Multicellular System Regulation Research Group

Research Project

Glycans cover the outermost layer of all cells and change depending on the cell type and condition (differentiation, cancer, etc.), thus they are called the “cell signature“. They are expected to be used for quality control of cells used in regenerative medicine and as drug targets for cancer and other diseases. We have constructed a recombinant lectin library and have been developing a technology for profiling glycans using this library. We have developed and used lectin arrays in which multiple lectins are immobilized on glass slides. Recently, we have also developed a glycan profiling by sequencing, and succeeded in establishing a technology (scGR-seq) for simultaneous analysis of glycan expression and gene expression in individual cells. We have also applied this technology to glycan profiling of microbiota. We will continue to further advance and apply this technology.

Research Project

Human pluripotent cells and human mesenchymal stem cells are expected as cell sources for regenerative medicine. When transplanting these cells into humans, ensuring the safety and quality of the cells is extremely important. So far, we have succeeded in developing a technology for non-destructively detecting and removing the tumorigenic human pluripotent stem cells remaining in the cells for transplantation, and have successfully commercialized it in collaboration with a company. We will continue to develop new quality control technology.

Research Project

The development of diagnostic techniques for cancer, diabetes, and psychiatric/neurological disorders is important for definitive diagnosis, decision of treatment policy, improvement of QOL, and early rehabilitation. We aim to develop a new diagnostic technology by analyzing the cell function and gene/glycan expression of diseased cells (prostate cancer, breast cancer, pancreatic cancer, diabetes, mental disorders).

Research Project

Exosomes are membrane-bound extracellular vesicles (EVs) produced in the endosomal compartment of most eukaryotic cells that are transported to other cells by the systemic circulation, and function as an intercellular communication tool in Embryological, Physiological, and Pathological processes. We collaborate with researchers inside and outside the laboratory to advance the technology for preparing EVs that are in an intrinsic state immediately after they are secreted from cells, develop technology for their utilization, and understand the functions of glycans of EVs. We will try to clarify the essence of the mysterious exosome.

Research Project

Microwaves are widely used as an efficient heating method for chemical reactions, but have not been applied to fine chemical syntheses, which require reactions at low temperatures or under precise temperature control. All chemists admit heating properties of microwaves but some are suspicious of the unique effects of microwaves, even deriding them as a 'hoax'.
We have 1) constructed a microwave-irradiated reactor equipped with a cooling unit allowing precise temperature control and irradiation at low temperatures, 2) adapted it for oligosaccharide and peptide syntheses and clarified the unique effects of microwaves. Then, we successfully 3) achieved to improve both stability and reactivity for expensive and valuable glyco-amino acid synthons and established synthesis methods of complex glycopeptides in tens of milligram scale, 4) considered the unique effect of microwaves as molecular selective activation in an enzymatic reaction.

Results

• Hayashi, Y; Tajiri, K; Ozawa, T; Angata, K; Sato, T; Togayachi, A; Nagashima, I; Shimizu, H; Murayama, A; Muraishi, N; Narimatsu, H; Yasuda, I.
  Impact of preS1 Evaluation in the Management of Chronic Hepatitis B Virus Infection.
  MEDICINA (Kaunas). 2024 Aug 16;60(8):1334. doi: 10.3390/medicina60081334
• Satoh, T; Yagi-Utsumi, M; Ishii, N; Mizushima, T; Yagi, H; Kato, R; Tateno, H; Matsuo, I; Kato, K; Suzuki, T; Yoshida, Y.
  Structural basis of sugar recognition by SCFFBS2 ubiquitin ligase involved in NGLY1 deficiency.
  FEBS LETTERS. 2024 Aug 22. doi: 10.1002/1873-3468.15003
　　　　　　　　　　　　　　　　　　　
• Páez-Watson, T; Tomás-Martínez, S; de, Wit R; Keisham, S; Tateno, H; van, Loosdrecht MCM; Lin, Y.
  Sweet Secrets: Exploring Novel Glycans and Glycoconjugates in the Extracellular Polymeric Substances of “Candidatus Accumulibacter”.
  ACS ES T WATER. 2024 Jul 12;4(8):3391-3399. doi: 10.1021/acsestwater.4c00247
• Yamasaki, K; Tsuzuki, S; Tateno, H.
  Stabilization of the Protein Structure by the Many-Body Cooperative Effect in the NH/π Hydrogen-bonding Tryptophan Triad.
  J PHYS CHEM B. 2024 Jul 17. doi: 10.1021/acs.jpcb.4c02391
• Arai, J; Hayakawa, Y; Tateno, H; Fujiwara, H; Kasuga, M; Fujishiro, M.
  The role of gastric mucins and mucin-related glycans in gastric cancers.
  CANCER SCI. 2024 Jul 19. doi: 10.1111/cas.16282
• Wang, ZG; Yang, ZH; Shishido, M; Daoudi, K; Hidaka, M; Tateno, H; Futai, E; Ogawa, T.
  Microcystis viridis NIES-102 Cyanobacteria Lectin (MVL) Interacts with SARS-CoV-2 Spike Protein Receptor Binding Domains (RBDs) via Protein-Protein Interaction.
  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2024 Jun 18;25(12):6696. doi: 10.3390/ijms25126696
• Nie, H; Saini, P; Miyamoto, T; Liao, L; Zielinski, RJ; Liu, H; Zhou, W; Wang, C; Murphy, B; Towers, M; Yang, T; Qi, Y; Kannan, T; Kossenkov, A; Tateno, H; Claiborne, DT; Zhang, N; Abdel-Mohsen, M; Zhang, R.
  Targeting branched N-glycans and fucosylation sensitizes ovarian tumors to immune checkpoint blockade.
  NAT COMMUN. 2024 Apr 2;15(1):2853. doi: 10.1038/s41467-024-47069-y
• Arai, J; Hayakawa, Y; Tateno, H; Murakami, K; Hayashi, T; Hata, M; Matsushita, Y; Kinoshita, H; Abe, S; Kurokawa, K; Oya, Y; Tsuboi, M; Ihara, S; Niikura, R; Suzuki, N; Iwata, Y; Shiokawa, T; Shiomi, C; Uekura, C; Yamamoto, K; Fujiwara, H; Kawamura, S; Nakagawa, H; Mizuno, S; Kudo, T; Takahashi, S; Ushiku, T; Hirata, Y; Fujii, C; Nakayama, J; Shibata, S; Woods, S; Worthley, DL; Hatakeyama, M; Wang, TC; Fujishiro, M.
  Impaired glycosylation of gastric mucins drives gastric tumorigenesis and serves as a novel therapeutic target.
  GASTROENTEROLOGY. 2024 Apr 5:S0016-5085(24)00363-9. doi: 10.1053/j.gastro.2024.03.037
• Miura, I; Shams, F; Ohki, J; Tagami, M; Fujita, H; Kuwana, C; Nanba, C; Matsuo, T Ogata, M; Mawaribuchi, S; Shimizu, N; Ezaz, T.
  Multiple Transitions between Y Chromosome and Autosome in Tagos Brown Frog Species Complex.
  GENES (Basel). 2024 Feb 26;15(3):300. doi: 10.3390/genes15030300
• Nakahashi, H; Oda, T; Shimomura, O; Akashi, Y; Takahashi, K; Miyazaki, Y; Furuta, T; Kuroda, Y; Louphrasitthiphol, P; Mathis, BJ; Tateno, H.
  Aberrant Glycosylation in Pancreatic Ductal Adenocarcinoma 3D Organoids Is Mediated by KRAS Mutations.
  J ONCOL. 2024 Mar 18;2024:1529449. doi: 10.1155/2024/1529449
• Nagashima, I; Shimizu, H.
  Preparation of Mucin Glycopeptides by Organic Synthesis.
  METHODS MOL BIOL. 2024;2763:187-199. doi: 10.1007/978-1-0716-3670-1_16
• Chen, LM; Keisham, S; Tateno, H; van, Ede J; Pronk, M; van, Loosdrecht MCM; Lin, Y.
  Alterations of Glycan Composition in Aerobic Granular Sludge during the Adaptation to Seawater Conditions.
  ACS ES T WATER. 2023 Dec 27;4(1):279-286. doi: 10.1021/acsestwater.3c00625
• Keisham, S; Saito, S; Kowashi, S; Tateno, H.
  Droplet-Based Glycan and RNA Sequencing for Profiling the Distinct Cellular Glyco-States in Single Cells.
  SMALL METHODS. 22024 Jan 2:e2301338. doi: 10.1002/smtd.202301338
• Yonezawa, A; Mizushima, K; Hirai, Y; Tateno, H; Muto, A; Igarashi, K; Takag, T; Naito, Y; Higashimura, Y.
  Involvement of transcription repressor Bach1 in function of intestinal goblet cells.
  ANNALS OF NUTRITION AND METABOLISM. 2023 ;79 (suppl 1) 1170-1170; AUG. doi: 10.1159/000530786
• Ishii, N; Noguchi, K; Ikemoto, MJ; Yohda, M; Odahara, T.
  Optimizing Exosome Preparation Based on Size and Morphology: Insights From Electron Microscopy.
  MICROSC MICROANAL. 2023 Oct 13:ozad103. doi: 10.1093/micmic/ozad103
• Ishii, N; Odahara, T.
  Investigation of the Efficacy of Lanthanoid Heavy Metal Acetates as Electron Staining Reagents for Biomembrane Vesicles.
  MICROSC MICROANAL. 2023 Oct 13:ozad107. doi: 10.1093/micmic/ozad107
• Odaka, H.
  Target-Specific O-linked N-acetylglucosamine Modification with a Dual-Specificity Aptamer Reveals Functions of O-GlcNAcylation in beta-catenin.
  TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY. May 25, 2023; Volume 35(2023) Issue 205. doi: 10.4052/tigg.2309.6E
• Watanabe, T, Tateno, H.
  Lectin Blotting of Cell Lysates.
  CURR PROTOC. 2023 May;3(5):e790. doi: 10.1002/cpz1.790
• Kuroda, Y; Oda, T; Shimomura, O; Louphrasitthiphol, P; Mathis, BJ; Tateno, H, Hatano, K.
  Novel positron emission tomography imaging targeting cell surface glycans for pancreatic cancer: (18) F-labeled rBC2LCN lectin.
  CANCER SCI. 2023 May 19. doi: 10.1111/cas.15846
• Odaka, H; Tateno, H.
  Single-Cell Glycan and RNA Sequencing (scGR-seq).
  CURR PROTOC. 2023 May;3(5):e777. doi: 10.1002/cpz1.777
• Ikemoto, MJ; Aihara, Y; Ishii, N; Shigemori, H.
  3,4-Dihydroxybenzalacetone Inhibits the Propagation of Hydrogen Peroxide-Induced Oxidative Effect via Secretory Components from SH-SY5Y Cells.
  BIOL PHARM BULL. 2023;46(4):599-607. doi: 10.1248/bpb.b22-00727
• Murata, Y; Jitsukawa, T; Iida, M; Furuta, T; Haramoto, Y; Shigeri, Y; Fujisaki, S.
  Liquid chromatography-mass spectrometry analyses of polyprenyl phosphates in Escherichia coli cells in which genes for isoprenoid synthesis are amplified.
  J BIOSCI BIOENG. 2023 Mar 1:S1389-1723(23)00047-6. doi: 10.1016/j.jbiosc.2023.02.003
• Mawaribuchi, S; Ito, M; Ogata, M; Yoshimura, Y; Miura, I.
  Parallel Evolution of Sex-Linked Genes across XX/XY and ZZ/ZW Sex Chromosome Systems in the Frog Glandirana rugosa.
  GENES (Basel). 2023 Jan 18;14(2):257. doi: 10.3390/genes14020257
• Sato, Y; Kato, Y; Iizumi, Y; Morimoto, T; Kobashi, K; Sugino, T; Tateno, H; Okazaki, T.
  Size distributions of cellulose nanocrystals in dispersions using the centrifugal sedimentation method.
  INT J BIOL MACROMOL. 2023 Feb 2:123520. doi: 10.1016/j.ijbiomac.2023.123520
• Mawaribuchi, S; Shimomura, O; Oda, T; Hiemori, K; Shimizu, K; Yamase, K; Date, M; Tateno, H.
  rBC2LCN-reactive SERPINA3 is a glycobiomarker candidate for pancreatic ductal adenocarcinoma.
  GLYCOBIOLOGY. 2023 Feb 2:cwad009. doi: 10.1093/glycob/cwad009
• Huang, T; Sato, Y; Kuramochi, A; Ohba, Y; Sano, M; Miyagishi, M; Tateno, H; Wadhwa, R; Kawasaki, K; Uchida, T; Ekdahl, KN; Nilsson, B; Chung, UI; Teramura, Y.
  Surface modulation of extracellular vesicles with cell-penetrating peptide-conjugated lipids for improvement of intracellular delivery to endothelial cells.
  REGEN THER. 2023 Jan 11;22:90-98. doi: 10.1016/j.reth.2022.12.007
• Mawaribuchi, S; Haramoto, Y; Ikeda, N; Ito, M.
  Evolutionary features of ligands and their receptors via protein-protein interactions and essentiality in primates.
  GENES CELLS. 2023 Jan 9. doi: 10.1111/gtc.13006
• Ishii, N.
  C-shaped dipper: A novel useful auxiliary tool for preparation of specimen grids for transmission electron microscopy.
  MICROSC RES TECH. 2022 Dec 29. doi: 10.1002/jemt.24283
• Ando, M; Kawasaki, H; Tamura, S; Haramoto, Y; Shigeri, Y.
  Recent Advances in Gas Sensing Technology Using Non-Oxide II-VI Semiconductors CdS, CdSe, and CdTe.
  CHEMOSENSORS. 2022 10(11)482. doi: 10.3390/chemosensors10110482
• Nagashima, I; Sugiyama, JI; Shimizu, H.
  Study of 400 MHz microwave conduction loss effect for a hydrolysis reaction by thermostable β-Glucosidase HT1.
  BIOSCI BIOTECHNOL BIOCHEM. 2022 Dec 6:zbac194. doi: 10.1093/bbb/zbac194
• Kuroda, Y; Shimomura, O; Nakahashi, H; Miyazaki, Y; Nakajima, T; Tateno, H; Oda, T.
  Lectin-based photoimmunotherapy targeting cell surface glycans for pancreatic cancer.
  CANCER RESEARCH 82 (22). 2022 NOV 15. doi: 10.1158/1538-7445.PANCA22-B083
• Kuroda, Y; Oda, T; Shimomura, O; Hashimoto, S; Akashi, Y; Miyazaki, Y; Furuya, K; Furuta, T; Nakahashi, H; Louphrasitthiphol, P; Mathis, BJ; Nakajima,T; Tateno, H.
  Lectin-based phototherapy targeting cell surface glycans for pancreatic cancer.
  INT J CANCER. 2022 Nov 22. doi: 10.1002/ijc.34362
• Tasaki, M; Tateno, H; Sato, T; Narimatsu, H; Saito, K; Nakagawa, Y; Aoki, T; Kamimura, M; Ushiki, T; Takahashi, K; Tomita, Y.
  Hyporesponsiveness against donors ABO antigens of renal grafts after ABO-incompatible kidney transplantation.
  CLIN EXP NEPHROL. 2022 Oct 8. doi: 10.1007/s10157-022-02280-3
• Kosaka, T; Yamaguchi, S; Izuta, S; Yamahira, S; Shibasaki, Y; Tateno, H; Okamoto, A.
  Bioorthogonal Photoreactive Surfaces for Single-Cell Analysis of Intercellular Communications.
  AM CHEM SOC. 2022 Sep 20. doi: 10.1021/jacs.2c07321
• Yamasaki, K; Adachi, N; Ngwe Tun, MM; Ikeda, A; Moriya, T; Kawasaki, M; Yamasaki, T; Kubota, T; Nagashima, I; Shimizu, H; Tateno, H; Morita, K.
  Core fucose-specific Pholiota squarrosa lectin (PhoSL) as a potent broad-spectrum inhibitor of SARS-CoV-2 infection.
  FEBS J. 2022 Aug 25. doi: 10.1111/febs.16599
• Katsumi, T; Shams, F; Yanagi, H; Ohnishi, T; Toda, M; Lin, SM; Mawaribuchi, S; Shimizu, N; Ezaz, T; Miura, I.
  Highly rapid and diverse sex chromosome evolution in the Odorrana frog species complex.
  DEV GROWTH DIFFER. 2022 Jul 26. doi: 10.1111/dgd.12800
• Numakawa, T; Odaka, H.
  The Role of Neurotrophin Signaling in Age-Related Cognitive Decline and Cognitive Diseases.
  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022 Jul 13;23(14):7726. doi: 10.3390/ijms23147726
• Kokubu, R; Ohno, S; Kuratani, H; Takahashi, Y; Manabe, N; Shimizu, H; Chiba, Y; Denda-Nagai, K; Tsuiji, M; Irimura, T; Yamaguchi, Y.
  O-Glycan-Dependent Interaction between MUC1 Glycopeptide and MY.1E12 Antibody by NMR, Molecular Dynamics and Docking Simulations.
  INT J MOL SCI. 2022 Jul 16;23(14):7855. doi: 10.3390/ijms23147855
• Nagae, M; Hirata, T; Tateno, H; Mishra, SK; Manabe, N; Osada, N; Tokoro, Y; Yamaguchi, Y; Doerksen, RJ; Shimizu, T; Kizuka, Y.
  Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A).
  COMMUN BIOL. 2022 Jul 19;5(1):695. doi: 10.1038/s42003-022-03661-w
• Odaka, H.
  Genome-wide CRISPR/Cas9 Screening Identifies KDM2B as a Modifier of Heparan Sulfate-Protein Interactions.
  TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY. 2022 Volume 34 Issue 199 Pages E63. doi: 10.4052/tigg.2203.6E
• Tsukano, K; Yamamoto, T; Watanabe, T; Michiue, T.
  Xenopus Dusp6 modulates FGF signaling to precisely pattern pre-placodal ectoderm.
  DEVELOPMENTAL BIOLOGY. 2022 May 16. doi: 10.1016/j.ydbio.2022.05.009
• Oinam, L; Tateno, H.
  Glycan Profiling by Sequencing to Uncover Multicellular Communication: Launching Glycobiology in Single Cells and Microbiomes.
  FRONT CELL DEV BIOL. 2022 May 27;10:919168. doi: 10.3389/fcell.2022.919168
• Yang, Y; Akashi, Y; Shimomura, O; Tateno, H; Saito, S; Hiemori, K; Miyazaki, Y; Furuta, T; Kitaguchi, D; Kuroda, Y; Pakavarin, L; Oda, T.
  Glycan expression profile of signet ring cell gastric cancer cells and potential applicability of rBC2LCN-targeted lectin drug conjugate therapy.
  GASTRIC CANCER. 2022 Jun 17. doi: 10.1007/s10120-022-01312-x
• Miura, I; Shams, F; Jeffries, DL; Katsura, Y; Mawaribuchi, S; Perrin, N; Ito, M; Ogata, M; Ezaz, T.
  Identification of ancestral sex chromosomes in the frog Glandirana rugosa bearing XX-XY and ZZ-ZW sex-determining systems.
  MOL ECOL. 2022 Jun 12. doi: 10.1111/mec.16551
• Odaka, H; Hiemori, K; Shimoda, A; Akiyoshi, K; Tateno, H.
  CD63-positive extracellular vesicles are potential diagnostic biomarkers of pancreatic ductal adenocarcinoma.
  BMC GASTROENTEROLOGY. 2022 Mar 28;22(1):153. doi: 10.1186/s12876-022-02228-7
• Hayashi, Y; Yimiti, D; Sanada, Y; Ding, C; Omoto, T; Ogura, T; Nakasa, T; Ishikawa, M; Hiemori, K; Tateno, H; Miyaki, S; Adachi, N.
  The therapeutic capacity of bone marrow MSC-derived extracellular vesicles in Achilles tendon healing is passage-dependent and indicated by specific glycans.
  FEBS LETTERS. 2022 Apr;596(8):1047-1058. doi: 10.1002/1873-3468.14333
• Tasaki, M; Tateno, H; Sato, T; Tomioka, A; Kaji, H; Narimatsu, H; Saito, K; Nakagawa, Y; Aoki, T; Kamimura, M; Ushiki, T; Okada, M; Miwa, Y; Hotta, K; Yoshida, Y; Takahashi, K; Tomita, Y.
  A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation.
  TRANSPL INT. 2022 Mar 23;35:10248. doi: 10.3389/ti.2022.10248
• Oinam, L; Hayashi, R; Hiemori, K; Kiyoi, K; Sage-Ono, K; Miura, K; Ono, M; Tateno, H.
  Quantitative evaluation of glycan-binding specificity of recombinant Concanavalin A produced in lettuce (Lactuca sativa).
  BIOTECHNOL BIOENG. 2022 Apr 8. doi: 10.1002/bit.28099
• Ishii, N.
  Systematic Investigation of Lanthanoid Transition Heavy Metal Acetates as Electron Staining Reagents for Protein Molecules in Biological Transmission Electron Microscopy.
  MICROSC MICROANAL. 2022 Apr 1:1-10. doi: 10.1017/S1431927622000411
• Seo, N; Nakamura, J; Kaneda, T; Tateno, H; Shimoda, A; Ichiki, T; Furukawa, K; Hirabayashi, J; Akiyoshi, K; Shiku, H.
  Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method.
  J EXTRACELL VESICLES. 2022 Mar;11(3):e12205. doi: 10.1002/jev2.12205
• Odaka, H; Ozaki, H; Tateno, H.
  scGR-seq: Integrated analysis of glycan and RNA in single cells.
  STAR PROTOC. 2022 Feb 18;3(1):101179. doi: 10.1016/j.xpro.2022.101179. eCollection
• Irumagawa, S; Hiemori, K; Saito, S; Tateno, H; Arai, R.
  Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans.
  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23 (2);doi: 10.3390/ijms23020676 JAN
• Oinam, L; Tateno, H.
  Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System.
  METHODS MOL BIOL. 2022;2460:25-32. doi: 10.1007/978-1-0716-2148-6_2
• Kaifu, T; Yabe, R; Maruhashi, T; Chung, SH; Tateno, H; Fujikado, N; Hirabayashi, J; Iwakura, Y.
  DCIR and its ligand asialo-biantennary N-glycan regulate DC function and osteoclastogenesis.
  J EXP MED. 2021 Dec 6;218(12):e20210435. doi: 10.1084/jem.20210435. Epub 2021 Nov
• Shimoda, A; Miura, R; Tateno, H; Seo, N; Shiku, H; Sawada, S; Sasaki, Y; Akiyoshi, K.
  Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications.
  SMALL METHODS; doi: 10.1002/smtd.202100785
• Tulin, EKC; Nakazawa, C; Nakamura, T; Saito, S; Ohzono, N; Hiemori, K; Nakakita, SI; Tateno, H; Tonozuka, T; Nishikawa, A.
  Glycan detecting tools developed from the Clostridium botulinum whole hemagglutinin complex.
  SCI REP. 2021 Nov 9;11(1):21973. doi: 10.1038/s41598-021-01501-1
• Kaigawa, H; Tateno, H; Arai, R.
  Self-Assembling Functional Complexes of Protein Nanobuilding Blocks with a Trimeric Lectin.
  PROTEIN SCIENCE 30 158-159; OCT
• Irumagawa, S; Tateno, H; Arai, R.
  Self-Assembling Lectin Nano-Building Blocks Enhance Avidity to Glycans through Polyvalent Effect.
  PROTEIN SCIENCE 30 173-174; OCT
• Hoshi, K; Ito, H; Abe, E; Fuwa, TJ; Kanno, M; Murakami, Y; Abe, M; Murakami, T, Yoshihara, A, Ugawa, Y, Saito, T, Saido, TC, Matsumoto, K, Yamaguchi, Y, Furukawa, K, Arai, H, Kanai, M, Miyajima, M, Arai, H, Ogawa, N, Akatsu, H, Hashizume, Y, Tateno, H, Honda, T, Hashimoto, Y.
  Transferrin Biosynthesized in the Brain Is a Novel Biomarker for Alzheimers Disease.
  METABOLITES. 2021 Sep 10;11(9):616. doi: 10.3390/metabo11090616
• Minoshima, F; Ozaki, H; Odaka, H; Tateno, H.
  Integrated analysis of glycan and RNA in single cells.
  ISCIENCE. 2021 Jul 17;24(8):102882. doi: 10.1016/j.isci.2021.102882. eCollection
• Watanabe, T; Takeda, K; Hiemori, K; Minamisawa, T; Tateno, H.
  A glycosaminoglycan microarray identifies the binding of SARS-CoV-2 spike protein to chondroitin sulfate E.
  FEBS LETT. 2021 Aug 10. doi: 10.1002/1873-3468.14173
• Haramoto, Y.
  First person - Yoshikazu Haramoto.
  BIOLOGY OPEN 10 (4);doi: 10.1242/bio.058759 APR
• Ogita, Y; Tamura, K; Mawaribuchi, S; Takamatsu, N; Ito, M.
  Independent pseudogenizations and losses of sox15 during amniote diversification following asymmetric ohnolog evolution.
  BMC ECOL EVOL. 2021 Jun 30;21(1):134. doi: 10.1186/s12862-021-01864-z
• Furuta, T; Oda, T; Kiyoi, K; Ozawa, Y; Kimura, S; Kurimori, K; Miyazaki, Y; Yu, Y; Furuya, K; Akashi, Y; Shimomura, O; Tateno, H
  Carcinoembryonic antigen as a specific glycoprotein ligand of rBC2LCN lectin on pancreatic ductal adenocarcinoma cells
  Cancer Sci. 2021 Jun 11. doi: 10.1111/cas.15023
• Numakawa, T; Odaka, H.
  Brain-Derived Neurotrophic Factor Signaling in the Pathophysiology of Alzheimers Disease: Beneficial Effects of Flavonoids for Neuroprotection.
  INT J MOL SCI. 2021 May 27;22(11):5719. doi: 10.3390/ijms22115719
• Haramoto, Y; Sakata, M; Kobayashi, S.
  Visualization of X chromosome reactivation in mouse primordial germ cells in vivo.
  BIOL OPEN. 2021 Apr 15;10(4):bio058602. doi: 10.1242/bio.058602. Epub 2021 Apr 29
• Watanabe, T; Tateno, H.
  Elimination of cells deviated from human induced pluripotent stem cells with a photoactivatable IR700-labelled antibody.
  BIOCHEM BIOPHYS RES COMMUN. 2021 Mar 25;554:13-18. doi: 10.1016/j.bbrc.2021.03.078
• Furuta T, Tateno H, Kiyoi K, Miyazaki Y, Kimura S, Kurimori K, Shimomura O, Oda T
  Glycans on CEA as a targeting ligand of pancreatic ductal adenocarcinoma in novel lectin-drug conjugated therapy.
  CANCER SCIENCE 112 718-718; FEB
• Odaka H, Numakawa T, Soga M, Kido J, Matsumoto S, Kajihara R, Okumiya T, Tani N, Tanoue Y, Fukuda T, Furuya H, Inoue T, Era T
  An iPSC-based neural model of sialidosis uncovers glycolytic impairment-causing presynaptic dysfunction and deregulation of Ca(2+) dynamics
  Neurobiol Dis. 2021 Jan 28:105279. doi: 10.1016/j.nbd.2021.105279
• Kimura S, Oda T, Shimomura O, Enomoto T, Hashimoto S, Kuroda Y, Yu Y, Kurimori K, Furuta T, Miyazaki Y, Tateno H
  Novel Pancreatic Cancer Therapy Targeting Cell Surface Glycans by Liposomes Modified with rBC2LCN Lectin
  Eur Surg Res. 2021 Jan 27:1-10. doi: 10.1159/000513430
• Inagaki H, Haramoto Y, Kubota HY, Shigeri Y
  Complete mitochondrial genome sequence of Japanese forest green tree frog (Rhacophorus arboreus.
  Mitochondrial DNA B Resour 2020 Sep 16;5(3):3347-3348. doi: 10.1080/23802359.2020.1820396
• Odaka H, Hiemori K, Shimoda A, Akiyoshi K, Tateno H
  Platelet-derived extracellular vesicles are increased in sera of Alzheimers disease patients, as revealed by Tim4-based assays.
  FEBS OPEN BIO 2020 Dec 20. doi: 10.1002/2211-5463.13068
• Hasehira K, Furuta T, Shimomura O, Asada M, Oda T, Tateno H
  Quantitative structural analysis of glycans expressed within tumors derived from pancreatic cancer patient-derived xenograft mouse models
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• Kitaguchi D, Oda T, Enomoto T, Ohara Y, Owada Y, Akashi Y, Furuta T, Yu Y, Kimura S, Kuroda Y, Kurimori K, Miyazaki Y, Furuya K, Shimomura O, Tateno H
  Lectin Drug Conjugate Therapy for Colorectal Cancer
  Cancer Sci. 2020 Oct 15. (2020) doi: 10.1111/cas.14687
• Inagaki H, Haramoto Y, Kubota HY, Shigeri Y
  Complete mitochondrial genome sequence of Japanese forest green tree frog (Rhacophorus arboreus)
  MITOCHONDRIAL DNA PART B-RESOURCES 5 (3) 3365-3366; (2020) doi:10.1080/23802359.2020.1820396
• Watanabe T, Kakuta J, Saito S, Hasehira K, Kiyoi K, Imai T, Tateno H
  Monoclonal antibodies specific for podocalyxin expressed on human induced pluripotent stem cells.
  Biochem Biophys Res Commun. 2020 Sep 7:S0006-291X(20)31692-2. (2020) doi:10.1016/j.bbrc.2020.08.092
• Giron LB, Colomb F, Papasavvas E, Azzoni L, Yin X, Fair M, Anzurez A, Damra M, Mounzer K, Kostman JR, Tebas P, ODoherty U, Tateno H, Liu Q, Betts MR, Montaner LJ, Abdel-Mohsen M
  Interferon-α alters host glycosylation machinery during treated HIV infection
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• Wang J, Bhargava P, Yu Y, Sari AN, Zhang H, Ishii N, Yan K, Zhang Z, Ishida Y, Terao K, Kaul SC, Miyako E, Wadhwa R
  Novel Caffeic Acid Phenethyl Ester-Mortalin Antibody Nanoparticles Offer Enhanced Selective Cytotoxicity to Cancer Cells
  Cancers (Basel). 12(9):E2370. (2020) doi: 10.3390/cancers12092370
• Watanabe T, Saito S, Hiemori K, Kiyoi K, Mawaribuchi S, Haramoto Y, Tateno H
  SSEA-1-positive fibronectin is secreted by cells deviated from the undifferentiated state of human induced pluripotent stem cells
  Biochem Biophys Res Commun. 529 (3) 575-581;(2020) doi:10.1016/j.bbrc.2020.06.074
• Colomb F, Giron LB, Kuri-Cervantes L, Adeniji OS, Ma T, Dweep H, Battivelli E, Verdin E, Palmer CS, Tateno H, Kossenkov AV, Roan NR, Betts MR, Abdel-Mohsen M
  Sialyl-Lewis(X) Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy
  Cell Rep. Aug 4;32(5):107991. (2020) doi: 10.1016/j.celrep.2020.107991
• Oinam L, Changarathil G, Raja E, Ngo YX, Tateno H, Sada A, Yanagisawa H
  Glycome profiling by lectin microarray reveals dynamic glycan alterations during epidermal stem cell aging
  Aging Cell. 35: 0 (2020) doi:10.1111/acel.13190
• Haramoto Y^＃, Onuma Y^＃, Mawaribuchi S, Nakajima Y, Aiki Y, Higuchi K, Shimizu M, Tateno H, Hirabayashi J, Ito Y.
  A technique for removing tumourigenic pluripotent stem cells using rBC2LCN lectin.
  Regen Ther. 14: 306-314 (2020) doi: 10.1016/j.reth.2020.03.017
• Tateno H^*
  Expression and Purification of a Human Pluripotent Stem Cell-Specific Lectin Probe, rBC2LCN
  Methods Mol Biol. 9084: 30080-8 (2020) doi: 10.1007/978-1-0716-0430-4_44
• Mawaribuchi S, Haramoto Y, Tateno H, Onuma Y, Aiki Y, Ito Y
  rBC2LCN lectin as a potential probe of early-stage HER2-positive breast carcinoma
  FEBS Open Bio 10: 1056-1064 (2020) doi: 10.1002/2211-5463.12852
• Giron LB, Tanes CE, Schleimann MH, Engen PA, Mattei LM, Anzurez A, Damra M, Zhang HJ, Bittinger K, Bushman F, Kossenkov A, Denton PW, Tateno H, Keshavarzian A, Landay AL, Abdel-Mohsen M
  Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection
  Mucosal Immunology 129: 215-222 (2020) doi: 10.1038/s41385-020-0279-5
• Giron LB, Papasavvas E, Azzoni L, Yin XF, Anzurez A, Damra M, Mounzer K, Kostman JR, Sanne I, Firnhaber CS, Tateno H, Liu Q, Montaner LJ, Abdel-Mohsen M
  Plasma and antibody glycomic biomarkers of time to HIV rebound and viral setpoint
  Aids 34 34: 681-686 (2020) doi: 10.1097/QAD.0000000000002476
• Tateno H^*, Hiemori K, Minoshima F, Kiyoi K, Matoba K, Katayama J, Kumada Y
  Oriented immobilization of rBC2LCN lectin for highly sensitive detection of human pluripotent stem cells using cell culture supernatants
  J Biosci Bioeng. 129: 215-222 (2020) doi: 10.1016/j.jbiosc.2019.08.003
• Ogita Y^＃, Mawaribuchi S^＃, Nakasako K, Tamura K, Matsuda M, Katsumura T, Oota H, Watanabe G, Yoneda S, Takamatsu N, Ito M
  Parallel Evolution of Two dmrt1-Derived Genes, dmy and dm-W, for Vertebrate Sex Determination
  iScience 23: 100757 (2020) doi: 10.1016/j.isci.2019.100757
• Kanemaru K, Noguchi E, Tahara-Hanaoka S, Mizuno S, Tateno H, Denda-Nagai K, Irimura T, Matsuda H, Sugiyama F, Takahashi S, Shibuya K, Shibuya A
  Clec10a regulates mite-induced dermatitis
  Science Immunology 4 4: 0 (2019) doi: 10.1126/sciimmunol.aax6908
• Ogawa T, Sato R, Naganuma T, Liu K, Lakudzala AE, Muramoto K, Osada M, Yoshimi K, Hiemori K, Hirabayashi J, Tateno H
  Glycan Binding Profiling of Jacalin-Related Lectins from the Pteria Penguin Pearl Shell
  Int J Mol Sci. 20: 0 (2019) doi: 10.3390/ijms20184629
• Mawaribuchi S^*, Ito Y, Ito M＊
  Independent evolution for sex determination and differentiation in the DMRT family in animals
  Biology Open 8: 10 (2019) doi: 10.1242/bio.041962
• Mawaribuchi S, Aiki Y, Ikeda N, Ito Y
  mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
  Scientific Reports 189: 233-248 (2019) doi: 10.1038/s41598-019-48447-z
• Yamasaki K, Kubota T, Yamasaki T, Nagashima I, Shimizu H, Terada RI, Nishigami H, Kang J, Tateno M, Tateno H
  Structural basis for specific recognition of core fucosylation in N-glycans by Pholiota squarrosa lectin (PhoSL
  Glycobiology. 29: 576-587 (2019) doi: 10.1093/glycob/cwz025
• Oguma K, Sato T, Kawahara T, Haramoto Y, Yamanishi Y
  Identification of Aquatic Organisms Using a Magneto-Optical Element
  Sensors 19: 3254 (2019) doi: 10.3390/s19153254
• Khummuang S, Chuensirikulchai K, Pata S, Laopajon W, Chruewkamlow N, Mahasongkram K, Sugiura N, Watanabe H, Tateno H, Kamuthachad L, Wongratanacheewin S, Takheaw N, Kasinrerk W
  Characterization and functional analysis of novel circulating NK cell sub-populations
  Int Immunol. 31: 515-530 (2019) doi: 10.1093/intimm/dxz027
• Tateno H^*
  Human Stem Cell Glycome: From Structural Elucidation to Social Implementation
  Trends In Glycoscience And Glycotechnology 31: 0 (2019) doi: 10.4052/tigg.1939.2SE
• Mawaribuchi S, Onuma Y, Aiki Y, Kuriyama Y, Mutoh M, Fujii G, Ito, Y
  The rBC2LCN-positive subpopulation of PC-3 cells exhibits cancer stem-like properties
  Biochem Biophys Res Commun. 515: 176-182 (2019) doi: 10.1016/j.bbrc.2019.05.108
• Matsunami M, Suzuki M, Haramoto Y, Fukui A, Inoue T, Yamaguchi K, Uchiyama I, Mori K, Tashiro K, Ito Y, Takeuchi T, Suzuki KT, Agata K, Shigenobu S, Hayashi T
  A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology
  DNA Res. 26: 217-229 (2019) doi: 10.1093/dnares/dsz003
• Dion J, Minoshima F, Saito S, Kiyoi K, Hasehira K, Tateno H^*
  Photoactivable Elimination of Tumorigenic Human Induced Pluripotent Stem Cells by Using a Lectin-Doxorubicin Prodrug Conjugate
  Chembiochem. 20: 1606-1611 (2019) doi: 10.1002/cbic.201900086
• Yamazaki T, Inui M, Hiemori K, Tomono S, Itoh M, Ichimonji I, Nakashima A, Takagi H, Biswas M, Izawa K, Kitaura J, Imai T, Sugiura N, Tateno H, Akashi-Takamura S
  Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis
  J Biol Chem. 294: 6659-6669 (2019) doi: 10.1074/jbc.RA118.006375
• Kondo M, Matsuo M, Igarashi K, Haramoto Y, Yamamoto T, Yasuoka Y, Taira M
  De novo transcription of multiple Hox cluster genes takes place simultaneously in early Xenopus tropicalis embryos
  Biology Open 8: bio038422 (2019) doi: 10.1242/bio.038422
• Yokoyama H, Moreno-Andres D, Astrinidis SA, Hao Y, Weberruss M, Schellhaus AK, Lue H, Haramoto Y, Gruss OJ, Antonin W
  Chromosome alignment maintenance requires the MAP RECQL4, mutated in the Rothmund-Thomson syndrome
  Life Sci Alliance. 2: e201800120 (2019) doi: 10.26508/lsa.201800120
• Sakai K, Hiemori K, Tateno H, Hirabayashi J, Gonoi T
  Fucose-specific lectin of Aspergillus fumigatus: binding properties and effects on immune response stimulation
  Med Mycol. 57: 71-83 (2019) doi: 10.1093/mmy/myx163
• Omata Y, Aoki R, Aoki-Yoshida A, Hiemori K, Toyoda A, Tateno H, Suzuki C, Takayama Y
  Reduced fucosylation in the distal intestinal epithelium of mice subjected to chronic social defeat stress
  Sci Rep. 8: 13199 (2018) doi: 10.1038/s41598-018-31403-8
• Unno H, Nakamura A, Mori S, Goda S, Yamaguchi K, Hiemori K, Tateno H, Hatakeyama T
  Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Lectin AJLec from the Sea Anemone Anthopleura japonica
  Sci Rep. 8: 11516 (2018) doi: 10.1038/s41598-018-29498-0
• Hirabayashi J, Hu D, Tateno H, Kuwabara N, Kato R, Yagi F
  Carbohydrate Recognition Mechanism of the Mushroom Galectin ACG
  Trends In Glycoscience And Glycotechnology 30: 0 (2018) doi: 10.4052/tigg.1736.1SE
• Yamasaki K, Yamasaki T, Tateno H
  The trimeric solution structure and fucose-binding mechanism of the core fucosylation-specific lectin PhoSL
  Sci Rep. 8: 7740 (2018) doi: 10.1038/s41598-018-25630-2
• Nakagawa Y, Tateno H, Ebara M
  Investigation of Selective Recognition of Sugars Using Lectin-inspired Temperature-responsive Polymers
  Chemistry Letters 47: 134-137 (2018) doi: 10.1246/cl.170967
• Saito S, Hiemori K, Kiyoi K, Tateno H^*
  Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
  Sci Rep. 8: 3997 (2018) doi: 10.1038/s41598-018-22450-2
• Shimomura O, Oda T, Tateno H, Ozawa Y, Kimura S, Sakashita S, Noguchi M, Hirabayashi J, Asashima M, Ohkohchi N
  A Novel Therapeutic Strategy for Pancreatic Cancer: Targeting Cell Surface Glycan Using rBC2LC-N Lectin-Drug Conjugate (LDC
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  Folding and Binding Properties of Human Complement Receptor Type 1 Extracellular Domain
  Peripheral Membrane Proteins 1: 105-122, (2018) doi:10.5772/intechopen.75120
• Hasehira K, Hirabayashi J, Tateno H^*
  Structural and quantitative evidence of alpha2-6-sialylated N-glycans as markers of the differentiation potential of human mesenchymal stem cells
  Glycoconj J. 34: 797-806 (2017) doi: 10.1007/s10719-016-9699-6
• Nagae M, Mishra SK, Hanashima S, Tateno H, Yamaguchi Y
  Distinct roles for each N-glycan branch interacting with mannose-binding type Jacalin-related lectins Orysata and Calsepa
  Glycobiology. 27: 1120-1133 (2017) doi: 10.1093/glycob/cwx081
• Dion J, Advedissian T, Storozhylova N, Dahbi S, Lambert A, Deshayes F, Viguier M, Tellier C, Poirier F, Teletchea S, Dussouy C, Tateno H, Hirabayashi J, Grandjean C
  Development of a Sensitive Microarray Platform for the Ranking of Galectin Inhibitors: Identification of a Selective Galectin-3 Inhibitor
  Chembiochem. 18: 2428-2440 (2017) doi: 10.1002/cbic.201700544
• Fujimaki S, Kuwabara T
  Diabetes-Induced Dysfunction of Mitochondria and Stem Cells in Skeletal Muscle and the Nervous System
  Int J Mol Sci. 18: 0 (2017) doi: 10.3390/ijms18102147
• Fujimaki S, Kuwabara T
  Diabetes-Induced Dysfunction of Mitochondria and Stem Cells in Skeletal Muscle and the Nervous System
  INTERNATIONAL J. Molecular Sciences 18: 10 (2017) doi: 10.3390/ijms18102147
• Yagi H, Tateno H, Hayashi K, Hayashi T, Takahashi K, Hirabayashi J, Kato K, Tsuboi M
  Lectin microarray analysis of isolated polysaccharides from Sasa veitchii
  Biosci Biotechnol Biochem. 81: 1687-1689 (2017) doi: 10.1080/09168451.2017.1340089
• Hatakeyama T, Ichise A, Unno H, Goda S, Oda T, Tateno H, , Hirabayashi J, Sakai H, Nakagawa H
  Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus
  Protein Science 26: 1574-1583 (2017) doi: 10.1002/pro.3185
• Tateno H^*, Saito S
  Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells
  Molecules. 22: 0 (2017) doi: 10.3390/molecules22071151
• Haramoto Y, Saijyo T, Tanaka T, Furuno N, Suzuki A, Ito Y, Kondo M, Taira M, Takahashi S
  Identification and comparative analyses of Siamois cluster genes in Xenopus laevis and tropicalis
  Dev Biol. 426: 374-383 (2017) doi: 10.1016/j.ydbio.2016.07.015
• Itakura Y, Nakamura-Tsuruta S, Kominami J, Tateno H, Hirabayashi J
  Sugar-Binding Profiles of Chitin-Binding Lectins from the Hevein Family: A Comprehensive Study
  INTERNATIONAL J. Molecular Sciences 18: 10 (2017) doi: 10.3390/ijms18061160
• Sato T, Tateno H, Kaji H, Chiba Y, Kubota T, Hirabayashi J, Narimatsu H
  Engineering of recombinant Wisteria floribunda agglutinin specifically binding to GalNAcbeta1,4GlcNAc (LacdiNAc
  Glycobiology. 0: 0 (2017) doi: 10.1093/glycob/cwx038
• Nakata H, Lin CY, Abolhassani M, Ogawa T, Tateno H, Hirabayashi J, Muramoto K
  Isolation of Rice Bran Lectins and Characterization of Their Unique Behavior in Caco-2 Cells
  Int J Mol Sci. 18: 0 (2017) doi: 10.3390/ijms18051052
• Ishii N
  Electron microscopic and physicobiochemical studies of chaperonin GroEL and GroES complexes.
  Microscopy and imaging science: practical approaches to applied research and education 7: 112-119 (2017)
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• Ishii N
  GroEL and the GroEL-GroES Complex
  Macromolecular Protein Complexes: Structure And Function 83: 483 (2017) doi: 10.1007/978-3-319-46503-6_17
• Ishii N
  GroEL and the GroEL-GroES Complex
  Subcell Biochem. 7: 11563 (2017) doi: 10.1007/978-3-319-46503-6_17
• Yamamoto YY, Uno Y, Sha E, Ikegami K, Ishii N, Dohmae N, Sekiguchi H, Sasaki YC, Yohda M
  Asymmetry in the function and dynamics of the cytosolic group II chaperonin CCT/TRiC
  PloS One. 12: 0 (2017) doi: 10.1371/journal.pone.0176054