MENU
- Group Introduction
- Molecular and Cellular Glycoproteomics Research Group
- Applied Molecular Function Research Group
- Stem Cell Biotechnology Research Group
- Multicellular System Regulation Research Group
- Healthy Food Science Research Group
- Leading-edge Biotechnology Research Group
- Biological Data Science Research Group
- AIST-INDIA Diverse Assets & Applications International Laboratory
Stem Cell Biotechnology Research Group
Project
Project1:Reconstruction of in vitro autonomic nervous system
Researcher:Yasuyuki S. Kida, Yuzo Takayama, Yuka Akagi, Yuma Nihashi
From the viewpoint of animal welfare, there is increasing attention to developing alternative methods for animal experiments that reproduce the physiological functions of the body in vitro. We have developed a method for inducing sympathetic and parasympathetic nerves from human stem cells. In this research project, we will develop Micro-physiological systems that mimic the vagal nerve tract of a living body such as the gut-brain axis and its evaluation method.

Project
Project2:Development of innovative cancer treatment methods using reconstructed tumor microenvironment models
Researcher:Yasuyuki S. Kida, Yutaro Kumagai, Nobuhito Mori
In pancreatic cancer, fibrotic stroma becomes a barrier, inhibiting penetration of the therapeutic drug into the tumor. We have been constructing experimental systems for studying the tumor microenvironment. In this research project, we will develop cell induction methods and three-dimensional tissue construction methods for cancer research as well as the development of cancer treatments.

Member
Name | Position |
---|---|
KIDA Yasuyuki | Research Group Leader |
TAKAYAMA Yuzo | Senior Researcher |
KUMAGAI Yutaro | Senior Researcher |
MORI Nobuhito | Senior Researcher |
AKAGI Yuuka | Researcher |
MORIKAWA Kumi | Researcher |
NIHASHI Yuuma | Researcher |
Results
- Ishioka, M; Nihashi, Y; Sunagawa, Y; Umezawa, K; Shimosato, T; Kagami, H; Morimoto, T; Takaya, T.
Myogenetic Oligodeoxynucleotide Induces Myocardial Differentiation of Murine Pluripotent Stem Cells.
INT J MOL SCI. 2023 Sep 21;24(18):14380. doi: 10.3390/ijms241814380 - Nihashi, Y, Song, X; Yamamoto, M; Setoyama, D; Kida, YS.
Decoding Metabolic Symbiosis between Pancreatic Cancer Cells and Cancer-Associated Fibroblasts Using Cultured Tumor Microenvironment.
INT J MOL SCI. 2023 Jul 3;24(13):11015. doi: 10.3390/ijms241311015 - Takayama, Y, Akagi, Y, Kida, YS.
Deciphering the Molecular Mechanisms of Autonomic Nervous System Neuron Induction through Integrative Bioinformatics Analysis.
INT J MOL SCI. 2023 May 21;24(10):9053. doi: 10.3390/ijms24109053 - Kumagai, Y; Saito, Y; Kida, YS.
A multiomics atlas of brown adipose tissue development over time.
ENDOCRINOLOGY. 2023 Apr 21:bqad064. doi: 10.1210/endocr/bqad064 - Sogo, T; Nakao, S; Tsukamoto, T; Ueyama, T; Harada, Y; Ihara, D; Ishida, T; Nakahara, M; Hasegawa, K; Akagi, Y; Kida, YS; Nakagawa, O; Nagamune, T; Kawahara, M; Kawamura, T.
Canonical Wnt signaling activation by chimeric antigen receptors for efficient cardiac differentiation from mouse embryonic stem cells.
INFLAMM REGEN. 2023 Feb 10;43(1):11. doi: 10.1186/s41232-023-00258-6 - Nohira, N; Shinji, S; Nakamura, S; Nihashi, Y; Shimosato, T; Takaya, T.
Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells.
BIOMEDICINES. 2022 Oct 25;10(11):2691. doi: 10.3390/biomedicines10112691 - Imai, Y;Mori, N; Nihashi, Y; Kumagai, Y; Shibuya, Y; Oshima, J; Sasaki, M; Sasaki, K; Aihara, Y; Sekido, M; Kida, YS.
Therapeutic Potential of Adipose Stem Cell-Derived Conditioned Medium on Scar Contraction Model.
BIOMEDICINES. 2022 Sep 24;10(10):2388. doi: 10.3390/biomedicines10102388 - Sugisawa, E; Kondo, T; Kumagai, Y; Kato, H; Takayama, Y; Isohashi, K; Shimosegawa, E; Takemura, N; Hayashi, Y; Sasaki, T; Martino, MM; Tominaga, M; Maruyama, K.
Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
CELL REP. 2022 Mar 8;38(10):110462. doi: 10.1016/j.celrep.2022.110462 - Miyazaki, Y; Mori, N; Akagi, Y; Oda, T; Kida, YS.
Potential Metabolite Markers for Pancreatic Cancer Identified by Metabolomic Analysis of Induced Cancer-Associated Fibroblasts.
CANCERS. 2022 Mar 8;14(6):1375. doi: 10.3390/cancers14061375 - Akagi, Y; Mori, N; Kawamura, T; Takayama, Y; Kida, YS.
Non-invasive cell classification using the Paint Raman Express Spectroscopy System (PRESS)
SCI REP. 2021 Apr 23;11(1):8818. doi: 10.1038/s41598-021-88056-3 - Mori, N; Kida, YS.
Applicability of Artificial Vascularized Liver Tissue to Proteomic Analysis.
MICROMACHINES (Basel). 2021 Apr 11;12(4):418. doi: 10.3390/mi12040418 - Miyazaki Y, Oda T, Inagaki Y, Kushige H, Saito Y, Mori N, Takayama Y, Kumagai Y, Mitsuyama T, Kida YS
Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.
SCI REP. 2021 Feb 25;11(1):4690. doi: 10.1038/s41598-021-84058-3 - Kumagai Y, Hirasawa T, Wachi M
Requirement of the LtsA Protein for Formation of the Mycolic Acid-Containing Layer on the Cell Surface of Corynebacterium glutamicum.
MICROORGANISMS. 2021 Feb 16;9(2):409. doi: 10.3390/microorganisms9020409 - Baba T, Takagi T, Sumaru K, Kanamori T, Dewa T, Nango M
Membrane properties of ether-type phosphatidylcholine bearing partially fluorinated C(18)-monoacetylenic chains and their applicability to membrane protein reconstitution matrices
Colloids Surf B Biointerfaces. 2020 Nov 13:111459. doi: 10.1016/j.colsurfb.2020.111459 - Takayama Y, Akagi Y, Shibuya Y, Kida YS
Exposure to small molecule cocktails allows induction of neural crest lineage cells from human adipose-derived mesenchymal stem cells
PLoS One. ;15(10):e0241125. (2020)doi: 10.1371/journal.pone.0241125 - Miyazaki Y, Oda T, Mori N, Kida YS
Adipose-derived mesenchymal stem cells differentiate into pancreatic cancer-associated fibroblasts in vitro
FEBS Open Bio. 2020 Sep 15. (2020) doi: 10.1002/2211-5463.12976 - Mori N, Kida YS
Expression of genes involved in drug metabolism differs between perfusable 3D liver tissue and conventional 2D-cultured hepatocellular carcinoma cells
FEBS Open Bio. 0 (2020) Aug 14. doi: 10.1002/2211-5463.12948 - Maruyama K, Kidoya H, Takemura N, Sugisawa E, Takeuchi O, Kondo T, Eid MMA, Tanaka H, Martino MM, Takakura N, Takayama Y, Akira S, Vandenbon A, Kumagai Y
Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
Cell Rep. 32: 107906 (2020) doi:10.1016/j.celrep.2020.107906 - Sugisawa E, Takayama Y, Takemura N, Kondo T, Hatakeyama S, Kumagai Y, Sunagawa M, Tominaga M, Maruyama K
RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis
Cell. 8674: 30754-6 (2020) doi:10.1016/j.cell.2020.06.022 - Takayama Y, Kushige H, Akagi Y, Suzuki Y, Kumagai Y, Kida YS*
Selective induction of Human Autonomic neurons enables precise control of cardiomyocyte Beating
Sci Rep.10: 9464 (2020) doi:10.1038/s41598-020-66303-3 - Mori N, Akagi Y, Imai Y, Takayama Y, Kida YS*
Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue
Sci Rep. 10: 5646 (2020) doi: 10.1038/s41598-020-62286-3 - Baba T, Takagi T, Sumaru K, Kanamori T
Effect of the fluorination degree of partially fluorinated octyl-phosphocholine surfactants on their interfacial properties and interactions with purple membrane as a membrane protein model
Chemistry And Physics Lipids 227 0: 0 (2020) doi: 10.1016/j.chemphyslip.2020.104870 - Marx U, Akabane T, Andersson TB, Baker E, Beilmann M, Beken S, Brendler-Schwaab S, Cirit M, David R, Dehne EM, Durieux I, Ewart L, Fitzpatrick SC, Frey O, Fuchs F, Griffith LG, Hamilton GA, Hartung T, Hoeng J, Hogberg H, Hughes DJ, Ingber DE, Iskandar A, Kanamori T, Kojima H, Kuehnl J, Leist M, Li B, Loskill P, Mendrick DL, Neumann T, Pallocca G, Rusyn I, Smirnova L, Steger-Hartmann T, Tagle DA, Tonevitsky A, Tsyb S, Trapecar M, Van de Water B, Van den Eijnden-van Raaij J, Vulto P, Watanabe K, Wolf A, Zhou X, Roth A
Biology-inspired microphysiological systems to advance patient benefit and animal welfare in drug development
Altex. 12: 0 (2020) doi: 10.14573/altex.2001241 - Komatsu H, Sakai K, Baba T, Takagi T, Kanamori T
Aggregation behavior of short-chained archaeal phospholipid analogs: Contribution methyl branches to lipid hydrophobicity and membrane formability
Colloid And Interface Science Communications 129: 3827-3832 (2019) doi: 10.1016/j.colcom.2019.100200 - Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center
J. Physical Chemistry A 123: 3985-3991 (2019) doi: 10.1021/acs.jpca.8b11613 - Ishida S, Kanamori T
Microphysiological system as a promising technology for drug assay
Nihon Yakurigaku Zasshi. 154: 345-351 (2019) doi: 10.1254/fpj.154.345 - Kise R, Fukumi A, Shioya N, Shimoaka T, Sonoyama M, Amii H, , Takagi T, Kanamori T, Eda K, Hasegawa T
Fluorous Property of a Short Perfluoroalkyl-Containing Compound Realized by Self-Assembled Monolayer Technique on a Silicon Substrate
Bulletin The Chemical Society Japan 92: 785-789 (2019) doi: 10.1246/bcsj.20180356 - Tsukamoto T, Sogo T, Ueyama T, Nakao S, Harada Y, Ihara D, Akagi, Y, Kida YS, Hasegawa K, Nagamune T, Kawahara M*, Kawamura T*
Chimeric G-CSF Receptor-Mediated STAT3 Activation Contributes to Efficient Induction of Cardiomyocytes from Mouse Induced Pluripotent Stem Cells
Biotechnology J. 93: 10 (2019) doi: 10.1002/biot.201900052 - Takahashi H, Yoshino M, Morita K, Takagi T, Yokoyama Y, Kikukawa T, Amii H, Kanamori T, Sonoyama M
Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
Biochim Biophys Acta Biomembr. 1861: 631-642 (2019) doi: 10.1016/j.bbamem.2018.12.015 - Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
Commun Biol. 1: 218 (2018) doi: 10.1038/s42003-018-0222-4 - Mori N, Morimoto Y, Takeuchi S*
Perfusable and stretchable 3D culture system for skin-equivalent
Biofabrication. 11: 011001 (2018) doi: 10.1088/1758-5090/aaed12 - Vandenbon A#, Kumagai Y#, Lin M, Suzuki Y, Nakai K*
Waves of chromatin modifications in mouse dendritic cells in response to LPS stimulation
Genome Biol. 19: 138 (2018) doi: 10.1186/s13059-018-1524-z - Maruyama K*, Takayama Y, Sugisawa E, Yamanoi Y, Yokawa T, Kondo T, Ishibashi KI, Sahoo BR, Takemura N, Mori Y, Kanemaru H, Kumagai Y, Martino MM, Yoshioka Y, Nishijo H, Tanaka H, Sasaki A, Ohno N, Iwakura Y, Moriyama Y, Nomura M, Akira S, Tominaga M*
The ATP Transporter VNUT Mediates Induction of Dectin-1-Triggered Candida Nociception
iScience. 0: 0 (2018) doi: 10.1016/j.isci.2018.08.007 - Wagatsuma A*, Takayama Y, Hoshino T, Shiozuka M, Yamada S, Matsuda R, Mabuchi K
Pharmacological targeting of HSP90 with 17-AAG induces apoptosis of myogenic cells through activation of the intrinsic pathway
Mol Cell Biochem. 163: 170-182 (2018) doi: 10.1007/s11010-017-3250-3 - Sumaru K, Takagi T, Morishita K, Satoh T, Kanamori T
Fabrication of pocket-like hydrogel microstructures through photolithography
St Matter 14: 5710-5714 (2018) doi: 10.1039/c8sm00865e - Sumaru K, Takagi T, Morishita K, Kanamori T
Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
Biomacromolecules. 19: 2913-2922 (2018) doi: 10.1021/acs.biomac.8b00470 - Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
Commun Biol. 0: 0 (2018) doi: 10.1038/s42003-018-0222-4 - Sumaru K, Takagi T, Satoh T, Kanamori T
Photo- and Thermoresponsive Dehydration of Spiropyran-Functionalized Polymer Regulated by Molecular Recognition
Macromol Rapid Commun. 39: 0 (2018) doi: 10.1002/marc.201700234 - Ito N, Katoh K, Kushige H, Saito Y, Umemoto T, Matsuzaki Y, Kiyonari H, Kobayashi D, Soga M, Era T, Araki N, Furuta Y, Suda T, Kida Y, Ohta K*
Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency
Sci Rep. 8: 1634 (2018) doi: 10.1038/s41598-018-20057-1 - Ueyama T, Nakao S, Tsukamoto T, Ihara D, Harada Y, Akagi Y, Nakagawa S, Kida YS, Sogo T and Kawamura T*
PTEN/Akt Axis is Involved in Somatic Cell Reprogramming to Mouse iPS Cells
Biomed J Sci & Tech. Res. 11: 5 (2018) DOI: 10.26717/BJSTR.2018.11.002162 - Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
Study of Perfluoroalkyl Chain-Specific Band Shift in Infrared Spectra on the Chain Length
J. Physical Chemistry A 121: 8425-8431 (2017) doi: 10.1021/acs.jpca.7b08940 - Sumaru K, Morishita K, Takagi T, Satoh T, Kanamori T
Sectioning of cultured cell monolayer using photo-acid-generating substrate and micro-patterned light projection
European Polymer J. 121: 16964-16969 (2017) doi: 10.1016/j.eurpolymj.2017.02.021 - Watanabe Y, Miyasaka KY, Kubo A, Kida YS, Nakagawa O, Hirate Y, Sasaki H, Ogura T*
Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm
Sci Rep. 0: 0 (2017) doi: 10.1038/srep46135 - Kondo Y, Yada Y, Haga T, Takayama Y, Isomura T, Jimbo Y, Fukayama O, Hoshino T*, Mabuchi K
Temporal relation between neural activity and neurite pruning on a numerical model and a microchannel device with micro electrode array
Biochem Biophys Res Commun. 486: 539-544 (2017) doi: 10.1016/j.bbrc.2017.03.082 - Takayama Y#, Wakabayashi T#, Kushige H, Saito Y, Shibuya Y, Shibata S, Akamatsu W, Okano H, Kida YS*
Brief Exposure to Small Molecules Allows Induction of Mouse Embryonic Fibroblasts Into Neural Crest-Like Precursors
FEBS Lett. 591: 590-602 (2017) doi: 10.1002/1873-3468.12572