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Development of an ultra-high sensitive and high-speed glycomic profiling scanner

In this study, an ultrahigh sensitive and high-speed glycomic profiling scanner was developed by research groups, including Patcharaporn Boottanun (AIST Postdoctoral Researcher), Chiaki Nagai-Okatani (Senior Researcher) and Atsushi Kuno (Group Leader) of Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, AIST, and Dr. Masao Yamada of EMUKK LLC, etc.

AIST has been involved in the development of a glycomic profiling scanner based on an evanescent-field fluorescence detection principle [1]. In this study, a new lectin microarray scanner (GSR2300) was demonstrated to allow high-speed (~15 sec) analysis with improved sensitivity, precision, and reproducibility, compared with an old version (GSR1200). Owing to a 10-fold higher sensitive detection system, the new GSR2300 enables 4-fold higher sensitive analysis even with 10-fold shorter scan time than the old GSR1200. Using the GSR2300, only quite a small amount of glycoprotein sample is required, such as 3 cultured cells and FFPE tissue fragments (0.1 mm2 of 5 μm thick section; ~60 cells equivalent of volume). Accordingly, this novel glycomic profiling system allows for comparing glycomic features of cell subpopulations in tiny areas on tissue sections, and thus facilitates accelerating the development of “glycomedicine” utilizing disease-related glycan alterations.

The lectin microarray scanner GSR2300 is now available at EMUKK LLC [2].

Glycomic profiling analysis of glycoproteins (A, B) and tissue sections (C) using the new (GSR2300) and old (GSR1200) scanners

Glycomic profiling analysis


Emukk LLC


  • Title:An improved evanescent fluorescence scanner suitable for high-resolution glycome mapping of formalin-fixed paraffin-embedded tissue sections
  • Authors:Patcharaporn Boottanun, Chiaki Nagai-Okatani, Misugi Nagai, Umbhorn Ungkulpasvich, Shinjiro Yamane, Masao Yamada, Atsushi Kuno.
  • Journal:Analytical and Bioanalytical Chemistry (published online: July 3, 2023)


  1. Development of a Precise Sugar-Chain Profiling Scanner Using a Lectin Arraying Technique (AIST)
  2. Glycan Profiler GlycoStation Reader 2300 (GSR2300) is able to take its glycan profile from only three cells (emukk LLC)


This study was funded by a project for utilizing glycans in the development of innovative drug discovery technologies (grant number JP20ae0101021h0005) from the Japan Agency for Medical Research and Development (AMED).

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