Cellular and Molecular Biotechnology Research Institute,
National Institute of Advanced Industrial Science (AIST)

Nanotechnology-Based Cell Engineering
(Nakamura-Kim-Hirano-Yamagishi Lab),
Tokyo University of Agriculture and Technology (TUAT)

JAPANESE

Publications

2025

  1. Fibrillin-1 G234D mutation in the hybrid1 domain causes tight skin associated with dysregulated elastogenesis and increased collagen cross-linking in mice
    Asm Sakhawat Hossain, Maria Thea Rane Dela Cruz Clarin, Kenichi Kimura, George Biggin, Yuki Taga, Koichiro Uto, Ayana Yamagishi, Eri Motoyama, Narenmandula, Kazunori Mizuno, Chikashi Nakamura, Keiichi Asano, Sumio Ohtsuki, Tomoyuki Nakamura, Sachiko Kanki, Clair Baldock, Erna Raja, Hiromi Yanagisawa
    Matrix Biology, 135, 24-38 (2025)

2023

  1. Efficient genome editing by controlled release of Cas9 ribonucleoprotein in plant cytosol using polymer-modified microneedle array
    Anchu Viswan, Chiaki Yoshikawa, Ayana Yamagishi, Yuichi Furuhata, Yoshio Kato, Tomohiko Yamazaki, Chikashi Nakamura
    Biochemical and Biophysical Research Communications, 686(17), 149179 (2023)
  2. Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes
    Takehiko Ichikawa, Mohammad Shahidul Alam, Marcos Penedo, Kyosuke Matsumoto, Sou Fujita, Keisuke Miyazawa, Hirotoshi Furusho, Kazuki Miyata, Chikashi Nakamura, Takeshi Fukuma
    STAR Protocols, 4(3), 102468 (2023)
  3. New Sensing Technologies: Atomic Force Microscopy
    Chikashi Nakamura and Hyonchol Kim
    Encyclopedia of Sensors and Biosensors, 1, 556-571 (2023)

2022

  1. Mechanical detection of interactions between proteins related to intermediate filament and transcriptional regulation in living cells
    Ayana Yamagishi, Mei Mizusawa, Koki Uchida, Masumi Iijima, Shun’ichi Kuroda, Kyoko Fukazawa, Kazuhiko Ishihara, Chikashi Nakamura
    Biosensors and Bioelectronics, 216, 114603 (2022)
  2. Microneedle array-assisted, direct delivery of genome-editing proteins into plant tissue
    Anchu Viswan, Ayana Yamagishi, Masamichi Hoshi, Yuichi Furuhata, Yoshio Kato, Natsumi Makimoto, Toshihiro Takeshita, Takeshi Kobayashi, Futoshi Iwata, Mitsuhiro Kimura, Takeshi Yoshizumi and Chikashi Nakamura
    Frontiers in Plant Science, 13, 878059 (2022)
  3. Characterization of phalloidin-negative nuclear actin filaments in U2OS cells expressing cytoplasmic actin-EGFP
    Akira Nagasaki, Kaoru Katoh, Masamichi Hoshi, Motomichi Doi, Chikashi Nakamura, Taro Q. P. Uyeda
    Genes to Cells, 27, 317-330 (2022)
  4. Recent advances in research on biointerfaces: From cell surfaces to artificial interfaces
    Katsutoshi Hori, Shogo Yoshimoto, Tomoko Yoshino, Tamotsu Zako, Gen Hirao, Satoshi, Fujita, Chikashi, Nakamura, Ayana Yamagishi, Noriho Kamiya
    Journal of Bioscience and Bioengineering, 133, 195-207 (2022)

2021

  1. Visualizing intra-cellular nanostructures of living cells by nanoendoscopy-AFM
    Marcos Penedo, Keisuke Miyazawa, Naoko Okano, Hirotoshi Furusho, Takehiko Ichikawa, Mohammad Shahidul Alam, Kazuki Miyata, Chikashi Nakamura, Takeshi Fukuma
    Science Advances, 7, eabj4990 (2021)
  2. Study on cancer cell invasiveness via application of mechanical force to induce chloride ion efflux
    Ayana Yamagishi, Fumie Ito, Chikashi Nakamura
    Analytical Chemistry, 93, 9032-9035 (2021)
  3. Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells
    Marcos Penedo, Tetsuya Shirokawa, Mohammad Shahidul Alam, Keisuke Miyazawa, Takehiko Ichikawa, Naoko Okano, Hirotoshi Furusho, Chikashi Nakamura, Takeshi Fukuma
    Scientific Reports, 11:7756 (2021)

2020

  1. Influence of Nivolumab for Intercellular Adhesion Force between a T Cell and a Cancer Cell Evaluated by AFM Force Spectroscopy
    Hyonchol Kim, Kenta Ishibashi, Masumi Iijima, Shun'ichi Kuroda, Chikashi Nakamura
    Sensors (Basel) 20(19), E5723, (2020)
  2. Development of a universal method for the measurement of binding affinities of antibody drugs towards a living cell based on AFM force spectroscopy
    Hyonchol Kim, Masamichi Hoshi, Masumi Iijima, Shun'ichi Kuroda and Chikashi Nakamura
    Analytical Methods, 12, 2922–2927 (2020)
  3. Protein microswimmers capable of delivering cells for tissue engineering applications
    Takaaki Kurinomaru, Akiko Inagaki, Masamichi Hoshi, Chikashi Nakamura and Hironori Yamazoe
    Materials Horizons, 7, 877-884 (2020)
  4. Adhesion strengths between cancer cells with different malignancies and endothelial cells measured using atomic force microscopy
    Hyonchol Kim, Kenta Ishibashi, Tomoko Okada, Chikashi Nakamura
    Japanese Journal of Applied Physics, 59, SDDE01 (2020)

2019

  1. Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro
    Hyonchol Kim, Kenta Ishibashi, Tomoko Okada, Chikashi Nakamura
    Micromachines 10 (11), 738, (2019)
  2. Quantitative measurements of intercellular adhesion strengths between cancer cells having different malignancies using atomic force microscopy
    Hyonchol Kim, Kenta Ishibashi, Kosuke Matsuo, Atsushi Kira, Tomoko Okada, Kenta Watanabe, Masaki Inada, Chikashi Nakamura
    Analytical Chemistry, 91(16), 10557-10563 (2019)
  3. The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis
    Ayana Yamagishi, Moe Susaki, Yuta Takano, Mei Mizusawa, Mari Mishima, Masumi Iijima, Shun'ichi Kuroda, Tomoko Okada, Chikashi Nakamura*
    International Journal of Biological Sciences, 15(7), 1546-1556 (2019)
  4. Direct delivery of Cas9-sgRNA ribonucleoproteins into cells using a nanoneedle array
    Ayana Yamagishi, Daisuke Matsumoto, Yoshio Kato, Yuki Honda, Mone Morikawa, Futoshi Iwata, Takeshi Kobayashi, Chikashi Nakamura*
    Applied Sciences-Basel, 9(5), 965 (2019)
  5. A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall
    Yuichi Furuhata, Ayako Sakai, Tomi Murakami, Mone Morikawa, Chikashi Nakamura, Takeshi Yoshizumi, Ushio Fujikura, Keiji Nishida, Yoshio Kato
    Scientific Reports, 9, 2163 (2019)

2018

  1. A genome editing vector that enables easy selection and identification of knockout cells
    Akira Nagasaki, Yoshio Kato, Keiichi Meguro, Ayana Yamagishi, Chikashi Nakamura, Taro Q.P. Uyeda Plasmid, 98, 37-44 (2018)
  2. Improved efficiency of nanoneedle insertion by modification with a cell-puncturing protein Seunghwan Ryu, Yuta Matsumoto, Takahiro Matsumoto, Takafumi Ueno, Yaron R. Silberberg and Chikashi Nakamura*
    Japanese Journal of Applied Physics, 57(3S2), 03EB02 (2018)
  3. Adhesion strength of a living cell to various substrates measured using a cup-attached AFM chip Hyonchol Kim, Kenta Ishibashi, Kosuke Matsuo, Atsushi Kira, Yui Onomura, Tomoko Okada and Chikashi Nakamura
    Japanese Journal of Applied Physics, 57(3S2), 03EB01 (2018)

2017

  1. A new cell separation method based on antibody-immobilized nanoneedle arrays for the detection of intracellular markers
    Ryuzo Kawamura, Minami Miyazaki, Keita Shimizu, Yuta Matsumoto, Yaron R. Silberberg,　Ramachandra Rao Sathuluri, Masumi Iijima, Shun’ichi Kuroda, Futoshi Iwata, Takeshi Kobayashi and Chikashi Nakamura*
    Nano Letters, 17(11), 7117-7124 (2017)
  2. The Position of the GFP Tag on Actin Affects the Filament Formation in Mammalian Cells
    Akira Nagasaki, Saku Kijima, Tenji Yumoto, Miku Imaizumi, Hyonchol Kim, Chikashi Nakamura and Taro Q.P. Uyeda
    Cell Structure and Function, 42(2), 131-140 (2017)
  3. Quantitative measurements of intercellular adhesion between a macrophage and cancer cells using a cup-attached AFM chip
    Hyonchol Kim, Ayana Yamagishi, Miku Imaizumi, Yui Onomura, Akira Nagasaki, Yohei Miyagi, Tomoko Okada and Chikashi Nakamura
    Colloids and Surfaces B: Biointerfaces, 155, 366-372 (2017)
  4. DNA aptamers against FokI nuclease domain for genome editing applications
    Maui Nishio, Daisuke Matsumoto, Yoshio Kato, Koichi Abe, Jinhee Lee, Kaori Tsukakoshi, Ayana Yamagishi, Chikashi Nakamura and Kazunori Ikebukuro
    Biosensors & Bioelectronics, 93, 26-31 (2017)

2016

  1. High efficiency penetration of antibody-immobilized nanoneedle thorough plasma membrane for in situ detection of cytoskeletal proteins in living cells
    Ryuzo Kawamura, Keita Shimizu, Yuta Matsumoto, Ayana Yamagishi, Yaron R. Silberberg, Masumi Iijima, Shun’ichi Kuroda, Kyoko Fukazawa, Kazuhiko Ishihara and Chikashi Nakamura*
    Journal of Nanobiotechnology, 14(1), 74 (2016)
  2. Evaluation of DNA Transcription of Living Cell During Nanoneedle Insertion
    Sung-Woong Han, Hoon-Kyu Shin, Seung-Hwan Ryu and Chikashi Nakamura
    Journal of Nanoscience and Nanotechnology, 16(8), 8674-8677 (2016)
  3. Mechanoporation of living cells for delivery of macromolecules using nanoneedle array
    Daisuke Matsumoto, Ayana Yamagishi, Megumi Saito, Ramachandra Rao Sathuluri, Yaron R. Silberberg, Futoshi Iwata, Takeshi Kobayashi and Chikashi Nakamura*
    Journal of Bioscience and Bioengineering, 122, 748-752(2016)
  4. ATP-mediated release of a DNA-binding protein from a silicon nanoneedle array
    Daisuke Matsumoto, Maui Nishio, Yoshio Kato, Wataru Yoshida, Koichi Abe, Kyoko Fukazawa, Kazuhiko Ishihara, Futoshi Iwata, Kazunori Ikebukuro, Chikashi Nakamura*
    Electrochemistry, 84(5), 305-307 (2016)

2015

  1. Oscillating high-aspect-ratio monolithic silicon nanoneedle array enables efficient delivery of functional bio-macromolecules into living cells
    Daisuke Matsumoto, Ramachandra Rao Sathuluri, Yoshio Kato, Yaron R. Silberberg,Ryuzo Kawamura, Futoshi Iwata, Takeshi Kobayashi and Chikashi Nakamura*
    Scientific Reports, 5, 15325 (2015)

2014

  1. Measurement of cell adhesion force by vertical forcible detachment using an arrowhead nanoneedle and atomic force microscopy
    Seunghwan Ryu, Yui Hashizume, Mari Mishima, Ryuzo Kawamura, Masato Tamura, Hirofumi Matsui, Michiya Matsusaki, Mitsuru Akashi and Chikashi Nakamura*
    Biochemical and Biophysical Research Communications, 451(1), 107-111 (2014)
  2. Single-cell manipulation and DNA delivery technology using atomic force microscopy and nanoneedle
    Sung-Woong Han, Chikashi Nakamura, Jun Miyake, Sang-Mok Chang and Taiji Adachi
    Journal of Nanoscience and Nanotechnology, 14(1), 57-70 (2014)
  3. Detection of microtubules in vivo using antibody-immobilized nanoneedles
    Yaron R. Silberberg, Ryuzo Kawamura, Seunghwan Ryu, Kyoko Fukazawa, Kazuhiko Ishihara and Chikashi Nakamura*
    Journal of Bioscience and Bioengineering, 117(1), 107-112 (2014)

2013

  1. Nanoneedle insertion into the cell nucleus does not induce double-strand breaks in chromosomal DNA
    Seunghwan Ryu, Ryuzo Kawamura, Ryohei Naka, Yaron R. Silberberg, Noriyuki Nakamura and Chikashi Nakamura*
    Journal of Bioscience and Bioengineering, 116(3), 391-396 (2013)
  2. Controlled cell adhesion using a biocompatible anchor for membrane-conjugated bovine serum albumin/bovine serum albumin mixed layer
    Ryuzo Kawamura, Mari Mishima, Seunghwan Ryu, Yu Arai, Motomu Okose, Yaron R. Silberberg, Sathuluri Ramachandra Rao and Chikashi Nakamura*
    Langmuir, 29(21), 6429-6433 (2013)
  3. Evaluation of the actin cytoskeleton state using an antibody-functionalized nanoneedle and an AFM
    Yaron R. Silberberg, Shingo Mieda, Yosuke Amemiya, Toshiya Sato, Takanori Kihara, Noriyuki Nakamura, Kyoko Fukazawa, Kazuhiko Ishihara, Jun Miyake and Chikashi Nakamura*
    Biosensors & Bioelectronics, 40(1), 3-9 (2013)

2012

  1. Formation of nanofilms on cell surfaces to improve the insertion efficiency of a nanoneedle into cells
    Yosuke Amemiya, Keiko Kawano, Michiya Matsusaki, Mitsuru Akashi, Noriyuki Nakamura and Chikashi Nakamura*

    Biochemical and Biophysical Research Communications, 420(3), 662-665 (2012)
  2. Mechanical force-based probing of intracellular proteins from living cells using antibody-immobilized nanoneedles
    Shingo Mieda, Yosuke Amemiya, Takanori Kihara, Tomoko Okada, Toshiya Sato, Kyoko Fukazawa, Kazuhiko Ishihara, Noriyuki Nakamura, Jun Miyake and Chikashi Nakamura*
    Biosensors & Bioelectronics, 31(1), 323-329 (2012)

2011

  1. Development of a novel biosensing system based on the structural change of a polymerized guanine-quadruplex DNA nanostructure
    Yo Morita, Wataru Yoshida, Nasa Savory, Sung Woong Han, Masayuki Tera, Kazuo Nagasawa, Chikashi Nakamura, Koji Sode, Kazunori Ikebukuro
    Biosensors & Bioelectronics, 26(12), 4837-4841 (2011)
  2. Analysis of the unbinding force between telomestatin derivatives and human telomeric G-quadruplex by atomic force microscopy
    Yosuke Amemiya, Yui Furunaga, Keisuke Iida, Masayuki Tera, Kazuo Nagasawa, Kazunori Ikebukuro and Chikashi Nakamura*
    Chemical Communications, 47(26), 7485-7487 (2011)
  3. Direct electron transfer to hydrogenase for catalytic hydrogen production using a single-walled carbon nanotube forest
    Takanori Kihara, Xue-Ying Liu, Chikashi Nakamura*, Kang-Min Park, Sung-Woong Han, Dong-Jin Qian, Kazunori Kawasaki, Nikolay A. Zorin, Satoshi Yasuda, Kenji Hata, Tatsuki Wakayama and Jun Miyake
    International Journal of Hydrogen Energy, 36(13), 7523-7529 (2011)
  4. Successive detection of insulin-like growth factor-II bound to receptors on a living cell surface using an AFM
    Sung-Woong Han, Shingo Mieda, Chikashi Nakamura*, Takanori Kihara, Noriyuki Nakamura and Jun Miyake
    Journal of Molecular Recognition, 24(1), 17-22 (2011)

2010

  1. Development of a novel method to detect intrinsic mRNA in a living cell by using a molecular beacon-immobilized nanoneedle
    Takanori Kihara, Narutoshi Yoshida, Taro Kitagawa, Chikashi Nakamura*, Noriyuki Nakamura and Jun Miyake
    Biosensors & Bioelectronics, 26(4), 1449-1454 (2010)
  2. Fabrication of hydrogenase-cationic electrolyte biohybrids at interfaces and their electrochemical properties in Langmuir-Blodgett films
    An Liu, Nikolay A. Zorin, Chikashi Nakamura, Jun Miyake and Dong-Jin Qian
    Materials Chemistry and Physics, 122, 556-562 (2010)
  3. The mechanical properties of a cell, as determined by its actin cytoskeleton, are important for nanoneedle insertion into a living cell
    Harumi Kagiwada, Chikashi Nakamura*, Takanori Kihara, Hideki Kamiishi, Keiko Kawano, Noriyuki
    Nakamura and Jun Miyake Cytoskeleton, 67(8), 496-503 (2010)
  4. Pyrroloquinoline quinone inhibits the fibrillation of amyloid proteins
    Jihoon Kim, Masaki Kobayashi, Makoto Fukuda, Daisuke Ogasawara, Natsuki Kobayashi, Sung woong Han, Chikashi Nakamura, Masaki Inada, Chisato Miyaura, Kazunori Ikebukuro and Koji Sode
    Prion, 4(1), 26-31 (2010)

2009

  1. Evaluation of the insertion efficiencies of tapered silicon nanoneedles and invasiveness of diamond nanoneedles in manipulations of living single cells
    Sung-Woong Han, Seunghwan Ryu, Taro Kitagawa, Hiroshi Uetsuka, Naoji Fujimori, Yukihiro Aoki, Ryo Ota, Yosuke Amemiya, Nobuo Shimamoto, Chikashi Nakamura* and Jun Miyake
    Archives of Histology and Cytology, 72(4/5), 261-270 (2009)
  2. The effect of amino acid substitution in the imperfect repeat sequences of a-synuclein on fibrillation
    Ryuichi Harada, Natsuki Kobayashi, Jihoon Kim, Chikashi Nakamura, Sung-Woong Han, Kazunori Ikebukuro and Koji Sode
    Biochimica et Biophysica Acta - Molecular Basis of Disease, 1792(10), 998-1003 (2009)
  3. Development of a method to evaluate caspase-3 activity in a single cell using a nanoneedle and a fluorescent probe
    Takanori Kihara, Chikashi Nakamura*, Miho Suzuki, Sung-Woong Han, Kyoko Fukazawa, Kazuhiko Ishihara and Jun Miyake
    Biosensors & Bioelectronics, 25(1), 22-27 (2009)
  4. Interfacial self-assembly of metal-mediated viologen-like coordination polyelectrolyte hybrids of the bisterpyridine ligand and their optical, electrochemical, and electrochromic properties
    Chao-Feng Zhang, An Liu, Meng Chen, Chikashi Nakamura, Jun Miyake and Dong-Jin Qian
    ACS Applied Materials & Interfaces, 1(6), 1250-1258 (2009)
  5. Monitoring of hormonal drug effect in a single breast cancer cell using an estrogen responsive GFP reporter vector delivered by a nanoneedle
    Sung-Woong Han, Chikashi Nakamura*, Yosuke Imai, Noriyuki Nakamura and Jun Miyake
    Biosensors & Bioelectronics, 24(5), 1219-1222 (2009)

2008

  1. Electrochemically driven generation of manganese(IV,V)-oxo multiporphyrin arrays and their redox properties with manganese(III) species in Langmuir-Blodgett films
    Chao-Feng Zhang, Meng Chen, Chikashi Nakamura, Jun Miyake and Dong-Jin Qian
    Langmuir, 24(23), 13490-13495 (2008)
  2. High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy
    Sung-Woong Han, Chikashi Nakamura*, Noriko Kotobuki, Ikuo Obataya, Hajime Ohgushi, Teruyuki Nagamune and Jun Miyake
    Nanomedicine: Nanotechnology, Biology and Medicine, 4(3), 215-225 (2008)
  3. Aggregation and fibrillation study of α-synuclein under applied voltage
    Yuko Osawa, Kazunori Ikebukuro, Natsuki Kobayashi, Sung-Woong Han, Chikashi Nakamura and Koji Sode
    Electrochemistry, 76(8), 614-618 (2008)
  4. A Nanoneedle can be inserted into a living cell without any mechanical stress inducing calcium ion influx
    Chikashi Nakamura*, Hideki Kamiishi, Noriyuki Nakamura and Jun Miyake
    Electrochemistry, 76 (8), 586-589 (2008)
  5. Photoinduced hydrogen evolution in an artificial system containing photosystem I, hydrogenase, methyl viologen and mercaptoacetic acid Dong Jin Qian, Ai Rong Liu,
    Chikashi Nakamura, Stephan Olav Wenk and Jun Miyake
    Chinese Chemical Letters, 19(5), 607-610 (2008)
  6. Correlation between proliferative activity and cellular thickness of human mesenchymal stem cells
    Yoshihiro Katsube, Motohiro Hirose, Chikashi Nakamura and Hajime Ohgushi
    Biochemical and Biophysical Research Communications, 368 (2), 256-260 (2008)
  7. Assembly and characterization of zinc porphyrin-hydrogenase-(poly)viologen triads on substrate surfaces
    Ai-Rong Liu, Xu Wang, Chikashi Nakamura, Jun Miyake, Nikolay A. Zorin and Dong-Jin Qian
    The Journal of Physical Chemistry C, 112(5), 1582-1588 (2008)

2007

  1. Coordination polymer nanocombs self-assembled at the water-chloroform interface
    Bing Liu, Meng Chen, Chikashi Nakamura, Jun Miyake and Dong-Jin Qian
    New Journal of Chemistry, 31(6), 1007-1012 (2007)
  2. Nanoneedle surface modification with 2-methacryloyloxyethyl phosphorylcholine polymer to reduce nonspecific protein adsorption in a living cell
    Takanori Kihara, Narutoshi Yoshida, Shingo Mieda, Kyoko Fukazawa, Chikashi Nakamura, Kazuhiko Ishihara and Jun Miyake
    NanoBiotechnology, 3(2), 127-134 (2007)
  3. Enzymatic nanolithography of FRET peptide layer using V8 protease-immobilized AFM probe
    Chikashi Nakamura*, Chie Miyamoto, Ikuo Obataya, Seiji Takeda, Masayuki Yabuta and Jun Miyake
    Biosensors & Bioelectronics, 22(9-10), 2308-2314 (2007)
  4. Simple and high-sensitivity detection of dioxin using dioxin-binding pentapeptide
    Yasuhiro Inuyama, Chikashi Nakamura*, Tomohiro Oka, Yasuhiro Yoneda, Ikuo Obataya, Noriaki Santo and Jun Miyake
    Biosensors & Bioelectronics, 22(9-10), 2093-2099 (2007)
  5. Electrochemical properties of carbon nanotubes-hydrogenase conjugates Langmuir-Blodgett films
    Ai-Rong Liu, Tatsuki Wakayama, Chikashi Nakamura, Jun Miyake, Nikolay A. Zorin and Dong-Jin Qian
    Electrochimica Acta, 52(9), 3222-3228 (2007)

2006

  1. Pyrroloquinoline quinone (PQQ) prevents fibril formation of α-synuclein
    Masaki Kobayashi, Jihoon Kim, Natsuki Kobayashi, Sungwoong Han, Chikashi Nakamura, Kazunori Ikebukuro and Koji Sode
    Biochemical and Biophysical Research Communications, 349(3), 1139-1144 (2006)
  2. Electrochemical properties of hydrogenase on glass carbon electrodes modified with carbon nanotubes
    Ai-Rong Liu, Dong-Jin Qian, Meng Chen, Tatsuki Wakayama, Chikashi Nakamura, Nikolay A. Zorin and Jun Miyake
    NanoBiotechnology, 2(3-4), 135-141 (2006)
  3. Analysis of DNA and zinc finger interactions using mechanical force spectroscopy
    Yanyan Wang, Shin-ichiro Oyokawa, Sung-Woong Han, Wei Huang, Kazunori Ikebukuro, Chikashi Nakamura* and Jun Miyake
    NanoBiotechnology, 2(3-4), 87-93 (2006)
  4. High sensitivity detection of bisphenol A using liposome chromatography
    Xue-Ying Liu, Chikashi Nakamura*, Itsuro Tanimoto, Shiro Miyake, Noriyuki Nakamura, Takashi Hirano and Jun Miyake
    Analytica Chimica Acta, 578(1), 43-49 (2006)
  5. Layer-by-layer assembly of single-walled carbon nanotube-poly(viologen) derivative multilayers and their electrochemical properties
    Xu Wang, Hong-Xiang Huang, Ai-Rong Liu, Bing Liu, Tatsuki Wakayama, Chikashi Nakamura, Jun Miyake and Dong-Jin Qian
    Carbon, 44(11), 2115-2121 (2006)
  6. Monolayers and Langmuir-Blodgett films of palladium-mediated multiporphyrin arrays with different central ion
    Dong-Jin Qian ,Hai-Tao Chen, Bing Liu, Xian-Ming Xiang, Tatsuki Wakayama, Chikashi Nakamura and Jun Miyake
    Colloids and Surfaces A: Physicochemical and Engineering Aspects, 284-285, 180-186 (2006)
  7. Monolayers, Langmuir-Blodgett films of carbon nanotubes-cytochrome c conjugates and electrochemistry
    Ai-Rong Liu, Dong-Jin Qian, Tatsuki Wakayama, Chikashi Nakamura and Jun Miyake
    Colloids and Surfaces A: Physicochemical and Engineering Aspects, 284-285, 485-489 (2006)
  8. Metal-mediated coordination polymer nanotubes of 5,10,15,20-tetrapyridylporphine and tris(4-pyridyl)-1,3,5-triazine at the water-chloroform interface
    Bing Liu, Dong-Jin Qian, Meng Chen, Tatsuki Wakayama, Chikashi Nakamura and Jun Miyake
    Chemical Communications, 30, 3175-3177 (2006)

2005

  1. Direct insertion of proteins into a living cell using an atomic force microscope with a nanoneedle
    Ikuo Obataya, Chikashi Nakamura*, Sung Woong Han, Noriyuki Nakamura and Jun Miyake
    NanoBiotechnology, 1(4), 347-352 (2005)
  2. Dioxin-binding pentapeptide for use in a high-sensitivity on-bead detection assay
    Chikashi Nakamura*, Yasuhiro Inuyama, Hiroki Goto, Ikuo Obataya, Nao Kaneko, Noriyuki Nakamura, Noriaki
    Santo and Jun Miyake Analytical Chemistry, 77(23), 7750-7757 (2005)
  3. Detection of polychlorinated biphenyls using an antibody column in tandem with a fluorescent liposome column: Effect of albumin on phospholipase A2-catalyzed membrane leakage Xue-Ying Liu,
    Chikashi Nakamura*, Noriyuki Nakamura, Takashi Hirano, Toshio Shinbo and Jun Miyake
    Journal of Chromatography A, 1087(1-2), 229-235 (2005)
  4. Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness
    Sung Woong Han, Chikashi Nakamura*, Ikuo Obataya, Noriyuki Nakamura and Jun Miyake
    Biochemical and Biophysical Research Communications, 332(3), 633-639 (2005)
  5. Controllable growth of well-defined regular multiporphyrin array nanocrystals at the water-chloroform interface
    Bing Liu, Dong-Jin Qian, Hong-Xiang Huang, Tatsuki Wakayama, Shigeki Hara, Wei Huang, Chikashi Nakamura and Jun Miyake
    Langmuir, 21(11), 5079-5084 (2005)
  6. A molecular delivery system by using AFM and nanoneedle
    Sung-Woong Han, Chikashi Nakamura*, Ikuo Obataya, Noriyuki Nakamura and Jun Miyake
    Biosensors & Bioelectronics, 20(10), 2120-2125 (2005)
  7. Mechanical sensing of the penetration of various nanoneedles into a living cell using atomic force microscopy
    Ikuo Obataya, Chikashi Nakamura*, Sung Woong Han, Noriyuki Nakamura and Jun Miyake
    Biosensors & Bioelectronics, 20(8), 1652-1655 (2005)
  8. Nanoscale operation of a living cell using an atomic force microscope with a nanoneedle
    Ikuo Obataya, Chikashi Nakamura*, SungWoong Han, Noriyuki Nakamura and Jun Miyake
    Nano Letters, 5(1) 27-30 (2005)

2004

  1. Measurement of intramolecular energy dissipation and stiffness of a single peptide molecule by magnetically modulated atomic force microscopy
    Masami Kageshima, Seiji Takeda, Arkadiusz Ptak, Chikashi Nakamura, Suzanne P. Jarvis, Hiroshi Tokumoto and Jun Miyake
    Japanese Journal of Applied Physics, 43(12A), pp.L1510-L1513 (2004)
  2. Preparation and electrochemical properties of organic-inorganic hybrids with the use of alkylammonium or alkylviologen cations and polyoxometalate anions
    Dong-Jin Qian, Hong-Xiang Huang, Wei Huang, Tatsuki Wakayama, Chikashi Nakamura and Jun Miyake
    Colloids and Surfaces A: Physicochemical and Engineering Aspects, 248(1-3), 85-91 (2004)
  3. Development of a herbicide biosensor using a peptide receptor screened from a combinatorial library
    Ikuo Obataya, Chikashi Nakamura*, Hideyuki Enomoto, Takayuki Hoshino, Noriyuki Nakamura and Jun Miyake
    Journal of Molecular Catalysis B: Enzymatic, 28(4-6), 265-271 (2004)
  4. Quartz crystal microbalance and electrochemical studies on the electrode modified by layer-by-layer multilayers of viologen polyelectrolytes
    Hong-Xiang Huang, Dong-Jin Qian, Noriyuki Nakamura, Chikashi Nakamura, Tatsuki Wakayama and Jun Miyake
    Electrochimica Acta, 49(9-10), 1491-1498 (2004)
  5. Mechanical force analysis of peptide interactions using atomic force microscopy
    Chikashi Nakamura*, Seiji Takeda, Masami Kageshima, Miyuki Ito, Naoki Sugimoto, Kazuko Sekizawa and Jun Miyake
    Biopolymers (Peptide Science), 76(1), 48-54 (2004)

2003

  1. Analysis of the structure and morphology of oligopeptide SAM using XPS and AFM
    S.H. Song, K.M. Park, S.M. Chang, C. Nakamura, J. Miyake and W.S. Kim
    Molecular Crystals and Liquid Crystals, 407(1), 135-140 (2003)
  2. Lithographing of biomolecules on a substrate surface using an enzyme-immobilized AFM tip
    Seiji Takeda, Chikashi Nakamura*, Chie Miyamoto, Noriyuki Nakamura, Masami Kageshima, Hiroshi Tokumoto and Jun Miyake
    Nano Letters, 3(11), 1471-1474 (2003)
  3. PCR-based ribosomal DNA detection technique for microalga (Heterosigma carterae) causing red tide and its application to a biosensor using labeled probe
    Ryoichi Asai, Kozue Ootani, Yoko Nomura, Chikashi Nakamura, Kazunori Ikebukuro, Yoshiko Arikawa, Jun Miyake and Isao Karube
    Marine Biotechnology, 5(5), 417-423 (2003)
  4. Effect of lipid composition on phospholipase A2-catalyzed membrane leakage in immobilized liposomes: Sensitization for polychlorinated biphenyls detection with antibody affinity column tandem with fluorescent liposome column
    Xue-Ying Liu, Chikashi Nakamura*, Miki Hasegawa and Jun Miyake
    Electrophoresis, 24(18), 3165-3171 (2003)
  5. Synthesis and multilayer assembly of multiporphyrin arrays at the water-chloroform interface
    Dong-Jin Qian, Chikashi Nakamura, Tatsuki Wakayama and Jun Miyake
    Journal of Porphyrins and Phthalocyanines, 7(6), 415-423 (2003)
  6. A polymerase chain reaction-based ribosomal DNA detection technique using a surface plasmon resonance detector for a red tide causing microalga, Alexandrium affine
    Ryoichi Asai, Keijoro Nakanishi, Chikashi Nakamura, Kazunori Ikebukuro, Jun Miyake and Isao Karube
    Phycological Research, 51(2), 118-125 (2003)
  7. Unfolding process of a single peptide molecule on a substrate was investigated by atomic force microscope
    Seiji Takeda, Arkadiusz Ptak , Masami Kageshima, Hiroshi Tokumoto, Chikashi Nakamura and Jun Miyake
    Surface Science, 532-535, 244-248 (2003)
  8. A bioassay to detect contaminant-induced messenger RNA using a transcriptomic approach: Detection of RT-PCR-amplified single-stranded DNA based on the SPR sensor in cyanobacteria
    Ryoichi Asai, Chikashi Nakamura, Kazunori Ikebukuro, Isao Karube and Jun Miyake
    Analytical Letters, 36(8), 1475-1491 (2003)
  9. Rapid and specific detection of herbicides using a self-assembled photosynthetic reaction center from purple bacterium on an SPR chip
    Chikashi Nakamura*, Miki Hasegawa, Noriyuki Nakamura and Jun Miyake
    Biosensors & Bioelectronics, 18(5-6), 599-603 (2003)
  10. "Single" Pd(0) atom encapsulated in multiporphyrin arrays as a highly efficient heterogeneous catalyst
    Dong-Jin Qian, Tatsuki Wakayama, Chikashi Nakamura and Jun Miyake
    Journal of Physical Chemistry B, 107(15), 3333-3335 (2003)
  11. Conventional detection of 2,4-dinitrophenol using quartz crystal microbalance
    Jong-Won Park, Shigeru Kurosawa, Hidenobu Aizawa, Dae-Sang Han, Minoru Yoshimoto, Chikashi Nakamura, Jun Miyake and Sang-Mok Chang
    IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 50(2), 193-195 (2003)
  12. Electrochemical hydrogen evolution by use of a glass carbon electrode sandwiched with clay, poly(butylviologen) and hydrogenase
    Dong-Jin Qian, Chikashi Nakamura, Stephan-Olav Wenk, Tatsuki Wakayama, Nikolay Zorin and Jun Miyake
    Materials Letters, 57(5-6), 1130-1134 (2003)

2002

  1. Palladium-mediated stepwise assembly of three-dimensional organized multiporphyrin arrays directly on solid substrates
    Dong-Jin Qian, Chikashi Nakamura, Takao Ishida, Stephen-Olav Wenk, Tatsuki Wakayama, Seiji Takeda and Jun Miyake
    Langmuir, 18(26), 10237-10242 (2002)
  2. Biomolecular device for photoinduced hydrogen production
    Stephan-Olav Wenk, Dong-Jin Qian, Tatsuki Wakayama, Chikashi Nakamura, Nikolay Zorin, Matthias Roegner and Jun Miyake
    International Journal of Hydrogen Energy, 27(11-12), 1489-1493 (2002)
  3. Photoinduced hydrogen evolution by use of porphyrin, EDTA, viologens and hydrogenase in solutions and Langmuir-Blodgett films
    Dong-Jin Qian, Stephan-Olav Wenk, Chikashi Nakamura, Tatsuki Wakayama, Nikolay Zorin and Jun Miyake
    International Journal of Hydrogen Energy, 27(11-12), 1481-1487 (2002)
  4. Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis
    Ryoichi Asai, Chikashi Nakamura, Kazunori Ikebukuro, Isao Karube and Jun Miyake
    Biotechnology Letters, 24(20), 1677-1682 (2002)
  5. Quartz crystal microbalance sensor targeting low molecular weight compounds using oligopeptide binder and peptide-immobilized latex beads
    Chikashi Nakamura*, Seong-Hun Song, Sang-Mok Chang, Naoki Sugimoto and Jun Miyake
    Analytica Chimica Acta, 469(2), 183-188 (2002)
  6. A hydrogen biosensor made of clay, poly(butylviologen), and hydrogenase sandwiched on a glass carbon electrode
    Dong-Jin Qian, Chikashi Nakamura, Stephan-Olav Wenk, Hiroshi Ishikawa, Nikolay Zorin and Jun Miyake
    Biosensors & Bioelectronics, 17(9), 789-796 (2002)
  7. mmobilized fluorescent liposome column for bioanalysis and signal amplification
    Xue-Ying Liu, Chikashi Nakamura and Jun Miyake
    Biological Systems Engineering ACS Symposium Series 830,
    Edited by Mark R. marten, Tai Hyun Park and Teruyuki Nagamune
    Published by the American Chemical Society, Chapter 21, pp. 259-270 (2002)
  8. Design and activity of antimicrobial peptides against sporogonic-stage parasites causing Murine Malarias
    Romanico B. G. Arrighi, Chikashi Nakamura, Jun Miyake, Hilary Hurd and J. Grant Burgess
    Antimicrobial Agents and Chemotherapy, 46(7), 2104-2110 (2002)
  9. Immobilized liposome chromatography to study drug-membrane interactions: Correlation with drug absorption in humans
    Xue-Ying Liu, Chikashi Nakamura, Qing Yang, Naoki Kamo and Jun Miyake
    Journal of Chromatography A, 961(1), 113-118 (2002)