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Applied Molecular Function Research Group

Project

Project1:Molecular technology to manipulate cell culture
Researcher:Kimio Sumaru, Toshiyuki Takagi, Teruhiko Baba, Masayuki Sano

We have developed new elemental technologies to manipulate cell cultures using photoresponsive polymers, fluorinated lipids, membrane proteins and RNA vectors capable of stable and persistent gene expression.

Applied Molecular Function Research Group02

Project

Project2:Production and analysis of glycoproteins
Researcher:Akihiko Kameyama, Masahiro Asada, Takehiko Yoko-o, Yasunori Chiba

We have developed new technologies to analyze huge glycoproteins, to mass-produce glycoproteins using newly established yeasts, and to functionalize proteins via glycosylation to enhance their stability and activity in living systems.

Applied Molecular Function Research Group03

Member

Name Position
SUMARU Kimio Research Group Leader
KAMEYAMA Akihiko Chief Senior Researcher
BABA Teruhiko Senior Researcher
ASADA Masahiro Senior Researcher
YOKO-O Takehiko Senior Researcher
TAKAGI Toshiyuki Senior Researcher
SANO Masayuki Senior Researcher

Results

  • Matsuzawa T, Kameyama A, Nakamichi Y, Yaoi K
    Identification and characterization of two xyloglucan-specific endo-1,4-glucanases in Aspergillus oryzae.
    Appl Microbiol Biotechnol. 2020 Sep 10. (2020) doi: 10.1007/s00253-020-10883-7
  • Abe T, Kameyama A, Natsuka S, Suzuki N
    Sequential modifications of glycans by linkage-specific alkylamidation of sialic acids and permethylation.
    Anal Biochem. 606:113861. (2020) doi: 10.1016/j.ab.2020.113861
  • Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
    Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
    ROYAL SOCIETY OPEN SCIENCE 7 (7); (2020) doi: 10.1098/rsos.200027
  • Akintayo A, Mayoral J, Asada M, Tang J, Sundaram S, Stanley P
    Point Mutations that Inactivate MGAT4D-L, an Inhibitor of MGAT1 and Complex N-Glycan Synthesis
    J Biol Chem. :jbc.RA120.014784. (2020) doi: 10.1074/jbc.RA120.014784
  • Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
    Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
    Royal Society Open Science 7 7: 0 (2020) doi:10.1098/rsos.200027
  • Watanabe U,Sugiura S, Kakehata M, Yanagawa F, Takagi T, Sumaru K, Satoh T, Tamura M, Hosokawa Y, Torizuka K, Kanamori T
    Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering
    Micromachines 11: 0 (2020) doi:10.3390/mi11070679
  • Huang GL, Li Z, Li YQ, Liu G, Sun SJ, Gu JG, Kameyama A, Li WZ, Dong WJ
    Loss of core fucosylation in both ST6GAL1 and its substrate enhances glycoprotein sialylation in mice
    Biochem. J. 477 477: 1179-1201 (2020) doi: 10.1042/BCJ20190789
  • Baba T, Takagi T, Sumaru K, Kanamori T
    Effect of the fluorination degree of partially fluorinated octyl-phosphocholine surfactants on their interfacial properties and interactions with purple membrane as a membrane protein model
    Chemistry And Physics Lipids 227 0: 0 (2020) doi: 10.1016/j.chemphyslip.2020.104870
  • Matsuzawa T, Kameyama A, Yaoi K
    Identification and characterization of alpha-xylosidase involved in xyloglucan degradation in Aspergillus oryzae
    Applied Microbiology And Biotechnology 104 104: 201-210 (2020) doi: 10.1007/s00253-019-10244-z
  • Sano M, Nakasu A, Ohtaka M, Nakanishi M
    A Sendai Virus-Based Cytoplasmic RNA Vector as a Novel Platform for Long-Term
    Expression of MicroRNAs
    Mol Ther Methods Clin Dev. 15: 371-382 (2019) doi: 10.1016/j.omtm.2019.10.012
  • Tsuyukubo A, Sumaru K, Kanamori T
    Photolithographic Fabrication of Semi 3-D Microstructures Composed of Flexible Hydrogel Sheet for in Vivo-like Cell Culture System
    ACS App Bio Mater. 2: 4129–4133 (2019) doi: 10.1021/acsabm.9b00656,
  • Komatsu H, Sakai K, Baba T, Takagi T, Kanamori T
    Aggregation behavior of short-chained archaeal phospholipid analogs: Contribution methyl branches to lipid hydrophobicity and membrane formability
    Colloid And Interface Science Communications 129: 3827-3832 (2019) doi: 10.1016/j.colcom.2019.100200
  • Morita K, Horikoshi M, Yanagi T, Takagi T, Takahashi H, Amii H, Hasegawa T, Sonoyama M
    Thermotropic Transition Behaviors of Novel Partially Fluorinated Dimyristoylphosphatidylcholines with Different Perfluoroalkyl Chain Lengths
    Chemistry Letters 48: 1105-1108 (2019) doi: 10.1246/cl.190389
  • Mizuguchi C, Nakagawa M, Namba N, Sakai M, Kurimitsu N, Suzuki A, Fujita K, Horiuchi S, Baba T, Ohgita T, Nishitsuji K, Saito H
    Mechanisms of aggregation and fibril formation of the amyloidogenic N-terminal fragment of apolipoprotein A-I
    J Biol Chem. 294: 13515-13524 (2019) doi: 10.1074/jbc.RA119.008000
  • Matsuzawa T, Watanabe M, Kameda T, Kameyama A, Yaoi K
    Cooperation between beta-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae
    FEBS JOURNAL 286: 3182-3193 (2019) doi: 10.1111/febs.14848
  • Kameyama A
    Towards a Novel Platform for Mucin Analysis
    Trends In Glycoscience And Glycotechnology 31: 0 (2019) doi: 10.4052/tigg.1918.2SE
  • Zhao SB, Suda Y, Nakanishi H, Wang N, Yoko-O T, Gao XD, Fujita M
    Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network
    Biochim Biophys Acta Gen Subj. 1863: 1147-1157 (2019) doi: 10.1016/j.bbagen.2019.04.009
  • Kameyama A, Thet Tin WW, Toyoda M, Sakaguchi M
    A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase
    Biochem Biophys Res Commun. 513: 186-192 (2019) doi: 10.1016/j.bbrc.2019.03.144
  • Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
    Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center
    J. Physical Chemistry A 123: 3985-3991 (2019) doi: 10.1021/acs.jpca.8b11613
  • Yanagi T, Takagi T, Takahashi H, Kikukawa T, Amii H, Sonoyama M
    Comparison of two different partially fluorinated phosphatidylcholines with the perfluorobutyl group on thermotropic properties of the bilayer membrane and reconstituted bacteriorhodopsin
    Biophys Rev. 6749: 30628-1 (2019) doi: 10.1007/s12551-019-00540-3
  • Xu J, Sun HB, Huang GL, Liu G, Li Z, Yang H, Jin LL, Cui XL, , Shi L, Ma TH, Kameyama A, Dong WJ
    A fixation method for the optimisation of western blotting
    Scientific Reports 116: 8950-8959 (2019) doi: 10.1038/s41598-019-43039-3
  • Kise R, Fukumi A, Shioya N, Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Eda K, Hasegawa T
    Fluorous Property of a Short Perfluoroalkyl-Containing Compound Realized by Self-Assembled Monolayer Technique on a Silicon Substrate
    Bulletin The Chemical Society Japan 92: 785-789 (2019) doi: 10.1246/bcsj.20180356
  • Takahashi H, Yoshino M, Morita K, Takagi T, Yokoyama Y, Kikukawa T, Amii H, Kanamori T, Sonoyama M
    Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
    Biochim Biophys Acta Biomembr. 1861: 631-642 (2019) doi: 10.1016/j.bbamem.2018.12.015
  • Isaji T, Im S, Kameyama A, Wang Y, Fukuda T, Gu J
    A complex between phosphatidylinositol 4-kinase IIalpha and integrin alpha3beta1 is required for N-glycan sialylation in cancer cells
    J Biol Chem. 294: 4425-4436 (2019) doi: 10.1074/jbc.RA118.005208
  • Yoko-o T, Komatsuzaki A, Yoshihara E, Umemura M, Chiba Y
    Mating type switching, formation of diploids, and sporulation in the methylotrophic yeast Ogataea minuta J. Bioscience And Bioengineering 127: 1-7 (2019) doi: 10.1016/j.jbiosc.2018.07.008
  • Kameyama A, Yamakoshi K, Watanabe A
    A rapid separation and characterization of mucins from mouse submandibular glands by supported molecular matrix electrophoresis
    Biochimica Et Biophysica Acta-Proteins And Proteomics 1867: 76-81 (2019) doi: 10.1016/j.bbapap.2018.05.006
  • Iida M, Matsuno YK, Watanabe A, Maruyama M, Kameyama A, Yamakoshi K
    A sialo-oligosaccharide-rich mucin-like molecule specifically detected in the submandibular glands of aged mice
    Arch Oral Biol. 34: 89-94 (2019) doi: 10.1016/j.archoralbio.2018.10.011
  • Matsuzawa T, Kameyama A, Yaoi K
    A novel isoprimeverose-producing enzyme from Phaeoacremonium minimum is active with low concentrations of xyloglucan oligosaccharides
    FEBS Open Bio. 9: 92-100 (2019) doi: 10.1002/2211-5463.12549
  • Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
    Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
    Commun Biol. 1: 218 (2018) doi: 10.1038/s42003-018-0222-4
  • Shibuta M, Tamura M, Kanie K, Yanagisawa M, Matsui H, Satoh T, , Takagi T, Kanamori T, Sugiura S, Kato R
    Imaging cell picker: A morphology-based automated cell separation system on a photodegradable hydrogel culture platform
    J. Bioscience And Bioengineering 126: 653-660 (2018) doi: 10.1016/j.jbiosc.2018.05.004
  • Xu XX, Li ST, Wang N, Kitajima T, Yoko-O T, Fujita M, Nakanishi H, Gao XD
    Structural and functional analysis of Alg1 beta-1,4 mannosyltransferase reveals the physiological importance of its membrane topology
    Glycobiology. 28: 741-753 (2018) doi: 10.1093/glycob/cwy060
  • Yoko-O T, Umemura M, Komatsuzaki A, Ikeda K, Ichikawa D, Takase K, Kanzawa N, Saito K, Kinoshita T, Taguchi R, Jigami Y
    Lipid moiety of glycosylphosphatidylinositol-anchored proteins contributes to the determination of their final destination in yeast
    Genes Cells. 23: 880-892 (2018) doi: 10.1111/gtc.12636
  • Sumaru K, Takagi T, Morishita K, Satoh T, Kanamori T
    Fabrication of pocket-like hydrogel microstructures through photolithography
    Soft Matter 14: 5710-5714 (2018) doi: 10.1039/c8sm00865e
  • Sumaru K, Takagi T, Morishita K, Morishita K, Satoh T, Kanamori T
    Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
    Biomacromolecules. 19: 2913-2922 (2018) doi: 10.1021/acs.biomac.8b00470
  • Mizuguchi C, Nakamura M, Kurimitsu N, Ohgita T, Nishitsuji K, Baba T, Shigenaga A, Shimanouchi T, Okuhira K, Otaka A, Saito H
    Effect of Phosphatidylserine and Cholesterol on Membrane-mediated Fibril Formation by the N-terminal Amyloidogenic Fragment of Apolipoprotein A-I
    Sci Rep. 8: 5497 (2018) doi: 10.1038/s41598-018-23920-3
  • Kameyama A, Dissanayake SK, Thet Tin WW
    Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans
    PloS One. 13: 0 (2018) doi: 10.1371/journal.pone.0196800
  • Xu XX, Komatsuzaki A, Chiba Y, Gao XD, Yoko-O T
    PER1, GUP1 and CWH43 of methylotrophic yeast Ogataea minuta are involved in cell wall integrity
    Yeast. 35: 225-236 (2018) doi: 10.1002/yea.3285
  • Sumaru K, Takagi T, Satoh T, Kanamori T
    Photo- and Thermoresponsive Dehydration of Spiropyran-Functionalized Polymer Regulated by Molecular Recognition
    Macromol Rapid Commun. 39: 0 (2018) doi: 10.1002/marc.201700234
  • Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
    Study of Perfluoroalkyl Chain-Specific Band Shift in Infrared Spectra on the Chain Length
    J. Physical Chemistry A 121: 8425-8431 (2017) doi: 10.1021/acs.jpca.7b08940
  • Sano M, Ohtaka M, Iijima M, Nakasu A, Kato Y, Nakanishi M
    Sensitive and long-term monitoring of intracellular microRNAs using a non-integrating cytoplasmic RNA vector
    Sci Rep. 7: 12673 (2017) doi: 10.1038/s41598-017-12847-w
  • Zhou Y, Fukuda T, Hang Q, Hou S, Isaji T, Kameyama A, Gu J
    Inhibition of fucosylation by 2-fluorofucose suppresses human liver cancer HepG2 cell proliferation and migration as well as tumor formation
    Sci Rep. 7: 11563 (2017) doi: 10.1038/s41598-017-11911-9
  • Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M
    Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302
    Stem Cell Res. 23: 13-19 (2017) doi: 10.1016/j.scr.2017.06.011
  • Sumaru K, Morishita K, Takagi T, Satoh T, Kanamori T
    Sectioning of cultured cell monolayer using photo-acid-generating substrate and micro-patterned light projection
    European Polymer J. 121: 16964-16969 (2017) doi: 10.1016/j.eurpolymj.2017.02.021
  • Tamura M, Sugiura S, Takagi T, Satoh T, Sumaru K, Kanamori T, Okada T, Matsui H
    Morphology-based optical separation of subpopulations from a heterogeneous murine breast cancer cell line
    PloS One. 12: 0 (2017) doi: 10.1371/journal.pone.0179372
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