOpen 8, e843

Nakabayashi M, Kamachi S, Malle D, Yanamoto T, Kishishita S, Fujii T, Inoue H, Ishikawa K: Construction of thermostable cellobiohydrolase I from the fungus Talaromyces cellulolyticus by protein engineering. Protein Eng Des Sel 32(1), 33-40

Nakamichi Y, Fouquet T, Ito S, Watanabe M, Matsushika A, Inoue H: Structural and functional characterization of a bifunctional GH30-7 xylanase B from the filamentous fungus Talaromyces cellulolyticus. J Biol Chem 294(11), 4065-4078

2018年

Fouquet T, Sato H, Nakamichi Y, Matsushika A, Inoue H: Electrospray multistage mass spectrometry in the negative ion mode for the unambiguous molecular and structural characterization of acidic hydrolysates from 4‐O‐methylglucuronoxylan generated by endoxylanases. J Mass Spectrom 54(3), 213-221

Matsuzawa T#, Watanabe M#, Nakamichi Y, Fujimoto Z, Yaoi K: Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme. J Struct Biol 205(1), 84-90

Yuzawa S, Mirsiaghi M, Jocic R, Fujii T, Masson F, Benites VT, Baidoo EEK, Sundstrom E, Tanjore D, Pray TR, George A, Davis RW, Gladden JM, Simmons BA, Katz L, Keasling JD: Short-chain ketone production by engineered polyketide synthases in Streptomyces albus. Nat Commun 9(1):4569

Nakamichi Y, Miyazaki N, Tsutsumi K, Higashiura A, Narita H, Murata K, Nakagawa A: An assembly intermediate structure of Rice Dwarf Virus reveals a hierarchical outer capsid shell assembly mechanism. Structure 27(3), 439-448

Akita H, Kimura ZI, Hoshino T: Pseudomonas humi sp. nov., isolated from leaf soil. Arch. Microbiol. 201(2), 245–251

Ahmad N, Zakaria MR, Mohd Yusoff MZ, Fujimoto S, Inoue H, Ariffin H, Hassan MA, Shirai Y: Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production. Molecules 23(6), E1310

Bunterngsook B, Laothanachareon T, Chotirotsukon C, Inoue H, Fujii T, Hoshino T, Roongsawang N, Kuboon S, Kraithong W, Techanan W, Kraikul N, Champreda V: Development of tailor-made synergistic cellulolytic enzyme system for saccharification of steam exploded sugarcane bagasse. J Biosci Bioeng 125(4), 390–396

Matsuzawa T#, Watanabe M#, Yaoi K: Crystal structure of Aspergillus oryzae isoprimeverose-producing enzyme: New insight into the recognition of disaccharide in glycoside hydrolase family 3 enzymes. submitted

Watanabe M#, Matsuzawa T#, Yaoi K: Rational protein design for thermostabilization of glycoside hydrolases based on structural analysis. Appl Microbiol Biotechnol 102(20), 8677-8684

2017年

Sornlake W, Rattanaphanjak P, Champreda V, Eurwilaichitr L, Kittisenachai S, Roytrakul S, Fujii T, Inoue H: Characterization of cellulolytic enzyme system of Schizophyllum commune mutant and evaluation of its efficiency on biomass hydrolysis. Biosci Biotechnol Biochem. 81(7), 1289–1299

Bunterngsook B, Laothanachareon T, Natrchalayuth S, Lertphanich S, Fujii T, Inoue H, Youngthong C, Chantasingh D, Eurwilaichitr L, Champreda V: Optimization of a minimal synergistic enzyme system for hydrolysis of raw cassava pulp. Rsc Adv. 7(76), 48444–48453

Akita H,Kimura Z,Matsushika A: Complete genome sequence of Ureibacillus thermosphaericus A1,a thermophilic bacillus isolated from compost. Genome Announc. 5(38), e00910-17

Yusoff MZM,Akita H,Mohd AH,Fujimoto S,Yoshida M,Nakashima N,Hoshino T: Production of acetoin from hydrothermally pretreated oil mesocarp fiber using metabolically engineered Escherichia coli in a bioreactor system. Bioresour Technol, 245(Pt A), 1040–1048

Kimura Z,Akita H: Comparison of excess sludge reduction effect of dichlorophenol isomers. J Pollut Ef f Cont, 5, 3

Akita H, Kimura Z, Yusoff MZM, Nakashima N, Hoshino T: Identification and characterization of Burkholderia multivorans CCA53. BMC Res Notes, 10(1), 249

Akita H, Nakashima N, Hoshino T: Production of D-lactate with pyruvate producing strain. Biosci Biotechnol Biochem. 81(7), 1452–1455

Hayashi J, Seto T, Akita H, Watanabe M, Hoshino T, Yoneda K, Ohshima T, and Sakuraba H: Structure based engineering of an artificially generated NADP+-dependent D-amino acid dehydrogenase. Appl Environ Microbiol, 83(11), e00491-17

Yuzawa S, Bailey CB, Fujii T, Jocic R, Barajas JF, Benites VT, Baidoo EEK, Chen Y, Petzold CJ, Katz L, Keasling JD: Heterologous Gene Expression of N-Terminally Truncated Variants of LipPks1 Suggests a Functionally Critical Structural Motif in the N-terminus of Modular Polyketide Synthase. ACS Chem Biol. 12(11), 2725-2729

Fujii T, Inoue H, Ishikawa K, Hoshino T: Deletion Analysis of GH7 Endoglucanase Gene (cel7B) Promoter Region in a Talaromyces cellulolyticus ligD-Disrupted Strain. Appl Biochem Biotechnol 183(4), 1516-1525

Iwasaki Y, Kita A Yoshida K, Tajima T, Yano S, Shou T, Saito M, Kato J, Murakami K, Nakashimada Y: Homolactic Acid Fermentation by the Genetically Engineered Thermophilic Homoacetogen Moorella thermoacetica ATCC 39073. Appl Environ Microbiol 83(8), e00247-17

Rahayu F, Kawai Y, Iwasakia Y, Yoshida K, Kita A, Tajima T, Kato J, Murakami K, Nakashimada Y: Thermophilic ethanol fermentation from lignocellulose hydrolysate by genetically engineered Moorella thermoacetica, Bioresour Technol

Matsuzawa T#, Watanabe M#, Yaoi K: Improved thermostability of a metagenomic glucose-tolerant β-glycosidase based on its crystal structure. Appl Microbiol Biotechnol. 101(23-24), 8353-8363

Mine S#, Watanabe M#, Kamachi S, Abe Y, Ueda T: The Structure of an Archaeal β-Glucosaminidase Provides Insight into Glycoside Hydrolase Evolution. J Biol Chem 292(12), 4996-5006

Matsushika A, Suzuki T, Goshima T, Hoshino T: Evaluation of Saccharomyces cerevisiae GAS1 with respect to its involvement in tolerance to low pH and salt stress. J Biosci Bioeng 124(2), 164-170

Kobayashi Y, Sahara T, Suzuki T, Kamachi S, Matsushika A, Hoshino T, Ohgiya S, Kamagata Y, Fujimori K: Genetic improvement of xylose metabolism by enhancing the expression of pentose phosphate pathway genes in Saccharomyces cerevisiae IR-2 for high-temperature ethanol production. J Ind Microbiol Biotechnol. 44(6), 879-891


2016年

Akita, H., Kimura, Z., Hoshino, T.: Draft genome sequence of Pseudomonas sp. strain CCA1, isolated from leaf soil, Genome Announc., 4(6), e01371-16

Akita, H., Kimura, Z., Yusoff, M.Z.M., Hoshino, T.: Isolation and preliminary characterization of Pseudomonas sp. strain CCA 1 from soil for ligninolytic activity, JSM Biotechnol. Bioeng., 3(4), 1061

Akita, H., Nakashima, N., Hoshino, T.: Pyruvate production using engineered Escherichia coli, AMB Express, 6(1), 94

Uechi, K.Kamachi, S.Akita, H., Mine, S., Watanabe, M.: Crystal structure of an acetyl esterase complexed with acetate ion provides insights into the catalytic mechanism, Biochem. Biophys. Res. Commun., 477(3), 383–387

Akita, H., Kimura, Z., Yusoff, M.Z.M., Nakashima, N., Hoshino, T.: Draft genome sequence of Burkholderia sp. strain CCA53, isolated from leaf soil, Genome Announc., 4(4), e00630-16

Akita, H., Kimura, Z., Yusoff, M.Z.M., Nakashima, N., Hoshino, T.: Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential, SpringerPlus, 5, 596

Fujii, T., Okuda, N., Inoue, H., Ishikawa, K., Hoshino, T.: Enhancing cellulase production by overexpression of xylanase regulator protein gene, xlnR, in Talaromyces cellulolyticus cellulase hyperproducing mutant strain, Biosci. Biotechnol. Biochem. 80(10), 2065-2068

Kimura, Z. I., Hoshino, T., and Murakami, K.: The status of the species Moorella thermoautotrophica (Wiegel et al., 1981). Request for an Opinion International Journal of Systematic and Evolutionary Microbiology, 66(8), 3249-3251

Kimura, Z. I., Kita, A., Iwasaki, Y., Nakashimada, Y., Hoshino, T., and Murakami, K.: Glycerol acts as alternative electron sink during syngas fermentation by thermophilic anaerobe Moorella thermoacetica, J. Biosci. Bioeng., 121(3), 268-273

Watanabe, M., Fukada, H., and Ishikawa, K.: Construction of Thermophilic Xylanase and Its Structural Analysis, Biochemistry 55(31), 4399-4409

Uechi, K., Kamachi, S., Akita, H., Mine, S., and Watanabe, M.: Crystal structure of an acetyl esterase complexed with acetate ion provides insights into the catalytic mechanism, Biochem. Biophys. Res. Commun. 477(3), 383-387

Inoue, H., Kitao, C., Yano, S., and Sawayama, S.: Production of β-xylosidase from Trichoderma asperellum KIF125 and its application in efficient hydrolysis of pretreated rice straw with fungal cellulase, World Journal of Microbiology and Biotechnology 32, 186

Matsushika, A., Negi, K., Suzuki, T., Goshima, T., and Hoshino, T.: Identification and characterization of a novel Issatchenkia orientalis GPI-anchored protein, IoGas1, required for resistance to low pH and salt stress, PLoS One 11(9), e0161888

Hasegawa, F., Inoue, H., Yano, S., Yokoyama, S., and Imou, K.: Evaluation of Cellulase Activity in Enzymatic Hydrolysis Residues for Efficient Enzyme Reuse, Journal of the Japan Institute of Energy 95, 930-936.

Okuda, N., Fujii, T., Inoue, H., Ishikawa, K., and Hoshino, T.: Enhancing cellulase production by overexpression of xylanase regulator protein gene, xlnR, in Talaromyces cellulolyticus cellulase hyperproducing mutant strain. Biosci. Biotechnol. Biochem. 80: 2065-2068.

Inoue, H., Fujimoto, S., and Sakaki, T.: Two-step hot-compressed water treatment of Douglas fir for efficient total sugar recovery by enzymatic hydrolysis. BioResources 11, 5124-5137.

Chang, Y.C., Fuzisawa, S., Reddy, M.V., Kobayashi, H., Yoshida, E., Yajima, Y., Hoshino, T., and Choi, D.B.: Degradation of toxic compounds at low and medium temperature conditions using isolated fungus, Clean-Soil Air Water 44(8), 992-1000

Singh, S.M., Tsuji, M., Gawas-Sakhalker, P., Loonen, M.J.J.E., and Hoshino, T.: Bird feather fungi from Svalbard Arctic, Polar Biol. 39(3), 523-532

Tsuji, M., Kudoh, S., and Hoshino, T.: Ethanol productivity of cryophilic basidiomycetous yeast Mrakia spp. correlates with ethanol tolerance, Mycoscience 57, 42−50

2015年

Akita, H., Watanabe, M., Suzuki, T., Nakashima, N., and Hoshino, T.: Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1, FEMS Microbiol. Lett., 362(16), fnv116

Akita, H., Seto, T., Ohshima, T., and Sakuraba, H.: Structural insight into the thermostable NADP+-dependent meso-diaminopimelate dehydrogenase from Ureibacillus thermosphaericus, Acta Crystallogr. D, D71(5), 1136–1146

Akita, H., Watanabe, M., Suzuki, T., Nakashima, N., and Hoshino, T.: Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase, AMB Express, 5, 17

Akita, H., Nakashima, N., and Hoshino, T.: Bacterial production of isobutanol without expensive reagents, Appl. Microbiol. Biotechnol., 99(2) 991–999

Fujii, T., Inoue, H., and Ishikawa, K.: Decreased cellulase and xylanase production in the fungus Talaromyces cellulolyticus by disruption of tacA and tctA genes, encoding putative zinc finger transcriptional factor, Appl. Biochem. Biotechnol. 175, 3218-3229

Fujii, T., Koike, K., Yano, S., Sawayama, S., and Inoue, H.: Draft Genome Sequence of Talaromyces cellulolyticus Y-94, a Source of Lignocellulosic Biomass-Degrading Enzyme, Genome Announc.. 3(1)

Inoue, H., Kishishita, S., Kumagai, A., Kataoka, M., Fujii, T., and Ishikawa, K.: Contribution of a family 1 carbohydrate-binding module in thermostable glycoside hydrolase 10 xylanase from Talaromyces cellulolyticus toward synergistic enzymatic hydrolysis of lignocellulose, Biotechnol. Biofuels. 8(77)

Kishishita, S., Fujii, T., and Ishikawa, K.: Heterologous expression of hyperthermophilic cellulases of archaea Pyrococcus sp. by fungus Talaromyces cellulolyticus, J. Ind. Microbiol. Biotechnol. 42, 137-141

Watanabe, M., Yoshida, E., Fukada, H., Inoue, H., Tokura, M., and Ishikawa, K.: Characterization of a feruloyl esterase B from Talaromyces cellulolyticus, Biosci. Biotechnol. Biochem. 79(11), 1845-1851

Watanabe, M., Fukada, H., Inoue, H., and Ishikawa, K.: Crystal structure of an acetylesterase from Talaromyces cellulolyticus and the importance of a disulfide bond near the active site, FEBS Lett. 589(11), 1200-1206,

Hoshino, T., Tsuji, M., Yajima, Y., Yoshimune, K., and Matsushika, A.: Improvement of saccharification and fermentation by removal of endogenous chemicals from pretreated lignocellulosic biomass (1). Effect of ion-exchange resin treatment, Ferment. Technol. 4, 114

Matsushika, A. and Hoshino, T.: Increased ethanol production by deletion of HAP4 in recombinant xylose-assimilating Saccharomyces cerevisiae, J. Ind. Microbiol. Biotechnol. 42(12), 1623-1631

de Sales, B. B., Scheid, B., Gonçalvesa, D. L., Knychala, M. M., Matsushika, A., Bon, E. P., and Stambuk, B. U.: Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing D-xylose fermentation by recombinant Saccharomyces cerevisiae, Biotechnol. Lett. 37(10), 1973-1982

Ishikawa, K., Kataoka, M., Yanamoto, T., Nakabayashi, M., Watanabe, M., Ishihara, S., and Yamaguchi, S.: Crystal structure of β-galactosidase from Bacillus circulans ATCC 31382 (BgaD) and the construction of the thermophilic mutants, FEBS Journal 282, 2540-52

Inoue, H., Yano, S., and Sawayama, S.: Effect of β-mannanase and β-mannosidase supplementation on the total hydrolysis of softwood polysaccharides by the Talaromyces cellulolyticus cellulase system, Appl. Biochem. Biotechnol. 176, 1673-1686

Paredes, R.S., Barros, R.R.O., Inoue, H., Yano, S., and Bon, E.P.S.: Production of xylanase, α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase by Aspergillus awamori using the liquid stream from hot-compressed water treatment of sugarcane bagasse, Biomass Convers. Biorefin. 5, 299-307

Tsuji, M., Kudoh, S., and Hoshino, T.: Draft genome sequence of cryophilic basidiomycetous yeast Mrakia blollopis SK-4 isolated from an algal mat of Naga-ike lake in Skarvsnes ice-free area, East Antarctica, Genome Announc. 3, e014554-14

Reddy, M.V., Yajima, Y., Mawatari, Y., Hoshino, T., and Chang, Y.C.: Degradation and conversion of toxic compounds into useful bioplactics by Cupriavidus sp. CY 1: Relative expression of PhaC gene under phenol and nitrogen stress, Green Chem. 192, 4560−4562

Ikeda, S., Hoshino, T., Matsumoto, N., and Kondo, N.: Taxonomic reappraisal of Typhula variabilis, Typhula laschii, Typhula intermedia, and Typhula japonica, Mycoscience 56, 549−559

Reddy, M.V., Mawatari, Y., Yajima, Y., Seki, C., Hoshino, T., and Chang, Y.C.: Poly-β-hydroxybutyrate (PHB) production from alkylphenols, mono and poly-aromatic hydrocarbons using Bacillus sp. CYR1: A new strategy for wealth from waste, Bioresour. Technol. 192, 711−717

Tsuji, M., Yokota, Y., Kudoh, S., and Hoshino, T.: Comparative analysis of milk fat decomposition activity by Mrakia spp. Isolated from Skarvsnes ice-free area, East Antarctica, Cryobiology 70, 293−296

Murakami, R., Yajima, Y., Kida, K., Tokura, K., Tojo, M., and Hoshino, T.: Surviving freezing in plant tissues by oomycetous snow molds, Cryobiology 70, 208−210

Gafforov, Y., and Hoshino, T.: Remarks on Typhula sp. in Uzbekistan, Mycoscience 56, 109−113

2014年

Nakashima, N., Akita, H., and Hoshino, T.: Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application for production of 2,3-butanediol and acetoin, Metab. Eng., 25, 204–214

Akita, H., Imaizumi, Y., Suzuki, H., Doi, K., and Ohshima, T.: Spectrophotometric assay of D-isoleucine using an artificially created D-amino acid dehydrogenase, Biotechnol. Lett., 36(11), 2245–2248

Fujii, T., Inoue, H., and Ishikawa, K.: Characterization of the xylanase regulator protein gene, xlnR, in Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus), Biosci. Biotechnol. Biochem. 78, 1564-1567

Fujii, T., Hoshino, T., Inoue, H., and Yano, S.: Taxonomic revision of the cellulose-degrading fungus Acremonium cellulolyticus nomen nudum to Talaromyces (Penicillium) based on phylogenetic analysis, FEMS Microbiol. Lett. 351(1), 32-42

Fujii, T., Murakami, K., Endo, T., Fujimoto, S., Minowa, T., Matsushika, A., Yano, S., and Sawayama, S.: Bench Scale Bioethanol Production from Eucalyptus by High Solid Saccharification and Glucose/Xylose Fermentation Method, Bioprocess Biosyst. Eng. 37, 749-754

Hayata, K., Asada, S., Fujii, T., Inoue, H., Ishikawa, K., and Sawayama, S.: Gene targeting by RNAi-mediated knockdown of potent DNA ligase IV homologue in the cellulase-producing fungus Talaromyces cellulolyticus, Appl. Biochem. Biotechnol. 174, 1697-1704

Watanabe, M., Inoue, H., Inoue, B., Yoshimi, M., Fujii, T., and Ishikawa, K.: Xylanase (GH11) from Acremonium cellulolyticus: homologous expression and characterization, AMB Express. 4(27)

Asada, S., Watanabe, S., Fujii, T., Inoue, H., Ishikawa, K., and Sawayama, S.: RNAi knockdown of potent sugar sensor in cellulase-producing fungus Acremonium cellulolyticus, Appl. Biochem. Biotechnol. 172, 3009-3015

Tsukahara, K., Kita, A., Nakashimada, Y., Hoshino, T., and Murakami, K.: Genome-guided analysis of transformation efficiency and carbon dioxide assimilation by Moorella thermoacetica Y72, Gene, 535(2), 150-155

Watanabe, M., and Ishikawa, K.: Crystallization and preliminary X-ray crystallographic analysis of a putative acetylxylan esterase from Talaromyces cellulolyticus, Acta Crystallogr. F Struct. Biol. Commun. 70(12), 1668-1670

Watanabe, M., and Ishikawa, K.: Crystallization and preliminary X-ray crystallographic analysis of a putative feruloyl esterase from Talaromyces cellulolyticus, Acta Crystallogr. F Struct. Biol. Commun. 70(12), 1664-1667

Mine, S., Kado, Y., Watanabe, M., Fukuda, Y., Abe, Y., Ueda, T., Kawarabayasi, Y., Inoue, T., and Ishikawa, K.: The structure of hyperthermophilic β-N-acetylglucosaminidase reveals a novel dimer architecture associated with the active site, FEBS J. 281(22), 5092-5103

Nakabayashi, M., Kataoka, M., Watanabe, M., and Ishikawa, K.: Monomer structure of a hyperthermophilic β-glucosidase mutant forming a dodecameric structure in the crystal form, Acta Crystallogr. F Struct. Biol. Commun. 70(7), 854-859

Watanabe, M., Fujiwara, M., and Ishikawa, K.: Encapsulation of Hyperthermophilic β-Glucosidase from Pyrococcus furiosus into Silica Microcapsules, Chem. Lett. 43(8), 1272-1274

Kishishita, S., Yoshimi, M., Fujii, T., Taylor, L.E., Decker, S.R., Ishikawa, K., and Inoue, H.: Cellulose-inducible xylanase Xyl10A from Acremonium cellulolyticus: purification, cloning and homologous expression, Protein Expres. Purif. 94, 40-45

Inoue, H., Hashimoto, S., Matsushika, A., Watanabe, S., and Sawayama, S.: Breeding of a xylose-fermenting hybrid strain by mating genetically engineered haploid strains derived from industrial Saccharomyces cerevisiae, J. Ind. Microbiol. Biotechnol. 41(12), 1773-1781

Matsushika, A., Morikawa, H., Goshima, T., and Hoshino, T.: Effect of fermentation conditions on the flocculation of recombinant Saccharomyces cerevisiae capable of co-fermenting glucose and xylose, Appl. Biochem. Biotechnol. 174(2), 623-631

Suzuki, T., Hoshino, T., and Matsushika, A.: Draft genome sequence of Kluyveromyces marxianus strain DMB1, isolated from sugarcane bagasse hydrolysate, Genome Announc. 2(4), e00733-14

Gonçalvesa, D. L., Matsushika, A., de Sales, B. B., Goshima, T., Bon, E. P., and Stambuk, B. U.: Xylose and xylose/glucose co-fermentation by recombinant Saccharomyces cerevisiae strains expressing individual hexose transporters, Enzyme Microb. Technol. 63, 13-20

Matsushika, A., Goshima, T., and Hoshino, T.: Transcription analysis of recombinant industrial and laboratory Saccharomyces cerevisiae strains reveals the molecular basis for fermentation of glucose and xylose, Microb. Cell Fact. 13, 16

Yu, G., Yano, S., Inoue, H., Inoue, S., Wang, J., and Endo, T.: Structural insights into rice straw pretreated by hot-compressed water in relation to enzymatic hydrolysis, Appl. Biochem. Biotechnol. 174, 2278-2294

Inoue, H., Decker, S.R., Taylor, L.E. II., Yano, S., and Sawayama, S.: Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass, Biotechnol. Biofuels 7, 151

Kataoka, M., Akita, F., Maeno, Y., Inoue, B., Inoue, H., and Ishikawa, K.: Crystal structure of Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus) GH family 11 xylanase, Appl. Biochem. Biotechnol. 174, 1599-1612

Tsuji, M., Yokota, Y., Kudoh, S., and Hoshino, T.: Improvement of direct ethanol fermentation from woody biomasses by Antarctic basidiomycetous yeast Mrakia blollopis under a low temperature condition, Cryobiology 68, 303−305

Narihiro, T., Suzuki, A., Yoshimune, K., Hori, T., Hoshino, T., Yumoto, I., Yokota, A., Kimura, N., and Kamagata, Y.: The combination of functional metagenomics and oil-fed enrichment strategy revealed the phylogenetic diversity of lipolytic bacteria overlooked by cultivation-based method, Microbes Environ. 29, 154−161

Tsuji, M., Yokota, Y., Kudoh, S., and Hoshino, T.: Effects of nitrogen concentration and culturing temperature on lipase secretion and morphology of the Antarctic basidiomycetous yeast Mrakia blollopis, Int. J. Res. Eng. Sci. 2, 49−54

Xiao, N., Hanada, Y., Seki, H., Kondo, H., Tsuda, S., and Hoshino, T.: Annealing condition influences thermal hysteresis of fungal type ice-binding proteins, Cryobiology 68, 159−161

Singh, P., Singh, S.M., Tsuji, M., Prasad, G.S., and Hoshino, T.: Rhodotorula svalbardensis sp. nov., a novel yeast species isolated from cryoconite holes of Ny-Ålesund, Arctic, Cryobiology 68, 122−128


著書・総説・解説

2020年

Wada K and Suzuki H: Biotechnological platform for the moderate thermophiles Geobacillus species. Physiological and Biotechnological aspects of extremophiles, Elsevier in press

2019年

Champreda V, Mhuantong W, Lekakarn H, Bunterngsook B, Kanokratana P, Zhao XQ, Zhang F, Inoue H, Fujii T, Eurwilaichitr L: Designing cellulolytic enzyme systems for biorefinery: From nature to application. J Biosci Bioeng. 128(6), 637-654.

2018年

Akita H, Hayashi J, Sakuraba H and Ohshima T: Artificial thermostable D-amino acid dehydrogenase: creation and application. Front. Microbiol. 9, 1760

Fujii T, Inoue H, Yano S, Sawayama S: Strain improvement for industrial production of lignocellulolytic enzyme by Talaromyces cellulolyticus. In: Fang X., Qu Y. (eds) Fungal Cellulolytic Enzymes. Springer, Singapore, pp135-154

松鹿昭則:木質系バイオマスからの有用物質生産に向けた酵母の育種開発, 酵母菌・麹菌・乳酸菌の産業応用展開, シーエムシー出版, 1-15

2017年

老木紗予子, 中道優介, 丸山如江, 村田幸作, 橋本渉:病原細菌による宿主細胞外グリコサミノグリカンの断片化・輸送・分解・代謝に関わる分子機構, 生化学 86(6), 866–871


2016年

秋田紘長, 櫻庭春彦, 大島敏久:耐熱性人工NADP+依存性D-アミノ酸脱水素酵素: 創製と応用, ビタミン, 90(11), 544-554

秋田紘長:大腸菌のカタボライト抑制解除, 生物工学会誌, 94(5), 269

星野保:雪と植物病原菌, 低温と環境科学の事典(「低温と環境科学の事典」編集委員会), 朝倉書店 (ISBN978-4-254-16128-1)

星野保, 辻雅晴, 横田祐司, 工藤栄, 内海洋, 湯本勲:南極産酵母の環境適応機構の解明とその産業利用, 生物工学会誌 94, 329-331

2015年

秋田紘長, 櫻庭晴彦, 大島敏久:耐熱性D-アミノ酸脱水素酵素の創製とその利用, 生化学 87(5), 580-588

星野保:岩波科学ライブラリー245菌世界紀行―誰も知らないきのこを追って, 岩波書店 (ISBN 978-4-00-029645-8)

Tsuji, M., Yokota, Y., Shimohara, K., Kudoh, S., and Hoshino, T.: An Application of wastewater treatment in a cold environment and stable lipase production of Antarctic basidiomycetous yeast Mrakia blollopis, In Bioremediation of Wastewater: Factors and Treatment (Sanchez O ed) Apple Academic Press, Watertown, 259−276 (ISBN 978-1771881623)

2014年

Murakami, K., and Kimura, Z. I.: Syngas Fermentation for Production of Biofuels and Commodity Chemicals, Journal of the Japan Institute of Energy, 93(7), 594-600

星野保, 鈴木俊宏, 松鹿昭則:バイオマスリファイナリーへの酵母利用の現状と展望, 日本エネルギー学会誌 93(7), 573-579

Murakami, K., and Kimura, Z.: Syngas Fermentation for Production of Biofuels and Commodity Chemicals, Journal of the Japan Institute of Energy 93, 594-600

星野保, 鈴木俊宏, 松鹿昭則:バイオリファイナリーへの酵母利用の現状と展望, 日本エネルギー学会誌, 93, 573−579

Tkachenko, O.B., and Hoshino, T,: Cryophilic fungi and oomycetes and their peculiarities (in Russian), Mikologiya i Fitopathologiya 48, 215−219

Yoshida, E., Yamauchi, M., Babu, A.G., Hoshino, T., Chang, Y.C., and Kikuchi, S.: Characterization of 4-butylphenol-degrading microorganism isolated from the Antarctic soil, Joint Seminar on Environmental Science and Disaster Mitigation Research 2013 (JSED2014) 35−36

阿部淳一ピーター, 稲葉重樹, 折原貴道, 小林久奏, 繁森亜美, 早乙女梢, 種山裕一, 田中栄爾, 出川洋介, 保坂健太郎, 細矢剛, 星野保, 山田明義:菌類ワールドへの誘い, 日本菌学会

星野保:広辞苑を3倍楽しむ<70>残雪, 科学, 84, 504


依頼講演

2019年

Inoue H: Bio-conversion technology of chemical production from biomass, IBD2019, Bangkok, Thailand

2018年

松鹿昭則:バイオエタノール, シンポジウム「講習会:基礎からわかるバイオ燃料」, 広島

2017年

Akita H: Creation and application of a thermostable artificial NADP+-dependent D-amino acid dehydrogenase, The 3rd International Conference of D-Amino Acid Research, Varese, Italy

Nakashima N, Nakamura Y, Kino Y, Akita H, Hoshino T: Novel genetic tools for manipulating Escherichia coli, 2017 Annual Meeting & International Symposium, Busan, Korea

渡邊真宏:酵素タンパク質の結晶構造解析から高機能化まで, GIC平成29年度第54回研修セミナー, 仙台

松鹿昭則:バイオマス利活用に向けたバイオ変換技術論, グリーンケミストリー特別講演会, 広島

松鹿昭則:バイオマスからの有用物質発酵生産に向けた酵母の育種開発, 第3回関西バイオ医療研究会, 大阪

Inoue, H.: Development of fungal cellulolytic enzyme system suitable for hydrolysis of agricultural residue, AGBIO2017, Bangkok, Thailand


2016年

渡邊真宏:精密構造解析による酵素の高機能化, JACI技術セミナー, 東京

2015年

Oshima, T., Akita, H., Doi, K., Sakuraba, H.: Thermostable NADP-dependent D-amino acid dehydrogenase created by site-directedmutation: characteristic sandaplication, 18th Japanese-German Work shop Enzyme Technology, Kyoto, Japan

渡邊真宏, 蒲池沙織, 秋田紘長, 上地敬子, 峯昇平:蛋白研ビームラインを利用したAIST-ISCの研究成果について, 大阪大学蛋白質研究所セミナー, 大阪

2014年

Fujii, T., Inoue, H., Watanabe, M., Ishikawa K.: Genetic and Protein Engineering of Cellulase Producing Fungi Talaromyces cellulolyticus (Formerly Acremonium cellulolyticus), MIE BIOFORUM 2014,Mie, Japan

Hoshino, T.: Ecological strategies of cryophilic fungi to tolerate freezing stress, The 10th International Mycological Congress (IMC10), Bangkok, Thailand

星野保, 辻雅晴, 工藤栄, 横田祐司:南極産酵母の環境適応機構の解明とその産業利用, 第7回新産業酵母研究会講演会, 東京

星野保:凍りながら大きくなる!南極酵母がつくる霜柱, 第2回日本微生物学連盟フォーラム《微生物~知られざるミクロのエンジニア》, 東京


知的財産

2018年

庄智裕, 酒井伸介, 斉藤政宏, 中島田豊, 岩崎祐樹, 星野保, 村上克治, 塚原建一郎, 喜多晃久:モーレラ属細菌の酢酸非生産・乳酸生産株, 特許第6320697号 (日本), 2018/4/13

井上宏之, 石川一彦, 藤井達也:Modified xylanase, US10100295B2 (米国), 2018/10/16

松鹿昭則, 五島徹也, 星野保:耐酸耐塩性付与方法と耐酸耐塩性酵母を用いた有用物質生産, 特許第6343754号 (日本), 2018/6/1

井上宏之, 石川一彦, 藤井達也Acremonium cellulolyticus由来のβ-グルコシダーゼ及びその利用, 特許第6332584号 (日本), 2018/05/11

松鹿昭則, 五島徹也, 星野保:代謝変換酵母によるエタノール産生の改良方法, 特許第6316629号 (日本), 2018/4/6

2017年

石川一彦, 岸下誠一郎, 藤井達也:古細菌由来タンパク質の分泌生産システム, 特許第9809825号 (日本), 2017/11/07

藤井達也, 井上宏之, 矢野伸一, 石川一彦:crel遺伝子が機能しない糸状菌変異株, 特許第6103584号 (日本), 2017/3/10

村田幸作, 河井重幸, 中道優介, 内田浩二, 野々部昌継:ATP依存キナーゼにポリリン酸利用能を付与する方法, 特許第6261166号 (日本), 2017/12/22

2016年

石川一彦, 井上宏之:新規キシラナーゼ, 特許6056870号 (日本), 2016/12/16

酒井伸介, 高岡一栄, 中島田豊, 岩崎祐樹, 矢野伸一, 村上克治, 喜多 晃久:モーレラ属細菌の遺伝子組換え法, 特許第5963538号 (日本),2016/07/08

井上宏之, 星野保, 渡邊真宏, 十倉充範, 矢萩大貴, 深田寛朗:セルラーゼ, WO/2017/217453, 2016/12/21

村上克治, 喜多 晃久, 矢野伸一:新規高温性酢酸生産菌, 特許第5963260号 (日本), 2016/07/08

石川一彦, 渡邊真宏, 栗原宏征, 笠原拓也, 山田千晶, 山田勝成:エンドキシラナーゼ変異体、バイオマス分解用酵素組成物及び糖液の製造方法, WO2016072448 (WO), 2016/12/05

藤井達也, 井上宏之, 石川一彦, 奥田直之:転写因子変異株、特開2016-187319 (日本)

2015年

高旻天, 矢野伸一, 井上宏之, 坂西欣也, 中田龍彦, 千葉恭一, 山本淳一, 岡田正史:ジャガイモ原料の糖化方法及び液体燃料の製造方法, 特許第5843264号 (日本), 2015/1/27

酒井伸介, 高岡一栄, 中島田豊, 岩崎祐樹, 矢野伸一, 村上克治, 喜多晃久, 澤山茂樹:乳酸生産菌, 特許第5858463号 (日本), 2015

松鹿昭則, 井上宏之, 澤山茂樹:キシロース代謝改変による効果的なエタノール生産法, 特許第5850418号(日本), 2015/12/11

2014年

井上宏之, 石川一彦, 藤井達也Acremonium cellulolyticus(アクレモニウム・セルロリティカス)由来のβ-グルコシダーゼ及びその利用, 特開2014-217312


外部予算

2019年

井上宏之:糸状菌のポリエステル分解システムを利用したプラスチック分解処理技術の開発, 科研費 (基盤研究C), 2019-2021

秋田紘長:貧栄養耐性細菌を利用したD-アミノ酸発酵法の開発, 科研費 (若手), 2019-2021

中道優介:着せ替え可能なオンデマンド多機能ウイルス様ナノ粒子の開発, 科研費 (基盤C), 2019-2021

和田圭介:混合糖の取込能力の向上を指向した適応進化による高発酵性大腸菌株の創出, 科研費 (若手), 2019-2021

2018年

中島田豊, 青井義輝, 加藤節, 村上克治, 松鹿昭則, 和田圭介:再生可能エネルギーを活用した有用物質高生産微生物デザイン, JST未来社会創造事業, 2018-2022

渡邊真宏, 松鹿昭則, 中道優介, 他:革新的バイオ素材・高機能品等の開発を加速するバイオインフォマティクス基盤技術の開発, 戦略的イノベーション創造プログラム (スマートバイオ産業・農業基盤技術), 2018-2022

木村善一郎, 村上克治, 松鹿昭則:電気を食べる微生物でバイオものづくりの新地平を拓く, 科研費(基盤研究(B)), 2018-2020

2017年

敷中一洋, 松鹿昭則, 井上宏之, 渡邊真宏:高純度同時糖化リグニンベース機能素材の開発, JST-ALCA, 2017-2020

2016年

秋田紘長:非可食バイオマスの直接発酵を可能とする宿主微生物の開発, 科研費(若手B), 2016-2017

松鹿昭則:並行複発酵に最適なキシロース発酵性酵母創製に向けた基盤研究, 科研費(基盤研究(C)), 2016-2018

2015年

2014年

秋田紘長:生物多様性保全のためのパーム油産業によるグリーン経済の推進プロジェクト, SATREPS, 2014-2017

藤井達也:糸状菌アクレモニウム・セルロリティカスの糖化酵素遺伝子の発現制御機構の解析, 科研費(若手B), 2014-2017

渡邊真宏:非可食性植物由来オリゴ糖製造技術開発, NEDO再委託研究, 2014-2016(継続2016-2018)

石川一彦, 渡邊真宏:セルロースからのポリマー原料の革新的製造プロセス研究, JST-ALCA, 2014-2015


受賞

2018年

和田圭介:日本生物工学会論文賞

2017年

秋田紘長:第6回D-アミノ酸学会奨励賞

2016年

秋田紘長:第33回(2016年度)井上研究奨励賞

2015年

松鹿昭則:平成27年度職員表彰(農林水産技術会議事務局長賞)

2014年