Name Position Email(*) Dr. Masaya Suzuki Group Leader masaya-suzuki Dr. Hanako Mochimaru Senior Researcher h-mochimaru Dr. Taiki Katayama Chief Senior Researcher katayama.t Dr. Daisuke Mayumi Senior Researcher mayumi-daisuke Dr. Masanori Kaneko Senior Researcher m-kaneko Dr. Konomi Suda Researcher suda-konomi Dr. Yusuke Miyajima Researcher yusuke.miyajima Dr. Susumu Sakata Invited Senior Researcher su-sakata Ms. Yumi Shinotsuka Technical Staff Ms. Misa Takenouchi Technical Staff Ms. Yukiko Hoshi Technical Staff Ms. Rieko Iwanami Technical Staff Ms. Kazuko Manpuku Technical Staff Mr. Satoshi Hamasaki Technical Staff Ms. Mari Oohara Technical Staff Ms. Ayami Ishikawa Technical Staff (*)@aist.go.jp

Dr. Masaya Suzuki
	Research Fields Agricultural Engineering, Material Chemistry Research Topics Development of carbon dioxide application equipment needed to replace microbial activity. Development of dehumidification equipment for facility horticulture. Comment I will develop low-cost horticultural equipment that can contribute to farmers! Publication

Dr. Hanako Mochimaru

Research Fields Geomicrobiology, Anaerobic microbiology Research Topics Microbial diversity and methanogenic potential in deep subsurface gas fields. Isolation of novel microorganisms from deep subsurface environments. Crude oil biodegradation coupled to methane production in high-temperature oil reservoirs. Comment Between you and me, my special interest is to find new methanogens. Publication Tamazawa S., Mayumi D., Mochimaru H., Sakata S., Maeda H., Wakayama T., Ikarashi M., Kamagata Y., Tamaki H. (2017) Petrothermobacter organivorans gen. nov., sp. nov., a novel thermophilic, strictly anaerobic bacterium of the phylum Deferribacteres isolated from a deep subsurface oil reservoir. International Journal of Systematic and Evolutionary Microbiology 67, 3982-3986. Mayumi D., Mochimaru H., Tamaki H., Yamamoto K., Yoshjoka H., Suzuki Y., Kamagata Y., Sakata S.(2016) Methane production from coal by a single methanogen. Science354 (6309), 222-225. Mochimaru H., Tamaki H., Katayama T., Imachi H., Sakata S., Kamagata Y. (2016) Methanomicrobium antiquum sp. nov., a hydrogenotrophic methanogen isolated from deep sedimentary aquifers in a natural gas field. International Journal of Systematic and Evolutionary Microbiology 66, 4873-4877. Yoshioka H, Mochimaru H, Sakata S. Takeda H, Yoshida S.(2015) Methane production potential of subsurface microbes in Pleistocene sediments from a natural gas field of the dissolved-in-water type, central Japan. Chemical Geology 419, 92-101. Katayama T., Yoshioka H., Mochimaru H., Muramoto Y., Usami J., Ikeda H., Kamagata Y., Sakata S. (2014) Methanohalophilus levihalophilus gen. nov., sp. nov., a methylotrophic methanogen isolated from deep aquifers in a natural gas field, and an emended description of the genus Methanohalophilus. International Journal of Systematic and Evolutionary Microbiology, 64, 2089-2093. Nakamura K., Takahashi A., Mori C., Tamaki H., Mochimaru H., Nakamura K., Takamizawa K., Kamagata Y. (2013) Methanothermobacter tenebrarum sp. nov., a hydrogenotrophic thermophilic methanogen isolated from gas-associated formation water of a natural gas field in Japan. International Journal of Systematic and Evolutionary Microbiology 63, 715-722. Nakamura K., Tamaki　H., Kang M. S., Mochimaru H., Lee S. T., Nakamura K., Kamagata Y. (2011) A Six-well Plate Method: Less Laborious and Effective Method for Cultivation of Obligate Anaerobic Microorganisms. Microbes & Environments 26, 301-306. Nakamura H., Narihiro T., Tsuruoka N., Mochimaru H., Matsumoto R., Tanabe Y., Hagiya K., Ikeba K., Maruyama A., Hanada S. (2011) Evaluation of the aflatoxin biosynthetic genes for identification of the Aspergillus section Flavi. Microbes & Environments 26, 367-369. Mayumi D., Mochimaru H., Yoshioka H., Sakata S., Maeda H., Miyagawa Y., Ikarashi M., Takeuchi M., Kamagata Y. (2011) Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field (Japan). Environmental Microbiology 13, 1995-2006. Mochimaru H., Tamaki H., Hanada S., Imachi H., Nakamura K., Sakata S., Kamagata Y. (2009) Methanolobus profundi sp. nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field. International Journal of Systematic and Evolutionary Microbiology 59, 714-718. Miyashita A, Mochimaru H, Kazama H, Ohashi A, Yamaguchi T, Nunoura T, Horikoshi K, Takai K, Imachi H. (2009) Development of 16S rRNA gene-targeted primers for detection of archaeal anaerobic methanotrophs (ANMEs). FEMS Microbiol Lett.297, 31-37. Mochimaru H., Uchiyama H., Yoshioka H., Imachi H., Hoaki T., Tamaki H., Nakamura K., Sekiguchi Y., Kamagata Y. (2007) Methanogen diversity in deep subsurface gas-associated water at the Minami-kanto gas field in Japan. Geomicrobiology Journal 24. 93-100. Mochimaru H., Yoshioka H., Tamaki H., Nakamura K., Kaneko N., Sakata S., Imachi H., Sekiguchi Y., Uchiyama H., Kamagata Y. (2007) Microbial diversity and methanogenic potential in a high temperature natural gas field in Japan. Extremophiles 11, 453-461.

Dr. Taiki Katayama

Research Fields Geomicrobiology, Microbial Physiology Research Topics Biogenic methane production in gas hydrate-bearing subseafloor sediments, natural gas field of dissolved-in-water type and sedimentary coastal sediments: studies on microbial community structure and distribution, methanogenic activity and pathway and environmental factors that may influence methanogenesis. Cultivation of as-yet-unknown bacteria and archaea in deep subsurface environments. Development of enhanced microbial recovery in natural gas field, Japan. Comment Art is I; Science is we. (Claude Bernard) Publication Katayama T., Nobu M. K., Imachi H., Hosogi N., Meng X.-Y., Morinaga K., Yoshioka H., Takahashi H. A., Kamagata Y., Tamaki H. (2024) A Marine Group A isolate relies on other growing bacteria for cell wall formation. Nature Microbiology, https://doi.org/10.1038/s41564-024-01717-7. Kawamoto H., Watanabe M., Mochimaru H., Nakahara N., Meng X.-Y., Sakamoto S., Morinaga K., Katayama T., Yoshioka H., Nomura N., Tamaki H. (2024) Atrimonas thermophila gen. nov., sp. nov., a novel anaerobic thermophilic bacterium of the phylum Atribacterota isolated from deep subsurface gas field and proposal of Atrimonadaceae fam. nov. within the class Atribacteria in the phylum Atribacterota. Systematic and Applied Microbiology 47, https://doi.org/10.1016/j.syapm.2024.126515. Gotore O., Watanabe M., Okano K., Miyata N., Katayama T., Yasutaka T., Semoto Y., Hamai T. (2024) Effects of batch and continuous-flow operation on biotreatment of Mn(II)-containing mine drainage. Journal of Environmental Sciences 152, 401-415, https://doi.org/10.1016/j.jes.2024.05.038. Katayama T., Ikawa R., Koshigai M., Sakata S. (2023) Microbial methane formation in deep aquifers associated with the sediment burial history at a coastal site. Biogeosciences 20, 5199–5210, https://doi.org/10.5194/bg-20-5199-2023. Hirakata Y., Mei R., Morinaga K., Katayama T., Tamaki H., Meng X.-Y, Watari T., Yamaguchi T., Hatamoto M., Nobu K. M. (2023) Identification and cultivation of anaerobic bacterial scavengers of dead cells. The ISME Journal 17, 2279–2289, https://doi.org/10.1038/s41396-023-01538-2. Katayama T., Yoshioka H., Kaneko M., Amo M., Fujii T., Takahashi H. A., Yoshida S., Sakata S. (2022) Cultivation and biogeochemical analyses reveal insights into methanogenesis in deep subseafloor sediment at a biogenic gas hydrate site. The ISME Journal, https://doi.org/10.1038/s41396-021-01175-7. Yoshikawa M., Zhang M., Kawabe Y., Katayama T.(2021) Effects of ferrous iron supplementation on reductive dechlorination of tetrachloroethene and on methanogenic microbial community. FEMS Microbiology Ecology, Volume 97, Issue 5, https://doi.org/10.1093/femsec/fiab069. Takeuchi M., Fujiwara-Nagata E., Katayama T. et al. (2021) Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum. Scientific Reports 11, 7518. https://doi.org/10.1038/s41598-021-87167-1. Katayama T., Nobu M., Kusada H., Meng X., Hosogi N., Uematsu K., Yoshioka H., Kamagata Y., and Tamaki H. (2020) Isolation of a member of the candidate phylum ‘Atribacteria’ reveals a unique cell membrane structure. Nature Communications, DOI: 10.1038/s41467-020-20149-5. Katayama T., Yoshioka H., Yamanaka T., Mio Takeuchi, Muramoto Y., Usami J., Ikeda H. and Sakata S. (2019) Microbial community structure in deep natural gas-bearing aquifers subjected to sulfate-containing fluid injection. Journal of Bioscience and Bioengineering 127, 45-51. Mochimaru H., Tamaki H., Katayama T., Imachi H., Sakata S., Kamagata Y. (2016) Methanomicrobium antiquum sp. nov., a hydrogenotrophic methanogen isolated from deep sedimentary aquifers in a natural gas field. International Journal of Systematic and Evolutionary Microbiology 66, 4873-4877. Katayama T., Yoshioka H., Takahashi H.A., Amo M., Fujii T., Sakata S.(2016) Changes in microbial communities associated with gas hydrates in subseafloor sediments from the Nankai Trough.FEMS Microbiology Ecology 92, fiw093. Takeuchi M., Yamagishi T., Kamagata Y., Oshima K., Hattori M., Katayama T., Hanada S., Tamaki H., Marumo K., Maeda H., Nedachi M., Iwasaki W., Suwa Y., Sakata S. (2015) Tepidicaulis marinus gen. nov., sp. nov., a novel marine bacterium reducing nitrate to nitrous oxide strictly under microaerobic conditions. International Journal of Systematic and Evolutionary Microbiology65, 1749-1754. Kanno M., Katayama T. (equal contribution), Morita N., Tamaki H., Hanada S., Kamagata Y. (2015) Catenisphaera adipataccumulans gen. nov., sp. nov., a member of the family Erisipelotrichaceae isolated from an anaerobic digester. International Journal of Systematic and Evolutionary Microbiology 65, 805-810. Katayama T., Yoshioka H., Muramoto Y., Usami J., Fujiwara K., Yoshida S., Kamagata Y., Sakata S. (2015) Physicochemical impacts associated with natural gas development on methanogenesis in deep sand aquifers. The ISME Journal 9, 436-446. Katayama T., Yoshioka H., Mochimaru H., Muramoto Y., Usami J., Ikeda H., Kamagata Y., Sakata S. (2014) Methanohalophilus levihalophilus gen. nov., sp. nov., a methylotrophic methanogen isolated from deep aquifers in a natural gas field, and an emended description of the genus Methanohalophilus. International Journal of Systematic and Evolutionary Microbiology, 64, 2089-2093. Takeuchi M., Katayama T., Yamagishi T., Hanada S., Tamaki H., Kamagata Y., Oshima K., Hattori M., Marumo K., Nedachi M., Maeda H., Suwa Y., Sakata S. (2014)Methyloceanibacter caenitepidi gen. nov., sp. nov., a novel facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent. International Journal of Systematic and Evolutionary Microbiology 64, 462-468. Katayama T., Kanno M., Morita N., Hori T., Narihiro T., Mitani Y., Kamagata Y. (2014) An oleaginous bacterium that accumulates long-chain free fatty acids in its cytoplasm. Applied and Environmental Microbiology 80, 1126-1131. Kanno M., KatayamaT. (equal contribution), Tamaki H., Mitani Y., Mou K., Hori T., Narihiro T., Morita N., Yumoto I., Kimura N., Hanada S., Kamagata Y. (2013) Isolation of butanol- and isobutanol-tolerant bacteria and physiological characterization of their butanol tolerance. Applied and Environmental Microbiology 79, 6998-7005. Puspita I.D., Uehara M., Katayama T., Kikuchi Y., Kitagawa W., Kamagata Y., Asano K., Nakatsu C.H., Tanaka, M. (2013) Resuscitation promoting factor (Rpf) from Tomitella biformata AHU1821T promotes growth and resuscitates non-dividing cells. Microbes and Environments 28, 58-64. Katayama T., Tanaka M. (2012) Microorganisms in a permafrost ice wedge and their resuscitation promoting factors. Chapter 10, pp.177-188 in Cold-Adapted Microorganisms (Edited by Yumoto I.) Caister Academic Press. Pudjiraharti S., Takesue N., Katayama T., Lisdiyanti P., Hanafi M., Tanaka M., Sone T., Asano K. (2011) Actinomycete Nonomuraea sp. isolated from Indonesian soil is a new producer of inulin fructotransferase. Journal of Bioscience and Bioengineering 111, 671-674. Katayama T., Kato T., Tanaka M., Douglas T. A.. Brouchkov A., Abe A., Sone T., Fukuda M. Asano K. (2010) Tomitella biformata gen. nov., sp. nov., a novel member of the suborder Corynebacterineae isolated from a permafrost ice　wedge. International Journal of Systematic and Evolutionary Microbiology 60, 2803-2807. Katayama T., Kato T., Tanaka M., Douglas T. A.. Brouchkov A., Fukuda M. Tomita F., Asano K. (2009) Glaciibacter superstes gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from a permafrost ice wedge. International Journal of Systematic and Evolutionary Microbiology 59, 482-486. Katayama T., Tanaka M., Moriizumi J., Nakamura T., Brouchkov A., Douglas T.A., Fukuda M., Tomita F., Asano K. (2007) Phylogenetic analysis of bacteria preserved in a permafrost ice wedge for 25,000 years. Applied and Environmental Microbiology 73, 2360-2363.

Dr. Daisuke Mayumi

Research Fields Geomicrobiology Research Topics Elucidation of methanogenic crude oil biodegradation in oil reservoirs. Evaluation of effects of geological CO2 storage on the microbial ecosystem in oil reservoirs. Identification of novel anaerobic microbes in oil reservoirs. Stable isotope analyses addressing the process of the methane hydrate production. Comment I seek the possibility of microbes in deep subsurface for industry. Publication Kurth J.M., Nobu M.K., Tamaki H., de Jonge N., Berger S., Jetten S.M., Yamamoto K., Mayumi D., Sakata S., Bai L., Cheng L., Nielsen J.L., Kamagata Y., Wagner T., Welte C.U. (2021) Methanogenic archaea use a bacteria-like methyltransferase system to demethoxylate aromatic compounds.The ISME Journal, 15, 3549–3565. https://doi.org/10.1038/s41396-021-01025-6. Suda K., Ikarashi M., Tamaki H., Tamazawa S., Sakata S., Maeda H., Kamagata Y., Kaneko M., Ujiie T., Shinotsuka Y., Wakayama T., Iwama H., Mayumi D., Yonebayashi H. (2021) Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections. Journal of Petroleum Science and Engineering, https://doi.org/10.1016/j.petrol.2021.108458. Sakamoto S., Nobu M.K., Mayumi D., Tamazawa S., Kusada H., Ikarashi M., Wakayama T., Iwama H., Yonebayashi H., Maeda H., Sakata S., Tamura T., Nomura N., Kamagata Y., Tamaki H. (2021) Koleobacter methoxysyntrophicus gen. nov., sp. nov., a novel anaerobic bacterium isolated from deep subsurface oil field and proposal of Koleobacteraceae fam. nov. and Koleobacterales ord. nov. within the class Clostridia of the phylum Firmicutes. Systematic and Applied Microbiology, https://doi.org/10.1016/j.syapm.2020.126154. Aoyagi T., Inaba T., Aizawa H., Mayumi D., Sakata S., Charfi A., Suh C., Lee J., Sato Y., Ogata A., Habe H., Hori T. (2020) Unexpected diversity of acetate degraders in anaerobic membrane bioreactor treating organic solid waste revealed by high-sensitivity stable isotope probing. Water Research 176, 115750. Xiyang Dong, Chris Greening, Jayne E. Rattray, Anirban Chakraborty, Maria Chuvochina, Daisuke Mayumi, Jan Dolfing, Carmen Li, James M. Brooks, Bernie B. Bernard, Ryan A. Groves, Ian A.Lewis & Casey R. J. Hubert (2019). Metabolic potential of uncultured bacteria and archaea associated with petroleum seepage in deep-sea sediments. Nature Communications 10, 1816, DOI: 10.1038/s41467-019-09747-0. Tamazawa S., Mayumi D., Mochimaru H., Sakata S., Maeda H., Wakayama T., Ikarashi M., Kamagata Y., Tamaki H. (2017) Petrothermobacter organivorans gen. nov., sp. nov., a novel thermophilic, strictly anaerobic bacterium of the phylum Deferribacteres isolated from a deep subsurface oil reservoir. International Journal of Systematic and Evolutionary Microbiology 67, 3982-3986. Mayumi D., Mochimaru H., Tamaki H., Yamamoto K., Yoshjoka H., Suzuki Y., Kamagata Y., Sakata S.(2016) Methane production from coal by a single methanogen. Science354 (6309), 222-225. Mayumi D., Dolfing, J., Sakata, S., Maeda, H., Miyagawa, Y., Ikarashi, M., Tamaki, H., Takeuchi, M., Nakatsu, CH., Kamagata, Y. (2013) Carbon dioxide concentration dictates alternative methanogenic pathways in oil reservoirs. Nature Communications 4:1998 doi: 10.1038/ncomms2998. Mayumi D., Mochimaru, H., Yoshioka, H., Sakata, S., Maeda, H., Miyagawa, Y., Ikarashi, M., Takeuchi, M., Kamagata, Y. (2010) Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field (Japan). Environmental Microbiology 13(8), 1995-2006. Mayumi D., Yoshimoto, T., Uchiyama, H., Nomura N., Nakajima-Kambe T. (2010) Seasonal change in methanotrophic diversity and populations in a rice field soil assessed by DNA-stable isotope probing and quantitative real-time PCR. Microbes and Environments 25, 156-163. Mayumi D., Akutsu-Shigeno Y., Uchiyama H., Nomura N., Nakajima-Kambe T. (2008) Identification and characterization of novel poly(DL-lactic acid) depolymerases from metagenome. Applied Microbiology and Biotechnology 79, 743-750.

Dr. Masanori Kaneko

Research Fields Organic Geochemistry, Biogeochemistry Research Topics Development of application of the Function-Specific-compound-analysis. Studies on biogeochemical processes based on biomarker and isotope analyses. Comment I'm trying to find something new in the biogeochemical cycle by the analytical developments and its applications. Publication 　　　　　　　　　　　　　　　　 Katayama T., Yoshioka H., Kaneko M., Amo M., Fujii T., Takahashi H.A., Yoshida S., Sakata S. (2022) Cultivation and biogeochemical analyses reveal insights into methanogenesis in deep subseafloor sediment at a biogenic gas hydrate site. The ISME Journal, https://doi.org/10.1038/s41396-021-01175-7. Urai A., Matsushita M., Park H.-D, Imachi H., Ogawara M., Iwata H., Kaneko M., Ogawa, N. O., Ohkouchi N. and Takano Y. (2021) Detection of planktonic coenzyme factor 430 in a freshwater lake: small‑scale analysis for probing archaeal methanogenesis. Progress in Earth and Planetary Science 8:62, https://doi.org/10.1186/s40645-021-00450-7. Kaneko M., Takano Y., Kamo M., Morimoto K., Nunoura T., Ohkouchi N. (2021) Insights into the Methanogenic Population and Potential in Subsurface Marine Sediments Based on Coenzyme F430 as a Function-Specific Biomarker. JACS Au 2021, 1(10), 1743–1751, https://doi.org/10.1021/jacsau.1c00307. Suda K., Ikarashi M., Tamaki H., Tamazawa S., Sakata S., Maeda H., Kamagata Y., Kaneko M., Ujiie T., Shinotsuka Y., Wakayama T., Iwama H., Mayumi D., Yonebayashi H. (2021) Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections. Journal of Petroleum Science and Engineering, https://doi.org/10.1016/j.petrol.2021.108458. Heuer V. B., Inagaki F., Morono Y., Kubo Y., Spivack A. J., Viehweger B., Treude T., Beulig F., Schubotz F., Tonai S., Bowden S. A., Cramm M., Henkel S., Hirose T., Homola K., Hoshino T., Ijiri A., Imachi H., Kamiya N., Kaneko M., Lagostina L., Manners H., McClelland H. -L., Metcalfe K., Okutsu N., Pan D., Raudsepp M. J., Sauvage J., Tsang M. -Y., Wang D. T., Whitaker E., Yamamoto Y., Yang K., Maeda L., Adhikari R. R., Glombitza C., Hamada Y., Kallmeyer J., Wendt J., Wörmer L., Yamada Y., Kinoshita M., Hinrichs K. -U. (2020) Temperature limits to deep subseafloor life in the Nankai Trough subduction zone. Science, Vol. 370, pp. 1230-1234. Yoshinori Takano, Yoshito Chikaraishi, Hiroyuki Imachi, Yosuke Miyairi, Nanako O. Ogawa, Masanori Kaneko, Yusuke Yokoyama, Martin Krüger, Naohiko Ohkouchi (2018) Insight into anaerobic methanotrophy from 13C/12C-amino acids and 14C/12C-ANME cells in seafloor microbial ecology. Scientific reports 8:14070, DOI:10.1038/s41598-018-31004-5. Kaneko, M., Takano, Y., Ogawa, N.O., Sato, Y., Yoshida, N., Ohkouchi, N. (2016) Estimation of methanogenesis by quantification of coenzyme F430 in marine sediments. Geochemical Journal, 50(6), 453-460. Inagaki, F., Hinrichs, K., U., Kubo, Y., Bowles, W., M., Heuer, V., B., Hong, L., W., Hoshino, T., Ijiri, A., Imachi, H., Ito, M., Kaneko, M., Lever, A., M., Lin, S., Y., Methe, A., B., Morita, S., Morono, Y., Tanikawa, W., Bihan, M., Bowden, A., S., Elvert, M., Glombitza, C., Gross, D., Harrington, J., G., Hori, T., Li, K., Limmer, D., Liu, H., C., Murayama, M., Ohkouchi, N., Ono, S., Park, S., Y., Phillips, C., S., Prieto-Mollar, X., Purkey, M., Riedinger, N., Sanada, Y., Sauvage, J., Snyder, G., Susilawati, R., Takano, Y., Tasumi, E., Terada, T., Tomaru, H., Trembath-Reichert, E., Wang, T., D., Yamada, Y.(2015) Exploring deep microbial life in coal-bearing sediment down to similar to 2.5 km below the ocean floor. Science 349, 420-424. Kaneko, M., Takano, Y., Chikaraishi, Y., Ogawa, N.O., Asakawa, S., Watanabe, K., Shima, S., Krueger, M., Matsushita, M., Kimura, H., and Ohkouchi, N. (2014) Quantitative analysis of coenzyme F430 in environmental samples: a new diagnostic tool for methanogenesis and anaerobic methane oxidation. Analytical Chemistry, 86(7), 3633-3638. 　　　　　　　　 Kaneko, M. and Poulson, S.R. (2013) The rate of oxygen isotope exchange between nitrate and water. Geochimica et Cosmochimica Acta, 118, 148-156. Kaneko, M., Naraoka, H., Takano, Y., and Ohkouchi, N. (2013) Distribution and isotopic signatures of archaeal lipid biomarkers associated with gas hydrate occurrences on the northern Cascadia Margin. Chemical Geology, 343, 76-84. Takano, Y., Kaneko, M., Kahnt, J., Imachi, H., Shima, S., and Ohkouchi, N. (2013) Detection of coenzyme F430 in deep sea sediments: A key molecule for biological methanogenesis. Organic Geochemistry, 58, 137-140. Nishizawa, M., Koba, K., Makabe, A., Yoshida, N., Kaneko, M., Hirao, S., Ishibashi, J., Yamanaka, T., Shibuya, T., Kikuchi, T., Hirai, M., Miyazaki, J., Nunoura, T., Takai, K. (2013) Nitrification-driven forms of nitrogen metabolism in microbial mat communities thriving along an ammonium-enriched subsurface geothermal stream. Geochimica et Cosmochimica Acta, 113, 152-173. Chikaraishi Y., Kaneko, M., and Ohkouchi N. (2012) Stable hydrogen and carbon isotopic compositions of long-chain (C21-C33) n-alkanes and n-alkenes in insects. Geochimica et Cosmochimica Acta, 95(15), 53-62. Igisu, M., Takai, K., Ueno, Y., Nishizawa, M., Nunoura, T., Hirai, M., Kaneko, M., Naraoka, H., Shimojima, M., Hori, K., Nakashima, S., Ohta, H., Maruyama, S., and Isozaki, Y. (2012) Domain-level identification and quantification of relative prokaryotic cell abundance in microbial communities by Micro-FTIR spectroscopy. Environmental Microbiology Reports, 4, 42-49. Kaneko, M. and Poulson, S. (2012) Rate of oxygen isotope exchange between selenate and water. Environmental Science and Technology, 46, 4539-4545. Kaneko, M., Kitajima, F., and Naraoka, H. (2011) Stable hydrogen isotope measurement of archaeal ether-bound hydrocarbons. Organic Geochemistry, 42, 166-172. Kaneko, M., Oba, Y., and Naraoka, H. (2010) Vertical carbon isotope change in acetate in a surface sediment from the northwestern Pacific Ocean. Research in Organic Geochemistry, 26, 73-79. Kaneko, M., Shingai, H., Pohlman, J.W., and Naraoka, H. (2010) Chemical and　isotopic signature of bulk organic matter and hydrocarbon biomarkers within mid-slope accretionary sediments of the northern Cascadia margin gas hydrate system. Marine Geology, 275, 166-177. Pohlman, J.W., Kaneko, M., Heuer, V.B., Coffin, R.B., and Whiticar, M. (2009) Methane sources and production in the northern Cascadia margin gas hydrate system. Earth and Planetary Science Letters, 287, 504-512.

Dr. Konomi Suda

Research Fields Geochemistry, History of the Earth Research Topics Methanogenic crude oil biodegradation in oil reservoirs. Origin of low-molecular-weight organic compounds in serpentinite-hosted hydrothermal systems. Comment I am studying to make clear the relationships between rock, water and life. Publication Suda K., Ikarashi M., Tamaki H., Tamazawa S., Sakata S., Maeda H., Kamagata Y., Kaneko M., Ujiie T., Shinotsuka Y., Wakayama T., Iwama H., Mayumi D., Yonebayashi H. (2021) Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections. Journal of Petroleum Science and Engineering, https://doi.org/10.1016/j.petrol.2021.108458. Suda, K., Gilbert, A., Yamada, K., Yoshida, Y., Ueno, Y. (2017) Compound- and position-specific carbon isotopic signatures of abiogenic hydrocarbons from on-land serpentinite-hosted Hakuba Happo hot spring in Japan. Geochimica et Cosmochimica Acta, 206, 201-215. Gilbert, A., Yamada, K., Suda, K., Ueno, Y., Yoshida, Y. (2016) Measurement of position－specific 13C isotopic composition of propane at the nanomole level. Geochimica et Cosmochimica Acta, 177, 205-216. Suda, K., Ueno, Y., Yoshizaki, M., Nakamura, H., Kurokawa, K., Nishiyama, E., Yoshino, K., Hongoh, Y, Kawachi, K, Omori, S., Yamada, K., Yoshida, N., Maruyama, S. (2014) Reply to comment on "Origin of methane in serpentinite－hosted hydrothermal systems: The CH4－H2－H2O hydrogen isotope systematics of the Hakuba Happo hot spring” by Suda et al. [Earth Planet. Sci. Lett. 386(2014) 112－125]. Earth and Planetary Science Letters 401, 376-377. Suda, K., Ueno, Y., Yoshizaki, M., Nakamura, H., Kurokawa, K., Nishiyama, E., Yoshino, K., Hongoh, Y, Kawachi, K, Omori, S., Yamada, K., Yoshida, N., Maruyama, S. (2014) Origin of methane in serpentinite－hosted hydrothermal systems: The CH4－H2－H2O hydrogen isotope systematics of the Hakuba Happo hot spring. Earth and Planetary Science Letters 386, 112－125.

Dr. Yusuke Miyajima

Research Fields Geochemistry, Organic geochemistry, Paleontology Research Topics Microbial methanogenesis and methane oxidation in gas hydrate fields Fluid and material cycling at modern and ancient submarine methane seeps Isotope geochemistry of methane-seep carbonate rocks Comment My research interest is the impact of subseafloor geochemical and geodynamic processes on biological activity. Publication Yusuke Miyajima, Ayaka Saito, Hiroyuki Kagi, Tatsunori Yokoyama, Yoshio Takahashi, Takafumi Hirata (2020) Incorporation of U, Pb, and rare earth elements in calcite through crystallization from amorphous calcium carbonate: simple preparation of reference materials for microanalysis. Geostandards and Geoanalytical Research, 45, 189-205. Krzysztof Hryniewicz, Yusuke Miyajima, Kazutaka Amano, Magdalena N. Georgieva, Michal Jakubowicz, Robert G. Jenkins, Andrzej Kaim (2020) Formation, diagenesis and fauna of cold seep carbonates from the Miocene Taishu Group of Tsushima (Japan). Geological Magazine, DOI: 10.1017/s001675682000103x. Miyajima, Y., Watanabe, Y., Goto, A.S., Jenkins, R.G., Sakai, S., Matsumoto, R., Hasegawa, T. (2020) Archaeal lipid biomarker as a tool to constrain the origin of methane at ancient methane seeps: Insight into subsurface fluid flow in the geological past. Journal of Asian Earth Sciences 189, 104134. Amano, K., Miyajima, Y., Jenkins, R.G., Kiel, S. (2019) The Miocene to Recent biogeographic history of vesicomyid bivalves in Japan, with two new records of the family. The Nautilus 133, 48-56. Amano, K., Miyajima, Y., Nakagawa, K., Hamuro, M., Hamuro, T. (2019) Chemosymbiotic bivalves from the lower Miocene Kurosedani Formation in Toyama Prefecture, central Honshu, Japan. Paleontological Research 23, 208-219. Miyajima, Y., Ijiri, A., Miyake, A., Hasegawa, T. (2018) Origin of methane and heavier hydrocarbons entrapped within Miocene methane-seep carbonates from central Japan. Chemical Geology 498, 83-95. 　　　　　　　　　　 Miyajima, Y., Watanabe, Y., Jenkins, R.G., Goto, A.S., Hasegawa, T. (2018) Diffusive methane seepage in ancient deposits: examples from the Neogene Shin’etsu sedimentary basin, central Japan. Journal of Sedimentary Research 88, 449-466. 　　　　　　　　　　 Miyajima, Y., Nobuhara, T., Koike, H. (2017) Taxonomic reexamination of three vesicomyid species (Bivalvia) from the middle Miocene Bessho Formation in Nagano Prefecture, central Japan., with notes on vesicomyid diversity. The Nautilus 131, 51-66. 　　　　　　　　　　 Hryniewicz, K., Bitner, M.A., Durska, E., Hagstrom, J., Hjalmarsdottir, H.R., Jenkins, R.G., Little, C.T.S., Miyajima, Y., Nakrem, H.A., Kaim, A. (2016) Paleocene methane seep and wood-fall marine environments from Spitsbergen, Svalbard. Palaeogeography, Palaeoclimatology, Palaeoecology 462, 41-56. 　　　　　　　　　　 Miyajima, Y., Watanabe, Y., Yanagisawa, Y., Amano, K., Hasegawa, T., Shimobayashi, N. (2016) A late Miocene methane-seep deposit bearing methane-trapping silica minerals at Joetsu, central Japan. Palaeogeography, Palaeoclimatology, Palaeoecology 455, 1-15.

Dr. Susumu Sakata

Research Fields Organic Geochemistry, Biogeochemistry Research Topics Distribution of geomicrobes assessed by lipid biomarkers Isotopic fractionation associated with microbial processes Origin of natural gas Comment My Motto：Constant dropping wears away a stone. Publication Katayama T., Yoshioka H., Kaneko M., Amo M., Fujii T., Takahashi H.A., Yoshida S., Sakata S. (2022) Cultivation and biogeochemical analyses reveal insights into methanogenesis in deep subseafloor sediment at a biogenic gas hydrate site. The ISME Journal, https://doi.org/10.1038/s41396-021-01175-7. Kurth J.M., Nobu M.K., Tamaki H., de Jonge N., Berger S., Jetten S.M., Yamamoto K., Mayumi D., Sakata S., Bai L., Cheng L., Nielsen J.L., Kamagata Y., Wagner T., Welte C.U. (2021) Methanogenic archaea use a bacteria-like methyltransferase system to demethoxylate aromatic compounds.The ISME Journal, 15, 3549–3565. https://doi.org/10.1038/s41396-021-01025-6. Suda K., Ikarashi M., Tamaki H., Tamazawa S., Sakata S., Maeda H., Kamagata Y., Kaneko M., Ujiie T., Shinotsuka Y., Wakayama T., Iwama H., Mayumi D., Yonebayashi H. (2021) Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections. Journal of Petroleum Science and Engineering, https://doi.org/10.1016/j.petrol.2021.108458. Sakamoto S., Nobu M.K., Mayumi D., Tamazawa S., Kusada H., Ikarashi M., Wakayama T., Iwama H., Yonebayashi H., Maeda H., Sakata S., Tamura T., Nomura N., Kamagata Y., Tamaki H. (2021) Koleobacter methoxysyntrophicus gen. nov., sp. nov., a novel anaerobic bacterium isolated from deep subsurface oil field and proposal of Koleobacteraceae fam. nov. and Koleobacterales ord. nov. within the class Clostridia of the phylum Firmicutes. Systematic and Applied Microbiology, https://doi.org/10.1016/j.syapm.2020.126154. Yoshioka H., Takeuchi M., Sakata S., Takahashi M., Tanabe S., Inamura A., Yasuhara M. (2020) Microbial methane production and oxidation in the Holocene mud beneath the Kanto Plain of central Japan. Geochemical Journal, Vol. 54 (No. 4), pp. 243-254. Aoyagi T., Inaba T., Aizawa H., Mayumi D., Sakata S., Charfi A., Suh C., Lee J., Sato Y., Ogata A., Habe H., Hori T. (2020) Unexpected diversity of acetate degraders in anaerobic membrane bioreactor treating organic solid waste revealed by high-sensitivity stable isotope probing. Water Research 176, 115750. Takeuchi Mio, Haruka Ozaki, Satoshi Hiraoka, Kamagata Yoichi, Sakata Susumu, Hideyoshi Yoshioka, Iwasaki Wataru (2019) Possible cross-feeding pathway between a methanotroph and a facultative methylotroph. PlosONE 14, e0213535. Katayama T., Yoshioka H., Yamanaka, T., Takeuchi, M., Muramoto Y., Usami J., Ikeda, H., Kamagata Y., Sakata S. (2019) Microbial community structure in deep natural gas-bearing aquifers subjected to sulfate-containing fluid injection. Journal of Bioscience and Bioengineering, 127, 45-51. Yuji Sano, Naoya Kinoshita, Takanori Kagoshima, Naoto Takahata, Susumu Sakata, Tomohiro Toki, Shinsuke Kawagucci, Amane Waseda, Tefang Lan, Hsinyi Wen, Ai-Ti Chen, Tsanyao F. Yang, Zheng Guodong, Yama Tomonaga, Emilie Roulleau and Daniele L. Pinti (2017) Origin of methane-rich natural gas at the West Pacific convergent plate boundary. Scientific Reports 67, Article number 15646. Tamazawa S., Mayumi D., Mochimaru H., Sakata S., Maeda H., Wakayama T., Ikarashi M., Kamagata Y., Tamaki H. (2017) Petrothermobacter organivorans gen. nov., sp. nov., a novel thermophilic, strictly anaerobic bacterium of the phylum Deferribacteres isolated from a deep subsurface oil reservoir. International Journal of Systematic and Evolutionary Microbiology 67, 3982-3986. Mayumi D., Mochimaru H., Tamaki H., Yamamoto K., Yoshjoka H., Suzuki Y., Kamagata Y., Sakata S.(2016) Methane production from coal by a single methanogen. Science354 (6309), 222-225. Mochimaru H., Tamaki H., Katayama T., Imachi H., Sakata S., Kamagata Y. (2016) Methanomicrobium antiquum sp. nov., a hydrogenotrophic methanogen isolated from deep sedimentary aquifers in a natural gas field. International Journal of Systematic and Evolutionary Microbiology 66, 4873-4877. Katayama T., Yoshioka H., Takahashi H.A., Amo M., Fujii T., Sakata S.(2016) Changes in microbial communities associated with gas hydrates in subseafloor sediments from the Nankai Trough. FEMS Microbiology Ecology 92, flw093. Yoshioka H, Mochimaru H, Sakata S. Takeda H, Yoshida S.(2015) Methane production potential of subsurface microbes in Pleistocene sediments from a natural gas field of the dissolved-in-water type, central Japan. Chemical Geology 419, 92-101. Katayama T., Yoshioka H., Muramoto Y., Usami J., Fujiwara K., Yoshida S., Kamagata Y., Sakata S. (2015) Physicochemical impacts associated with natural gas development on methanogenesis in deep sand aquifers. The ISME Journal 9, 436-446. Takeuchi M., Yamagishi T., Kamagata Y., Oshima K., Hattori M., Katayama T., Hanada S., Tamaki H., Marumo K., Maeda H., Nedachi M., Iwasaki W., Suwa Y., Sakata S. (2015) Tepidicaulis marinus gen. nov., sp. nov., a novel marine bacterium reducing nitrate to nitrous oxide strictly under microaerobic conditions. International Journal of Systematic and Evolutionary Microbiology, in press (doi: 10.1099/ijs.0.000167). Oba M., Sakata S., Fujii T. (2015) Archaeal polar lipids in deep marine sediments from the Nankai Trough. Archaeal polar lipids in subseafloor sediments from the Nankai Trough: Implications for the distribution of methanogens in the deep marine subsurface. Organic Geochemistry 78, 153-160. Takeuchi M., Kamagata Y., Oshima K., Hanada S., Tamaki H., Marumo K., Maeda H., Nedachi M., Hattori M., Iwasaki W., Sakata S. (2014) Methylocaldum marinum sp. nov., a thermotolerant, methane oxidizing bacterium isolated from marine sediments, and emended description of the genus Methylocaldum. International Journal of Systematic and Evolutionary Microbiology 64, 3240-3246.　 Komatsu　T., Kato S., Hirata K., Takashima R., Ogata Y., Oba M., Naruse H., Ta P.H., Nguyen P.D., Dang H.T., Doan T.N., Nguyen H.H., Sakata S., Kaiho K., Konigshof P. (2014) Devonian - Carboniferous transition containing a Hangenberg Black Shale equivalent in the Pho Han Formation on Cat Ba Island, northeastern Vietnam. Palaeogeography, Palaeoclimatology, Palaeoecology 404, 30-43. Katayama T., Yoshioka H., Mochimaru H., Meng X.Y., Muramoto Y., Usami J., Ikeda, H., Kamagata Y., Sakata S. (2014) Methanohalophilus levihalodurans sp. nov., a slightly halophilic, methylotrophic methanogen isolated from natural gas-bearing deep aquifers, and emended description of the genus Methanohalophilus. International Journal of Systematic and Evolutionary Microbiology 64, 2089-2093. Takeuchi M., Oshima K., Yamagishi T., Katayama T., Hanada S., Tamaki H., Kamagata Y., Marumo K., Nedachi M., Maeda H., Sakata S. (2014) Methyloceanibacter cenitepidus gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent. International Journal of Systematic and Evolutionary Microbiology 64, 462-468. Mayumi D., Dolfing J., Sakata S., Maeda H., Miyagawa Y., Ikarashi M., Tamaki H., Takeuchi M., Nakatsu C. H., and Kamagata Y. (2013) Carbon dioxide concentration dictates alternative methanogenic pathways in oil reservoirs. Nature Communications, DOI: 10.1038/ncomms2998. Sakata S., Maekawa T., Igari S., and Sano Y. (2012) Geochemistry and origin of natural gases dissolved in brines from gas fields in southwest Japan. Geofluids, 12, 327-335. Kobayashi　H., Endo　K., Sakata　S., Mayumi D., Kawaguchi H., Ikarashi M., Miyagawa Y., Maeda H., Sato K. (2012) Phylogenetic diversity of microbial communities associated with the crude-oil, large-insoluble-particle, and formation-water components of the reservoir fluid from a non-flooded high-temperature biodegraded petroleum reservoir. Journal of Bioscience and Bioengineering 113, 204-210. Kobayashi H., Kawaguchi H., Endo K., Mayumi D., Sakata S., Ikarashi M., Miyagawa Y., Maeda H., Sato K. (2012) Analysis of methane production by microorganisms indigenous to a depleted oil reservoir for application in Micobial Enchance Oil Recovery. Journal of Bioscience and Bioengineering 113, 84-87. Takeuchi　M., Yoshioka　H., Seo Y., Tanabe S., Tamaki H., Kamagata Y., Takahashi H. A., Igari S., Mayumi D., Sakata S. (2011) A distinct freshwater-adapted subgroup of ANME-1 dominates active archaeal communities in terrestrial subsurfaces in Japan. Environmental Microbiology 13, 3206-3218. Mayumi D., Mochimaru H., Yoshioka H., Sakata S., Maeda H., Miyagawa Y., Ikarashi M., Takeuchi M., Kamagata Y. (2011) Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field (Japan). Environmental Microbiology 13, 1995-2006. Yoshioka H., Maruyama A., Nakamura T., Higashi Y., Fuse H., Sakata S., Bartlett D. H. (2010) Activities and distribution of methanogenic and methane-oxidizing microbes in marine sediments from the Cascadia Margin. Geobiology 8, 223-233. Maeda H., Miyagawa Y., Ikarashi M., Mayumi D., Mochimaru H., Yoshioka H., Sakata S., Kobayashi H., Sato K. (2010) Research for Microbial Conversion of Residual Oil into Methane in Depleted Oil Fields to Develop New EOR Process. Proceedings of Abudabi Oil and Gas Conference and Exhibition 2010, Abudabi. OnePetro, No. 138135-MS. Yoshioka H., Sakata S., Cragg B., Parkes J., Fujii T. (2009) Microbial methane production rates in gas hydrate-bearing sediments from the eastern Nankai Trough, off central Japan. Geochemical Journal 43, 315-321. Takahashi S., Oba M., Kaiho K., Yamakita S., Sakata S. (2009) Panthalassic oceanic anoxia at the end of the Early Triassic: a cause of delay in the recovery of life after the end-Permian mass extinction. Palaeogeography, Palaeoclimatology, Palaeoecology 274, 185-195. Mochimaru H., Tamaki H., Hanada S., Imachi H., Nakamura K., Sakata S., Kamagata Y. (2009) Methanolobus profundi sp. nov., a new methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field. International Journal of Systematic and Evolutionary Microbiology 59, 714-718. Maeda H., Miyagawa Y., Ikarashi M., Kobayashi H., Sato K., Sakata S., Mochimaru H. (2009) Development of microbial conversion process of residual oil to methane in depleted oil fields. SPE 122573 Proceedings of Asia Pacific Oil and Gas Conference and Exhibition 2009, Jakarta. OnePetro, No. 122573-MS. Sakata S., Hayes J. M., Rohmer M., Hooper A., Seeman M. (2008) Stable carbon isotopic compositions of lipids isolated from an ammonia-oxidizing chemoautotroph. Organic Geochemistry 39, 1725-1734. Yoshioka H., Sakata S., Kamagata Y. (2008) Hydrogen isotope fractionation by Methanothermobacter thermoautotrophicus in coculture and pure culture conditions. Geochimica et Cosmochimica Acta 72, 2687-2694. Mochimaru H., Yoshioka H., Tamaki H., Nakamura K., Kaneko N., Sakata S., Imachi H., Sekiguchi Y., Uchiyama H., Kamagata Y. (2007) Microbial diversity and methanogenic potential in high temperature natural gas associated water in Japan. Extremophiles 11, 453-461. Oba M., Sakata, S., Tsunogai U. (2006) Polar and neutral isopranylglycerol ether lipids as biomarkers of archaea in near-surface sediments from the Nankai Trough. Organic Geochemistry 37, 1643-1654. Yamanaka T., Sakata S. (2004) Abundance and distribution of fatty acids in hydrothermal vent sediments of the western Pacific Ocean. Organic Geochemistry 35, 573-582. Sakata S., Sano Y., Maekawa T., Igari S. (1997) Hydrogen and carbon isotopic composition of methane as evidence for biogenic origin of natural gases from the Green Tuff Basin, Japan.  Organic Geochemistry 26, 399-407. Sakata S., Hayes J.M., McTaggart A.R., Evans R.A., Leckrone K.J., Togasaki R. K. (1997) Carbon isotopic fractionation associated with lipid biosynthesis by a cyanobacterium: Relevance for interpretation of biomarker records. Geochimica et Cosmochimica Acta 61, 5379-5389. Maekawa, T., Sakata, S., Igari, S., Imai, N. (1997) Organic geochemistry of the sources and generation environment for gas hydrate methane. Proceedings of the International Workshops on Gas Hydrate Studies. 328-339. Higashihara M., Igari S., Maekawa T., Noda T., Sakata S., Asada N., Miyatake, A. (1996)  C5+ hydrocarbons in fumarolic gases from the Kakkonda (Takinoue) geothermal area, Japan. Geochemical Journal 31, 63-73. Maekawa, T., Ito, S., Sakata S., Igari S., Imai N. (1995) Pressure and temperature conditions for methane hydrate dissociation in sodium chloride solutions. Geochemical Journal 29, 325-329. Sakata, S., Matsuhisa, Y., Igari, S. (1994) Organic geochemistry of petroleum from the Green Tuff basin, Japan. Organic Geochemistry 21, 27-34. Igari, S., Sakata, S. (1992) Fractionation of light hydrocarbons through GC columns packed with rocks and minerals - Implications for natural gas migration. Geochemical Journal 26, 37-43. Fukushima K., Kondo H., Sakata S. (1992) Geochemistry of hydroxy acids in sediments-I. Some freshwater and brackish water lakes in Japan. Organic Geochemistry 18, 913-922. Fukushima K., Uzaki M., Sakata, S. (1992) Geochemistry of hydroxy acids in sediments-II. Estuarine and coastal marine environments. Organic Geochemistry 18, 923-932. Sakata S. (1991) Carbon isotope geochemistry of natural gases from the Green Tuff Basin, Japan. Geochimica et Cosmochimica Acta 55, 1395-1405. Sakata,S., Takahashi, M., Igari, S., Suzuki, N. (1989) Origin of light hydrocarbons from volcanic rocks in the Green Tuff region of Northeast Japan. Chemical Geology 74, 241-248. Igari, S., Sakata, S. (1989) Origin of natural gas of dissolved-in water type in Japan inferred from chemical and isotopic compositions: Occurrence of dissolved gas of thermogenic origin.  Geochemical Journal 23, 139-142. Sakata, S., Akiyama, M. (1989) Steroid and hopanoid hydrocarbons in the Miocene formations from the MITI-Hamayuchi borehole in northern Hokkaido, Japan. Geochemical Journal 23, 237-244. Sakata S., Suzuki N., Kaneko N. (1988) A biomarker study of petroleum from the Neogene Tertiary sedimentary basins in Northeast Japan. Geochemical Journal 22, 89-105. Igari, S., Sakata, S. (1988) Chemical and isotopic compositions of natural gases from the Japanese major oil and gas fields: Origin and compositional change due to migration. Geochemical Journal 22, 257-263.

page top▲