本文へスキップ

バイオ変換グループは、バイオマス資源から機能性化学品を生産するための研究開発を行っています。

TEL. 082-420-8284

〒739-0046 広島県東広島市鏡山3-11-32

業績ACCOMPLISHMENT

2016年以前の業績はこちらになります

原著論文

2019年

Nakamichi Y, Fouquet T, Ito S, Matsushika A, Inoue H: Mode of action of GH30-7 reducing-end xylose-releasing exoxylanase A (Xyn30A) from the filamentous fungus Talaromyces cellulolyticus, Appl Environ Microbiol. 85, e00552-19.

Suzuki T, Hoshino T, Matsushika A: High-temperature ethanol production by a series of recombinant xylose-fermenting Kluyveromyces marxianus strains, Enzyme Microb Technol. in press

Mine S and Watanabe M: Structural Insights into the Molecular Evolution of the Archaeal Exo-β-D-glucosaminidase. Int J Mol Sci. in press

Matsuzawa T, Watanabe M, Kameda T, Kameyama A, and Yaoi K: Cooperation between β-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae. FEBS J, in press

Akita H, Matsushika A and Kimura ZI: Enterobacter oligotrophica sp. nov., a novel oligotroph isolated from leaf soil. MicrobiologyOpen, in press

Nakabayashi M, Kamachi S, Malle D, Yanamoto T, Kishishita S, Fujii T, Inoue H, and Ishikawa K: Construction of thermostable cellobiohydrolase I from the fungus Talaromyces cellulolyticus by protein engineering, Protein Eng Des Sel., in press

Nakamichi Y, Fouquet T, Ito S, Watanabe M, Matsushika A, and Inoue H: Structural and functional characterization of a bifunctional GH30-7 xylanase B from the filamentous fungus Talaromyces cellulolyticus, J Biol Chem. 294(11), 4065-4078

2018年

Fouquet T, Sato H, Nakamichi Y, Matsushika A, and Inoue H: Electrospray multistage mass spectrometry in the negative ion mode for the unambiguous molecular and structural characterization of acidic hydrolysates from 4‐O‐methylglucuronoxylan generated by endoxylanases, J. Mass. Spectrom. 54(3), 213-221

Matsuzawa T#, Watanabe M#, Nakamichi Y, Fujimoto Z, and Yaoi K: Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme, J. Struct. Biol. 205(1), 84-90

Yuzawa S, Mirsiaghi M, Jocic R, Fujii T, Masson F, Benites VT, Baidoo EEK, Sundstrom E, Tanjore D, Pray TR, George A, Davis RW, Gladden JM, Simmons BA, Katz L, and Keasling JD: Short-chain ketone production by engineered polyketide synthases in Streptomyces albus, Nat Commun. 9(1):4569

Nakamichi Y, Miyazaki N, Tsutsumi K, Higashiura A, Narita H, Murata K, Nakagawa A: An assembly intermediate structure of Rice Dwarf Virus reveals a hierarchical outer capsid shell assembly mechanism, Structure 27(3), 439-448

Akita H, Kimura ZI and Hoshino T: Pseudomonas humi sp. nov., isolated from leaf soil. Arch. Microbiol. 201(2), 245–251

Akita H, Hayashi J, Sakuraba H and Ohshima T: Artificial thermostable D-amino acid dehydrogenase: creation and application. Front. Microbiol. 9, 1760

Ahmad N, Zakaria MR, Mohd Yusoff MZ, Fujimoto S, Inoue H, Ariffin H, Hassan MA, and Shirai Y: Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production, Molecules 23(6), E1310

Bunterngsook B, Laothanachareon T, Chotirotsukon C, Inoue H, Fujii T, Hoshino T, Roongsawang N, Kuboon S, Kraithong W, Techanan W, Kraikul N, and Champreda V: Development of tailor-made synergistic cellulolytic enzyme system for saccharification of steam exploded sugarcane bagasse, J. Biosci. Bioeng. 125(4), 390–396

Matsuzawa T#, Watanabe M#, and Yaoi K: Crystal structure of Aspergillus oryzae isoprimeverose-producing enzyme: New insight into the recognition of disaccharide in glycoside hydrolase family 3 enzymes, submitted

Watanabe M#, Matsuzawa T#, and Yaoi K: Rational protein design for thermostabilization of glycoside hydrolases based on structural analysis, Appl Microbiol Biotechnol 102(20), 8677-8684

2017年

Sornlake W, Rattanaphanjak P, Champreda V, Eurwilaichitr L, Kittisenachai S, Roytrakul S, Fujii T, Inoue H: Characterization of cellulolytic enzyme system of Schizophyllum commune mutant and evaluation of its efficiency on biomass hydrolysis, Biosci. Biotechnol. Biochem. 81(7), 1289–1299

Bunterngsook B, Laothanachareon T, Natrchalayuth S, Lertphanich S, Fujii T, Inoue H, Youngthong C, Chantasingh D, Eurwilaichitr L, and Champreda V: Optimization of a minimal synergistic enzyme system for hydrolysis of raw cassava pulp, Rsc Adv. 7(76), 48444–48453

Akita H,Kimura Z,and Matsushika A: Complete genome sequence of Ureibacillus thermosphaericus A1,a thermophilic bacillus isolated from compost, Genome Announc., 5(38), e00910-17

Yusoff MZM,Akita H,Mohd AH,Fujimoto S,Yoshida M,Nakashima N,Hoshino T: Production of acetoin from hydrothermally pretreated oil mesocarp fiber using metabolically engineered Escherichia coli in a bioreactor system, Bioresour. Technol., 245(Pt A), 1040–1048

Kimura Z,Akita H: Comparison of excess sludge reduction effect of dichlorophenol isomers, J. Pollut. Ef.f Cont., 5, 3

Akita H, Kimura Z, Yusoff MZM, Nakashima N, Hoshino T: Identification and characterization of Burkholderia multivorans CCA53, BMC Res. Notes, 10(1), 249

Akita H, Nakashima N, and Hoshino T: Production of D-lactate with pyruvate producing strain. Biosci. Biotechnol. Biochem., 81(7), 1452–1455

Hayashi J, Seto T, Akita H, Watanabe M, Hoshino T, Yoneda K, Ohshima T, and Sakuraba H: Structure based engineering of an artificially generated NADP+-dependent D-amino acid dehydrogenase, Appl. Environ. Microbiol., 83(11), e00491-17

Yuzawa S, Bailey CB, Fujii T, Jocic R, Barajas JF, Benites VT, Baidoo EEK, Chen Y, Petzold CJ, Katz L, and Keasling JD: Heterologous Gene Expression of N-Terminally Truncated Variants of LipPks1 Suggests a Functionally Critical Structural Motif in the N-terminus of Modular Polyketide Synthase. ACS Chem Biol. 12(11), 2725-2729

Fujii T, Inoue H, Ishikawa K, Hoshino T: Deletion Analysis of GH7 Endoglucanase Gene (cel7B) Promoter Region in a Talaromyces cellulolyticus ligD-Disrupted Strain. Appl. Biochem. Biotechnol. 183(4), 1516-1525

Iwasaki Y, Kita A Yoshida K, Tajima T, Yano S, Shou T, Saito M, Kato J, Murakami K, and Nakashimada Y: Homolactic Acid Fermentation by the Genetically Engineered Thermophilic Homoacetogen Moorella thermoacetica ATCC 39073, Appl. Environ. Microbiol. 83(8), e00247-17

Rahayu F, Kawai Y, Iwasakia Y, Yoshida K, Kita A, Tajima T, Kato J, Murakami K, and Nakashimada Y: Thermophilic ethanol fermentation from lignocellulose hydrolysate by genetically engineered Moorella thermoacetica, Bioresource Technology

Matsuzawa T#, Watanabe M#, and Yaoi K: Improved thermostability of a metagenomic glucose-tolerant β-glycosidase based on its crystal structure, Appl. Microbiol. Biotechnol. 101(23-24), 8353-8363

Mine S#, Watanabe M#, Kamachi S, Abe Y, and Ueda T: The Structure of an Archaeal β-Glucosaminidase Provides Insight into Glycoside Hydrolase Evolution, J. Biol. Chem. 292(12), 4996-5006

Matsushika A, Suzuki T, Goshima T, and Hoshino T: Evaluation of Saccharomyces cerevisiae GAS1 with respect to its involvement in tolerance to low pH and salt stress, J. Biosci. Bioeng. 124(2), 164-170

Kobayashi Y, Sahara T, Suzuki T, Kamachi S, Matsushika A, Hoshino T, Ohgiya S, Kamagata Y, and Fujimori K: Genetic improvement of xylose metabolism by enhancing the expression of pentose phosphate pathway genes in Saccharomyces cerevisiae IR-2 for high-temperature ethanol production, J. Ind. Microbiol. Biotechnol. 44(6), 879-891


著書・総説・解説

2019年

2018年

Fujii T, Inoue H, Yano S, Sawayama S: Strain improvement for industrial production of lignocellulolytic enzyme by Talaromyces cellulolyticus. In: Fang X., Qu Y. (eds) Fungal Cellulolytic Enzymes. Springer, Singapore, pp135-154

松鹿昭則:木質系バイオマスからの有用物質生産に向けた酵母の育種開発, 酵母菌・麹菌・乳酸菌の産業応用展開, シーエムシー出版, 1-15

2017年

老木紗予子, 中道優介, 丸山如江, 村田幸作, 橋本渉:病原細菌による宿主細胞外グリコサミノグリカンの断片化・輸送・分解・代謝に関わる分子機構, 生化学 86(6), 866–871


依頼講演

2019年

Inoue H: Bio-conversion technology of chemical production from biomass, IBD2019, Bangkok, Thailand

2018年

松鹿昭則:バイオエタノール, シンポジウム「講習会:基礎からわかるバイオ燃料」, 広島

2017年

Akita H: Creation and application of a thermostable artificial NADP+-dependent D-amino acid dehydrogenase, The 3rd International Conference of D-Amino Acid Research, Varese, Italy

Nakashima N, Nakamura Y, Kino Y, Akita H, and Hoshino T: Novel genetic tools for manipulating Escherichia coli, 2017 Annual Meeting & International Symposium, Busan, Korea

渡邊真宏:酵素タンパク質の結晶構造解析から高機能化まで, GIC平成29年度第54回研修セミナー, 仙台

松鹿昭則:バイオマス利活用に向けたバイオ変換技術論, グリーンケミストリー特別講演会, 広島

松鹿昭則:バイオマスからの有用物質発酵生産に向けた酵母の育種開発, 第3回関西バイオ医療研究会, 大阪

Inoue, H.: Development of fungal cellulolytic enzyme system suitable for hydrolysis of agricultural residue, AGBIO2017, Bangkok, Thailand


知的財産

2019年

2018年

庄智裕, 酒井伸介, 斉藤政宏, 中島田豊, 岩崎祐樹, 星野保, 村上克治, 塚原建一郎, 喜多晃久:モーレラ属細菌の酢酸非生産・乳酸生産株, 特許第6320697号 (日本), 2018/4/13

井上宏之, 石川一彦, 藤井達也:Modified xylanase, US10100295B2 (米国), 2018/10/16

松鹿昭則, 五島徹也, 星野保:耐酸耐塩性付与方法と耐酸耐塩性酵母を用いた有用物質生産, 特許第6343754号 (日本), 2018/6/1

井上宏之, 石川一彦, 藤井達也Acremonium cellulolyticus由来のβ-グルコシダーゼ及びその利用, 特許第6332584号 (日本), 2018/05/11

松鹿昭則, 五島徹也, 星野保:代謝変換酵母によるエタノール産生の改良方法, 特許第6316629号 (日本), 2018/4/6

2017年

石川一彦, 岸下誠一郎, 藤井達也:古細菌由来タンパク質の分泌生産システム, 特許第9809825号 (日本), 2017/11/07

藤井達也, 井上宏之, 矢野伸一, 石川一彦:crel遺伝子が機能しない糸状菌変異株, 特許第6103584号 (日本), 2017/3/10

村田幸作, 河井重幸, 中道優介, 内田浩二, 野々部昌継:ATP依存キナーゼにポリリン酸利用能を付与する方法, 特許第6261166号 (日本), 2017/12/22

外部予算

2019年

井上宏之:糸状菌のポリエステル分解システムを利用したプラスチック分解処理技術の開発, 科研費 (基盤研究C), 2019-2021

秋田紘長:貧栄養耐性細菌を利用したD-アミノ酸発酵法の開発, 科研費 (若手), 2019-2021

中道優介:着せ替え可能なオンデマンド多機能ウイルス様ナノ粒子の開発, 科研費 (基盤C), 2019-2021

和田圭介:混合糖の取込能力の向上を指向した適応進化による高発酵性大腸菌株の創出, 科研費 (若手), 2019-2021

2018年

中島田豊, 青井義輝, 加藤節, 村上克治, 松鹿昭則, 和田圭介:再生可能エネルギーを活用した有用物質高生産微生物デザイン, JST未来社会創造事業, 2018-2022

渡邊真宏, 松鹿昭則, 中道優介, 他:革新的バイオ素材・高機能品等の開発を加速するバイオインフォマティクス基盤技術の開発, 戦略的イノベーション創造プログラム (スマートバイオ産業・農業基盤技術), 2018-2022

木村善一郎, 村上克治, 松鹿昭則:電気を食べる微生物でバイオものづくりの新地平を拓く, 科研費(基盤研究(B)), 2018-2020

2017年

敷中一洋, 松鹿昭則, 井上宏之, 渡邊真宏:高純度同時糖化リグニンベース機能素材の開発, JST-ALCA, 2017-2020

受賞

2019年

2018年

和田圭介:日本生物工学会論文賞

2017年

秋田紘長:第6回D-アミノ酸学会奨励賞


産業技術総合研究所
材料・化学領域
機能化学研究部門
バイオ変換グループ

〒739-0046
広島県東広島市鏡山3-11-32

TEL;082-420-8284
FAX;082-420-8291
メール;a-matsushika
末尾に@aist.go.jpをつけて下さい