AIST-INDIA 機能性資源連携研究室

研究課題

我々が独自にクローニングした2つのタンパク質、モータリンとCARFは、がん細胞で過剰に発現することから、抗がん剤の新たな標的として注目されています。現在、それらのタンパク質について、以下のような検証を進めています。(i)ストレス、老化及びがん生物学における役割の解明、(ii)ストレス及びがん診断への有用性、(iii)ストレス及びがん介入のための阻害剤としての利用

研究課題

微細加工技術を利用して生理学性の高い培養環境を創出し、高次の細胞・臓器機能を発現可能な生体模倣システム（Microphysiological systems、MPS）の開発と産業応用を目指します。

研究課題

天然資源等からの新規な機能性物質探索を行うために、バイオアッセイを中心に実験を進めています。培養細胞を用いて、生体リズムなどを評価するリアルタイムレポータアッセイを取り入れた新しい検出方法を開発しています。

研究課題

直径200nmのナノニードルなど独自のナノ材料技術を駆使して、生命の精密かつ柔軟な動作原理を分子レベルで理解する研究を推進します。具体的には、細胞に極めてダメージの小さいナノニードル技術を用いた生細胞内での分子計測と分子操作、転移に関与する新規がんマーカーの機械的機能の解析とこれを標的とした診断および治療技術の開発などを目指して、研究を行っています。

研究課題

遺伝子発現制御（エピゲノム）やシグナル伝達制御（糖鎖）に関する知見を基盤に、神経・精神疾患の創薬に役立つ疾患モデルの開発と、薬剤や天然成分に関する効能・毒性評価系の構築を行っています。

研究課題

臓器間相互作用などの生体現象が観測可能な生体模倣システムを構築し、機能性食品や医薬に有用な成分のスクリーニングを行います。また、ノンコーディングRNAの観点を含め、得られた成分の作用メカニズムの解明を進めています。

業績リスト

• Kumar, V; Sari, AN; Gupta, D; Ishida, Y; Terao, K; Kaul, SC; Vrati, S; Sundar, D; Wadhwa, R.
  Anti-COVID-19 Potential of Withaferin-A and Caffeic Acid Phenethyl Ester.
  CURR TOP MED CHEM. 2024 Jan 24. doi: 10.2174/0115680266280720231221100004
• Kumar, V; Meidinna, HN; Kaul, SC; Gupta, D; Ishida, Y; Terao, K; Vrati, S; Sundar, D; Wadhwa, R.
  Molecular insights to the anti-COVID-19 potential of α-, β- and γ-cyclodextrins.
  J BIOMOL STRUCT DYN. 2023 Dec 20:1-11. doi: 10.1080/07391102.2023.2294385
• Zhang, HY; Zhang, ZY; Kaul, SC; Wadhawa, R.
  Antistress activity of some phytochemicals: screening, identification, validation, and potential applications.
  ANNALS OF NUTRITION AND METABOLISM. 2023;79 (suppl 1) 1087-1087; AUG. doi: 10.1159/000530786
• Viswan, A; Yoshikawa, C; Yamagishi, A; Furuhata, Y; Kato, Y; Yamazaki, T; Nakamura, C.
  Efficient genome editing by controlled release of Cas9 ribonucleoprotein in plant cytosol using polymer-modified microneedle array.
  BIOCHEM BIOPHYS RES COMMUN. 2023 Oct 29;686:149179. doi: 10.1016/j.bbrc.2023.149179
• Miyazawa, K; Penedo, M; Furusho, H; Ichikawa, T; Alam, MS; Miyata, K; Nakamura, C; Fukuma, T.
  Nanoendoscopy-AFM for Visualizing Intracellular Nanostructures of Living Cells.
  MICROSC MICROANAL. 2023 Jul 22;29(Supplement_1):782. doi: 10.1093/micmic/ozad067.387
• Ichikawa, T; Alam, MS; Penedo, M; Matsumoto, K; Fujita, S; Miyazawa, K; Furusho, H; Miyata, K; Nakamura, C; Fukuma, T.
  Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes.
  STAR PROTOC. 2023 Jul 22;4(3):102468. doi: 10.1016/j.xpro.2023.102468
• Kumar, V; Dhanjal, JK; Sari, AN; Khurana, M; Kaul, SC; Wadhwa, R; Sundar, D.
  Effect of Withaferin-A, Withanone, and Caffeic Acid Phenethyl Ester on DNA Methyltransferases: Potential in Epigenetic Cancer Therapy.
  CURR TOP MED CHEM. 2023 Jul 26. doi: 10.2174/1568026623666230726105017
• Kalra, RS; Chaudhary, A; Omar, A; Li, X; Khurana, M; Kaul, SC, Wadhwa, R.
  Stress-induced changes in CARF expression serve as a quantitative predictive measure of cell proliferation fate.
  EXP CELL RES. 2023 Jun 3:113669. doi: 10.1016/j.yexcr.2023.113669
• Huifu, H; Shefrin, S; Yang, S; Zhang, Z; Kaul, SC; Sundar, D; Wadhwa, R.
  Cucurbitacin-B inhibits cancer cell migration by targeting mortalin and HDM2: computational and in vitro experimental evidence.
  J BIOMOL STRUCT DYN. 2023 May 2:1-10. doi: 10.1080/07391102.2023.2206914
• Zhang, H; Wang, J; Prakash, J; Zhang, Z; Kaul, SC; Wadhwa, R.
  Three-way Cell-based Screening of Antistress Compounds: Identification, Validation, and Relevance to Old-age Related Pathologies.
  J GERONTOL A BIOL SCI MED SCI. 2023 Apr 15:glad103. doi: 10.1093/gerona/glad103
• Sugiura, S; Yamahira, S; Tamura, M; Shin, K; Shibuta, M; Satoh, T; Matsuzawa, Y; Fujii, G; Yanagawa, F; Mutoh, M; Yanagisawa, M; Kato, R; Matsui, H.
  Automated cell isolation from photodegradable hydrogel based on fluorescence image analysis.
  BIOTECHNOL BIOENG. 2023 Mar 15. doi: 10.1002/bit.28375
• Kumar, V; Sari, AN; Meidinna, HN; Kaul, A; Basu, B; Ishida, Y; Terao, K; Kaul, SC; Vrati, S; Sundar, D; Wadhwa, R.
  Computational and experimental evidence of the anti-COVID-19 potential of honeybee propolis ingredients, caffeic acid phenethyl ester and artepillin c.
  PHYTOTHER RES. 2023 Feb 9. doi: 10.1002/ptr.7717
• Huang, T; Sato, Y; Kuramochi, A; Ohba, Y; Sano, M; Miyagishi, M; Tateno, H; Wadhwa, R; Kawasaki, K; Uchida, T; Ekdahl, KN; Nilsson, B; Chung, UI; Teramura, Y.
  Surface modulation of extracellular vesicles with cell-penetrating peptide-conjugated lipids for improvement of intracellular delivery to endothelial cells.
  REGEN THER. 2023 Jan 11;22:90-98. doi: 10.1016/j.reth.2022.12.007
• Yu, Y; Zhang, GX; Li, ZP; Wang, J; Liu, Y; Bhardwaj, R; Wadhwa, R; Nagao, Y; Shichiri, M; Gao, R.
  Designed fabrication of active tumor targeting covalent organic framework nanotherapeutics via a simple post-synthetic strategy.
  NANO RESEARCH. 03 January 2023. doi: 10.1007/s12274-022-5265-7
• Limjanthong, N; Tohbaru, Y; Okamoto, T; Okajima, R; Kusama, Y; Kojima, H; Fujimura, A; Miyazaki, T; Kanamori, T; Sugiura, S; Ohnuma, K.
  Gravity-driven microfluidic device placed on a slow-tilting table enables constant unidirectional perfusion culture of human induced pluripotent stem cells.
  J BIOSCI BIOENG. 2022 Dec 29:S1389-1723(22)00346-2. doi: 10.1016/j.jbiosc.2022.11.007
• Sugiura, S; Shin, K; Kanamori, T.
  Perfusion culture of endothelial cells under shear stress on microporous membrane in a pressure-driven microphysiological system.
  J BIOSCI BIOENG. 2022 Oct 14:S1389-1723(22)00258-4. doi: 10.1016/j.jbiosc.2022.09.005
• Shefrin, S; Sari, AN; Kumar, V; Zhang, H; Meidinna, HN; Kaul, SC; Wadhwa, R; Sundar, D.
  Comparative computational and experimental analyses of some natural small molecules to restore transcriptional activation function of p53 in cancer cells harbouring wild type and p53(Ser46) mutant.
  CURR RES STRUCT BIOL. 2022 Sep 13;4:320-331. doi: 10.1016/j.crstbi.2022.09.002
• Meidinna, HN; Shefrin, S; Sari, AN; Zhang, H; Dhanjal, JK; Kaul, SC; Sundar, D; Wadhwa, R.
  Identification of a new member of Mortaparib class of inhibitors that target mortalin and PARP1.
  FRONT CELL DEV BIOL. 2022 Sep 12;10:918970. doi: 10.3389/fcell.2022.918970
• Sugiura, S; Satoh, T; Shin, K; Onuki-Nagasaki, R; Kanamori, T.
  Perfusion culture of multi-layered HepG2 hepatocellular carcinoma cells in a pressure-driven microphysiological system.
  J BIOSCI BIOENG. 2022 Aug 11:S1389-1723(22)00189-X. doi: 10.1016/j.jbiosc.2022.07.001
• Tomita, T; Kawano, Y; Kassai, M; Onda, H; Nakajima, Y; Miyazaki, K.
  Hydroxy-β-sanshool isolated from Zanthoxylum piperitum (Japanese pepper) shortens the period of the circadian clock.
  FOOD FUNCT. 2022 Aug 12. doi: 10.1039/d2fo01036d
• Yamagishi, A; Mizusawa, M; Uchida, K; Iijima, M; Kuroda, S; Fukazawa, K; Ishihara, K; Nakamura, C.
  Mechanical detection of interactions between proteins related to intermediate filament and transcriptional regulation in living cells.
  BIOSENS BIOELECTRON. 2022 Aug 6;216:114603. doi: 10.1016/j.bios.2022.114603
• Yoon, AR; Wadhwa, R; Kaul, SC; Yun, CO.
  Why is Mortalin a Potential Therapeutic Target for Cancer.
  FRONT CELL DEV BIOL. 2022 Jun 29;10:914540. doi: 10.3389/fcell.2022.914540
• Viswan, A; Yamagishi, A; Hoshi, M; Furuhata, Y; Kato, Y; Makimoto, N; Takeshita, T; Kobayashi, T; Iwata, F; Kimura, M; Yoshizumi, T; Nakamura, C.
  Microneedle Array-Assisted, Direct Delivery of Genome-Editing Proteins Into Plant Tissue.
  FRONT PLANT SCI. 2022 Jun 24;13:878059. doi: 10.3389/fpls.2022.878059
• Nagasaki, A; Katoh, K; Hoshi, M; Doi, M; Nakamura, C; Uyeda, TQP.
  Characterization of phalloidin-negative nuclear actin filaments in U2OS cells expressing cytoplasmic actin-EGFP.
  GENES TO CELLS. 2022 Feb 23. doi: 10.1111/gtc.12930
• Radhakrishnan, N; Kaul, SC; Wadhwa, R; Sundar, D.
  Phosphatidylserine Exposed Lipid Bilayer Models for Understanding Cancer Cell Selectivity of Natural Compounds: A Molecular Dynamics Simulation Study.
  MEMBRANES 12 (1);doi: 10.3390/membranes12010064 JAN
• Malik, V; Radhakrishnan, N; Kaul, SC; Wadhwa, R; Sundar, D.
  Computational Identification of BCR-ABL Oncogenic Signaling as a Candidate Target of Withaferin A and Withanone.
  BIOMOLECULES 12 (2);doi: 10.3390/biom12020212 FEB
• Sari, AN; Dhanjal, JK; Elwakeel, A; Kumar, V; Meidinna, HN; Zhang, H; Ishida, Y; Terao, K; Sundar, D; Kaul, SC; Wadhwa, R.
  A Low Dose Combination of Withaferin A and Caffeic Acid Phenethyl Ester Possesses Anti-Metastatic Potential In Vitro: Molecular Targets and Mechanisms.
  CANCERS (Basel). 2022 Feb 3;14(3):787. doi: 10.3390/cancers14030787
• Penedo, M; Miyazawa, K; Okano, N; Furusho, H; Ichikawa, T; Alam, MS; Miyata, K; Nakamura, C; Fukuma, T.
  Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM.
  SCI ADV. 2021 Dec 24;7(52):eabj4990. doi: 10.1126/sciadv.abj4990. Epub 2021 Dec 22
• Malik, V; Kumar, V; Kaul, SC; Wadhwa, R; Sundar, D.
  Potential of Withaferin-A, Withanone and Caffeic Acid Phenethyl ester as ATP-competitive inhibitors of BRAF: A bioinformatics study.
  CURR RES STRUCT BIOL. 2021 Nov 18;3:301-311. doi: 10.1016/j.crstbi.2021.11.004
• Kaul, A; Kuthethur, R; Ishida, Y; Terao, K; Wadhwa, R; Kaul, SC.
  Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C.
  MOLECULES. 2021 Dec 23;27(1):80. doi: 10.3390/molecules27010080
• Hori, K; Yoshimoto, S; Yoshino, T; Zako, T; Hirao, G; Fujita, S; Nakamura, C; Yamagishi, A; Kamiya, N.
  Recent advances in research on biointerfaces: From cell surfaces to artificial interfaces.
  J BIOSCI BIOENG. 2022 Jan 5:S1389-1723(21)00331-5. doi: 10.1016/j.jbiosc.2021.12.004
• Radhakrishnan, N; Dhanjal, JK; Sari, AN; Ishida, Y; Terao, K; Kaul, SC; Sundar, D; Wadhwa, R.
  Caffeic acid phenethyl ester (CAPE) confers wild type p53 function in p53(Y220C) mutant: bioinformatics and experimental evidence.
  DISCOVER ONCOLOGY 12 (1);doi: 10.1007/s12672-021-00461-2 DEC 20
• Wang, J; Zhang, H; Kaul, A; Li, K; Priyandoko, D; Kaul, SC; Wadhwa, R.
  Effect of Ashwagandha Withanolides on Muscle Cell Differentiation.
  BIOMOLECULES. 2021 Oct 4;11(10):1454. doi: 10.3390/biom11101454
• Kumar, V; Sari, AN; Meidinna, HN; Dhanjal, JK; Subramani, C; Basu, B; Kaul, SC; Vrati, S; Sundar, D; Wadhwa, R.
  Computational and in vitro experimental analyses of the anti-COVID-19 potential of Mortaparib and MortaparibPlus.
  BIOSCI REP. 2021 Oct 29;41(10):BSR20212156. doi: 10.1042/BSR20212156
• Bhargava, P; Mahanta, D; Kaul, A; Ishida, Y; Terao, K; Wadhwa, R; Kaul, SC.
  Experimental Evidence for Therapeutic Potentials of Propolis.
  NUTRIENTS. 2021 Jul 24;13(8):2528. doi: 10.3390/nu13082528
• Kumar, A; Konar, A; Garg, S; Kaul, SC; Wadhwa, R.
  Experimental evidence and mechanism of action of some popular neuro-nutraceutical herbs.
  NEUROCHEMISTRY INTERNATIONAL 149; doi: 10.1016/j.neuint.2021.105124
• Kaul, Z; Cheung, CTY; Bhargava, P; Sari, AN ; Yu, Y; He, HF; Bid, H; Henson, JD; Groden, J; Reddel, RR; Kaul, SC; Wadhwa, R.
  Functional characterization of miR-708 microRNA in telomerase positive and negative human cancer cells.
  SCI REP. 2021 Aug 23;11(1):17052. doi: 10.1038/s41598-021-96096-y
• Elwakeel, A; Sari, AN; Dhanjal, JK; Meidinna, HN; Sundar, D; Kaul, SC; Wadhwa, R.
  Mutant p53(L194F) Harboring Luminal-A Breast Cancer Cells Are Refractory to Apoptosis and Cell Cycle Arrest in Response to Mortaparib(Plus), a Multimodal Small Molecule Inhibitor.
  CANCERS (Basel). 2021 Jun 18;13(12):3043. doi: 10.3390/cancers13123043
• Yamagishi, A; Ito, F; Nakamura, C.
  Study on Cancer Cell Invasiveness via Application of Mechanical Force to Induce Chloride Ion Efflux.
  ANAL CHEM. 2021 Jun 21. doi: 10.1021/acs.analchem.1c01589
• Tomita, T; Wadhwa, R; Kaul, SC; Kurita, R; Kojima, N; Onishi, Y.
  Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa.
  J NAT PROD. 2021 Jun 21. doi: 10.1021/acs.jnatprod.0c01276
• Dhanjal, JK; Kumar, V; Garg, S; Subramani, C; Agarwal, S; Wang, J; Zhang, H; Kaul, A; Kalra, RS; Kaul, SC; Vrati, S; Sundar, D; Wadhwa, R
  Molecular mechanism of anti-SARS-CoV2 activity of Ashwagandha-derived withanolides
  Int J Biol Macromol. 2021 Jun 9:S0141-8130(21)01215-0. doi: 10.1016/j.ijbiomac.2021.06.015
• Li, X; Wang, J; Zhang, H; Xiao, L; Lei, Z; Kaul, SC; Wadhwa, R; Zhang, Z.
  Low Dose of Fluoride in the Culture Medium of Cordyceps militaris Promotes Its Growth and Enhances Bioactives with Antioxidant and Anticancer Properties.
  J FUNGI (Basel). 2021 Apr 28;7(5):342. doi: 10.3390/jof7050342
• Penedo, M; Shirokawa, T; Alam, MS; Miyazawa, K; Ichikawa, T; Okano, N; Furusho, H; Nakamura, C; Fukuma, T.
  Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells.
  SCI REP. 2021 Apr 8;11(1):7756. doi: 10.1038/s41598-021-87319-3
• Kalra, RS; Kumar, V; Dhanjal, JK; Garg, S; Li, X; Kaul, SC; Sundar, D; Wadhwa, R.
  COVID19-inhibitory activity of withanolides involves targeting of the host cell surface receptor ACE2: insights from computational and biochemical assays.
  J BIOMOL STRUCT DYN. 2021 Apr 2:1-14. doi: 10.1080/07391102.2021.1902858
• Sari AN, Elwakeel A, Dhanjal JK,Kumar V, Sundar D, Kaul SC, Wadhwa R
  Identification and Characterization of Mortaparib(Plus)-A Novel Triazole Derivative That Targets Mortalin-p53 Interaction and Inhibits Cancer-Cell Proliferation by Wild-Type p53-Dependent and -Independent Mechanisms.
  CANCERS (Basel). 2021 Feb 17;13(4):835. doi: 10.3390/cancers13040835
• Malik V, Kumar V, Kaul SC, Wadhwa R, Sundar D
  Computational Insights into the Potential of Withaferin-A, Withanone and Caffeic Acid Phenethyl Ester for Treatment of Aberrant-EGFR Driven Lung Cancers.
  BIOMOLECULES. 2021 Jan 26;11(2):160. doi: 10.3390/biom11020160
• Maejima A, Ishibashi K, Kim H, Kumagai I, Asano R
  Evaluation of intercellular cross-linking abilities correlated with cytotoxicities of bispecific antibodies with domain rearrangements using AFM force-sensing.
  BIOSENS BIOELECTRON. 2021 Jan 27;178:113037. doi: 10.1016/j.bios.2021.113037
• Wadhwa R, Yadav NS, Katiyar SP, Yaguchi T, Lee C, Ahn H, Yun CO, Kaul SC, Sundar D
  Molecular dynamics simulations and experimental studies reveal differential permeability of withaferin-A and withanone across the model cell membrane.
  SCI REP. 2021 Jan 27;11(1):2352. doi: 10.1038/s41598-021-81729-z
• Landeira JM, Liu BB, Omura T, Akiba T, Tanaka Y
  Salinity effects on the first larval stage of the invasive crab Hemigrapsus takanoi: Survival and swimming patterns
  ESTUARINE COASTAL AND SHELF SCIENCE 245; (2020) doi: 10.1016/j.ecss.2020.106976
• Konar A, Kalra RS, Chaudhary A, Nayak A, Guruprasa KP, Satyamoorthy K, Ishida Y, Terao K, Kaul SC, Wadhwa R
  Identification of Caffeic Acid Phenethyl Ester (CAPE) as a Potent Neurodifferentiating Natural Compound That Improves Cognitive and Physiological Functions in Animal Models of Neurodegenerative Diseases
  Front Aging Neurosci. 2020 Nov 10;12:561925. doi: 10.3389/fnagi.2020.561925
• Nishihara R, Niwa K, Tomita T, Kurita R
  Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence
  Bioconjug Chem. 2020 Nov 25. doi: 10.1021/acs.bioconjchem.0c00536
• Kim H, Ishibashi K, Iijima M, Kuroda S, Nakamura C
  Influence of Nivolumab for Intercellular Adhesion Force between a T Cell and a Cancer Cell Evaluated by AFM Force Spectroscopy
  Sensors (Basel) 20(19):E5723. (2020)doi: 10.3390/s20195723
• Priyanka, Wadhwa R, Chaudhuri R, Nag TC, Seth P
  Novel role of mortalin in attenuating HIV-1 Tat-mediated astrogliosis
  J Neuroinflammation.;17(1):276. (2020) doi: 10.1186/s12974-020-01912-3
• Yu Y, Yang X, Reghu S, Kaul SC, Wadhwa R, Miyako E
  Photothermogenetic inhibition of cancer stemness by near-infrared-light-activatable nanocomplexes
  Nat Commun. 11:4117 (2020) doi: 10.1038/s41467-020-17768-3
• Wang J, Bhargava P, Yu Y, Sari AN, Zhang H, Ishii N, Yan K, Zhang Z, Ishida Y, Terao K, Kaul SC, Miyako E, Wadhwa R
  Novel Caffeic Acid Phenethyl Ester-Mortalin Antibody Nanoparticles Offer Enhanced Selective Cytotoxicity to Cancer Cells
  Cancers (Basel). 12(9):E2370. (2020) doi: 10.3390/cancers12092370
• Malik V, Garg S, Afzal S, Dhanjal JK, Yun CO, Kaul SC, Sundar D, Wadhwa R
  Bioinformatics and Molecular Insights to Anti-Metastasis Activity of Triethylene Glycol Derivatives
  Int J Mol Sci. 21(15):E5463. (2020) doi: 10.3390/ijms21155463
• Kalra RS, Tomar D, Meena AS, Kandimalla R
  SARS-CoV-2, ACE2, and Hydroxychloroquine: Cardiovascular Complications, Therapeutics, and Clinical Readouts in the Current Settings
  Pathogens. 9: 0 (2020) doi: 10.3390/pathogens9070546
• Kumar V, Dhanjal JK, Bhargava P, Kaul A, Wang J, Zhang H, Kaul SC, Wadhwa R, Sundar D
  Withanone and Withaferin-A are predicted to interact with transmembrane protease serine 2 (TMPRSS2) and block entry of SARS-CoV-2 into cells
  J. Biomolecular Structure 21: 0 (2020) doi: 10.1080/07391102.2020.1775704
• Asano K, Kim HJ, Sakaki K, Nakamura YM, Wang YM, Isobe S, Doi M, Fujita A, Maejima N, Machida A, Watanuki T, Westerwaal RJ, Schreuders H, Dam B
  Metallurgical Synthesis of Mg2FexSi1-x Hydride: Destabilization of Mg2FeH6 Nanostructured in Templated Mg2Si
  Inorganic Chemistry 59: 2758-2764 (2020) doi: 10.1021/acs.inorgchem.9b03117
• Kumar V, Dhanjal JK, Bhargava P, Kaul A, Wang J, Zhang H, Kaul SC, Wadhwa R, Sundar D.
  Withanone and withaferin-A Are Predicted to Interact with Transmembrane Protease Serine 2 (TMPRSS2) and Block Entry of SARS-CoV-2 Into Cells
  J Biomol Struct Dyn. 1-27 (2020) doi: 10.1080/07391102.2020.1775704
• Kumar V, Dhanjal JK, Kaul SC, Wadhwa R, Sundar D
  Withanone and caffeic acid phenethyl ester are predicted to interact with main protease (Mpro) of SARS-CoV-2 and inhibit its activity
  J Biomol Struct Dyn 1-13 (2020) doi: 10.1080/07391102.2020.1772108
• Kim H, Hoshi M, Iijima M, Kuroda S, Nakamura C
  Development of a universal method for the measurement of binding affinities of antibody drugs towards a living cell based on AFM force spectroscopy
  Anal Methods (2020) doi: 10.1039/d0ay00788a
• Sari AN, Bhargava P, Dhanjal JK, Putri JF, Radhakrishnan N, Shefrin S, Ishida Y, Terao K, Sundar D, Kaul SC, Wadhwa R
  Combination of Withaferin-A and CAPE Provides Superior Anticancer Potency: Bioinformatics and Experimental Evidence to Their Molecular Targets and Mechanism of Action
  Cancers 12(5): E1160 (2020). doi: 10.3390/cancers12051160
• Omar A, Kalra RS, Putri J, Elwakeel A, Kaul SC, Wadhwa R
  Soyasapogenol-A targets CARF and results in suppression of tumor growth and metastasis in p53 compromised cancer cells
  Sci Rep. 10: 6323 (2020) doi: 10.1038/s41598-020-62953-5
• Kalra RS, Chaudhary A, Omar A, Cheung CT, Garg S, Kaul SC, Wadhwa R
  Stress-induced changes in CARF expression determine cell fate to death, survival, or malignant transformation
  Cell Stress Chaperones 25(3): 481-494 (2020). doi: 10.1007/s12192-020-01088-y
• Kurinomaru T, Inagaki A, Hoshi M, Nakamura C, Yamazoe H
  Protein microswimmers capable of delivering cells for tissue engineering applications
  Materials Horizons 7 7: 877-884 (2020) doi: 10.1039/c9mh01799b
• Afzal S, Garg S, Adiga D, Ishida Y, Terao K, Kaul SC, Wadhwa R
  Anti-stress, Glial- and Neuro-differentiation Potential of Resveratrol: Characterization by Cellular, Biochemical and Imaging Assays
  Nutrients 12(3): 671 (2020). doi: 10.3390/nu12030671
• Kim H, Ishibashi K, Okada T, Nakamura C
  Adhesion strengths between cancer cells with different malignancies and endothelial cells measured using atomic force microscopy
  Jpn J Appl Phys 59: SDDE01 (2020) doi: 10.7567/1347-4065/ab51ad
• Garg S, Huifu H, Kumari A, Sundar D, Kaul SC, Wadhwa R
  Induction of Senescence in Cancer Cells by a Novel Combination of Cucurbitacin B and Withanone: Molecular Mechanism and Therapeutic Potential
  J Gerontol A Biol Sci Med Sci. 75(6):1031-1041 (2020). doi: 10.1093/gerona/glz077
• Putri JF, Bhargava P, Dhanjal JK, Yaguchi T, Sundar D, Kaul SC, Wadhwa R
  Mortaparib, a novel dual inhibitor of mortalin and PARP1, is a potential drug candidate for ovarian and cervical cancers
  J Exp Clin Cancer Res. 38(1): 499 (2019). doi: 10.1186/s13046-019-1500-9
• Tomita T, Mori T, Onishi Y
  beta-Arrestin 1 (ARRB1) serves as a molecular marker of the peripheral circadian rhythm
  Int J Oral Sci. 11: 32 (2019) doi: 10.1038/s41368-019-0065-y
• Chaudhary A, Kalra RS, Malik V, Katiyar SP, Sundar D, Kaul SC, Wadhwa R
  2, 3-Dihydro-3beta-methoxy Withaferin-A Lacks Anti-Metastasis Potency: Bioinformatics and Experimental Evidences
  Sci Rep. 9(1):17344 (2019). doi: 10.1038/s41598-019-53568-6
• Garg S, Wu C, Ohmiya Y, Kaul SC, Wadhwa R
  Express ELISA for detection of mortalin
  Biotechniques. 67(4):166-171 (2019). doi: 10.2144/btn-2018-0158
• Kim H, Ishibashi K, Okada T, Nakamura C
  Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro
  Micromachines 10: 738 (2019) doi: 10.3390/mi10110738
• Konar A, Gupta R, Shukla RK, Maloney B, Khanna VK, Wadhwa R, , Lahiri DK, Thakur MK
  M1 muscarinic receptor is a key target of neuroprotection, neuroregeneration and memory recovery by i-Extract from Withania somnifera
  Sci Rep. 9(1):13990 (2019). doi: 10.1038/s41598-019-48238-6
• Kim H, Ishibashi K, Matsuo K, Kira A, Okada T, Watanabe K, Inada, M, Nakamura C
  Quantitative Measurements of Intercellular Adhesion Strengths between Cancer Cells with Different Malignancies Using Atomic Force Microscopy
  Anal Chem 91: 10557-10563 (2019) doi: 10.1021/acs.analchem.9b01569
• Yu Y, Wang J, Kaul SC, Wadhwa R, Miyako E
  Folic Acid Receptor-Mediated Targeting Enhances the Cytotoxicity, Efficacy, and Selectivity of Withania somnifera Leaf Extract: In vitro and in vivo Evidence
  Front Oncol. 9: 602 (2019). doi: 10.3389/fonc.2019.00602
• Garg S, Afzal S, Elwakeel A, Sharma D, Radhakrishnan N, Dhanjal JK, Sundar D, Kaul SC, Wadhwa R
  Marine Carotenoid Fucoxanthin Possesses Anti-Metastasis Activity: Molecular Evidence
  Mar Drugs. 17(6): 338 (2019). doi: 10.3390/md17060338
• Wadhwa R, Li L, Singh R, Wang J, Gao R, Nigam N, Forestier S, Ando N, Kaul SC
  Modulation of Diacylglycerol-Induced Melanogenesis in Human Melanoma and Primary Melanocytes: Role of Stress Chaperone Mortalin
  Evid Based Complement Alternat Med. 10: 800-805 (2019) doi: 10.1155/2019/9848969
• Yamagishi A, Susaki M, Takano Y, Mizusawa M, Mishima M, Iijima M, Kuroda S, Okada T, Nakamura C
  The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis
  INTERNATIONAL J. Biological Sciences 15: 1546-1556 (2019) doi: 10.7150/ijbs.33423
• Irie H, Ito K, Fujioka Y, Oguchi K, Fujioka A, Hashimoto A, , Ohsawa H, Tanaka K, Funabashi K, Araki H, Kawai Y, Shimamura T, , Wadhwa R, Ohkubo S, Matsuo K
  TAS0728, A Covalent-binding, HER2-selective Kinase Inhibitor Shows Potent Antitumor Activity in Preclinical Models
  Mol Cancer Ther. 18: 733-742 (2019) doi: 10.1158/1535-7163. MCT-18-1085
• Kalra RS, Bapat SA
  Proteomics to Predict Loss of RXR-gamma During Progression of Epithelial Ovarian Cancer
  Methods Mol Biol. 19: 0 (2019) doi: 10.1007/978-1-4939-9585-1_1
• Yamagishi A, Matsumoto D, Kato Y, Honda Y, Morikawa M, Iwata F, , Kobayashi T, Nakamura C
  Direct Delivery of Cas9-sgRNA Ribonucleoproteins into Cells Using a Nanoneedle Array
  Applied Sciences-Basel 9: 10 (2019) doi: 10.3390/app9050965
• Afzal S, Garg S, Ishida Y, Terao K, Kaul SC, Wadhwa R
  Rat Glioma Cell-Based Functional Characterization of Anti-Stress and Protein Deaggregation Activities in the Marine Carotenoids, Astaxanthin and Fucoxanthin
  Mar Drugs. 17(3):189 (2019). doi: 10.3390/md17030189
• Furuhata Y, Sakai A, Murakami T, Morikawa M,Nakamura C,Yoshizumi, T, Fujikura U, Nishida K, Kato Y
  A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall
  Scientific Reports 509: 943-948 (2019) doi: 10.1038/s41598-018-38119-9
• Odaka M, Kim H, Nakamura Y, Hattori A, Matsuura K, Iwamura M, Miyagi Y, Yasuda K
  Size Distribution Analysis with On-Chip Multi-Imaging Cell Sorter for Unlabeled Identification of Circulating Tumor Cells in Blood
  Micromachines10:154 (2019) doi: 10.3390/mi10020154
• Sundar D, Yu Y, Katiyar SP, Putri JF, Dhanjal JK, Wang J, Sari AN, Kolettas E, Kaul SC, Wadhwa R
  Wild type p53 function in p53(Y220C) mutant harboring cells by treatment with Ashwagandha derived anticancer withanolides: bioinformatics and experimental evidence
  J Exp Clin Cancer Res. 38(1): 103 (2019). doi: 10.1186/s13046-019-1099-x
• Bhargava P, Malik V, Liu Y, Ryu J, Kaul SC, Sundar D, Wadhwa R
  Molecular Insights into Withaferin-A-Induced Senescence: Bioinformatics and Experimental Evidence to the Role of NFkappaB and CARF
  J Gerontol A Biol Sci Med Sci. 74: 183-191 (2019) doi: 10.1093/gerona/gly107
• Garg S, Kaul SC, Wadhwa R
  Anti-Stress and Glial Differentiation Effects of a Novel Combination of Cucurbitacin B and Withanone (CucWi-N): Experimental Evidence
  Ann Neurosci. 25: 201-209 (2018) doi: 10.1159/000490693
• Ishida Y, Gao R, Shah N, Bhargava P, Furune T, Kaul SC, Terao K, Wadhwa R
  Anticancer Activity in Honeybee Propolis: Functional Insights to the Role of Caffeic Acid Phenethyl Ester and Its Complex With gamma-Cyclodextrin
  Integr Cancer Ther. 17: 867-873 (2018) doi: 10.1177/1534735417753545
• Bhargava P, Kumari A, Putri JF, Ishida Y, Terao K, Kaul SC, Sundar D, Wadhwa R
  Caffeic acid phenethyl ester (CAPE) possesses pro-hypoxia and anti-stress activities: bioinformatics and experimental evidences
  Cell Stress Chaperones. 23: 1055-1068 (2018) doi: 10.1007/s12192-018-0915-0
• Hasako S, Terasaka M, Abe N, Uno T, Ohsawa H, Hashimoto A, Fujita R, Tanaka K, Okayama T, Wadhwa R, Miyadera K, Aoyagi Y, Yonekura K, Matsuo K
  TAS6417, A Novel EGFR Inhibitor Targeting Exon 20 Insertion Mutations
  Mol Cancer Ther. 17: 1648-1658 (2018) doi: 10.1158/1535-7163.MCT-17-1206
• Garg S, Huifu H, Kaul SC, Wadhwa R
  Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence
  BMC Res Notes. 11: 403 (2018) doi: 10.1186/s13104-018-3512-5
• Nagasaki A, Kato Y, Meguro K, Yamagishi A, Nakamura C, Uyeda TQP
  A genome editing vector that enables easy selection and identification of knockout cells
  Plasmid. 9: 3829 (2018) doi: 10.1016/j.plasmid.2018.08.005
• Yu Y, Nishikawa M, Liu M, Tei T, Kaul SC, Wadhawa R, Zhang MF, Takahashi J, Miyako E
  Self-assembled nanodiamond supraparticles for anticancer chemotherapy
  Nanoscale 10: 8969-8978 (2018) doi: 10.1039/c8nr00641e
• Kalra RS, Chaudhary A, Yoon AR, Bhargava P, Omar A, Garg S, Yun, CO, Kaul SC, Wadhwa R
  CARF enrichment promotes epithelial-mesenchymal transition via Wnt/beta-catenin signaling: its clinical relevance and potential as a therapeutic target
  Oncogenesis. 7(5): 39 (2018). doi: 10.1038/s41389-018-0048-4
• Kaur A, Singh B, Ohri P, Wang J, Wadhwa R, Kaul SC, Pati PK, Kaur A
  Organic cultivation of Ashwagandha with improved biomass and high content of active Withanolides: Use of Vermicompost
  PLoS One. 13(4): e0194314 (2018). doi: 10.1371/journal.pone.0194314
• Nishio M, Yamagishi A, Tsukakoshi K, Kato Y, Nakamura C, Ikebukuro, K
  Selection and Characterization of DNA Aptamers Against FokI Nuclease Domain
  Zinc Finger Proteins 509: 943-948 (2018) doi: 10.1007/978-1-4939-8799-3_12
• Oh E, Garg S, Liu Y, Afzal S, Gao R, Yun CO, Kaul SC, Wadhwa R
  Identification and Functional Characterization of Anti-metastasis and Anti-angiogenic Activities of Triethylene Glycol Derivatives
  Front Oncol. 8: 552 (2018). doi: 10.3389/fonc.2018.00552
• Bhargava P, Grover A, Nigam N, Kaul A, Doi M, Ishida Y, Kakuta H, Kaul SC, Terao K, Wadhwa R
  Anticancer activity of the supercritical extract of Brazilian green propolis and its active component, artepillin C: Bioinformatics and experimental analyses of its mechanisms of action
  Int J Oncol. 52: 925-932 (2018) doi: 10.3892/ijo.2018.4249
• Garg S, Kaul SC, Wadhwa R
  Cucurbitacin B and cancer intervention: Chemistry, biology and mechanisms (Review
  Int J Oncol. 52: 19-37 (2018) doi: 10.3892/ijo.2017.4203.
• Li L, Gao R, Yu Y, Kaul Z, Wang J, Kalra RS, Zhang Z, Kaul SC, Wadhwa R
  Tumor suppressor activity of miR-451: Identification of CARF as a new target
  Sci Rep. 8: 375 (2018) doi: 10.1038/s41598-017-18559-5
• Kim H, Ishibashi K, Matsuo K, Kira A, Onomura Y, Okada T, Nakamura C
  Adhesion strength of a living cell to various substrates measured using a cup-attached AFM chip
  Jpn J Appl Phys 57: 03EB01 (2018) doi: 10.7567/JJAP.57.03EB01
• Ryu, S, Matsumoto Y, Matsumoto T, Ueno T, Silberberg YR, Nakamura C
  Improved efficiency of nanoneedle insertion by modification with a cell-puncturing protein
  Jpn J Appl Phys 57: 03EB02 (2018) doi.org/ 10.7567/JJAP.57.03EB02
• Kawamura R, Miyazaki M, Shimizu K, Matsumoto Y, Silberberg YR, Sathuluri RR, Iijima M, Ruroda S, Iwata F, Kobayashi T, Nakamura C
  A New Cell Separation Method Based on Antibody-Immobilized Nanoneedle Arrays for the Detection of Intracellular Markers
  Nano Lett. 17: 7117-7124 (2017) doi: 10.1021/acs.nanolett.7b03918
• Chaudhary A, Kalra RS, Huang C, Prakash J, Kaul SC, Wadhwa R
  2,3-Dihydro-3beta-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity
  J Nat Prod. 80: 2756-2760 (2017) doi: 10.1021/acs.jnatprod.7b00573
• Lee WJ, Ahn HM, Na Y, Wadhwa R, Hong J, Yun CO
  Mortalin deficiency suppresses fibrosis and induces apoptosis in keloid spheroids
  Sci Rep. 7(1):12957(2017) doi: 10.1038/s41598-017-13485-y
• Putri JF, Widodo N, Sakamoto K, Kaul SC, Wadhwa R
  Induction of senescence in cancer cells by 5-Aza-2-deoxycytidine: Bioinformatics and experimental insights to its targets
  Comput Biol Chem. 125: 168-174 (2017) doi: 10.1016/j.compbiolchem.2017.08.003
• Wadhwa R, Kalra RS, Kaul SC
  CARF is a multi-module regulator of cell proliferation and a molecular bridge between cellular senescence and carcinogenesis
  Mech Ageing Dev. 14: 3025-3035 (2017) doi: 10.1016/j.mad.2017.07.008
• Kim H, Yamagishi A, Imaizumi M, Onomura Y, Nagasaki A, Miyagi Y, Okada T, Nakamura C
  Quantitative measurements of intercellular adhesion between a macrophage and cancer cells using a cup-attached AFM chip
  Colloids Surf B Biointerfaces 37: 366-372 (2017) doi: 10.1016/j.colsurfb.2017.04.039
• Girault M, Kim H,, Arakawa H, Matsuura K, Odaka M, Hattori A, Terazono H, Yasuda K
  An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution
  Sci Rep 7: 40072 (2017) doi: 10.1038/srep40072
• Nishio M, Matsumoto D, Kato Y, Abe K, Lee J, Tsukakoshi K, Yamagishi A, Nakamura C,, Ikebukuro K
  DNA aptamers against FokI nuclease domain for genome editing applications
  Biosens. Bioelectron. 93: 26-31 (2017) doi: 10.1016/j.bios.2016.11.042
• Tomita T, Kurita R, Onishi Y
  Epigenetic regulation of the circadian clock: role of 5-aza-2-deoxycytidine
  Biosci Rep. 37: 0 (2017) doi: 10.1042/BSR20170053
• Nagasaki A, T Kijima S, Yumoto T, Imaizumi M, Yamagishi A, Kim H, Nakamura C, Q P Uyeda T
  The Position of the GFP Tag on Actin Affects the Filament Formation in Mammalian Cells
  Cell Struct Funct. 42: 131-140 (2017) doi: 10.1247/csf.17016
• Singh P, Guleri R, Angurala A, Kaur K, Kaur K, Kaul SC, Wadhwa R, Pati PK
  Addressing Challenges to Enhance the Bioactives of Withania somnifera through Organ, Tissue, and Cell Culture Based Approaches
  Biomed Res Int. 486: 539-544 (2017) doi: 10.1155/2017/3278494
• Yu Y, Katiyar SP, Sundar D, Kaul Z, Miyako E, Zhang Z, Kaul SC, Reddel RR, Wadhwa R
  Withaferin-A kills cancer cells with and without telomerase: chemical, computational and experimental evidences
  Cell Death Dis. 8(4): e2755 (2017). doi: 10.1038/cddis.2017.33
• Yun CO, Bhargava P, Na Y, Lee JS, Ryu J, Kaul SC, Wadhwa R.
  Relevance of Mortalin to Cancer Cell Stemness and Cancer Therapy
  Sci. Rep. (2017) 7: 42016. doi: 10.1038/srep42016