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Multicellular System Regulation Research Group


Project1:Development of glycan analytical technology
Researcher:Haruki Odaka, Yoshikazu Haramoto, Hiroaki Tateno

The sugar chain covers the outermost part of all cells and is called the "cell face" because it changes depending on the cell type and state (differentiation, carcinogenesis). It is expected as a drug discovery target for diseases such as. So far, we have constructed recombinant lectin libraries and have been working on the development of technology for profiling sugar chains by using these libraries. In the future, we will proceed with the development of new technology for analyzing sugar chains with single cell particle size.

Multicellular System Regulation Research Group02


Project2:Development of the quality control technology of stem cells
Researcher:Tomoko Watanabe, Yoshikazu Haramoto, Shuuji Mawaribuchi, Hiroaki Tateno

Human pluripotent cells and human mesenchymal stem cells are expected as cell sources for regenerative medicine. When transplanting these cells into humans, ensuring the safety and quality of the cells is extremely important. So far, we have succeeded in developing a technology for non-destructively detecting and removing the tumorigenic human pluripotent stem cells remaining in the cells for transplantation, and have successfully commercialized it in collaboration with a company. We will continue to develop new quality control technology.

Multicellular System Regulation Research Group03


Project3:Development of diagnostic technology for cancer, diabetes, neuronal diseases, and mental disorders.
Researcher:Mawaribuchi Shuuji、Odaka Haruki、Kuwabara Tomoko、Tateno Hiroaki

The development of diagnostic techniques for cancer, diabetes, and psychiatric/neurological disorders is important for definitive diagnosis, decision of treatment policy, improvement of QOL, and early rehabilitation. We aim to develop a new diagnostic technology by analyzing the cell function and gene/glycan expression of diseased cells (prostate cancer, breast cancer, pancreatic cancer, diabetes, mental disorders).

Multicellular System Regulation Research Group04


Project4:Separation and purification of exosomes, and development of utilization technology
Researcher:Noriyuki Ishii, Haruki Odaka, Tomoko Watanabe, Hiroaki Tateno

Exosomes are membrane-bound extracellular vesicles (EVs) produced in the endosomal compartment of most eukaryotic cells that are transported to other cells by the systemic circulation, and function as an intercellular communication tool in Embryological, Physiological, and Pathological processes. We collaborate with researchers inside and outside the laboratory to advance the technology for preparing EVs that are in an intrinsic state immediately after they are secreted from cells, develop technology for their utilization, and understand the functions of glycans of EVs. We will try to clarify the essence of the mysterious exosome.

Multicellular System Regulation Research Group05


Project5:Elucidation of mechanisms for organ development and tissue regeneration
Researcher:Yoshikazu Haramoto

To develop the methodology to generate organs and tissues applicable in regenerative medicine, we study in vivo differentiation mechanisms of organs and tissues. Amphibians are known for high regenerative capability among the Vertebrata. We analyze their regenerative mechanism and aim to apply the obtained insights to medicine. In addition, adult stem cells with self-renewal ability and pluripotency play an important role in maintaining homeostasis by compensating for cells lost in various tissues, are expected as useful stem cells for regenerative medicine. We aim to search for adult stem cells and to elucidate the microenvironment and signals that are important for self-renewal and maintenance of undifferentiated state of adult stem cells.

Multicellular System Regulation Research Group06


Name Position
TATENO Hiroaki Research Group Leader
SHIMIZU Hiroki Senior Researcher
ISHII Noriyuki Senior Researcher
KUWABARA Tomoko Senior Researcher
HARAMOTO Yoshikazu Senior Researcher
MAWARIBUCHI Shuuji Researcher
WATANABE Tomoko Researcher
ODAKA Haruki Researcher


  • Watanabe, T; Tateno, H.
    Elimination of cells deviated from human induced pluripotent stem cells with a photoactivatable IR700-labelled antibody.
    BIOCHEM BIOPHYS RES COMMUN. 2021 Mar 25;554:13-18. doi: 10.1016/j.bbrc.2021.03.078
  • Furuta T, Tateno H, Kiyoi K, Miyazaki Y, Kimura S, Kurimori K, Shimomura O, Oda T
    Glycans on CEA as a targeting ligand of pancreatic ductal adenocarcinoma in novel lectin-drug conjugated therapy.
    CANCER SCIENCE 112 718-718; FEB
  • Odaka H, Numakawa T, Soga M, Kido J, Matsumoto S, Kajihara R, Okumiya T, Tani N, Tanoue Y, Fukuda T, Furuya H, Inoue T, Era T
    An iPSC-based neural model of sialidosis uncovers glycolytic impairment-causing presynaptic dysfunction and deregulation of Ca(2+) dynamics
    Neurobiol Dis. 2021 Jan 28:105279. doi: 10.1016/j.nbd.2021.105279
  • Kimura S, Oda T, Shimomura O, Enomoto T, Hashimoto S, Kuroda Y, Yu Y, Kurimori K, Furuta T, Miyazaki Y, Tateno H
    Novel Pancreatic Cancer Therapy Targeting Cell Surface Glycans by Liposomes Modified with rBC2LCN Lectin
    Eur Surg Res. 2021 Jan 27:1-10. doi: 10.1159/000513430
  • Inagaki H, Haramoto Y, Kubota HY, Shigeri Y
    Complete mitochondrial genome sequence of Japanese forest green tree frog (Rhacophorus arboreus.
    Mitochondrial DNA B Resour 2020 Sep 16;5(3):3347-3348. doi: 10.1080/23802359.2020.1820396
  • Odaka H, Hiemori K, Shimoda A, Akiyoshi K, Tateno H
    Platelet-derived extracellular vesicles are increased in sera of Alzheimers disease patients, as revealed by Tim4-based assays.
    FEBS OPEN BIO 2020 Dec 20. doi: 10.1002/2211-5463.13068
  • Hasehira K, Furuta T, Shimomura O, Asada M, Oda T, Tateno H
    Quantitative structural analysis of glycans expressed within tumors derived from pancreatic cancer patient-derived xenograft mouse models
    Biochem Biophys Res Commun. 2020 Dec 4:S0006-291X(20)32131-8. doi: 10.1016/j.bbrc.2020.11.087
  • Kitaguchi D, Oda T, Enomoto T, Ohara Y, Owada Y, Akashi Y, Furuta T, Yu Y, Kimura S, Kuroda Y, Kurimori K, Miyazaki Y, Furuya K, Shimomura O, Tateno H
    Lectin Drug Conjugate Therapy for Colorectal Cancer
    Cancer Sci. 2020 Oct 15. (2020) doi: 10.1111/cas.14687
  • Inagaki H, Haramoto Y, Kubota HY, Shigeri Y
    Complete mitochondrial genome sequence of Japanese forest green tree frog (Rhacophorus arboreus)
    MITOCHONDRIAL DNA PART B-RESOURCES 5 (3) 3365-3366; (2020) doi:10.1080/23802359.2020.1820396
  • Watanabe T, Kakuta J, Saito S, Hasehira K, Kiyoi K, Imai T, Tateno H
    Monoclonal antibodies specific for podocalyxin expressed on human induced pluripotent stem cells.
    Biochem Biophys Res Commun. 2020 Sep 7:S0006-291X(20)31692-2. (2020) doi:10.1016/j.bbrc.2020.08.092
  • Giron LB, Colomb F, Papasavvas E, Azzoni L, Yin X, Fair M, Anzurez A, Damra M, Mounzer K, Kostman JR, Tebas P, ODoherty U, Tateno H, Liu Q, Betts MR, Montaner LJ, Abdel-Mohsen M
    Interferon-α alters host glycosylation machinery during treated HIV infection
    EBioMedicine. 59:102945. (2020) doi: 10.1016/j.ebiom.2020.102945
  • Wang J, Bhargava P, Yu Y, Sari AN, Zhang H, Ishii N, Yan K, Zhang Z, Ishida Y, Terao K, Kaul SC, Miyako E, Wadhwa R
    Novel Caffeic Acid Phenethyl Ester-Mortalin Antibody Nanoparticles Offer Enhanced Selective Cytotoxicity to Cancer Cells
    Cancers (Basel). 12(9):E2370. (2020) doi: 10.3390/cancers12092370
  • Watanabe T, Saito S, Hiemori K, Kiyoi K, Mawaribuchi S, Haramoto Y, Tateno H
    SSEA-1-positive fibronectin is secreted by cells deviated from the undifferentiated state of human induced pluripotent stem cells
    Biochem Biophys Res Commun. 529 (3) 575-581;(2020) doi:10.1016/j.bbrc.2020.06.074
  • Colomb F, Giron LB, Kuri-Cervantes L, Adeniji OS, Ma T, Dweep H, Battivelli E, Verdin E, Palmer CS, Tateno H, Kossenkov AV, Roan NR, Betts MR, Abdel-Mohsen M
    Sialyl-Lewis(X) Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy
    Cell Rep. Aug 4;32(5):107991. (2020) doi: 10.1016/j.celrep.2020.107991
  • Oinam L, Changarathil G, Raja E, Ngo YX, Tateno H, Sada A, Yanagisawa H
    Glycome profiling by lectin microarray reveals dynamic glycan alterations during epidermal stem cell aging
    Aging Cell. 35: 0 (2020) doi:10.1111/acel.13190
  • Haramoto Y, Onuma Y, Mawaribuchi S, Nakajima Y, Aiki Y, Higuchi K, Shimizu M, Tateno H, Hirabayashi J, Ito Y.
    A technique for removing tumourigenic pluripotent stem cells using rBC2LCN lectin.
    Regen Ther. 14: 306-314 (2020) doi: 10.1016/j.reth.2020.03.017
  • Tateno H*
    Expression and Purification of a Human Pluripotent Stem Cell-Specific Lectin Probe, rBC2LCN
    Methods Mol Biol. 9084: 30080-8 (2020) doi: 10.1007/978-1-0716-0430-4_44
  • Mawaribuchi S, Haramoto Y, Tateno H, Onuma Y, Aiki Y, Ito Y
    rBC2LCN lectin as a potential probe of early-stage HER2-positive breast carcinoma
    FEBS Open Bio 10: 1056-1064 (2020) doi: 10.1002/2211-5463.12852
  • Giron LB, Tanes CE, Schleimann MH, Engen PA, Mattei LM, Anzurez A, Damra M, Zhang HJ, Bittinger K, Bushman F, Kossenkov A, Denton PW, Tateno H, Keshavarzian A, Landay AL, Abdel-Mohsen M
    Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection
    Mucosal Immunology 129: 215-222 (2020) doi: 10.1038/s41385-020-0279-5
  • Giron LB, Papasavvas E, Azzoni L, Yin XF, Anzurez A, Damra M, Mounzer K, Kostman JR, Sanne I, Firnhaber CS, Tateno H, Liu Q, Montaner LJ, Abdel-Mohsen M
    Plasma and antibody glycomic biomarkers of time to HIV rebound and viral setpoint
    Aids 34 34: 681-686 (2020) doi: 10.1097/QAD.0000000000002476
  • Tateno H*, Hiemori K, Minoshima F, Kiyoi K, Matoba K, Katayama J, Kumada Y
    Oriented immobilization of rBC2LCN lectin for highly sensitive detection of human pluripotent stem cells using cell culture supernatants
    J Biosci Bioeng. 129: 215-222 (2020) doi: 10.1016/j.jbiosc.2019.08.003
  • Ogita Y, Mawaribuchi S, Nakasako K, Tamura K, Matsuda M, Katsumura T, Oota H, Watanabe G, Yoneda S, Takamatsu N, Ito M
    Parallel Evolution of Two dmrt1-Derived Genes, dmy and dm-W, for Vertebrate Sex Determination
    iScience 23: 100757 (2020) doi: 10.1016/j.isci.2019.100757
  • Kanemaru K, Noguchi E, Tahara-Hanaoka S, Mizuno S, Tateno H, Denda-Nagai K, Irimura T, Matsuda H, Sugiyama F, Takahashi S, Shibuya K, Shibuya A
    Clec10a regulates mite-induced dermatitis
    Science Immunology 4 4: 0 (2019) doi: 10.1126/sciimmunol.aax6908
  • Ogawa T, Sato R, Naganuma T, Liu K, Lakudzala AE, Muramoto K, Osada M, Yoshimi K, Hiemori K, Hirabayashi J, Tateno H
    Glycan Binding Profiling of Jacalin-Related Lectins from the Pteria Penguin Pearl Shell
    Int J Mol Sci. 20: 0 (2019) doi: 10.3390/ijms20184629
  • Mawaribuchi S*, Ito Y, Ito M*
    Independent evolution for sex determination and differentiation in the DMRT family in animals
    Biology Open 8: 10 (2019) doi: 10.1242/bio.041962
  • Mawaribuchi S, Aiki Y, Ikeda N, Ito Y
    mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
    Scientific Reports 189: 233-248 (2019) doi: 10.1038/s41598-019-48447-z
  • Yamasaki K, Kubota T, Yamasaki T, Nagashima I, Shimizu H, Terada RI, Nishigami H, Kang J, Tateno M, Tateno H
    Structural basis for specific recognition of core fucosylation in N-glycans by Pholiota squarrosa lectin (PhoSL
    Glycobiology. 29: 576-587 (2019) doi: 10.1093/glycob/cwz025
  • Oguma K, Sato T, Kawahara T, Haramoto Y, Yamanishi Y
    Identification of Aquatic Organisms Using a Magneto-Optical Element
    Sensors 19: 3254 (2019) doi: 10.3390/s19153254
  • Khummuang S, Chuensirikulchai K, Pata S, Laopajon W, Chruewkamlow N, Mahasongkram K, Sugiura N, Watanabe H, Tateno H, Kamuthachad L, Wongratanacheewin S, Takheaw N, Kasinrerk W
    Characterization and functional analysis of novel circulating NK cell sub-populations
    Int Immunol. 31: 515-530 (2019) doi: 10.1093/intimm/dxz027
  • Tateno H*
    Human Stem Cell Glycome: From Structural Elucidation to Social Implementation
    Trends In Glycoscience And Glycotechnology 31: 0 (2019) doi: 10.4052/tigg.1939.2SE
  • Mawaribuchi S, Onuma Y, Aiki Y, Kuriyama Y, Mutoh M, Fujii G, Ito, Y
    The rBC2LCN-positive subpopulation of PC-3 cells exhibits cancer stem-like properties
    Biochem Biophys Res Commun. 515: 176-182 (2019) doi: 10.1016/j.bbrc.2019.05.108
  • Matsunami M, Suzuki M, Haramoto Y, Fukui A, Inoue T, Yamaguchi K, Uchiyama I, Mori K, Tashiro K, Ito Y, Takeuchi T, Suzuki KT, Agata K, Shigenobu S, Hayashi T
    A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology
    DNA Res. 26: 217-229 (2019) doi: 10.1093/dnares/dsz003
  • Dion J, Minoshima F, Saito S, Kiyoi K, Hasehira K, Tateno H*
    Photoactivable Elimination of Tumorigenic Human Induced Pluripotent Stem Cells by Using a Lectin-Doxorubicin Prodrug Conjugate
    Chembiochem. 20: 1606-1611 (2019) doi: 10.1002/cbic.201900086
  • Yamazaki T, Inui M, Hiemori K, Tomono S, Itoh M, Ichimonji I, Nakashima A, Takagi H, Biswas M, Izawa K, Kitaura J, Imai T, Sugiura N, Tateno H, Akashi-Takamura S
    Receptor-destroying enzyme (RDE) from Vibrio cholerae modulates IgE activity and reduces the initiation of anaphylaxis
    J Biol Chem. 294: 6659-6669 (2019) doi: 10.1074/jbc.RA118.006375
  • Kondo M, Matsuo M, Igarashi K, Haramoto Y, Yamamoto T, Yasuoka Y, Taira M
    De novo transcription of multiple Hox cluster genes takes place simultaneously in early Xenopus tropicalis embryos
    Biology Open 8: bio038422 (2019) doi: 10.1242/bio.038422
  • Yokoyama H, Moreno-Andres D, Astrinidis SA, Hao Y, Weberruss M, Schellhaus AK, Lue H, Haramoto Y, Gruss OJ, Antonin W
    Chromosome alignment maintenance requires the MAP RECQL4, mutated in the Rothmund-Thomson syndrome
    Life Sci Alliance. 2: e201800120 (2019) doi: 10.26508/lsa.201800120
  • Sakai K, Hiemori K, Tateno H, Hirabayashi J, Gonoi T
    Fucose-specific lectin of Aspergillus fumigatus: binding properties and effects on immune response stimulation
    Med Mycol. 57: 71-83 (2019) doi: 10.1093/mmy/myx163
  • Omata Y, Aoki R, Aoki-Yoshida A, Hiemori K, Toyoda A, Tateno H, Suzuki C, Takayama Y
    Reduced fucosylation in the distal intestinal epithelium of mice subjected to chronic social defeat stress
    Sci Rep. 8: 13199 (2018) doi: 10.1038/s41598-018-31403-8
  • Unno H, Nakamura A, Mori S, Goda S, Yamaguchi K, Hiemori K, Tateno H, Hatakeyama T
    Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Lectin AJLec from the Sea Anemone Anthopleura japonica
    Sci Rep. 8: 11516 (2018) doi: 10.1038/s41598-018-29498-0
  • Hirabayashi J, Hu D, Tateno H, Kuwabara N, Kato R, Yagi F
    Carbohydrate Recognition Mechanism of the Mushroom Galectin ACG
    Trends In Glycoscience And Glycotechnology 30: 0 (2018) doi: 10.4052/tigg.1736.1SE
  • Yamasaki K, Yamasaki T, Tateno H
    The trimeric solution structure and fucose-binding mechanism of the core fucosylation-specific lectin PhoSL
    Sci Rep. 8: 7740 (2018) doi: 10.1038/s41598-018-25630-2
  • Nakagawa Y, Tateno H, Ebara M
    Investigation of Selective Recognition of Sugars Using Lectin-inspired Temperature-responsive Polymers
    Chemistry Letters 47: 134-137 (2018) doi: 10.1246/cl.170967
  • Saito S, Hiemori K, Kiyoi K, Tateno H*
    Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
    Sci Rep. 8: 3997 (2018) doi: 10.1038/s41598-018-22450-2
  • Shimomura O, Oda T, Tateno H, Ozawa Y, Kimura S, Sakashita S, Noguchi M, Hirabayashi J, Asashima M, Ohkohchi N
    A Novel Therapeutic Strategy for Pancreatic Cancer: Targeting Cell Surface Glycan Using rBC2LC-N Lectin-Drug Conjugate (LDC
    Mol Cancer Ther. 17: 183-195 (2018) doi: 10.1158/1535-7163.MCT-17-0232
  • Ishii N
    Folding and Binding Properties of Human Complement Receptor Type 1 Extracellular Domain
    Peripheral Membrane Proteins 1: 105-122, (2018) doi:10.5772/intechopen.75120
  • Hasehira K, Hirabayashi J, Tateno H*
    Structural and quantitative evidence of alpha2-6-sialylated N-glycans as markers of the differentiation potential of human mesenchymal stem cells
    Glycoconj J. 34: 797-806 (2017) doi: 10.1007/s10719-016-9699-6
  • Nagae M, Mishra SK, Hanashima S, Tateno H, Yamaguchi Y
    Distinct roles for each N-glycan branch interacting with mannose-binding type Jacalin-related lectins Orysata and Calsepa
    Glycobiology. 27: 1120-1133 (2017) doi: 10.1093/glycob/cwx081
  • Dion J, Advedissian T, Storozhylova N, Dahbi S, Lambert A, Deshayes F, Viguier M, Tellier C, Poirier F, Teletchea S, Dussouy C, Tateno H, Hirabayashi J, Grandjean C
    Development of a Sensitive Microarray Platform for the Ranking of Galectin Inhibitors: Identification of a Selective Galectin-3 Inhibitor
    Chembiochem. 18: 2428-2440 (2017) doi: 10.1002/cbic.201700544
  • Fujimaki S, Kuwabara T
    Diabetes-Induced Dysfunction of Mitochondria and Stem Cells in Skeletal Muscle and the Nervous System
    Int J Mol Sci. 18: 0 (2017) doi: 10.3390/ijms18102147
  • Fujimaki S, Kuwabara T
    Diabetes-Induced Dysfunction of Mitochondria and Stem Cells in Skeletal Muscle and the Nervous System
    INTERNATIONAL J. Molecular Sciences 18: 10 (2017) doi: 10.3390/ijms18102147
  • Yagi H, Tateno H, Hayashi K, Hayashi T, Takahashi K, Hirabayashi J, Kato K, Tsuboi M
    Lectin microarray analysis of isolated polysaccharides from Sasa veitchii
    Biosci Biotechnol Biochem. 81: 1687-1689 (2017) doi: 10.1080/09168451.2017.1340089
  • Hatakeyama T, Ichise A, Unno H, Goda S, Oda T, Tateno H, , Hirabayashi J, Sakai H, Nakagawa H
    Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus
    Protein Science 26: 1574-1583 (2017) doi: 10.1002/pro.3185
  • Tateno H*, Saito S
    Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells
    Molecules. 22: 0 (2017) doi: 10.3390/molecules22071151
  • Haramoto Y, Saijyo T, Tanaka T, Furuno N, Suzuki A, Ito Y, Kondo M, Taira M, Takahashi S
    Identification and comparative analyses of Siamois cluster genes in Xenopus laevis and tropicalis
    Dev Biol. 426: 374-383 (2017) doi: 10.1016/j.ydbio.2016.07.015
  • Itakura Y, Nakamura-Tsuruta S, Kominami J, Tateno H, Hirabayashi J
    Sugar-Binding Profiles of Chitin-Binding Lectins from the Hevein Family: A Comprehensive Study
    INTERNATIONAL J. Molecular Sciences 18: 10 (2017) doi: 10.3390/ijms18061160
  • Sato T, Tateno H, Kaji H, Chiba Y, Kubota T, Hirabayashi J, Narimatsu H
    Engineering of recombinant Wisteria floribunda agglutinin specifically binding to GalNAcbeta1,4GlcNAc (LacdiNAc
    Glycobiology. 0: 0 (2017) doi: 10.1093/glycob/cwx038
  • Nakata H, Lin CY, Abolhassani M, Ogawa T, Tateno H, Hirabayashi J, Muramoto K
    Isolation of Rice Bran Lectins and Characterization of Their Unique Behavior in Caco-2 Cells
    Int J Mol Sci. 18: 0 (2017) doi: 10.3390/ijms18051052
  • Ishii N
    Electron microscopic and physicobiochemical studies of chaperonin GroEL and GroES complexes.
    Microscopy and imaging science: practical approaches to applied research and education 7: 112-119 (2017)
  • Ishii N
    GroEL and the GroEL-GroES Complex
    Macromolecular Protein Complexes: Structure And Function 83: 483 (2017) doi: 10.1007/978-3-319-46503-6_17
  • Ishii N
    GroEL and the GroEL-GroES Complex
    Subcell Biochem. 7: 11563 (2017) doi: 10.1007/978-3-319-46503-6_17
  • Yamamoto YY, Uno Y, Sha E, Ikegami K, Ishii N, Dohmae N, Sekiguchi H, Sasaki YC, Yohda M
    Asymmetry in the function and dynamics of the cytosolic group II chaperonin CCT/TRiC
    PloS One. 12: 0 (2017) doi: 10.1371/journal.pone.0176054
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