• Access
• Japanese

Project

Our goal is establishing global informatic data and usages of adipose tissue. We are also challenging to develop stem cell technologies for regenerative medicine.

Project

By connecting the peripheral nervous system (PNS) to organ, we have attempted to apply it to drug discovery assay systems. In addition, we are focusing on the development of induction methods for neurons in the autonomic nervous system.

Project

We investigate a technique for fabricating large vascular channels and capillaries in artificial tissues by a tissue culture device. Our system enables supply of oxygen and nutrients to maintain large tissues and also drugs for toxicity and efficacy test.

Project

We develop non-invasive and high accurate cell fractionation technology by applying confocal Raman spectroscopy to living-tissue derived cell. This technology can lead the development of new drugs using processed cell.

Project

we attempt to uncover pivotal mechanisms in the tumor environment and discover medical seeds that break through the important clinical characteristics.

Project

To reveal mechanism of immune system, which is involved in not only detecting foreign bodies such as pathogens, but also in cancer and metabolic diseases, we integrate experimental, information theoretical, and mathematical methods.

Project

We aim to develop pressure-driven microphysiological systems (PD-MPS) that can create and maintain high-level cell and organ functions as an alternative to animal experiments and a novel research tool in drug discovery.

Member

Results

• Akagi, Y; Mori, N; Kawamura, T; Takayama, Y; Kida, YS.
  Non-invasive cell classification using the Paint Raman Express Spectroscopy System (PRESS)
  SCI REP. 2021 Apr 23;11(1):8818. doi: 10.1038/s41598-021-88056-3
• Mori, N; Kida, YS.
  Applicability of Artificial Vascularized Liver Tissue to Proteomic Analysis.
  MICROMACHINES (Basel). 2021 Apr 11;12(4):418. doi: 10.3390/mi12040418
• Miyazaki Y, Oda T, Inagaki Y, Kushige H, Saito Y, Mori N, Takayama Y, Kumagai Y, Mitsuyama T, Kida YS
  Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.
  SCI REP. 2021 Feb 25;11(1):4690. doi: 10.1038/s41598-021-84058-3
• Kumagai Y, Hirasawa T, Wachi M
  Requirement of the LtsA Protein for Formation of the Mycolic Acid-Containing Layer on the Cell Surface of Corynebacterium glutamicum.
  MICROORGANISMS. 2021 Feb 16;9(2):409. doi: 10.3390/microorganisms9020409
• Shinohara M, Arakawa H, Oda Y, Shiraki N, Sugiura S, Nishiuchi T, Satoh T, Iino K, Leo S, Kato Y, Araya K, Kawanishi T, Nakatsuji T, Mitsuta M, Inamura K, Goto T, Shinha K, Nihei W, Komori K, Nishikawa M, Kume S, Kato Y, Kanamori T, Sakai Y, Kimura H
  Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system
  Sci Rep. 2021 Mar 8;11(1):5437. doi: 10.1038/s41598-021-84861-y
• Baba T, Takagi T, Sumaru K, Kanamori T, Dewa T, Nango M
  Membrane properties of ether-type phosphatidylcholine bearing partially fluorinated C(18)-monoacetylenic chains and their applicability to membrane protein reconstitution matrices
  Colloids Surf B Biointerfaces. 2020 Nov 13:111459. doi: 10.1016/j.colsurfb.2020.111459
• Figarol A, Piantino M, Furihata T, Satoh T, Sugiura S, Kanamori T, Matsusaki M
  Interstitial flow regulates in vitro three-dimensional self-organized brain micro-vessels
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 533 (3) 600-606; (2020) doi:10.1016/j.bbrc.2020.09.061 DEC 10
• Takayama Y, Akagi Y, Shibuya Y, Kida YS
  Exposure to small molecule cocktails allows induction of neural crest lineage cells from human adipose-derived mesenchymal stem cells
  PLoS One. ;15(10):e0241125. (2020)doi: 10.1371/journal.pone.0241125
• Miyazaki Y, Oda T, Mori N, Kida YS
  Adipose-derived mesenchymal stem cells differentiate into pancreatic cancer-associated fibroblasts in vitro
  FEBS Open Bio. 2020 Sep 15. (2020) doi: 10.1002/2211-5463.12976
• Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
  Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
  ROYAL SOCIETY OPEN SCIENCE 7 (7); (2020) doi: 10.1098/rsos.200027
• Mori N, Kida YS
  Expression of genes involved in drug metabolism differs between perfusable 3D liver tissue and conventional 2D-cultured hepatocellular carcinoma cells
  FEBS Open Bio. 0 (2020) Aug 14. doi: 10.1002/2211-5463.12948
• Maruyama K, Kidoya H, Takemura N, Sugisawa E, Takeuchi O, Kondo T, Eid MMA, Tanaka H, Martino MM, Takakura N, Takayama Y, Akira S, Vandenbon A, Kumagai Y
  Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
  Cell Rep. 32: 107906 (2020) doi:10.1016/j.celrep.2020.107906
• Sugisawa E, Takayama Y, Takemura N, Kondo T, Hatakeyama S, Kumagai Y, Sunagawa M, Tominaga M, Maruyama K
  RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis
  Cell. 8674: 30754-6 (2020) doi:10.1016/j.cell.2020.06.022
• Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
  Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
  Royal Society Open Science 7 7: 0 (2020) doi:10.1098/rsos.200027
• Watanabe U,Sugiura S, Kakehata M, Yanagawa F, Takagi T, Sumaru K, Satoh T, Tamura M, Hosokawa Y, Torizuka K, Kanamori T
  Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering
  Micromachines 11: 0 (2020) doi:10.3390/mi11070679
• Takayama Y, Kushige H, Akagi Y, Suzuki Y, Kumagai Y, Kida YS^*
  Selective induction of Human Autonomic neurons enables precise control of cardiomyocyte Beating
  Sci Rep.10: 9464 (2020) doi:10.1038/s41598-020-66303-3
• Mori N, Akagi Y, Imai Y, Takayama Y, Kida YS^*
  Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue
  Sci Rep. 10: 5646 (2020) doi: 10.1038/s41598-020-62286-3
• Baba T, Takagi T, Sumaru K, Kanamori T
  Effect of the fluorination degree of partially fluorinated octyl-phosphocholine surfactants on their interfacial properties and interactions with purple membrane as a membrane protein model
  Chemistry And Physics Lipids 227 0: 0 (2020) doi: 10.1016/j.chemphyslip.2020.104870
• Marx U, Akabane T, Andersson TB, Baker E, Beilmann M, Beken S, Brendler-Schwaab S, Cirit M, David R, Dehne EM, Durieux I, Ewart L, Fitzpatrick SC, Frey O, Fuchs F, Griffith LG, Hamilton GA, Hartung T, Hoeng J, Hogberg H, Hughes DJ, Ingber DE, Iskandar A, Kanamori T, Kojima H, Kuehnl J, Leist M, Li B, Loskill P, Mendrick DL, Neumann T, Pallocca G, Rusyn I, Smirnova L, Steger-Hartmann T, Tagle DA, Tonevitsky A, Tsyb S, Trapecar M, Van de Water B, Van den Eijnden-van Raaij J, Vulto P, Watanabe K, Wolf A, Zhou X, Roth A
  Biology-inspired microphysiological systems to advance patient benefit and animal welfare in drug development
  Altex. 12: 0 (2020) doi: 10.14573/altex.2001241
• Arakawa H, Sugiura S, Kawanishi T, Shin K, Toyoda H, Satoh T, Sakai Y, Kanamori T, Kato Y
  Kinetic analysis of sequential metabolism of triazolam and its extrapolation to humans using an entero-hepatic two-organ microphysiological system
  Lab Chip. 3: 25 (2020) doi: 10.1039/c9lc00884e
• Komatsu H, Sakai K, Baba T, Takagi T, Kanamori T
  Aggregation behavior of short-chained archaeal phospholipid analogs: Contribution methyl branches to lipid hydrophobicity and membrane formability
  Colloid And Interface Science Communications 129: 3827-3832 (2019) doi: 10.1016/j.colcom.2019.100200
• Hirama H, Satoh T, Sugiura S, Shin K, Onuki-Nagasaki R, Kanamori T, Inoue T
  Glass-based organ-on-a-chip device for restricting small molecular absorption
  J Biosci Bioeng. 127: 641-646 (2019) doi: 10.1016/j.jbiosc.2018.10.019
• Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
  Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center
  J. Physical Chemistry A 123: 3985-3991 (2019) doi: 10.1021/acs.jpca.8b11613
• Ishida S, Kanamori T
  Microphysiological system as a promising technology for drug assay
  Nihon Yakurigaku Zasshi. 154: 345-351 (2019) doi: 10.1254/fpj.154.345
• Kise R, Fukumi A, Shioya N, Shimoaka T, Sonoyama M, Amii H, , Takagi T, Kanamori T, Eda K, Hasegawa T
  Fluorous Property of a Short Perfluoroalkyl-Containing Compound Realized by Self-Assembled Monolayer Technique on a Silicon Substrate
  Bulletin The Chemical Society Japan 92: 785-789 (2019) doi: 10.1246/bcsj.20180356
• Tsukamoto T, Sogo T, Ueyama T, Nakao S, Harada Y, Ihara D, Akagi, Y, Kida YS, Hasegawa K, Nagamune T, Kawahara M*, Kawamura T*
  Chimeric G-CSF Receptor-Mediated STAT3 Activation Contributes to Efficient Induction of Cardiomyocytes from Mouse Induced Pluripotent Stem Cells
  Biotechnology J. 93: 10 (2019) doi: 10.1002/biot.201900052
• Takahashi H, Yoshino M, Morita K, Takagi T, Yokoyama Y, Kikukawa T, Amii H, Kanamori T, Sonoyama M
  Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
  Biochim Biophys Acta Biomembr. 1861: 631-642 (2019) doi: 10.1016/j.bbamem.2018.12.015
• Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
  Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
  Commun Biol. 1: 218 (2018) doi: 10.1038/s42003-018-0222-4
• Shibuta M, Tamura M, Kanie K, Yanagisawa M, Matsui H, Satoh T, , Takagi T, Kanamori T, Sugiura S, Kato R
  Imaging cell picker: A morphology-based automated cell separation system on a photodegradable hydrogel culture platform
  J. Bioscience And Bioengineering 126: 653-660 (2018) doi: 10.1016/j.jbiosc.2018.05.004
• Mori N, Morimoto Y, Takeuchi S*
  Perfusable and stretchable 3D culture system for skin-equivalent
  Biofabrication. 11: 011001 (2018) doi: 10.1088/1758-5090/aaed12
• Vandenbon A^#, Kumagai Y^#, Lin M, Suzuki Y, Nakai K^*
  Waves of chromatin modifications in mouse dendritic cells in response to LPS stimulation
  Genome Biol. 19: 138 (2018) doi: 10.1186/s13059-018-1524-z
• Tashiro S, Le MNT, Kusama Y, Nakatani E, Suga M, Furue MK, Satoh T, Sugiura S, Kanamori T, Ohnuma K
  High cell density suppresses BMP4-induced differentiation of human pluripotent stem cells to produce macroscopic spatial patterning in a unidirectional perfusion culture chamber
  J Biosci Bioeng. 126: 379-388 (2018) doi: 10.1016/j.jbiosc.2018.03.007
• Maruyama K^*, Takayama Y, Sugisawa E, Yamanoi Y, Yokawa T, Kondo T, Ishibashi KI, Sahoo BR, Takemura N, Mori Y, Kanemaru H, Kumagai Y, Martino MM, Yoshioka Y, Nishijo H, Tanaka H, Sasaki A, Ohno N, Iwakura Y, Moriyama Y, Nomura M, Akira S, Tominaga M*
  The ATP Transporter VNUT Mediates Induction of Dectin-1-Triggered Candida Nociception
  iScience. 0: 0 (2018) doi: 10.1016/j.isci.2018.08.007
• Wagatsuma A^*, Takayama Y, Hoshino T, Shiozuka M, Yamada S, Matsuda R, Mabuchi K
  Pharmacological targeting of HSP90 with 17-AAG induces apoptosis of myogenic cells through activation of the intrinsic pathway
  Mol Cell Biochem. 163: 170-182 (2018) doi: 10.1007/s11010-017-3250-3
• Sumaru K, Takagi T, Morishita K, Satoh T, Kanamori T
  Fabrication of pocket-like hydrogel microstructures through photolithography
  St Matter 14: 5710-5714 (2018) doi: 10.1039/c8sm00865e
• Sumaru K, Takagi T, Morishita K, Kanamori T
  Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
  Biomacromolecules. 19: 2913-2922 (2018) doi: 10.1021/acs.biomac.8b00470
• Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
  Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
  Commun Biol. 0: 0 (2018) doi: 10.1038/s42003-018-0222-4
• Kanamori T, Sugiura S, Sakai Y
  Technical aspects of microphysiological systems (MPS) as a promising wet human-in-vivo simulator
  Drug Metab Pharmacokinet. 33: 40-42 (2018) doi: 10.1016/j.dmpk.2017.11.006
• Sumaru K, Takagi T, Satoh T, Kanamori T
  Photo- and Thermoresponsive Dehydration of Spiropyran-Functionalized Polymer Regulated by Molecular Recognition
  Macromol Rapid Commun. 39: 0 (2018) doi: 10.1002/marc.201700234
• Ito N, Katoh K, Kushige H, Saito Y, Umemoto T, Matsuzaki Y, Kiyonari H, Kobayashi D, Soga M, Era T, Araki N, Furuta Y, Suda T, Kida Y, Ohta K^*
  Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency
  Sci Rep. 8: 1634 (2018) doi: 10.1038/s41598-018-20057-1
• Ueyama T, Nakao S, Tsukamoto T, Ihara D, Harada Y, Akagi Y, Nakagawa S, Kida YS, Sogo T and Kawamura T^*
  PTEN/Akt Axis is Involved in Somatic Cell Reprogramming to Mouse iPS Cells
  Biomed J Sci & Tech. Res. 11: 5 (2018) DOI: 10.26717/BJSTR.2018.11.002162
• Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
  Study of Perfluoroalkyl Chain-Specific Band Shift in Infrared Spectra on the Chain Length
  J. Physical Chemistry A 121: 8425-8431 (2017) doi: 10.1021/acs.jpca.7b08940
• Kondo Y, Hattori K, Tashiro S, Nakatani E, Yoshimitsu R, Satoh T, Sugiura S, Kanamori T, Ohnuma K
  Compartmentalized microfluidic perfusion system to culture human induced pluripotent stem cell aggregates
  J Biosci Bioeng. 124: 234-241 (2017) doi: 10.1016/j.jbiosc.2017.03.014
• Sumaru K, Morishita K, Takagi T, Satoh T, Kanamori T
  Sectioning of cultured cell monolayer using photo-acid-generating substrate and micro-patterned light projection
  European Polymer J. 121: 16964-16969 (2017) doi: 10.1016/j.eurpolymj.2017.02.021
• Watanabe Y, Miyasaka KY, Kubo A, Kida YS, Nakagawa O, Hirate Y, Sasaki H, Ogura T^*
  Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm
  Sci Rep. 0: 0 (2017) doi: 10.1038/srep46135
• Kondo Y, Yada Y, Haga T, Takayama Y, Isomura T, Jimbo Y, Fukayama O, Hoshino T^*, Mabuchi K
  Temporal relation between neural activity and neurite pruning on a numerical model and a microchannel device with micro electrode array
  Biochem Biophys Res Commun. 486: 539-544 (2017) doi: 10.1016/j.bbrc.2017.03.082
• Tamura M, Sugiura S, Takagi T, Satoh T, Sumaru K, Kanamori T, Okada T, Matsui H
  Morphology-based optical separation of subpopulations from a heterogeneous murine breast cancer cell line
  PloS One. 12: 0 (2017) doi: 10.1371/journal.pone.0179372
• Takayama Y^#, Wakabayashi T#, Kushige H, Saito Y, Shibuya Y, Shibata S, Akamatsu W, Okano H, Kida YS^*
  Brief Exposure to Small Molecules Allows Induction of Mouse Embryonic Fibroblasts Into Neural Crest-Like Precursors
  FEBS Lett. 591: 590-602 (2017) doi: 10.1002/1873-3468.12572
• Satoh T^#, Sugiura S^#*, Shin K, Onuki-Nagasaki R, Ishida S, Kikuchi K, Kakiki M, Kanamori T
  A Multi-Throughput Multi-Organ-On-A-Chip System on a Plate Formatted Pneumatic Pressure-Driven Medium Circulation Platform
  Lab Chip. 18:115-125 (2017) doi: 10.1039/c7lc00952f