Applied Molecular Function Research Group

Research Project

We have developed technologies for sequencing, sorting, and designing cultured cells as desired using functional polymers; for cell reprogramming and functional expression analysis using sustained expression RNA vectors; and for fluorinated lipids, which are expected to be deployed for functionalization of cells.

Research Project

We have developed a new membrane electrophoresis method that enables precise analysis of mucins, which are giant glycoproteins. We have also created special yeast and cell lines and developed a technology to produce useful glycoproteins using these yeast and cells.

Research Project

Molecular functions generated by the water-biomolecule system involve the heterogeneity of water dynamics and slow relaxation processes due to hydration. Based on the observation and control of the hydration, we are developing technologies with broad applications across various fields, including biotechnology. We have revealed that sub-THz waves can be utilized for this control.

Results

• Kurosu, J; Sakamaki, T; Kanai, K; Morishita, K; Sumaru, K; Tsutsumi, J.
  Spatiotemporal dynamics of microscopic biological barrier visualized by electric-double-layer modulation imaging.
  BIOSENS BIOELECTRON. 2024 Aug 30;266:116721. doi: 10.1016/j.bios.2024.116721
• Inuzuka, N; Shobayashi, Y; Tateshima, S; Sato, Y; Ohba, Y; Ishihara, K; Teramura, Y.
  Stable and Thin-Polymer-Based Modification of Neurovascular Stents with 2-Methacryloyloxyethyl Phosphorylcholine Polymer for Antithrombogenicity.
  BIOENGINEERING (Basel). 2024 Aug 15;11(8):833. doi: 10.3390/bioengineering11080833
• Sato, Y; Baba, T; Uchida, T; Chung, UI; Teramura, Y.
  Cell membrane fusion induced by surface modification with cell-penetrating peptide–lipid conjugates that facilitates close contact between distinct membranes.
  Mater. Adv. 2024 May 11. doi: 10.1039/D4MA00193A
• Sato, Y; Zhang, WX; Baba, T; Chung, UI; Teramura, Y.
  Extracellular vesicle-liposome hybrids via membrane fusion using cell-penetrating peptide-conjugated lipids.
  REGENERATIVE THERAPY. 2024 Jul 29. doi: 10.1016/j.reth.2024.07.006
• Ma, ST; Yoda, H; Dang, T; Yang, JQ; Ando, I; Sato, Y; Teramura, Y; Tabata, H; Matsui, H.
  Light Polarization Effects of Chemical Sensing in the Mid-Infrared Region Using ZnO: Ga-Based Plasmonic Surface Lattice Resonances.
  JOURNAL OF PHYSICAL CHEMISTRY C. July 15, 2024. doi: 10.1021/acs.jpcc.4c02354
• Inuzuka, N; Shobayashi, Y; Tateshima, S; Sato, Y; Ohba, Y; Ekdahl, KN; Nilsson, B; Teramura, Y.
  Stent coating containing a charged silane coupling agent that regulates protein adsorption to confer antithrombotic and cell-adhesion properties.
  SCI REP. 2024 Jul 10;14(1):15178. doi: 10.1038/s41598-024-65832-5
• Shu, Y; Otake, M; Seta, Y; Hori, K; Kuramochi, A; Ohba, Y; Teramura, Y.
  Activation of cellular antioxidative stress and migration activities by purified components from immortalized stem cells from human exfoliated deciduous teeth.
  SCI REP. 2024 Jul 3;14(1):15340. doi: 10.1038/s41598-024-66213-8
• Shimada, N; Kameyama, A; Watanabe, M; Sahara, T; Matsuzawa, T.
  Identification and characterization of xyloglucan-degradation related α-1,2-l-fucosidase in Aspergillus oryzae.
  J BIOSCI BIOENG. 2024 Jun 12:S1389-1723(24)00159-2. doi: 10.1016/j.jbiosc.2024.05.011
• Qayyum, MZ; Imashimizu, M; Leanca, M; Vishwakarma, RK; Riaz-Bradley, A; Yuzenkova, Y; Murakami, KS.
  Structure and function of the Si3 insertion integrated into the trigger loop/helix of cyanobacterial RNA polymerase.
  PROC NATL ACAD SCI U S A. 2024 Feb 20;121(8):e2311480121. doi: 10.1073/pnas.2311480121
• Dong, W; Kameyama, A.
  Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membrane.
  METHODS MOL BIOL. 2024;2763:111-117. doi: 10.1007/978-1-0716-3670-1_9
• Kameyama, A.
  Eliminative Oximation of O-Glycans from Mucins.
  METHODS MOL BIOL. 2024;2763:151-158. doi: 10.1007/978-1-0716-3670-1_13
• Kameyama, A.
  Supported Molecular Matrix Electrophoresis.
  METHODS MOL BIOL. 2024;2763:79-97. doi: 10.1007/978-1-0716-3670-1_7
• Sugiura, T; Kameyama, A.
  Preparation of Soluble Mucin Solutions from the Salivary Glands.
  METHODS MOL BIOL. 2024;2763:45-50. doi: 10.1007/978-1-0716-3670-1_3
• Hasegawa, T; Nakagawara, A; Takagi, T; Shimoaka, T; Shioya, N; Sonoyama, M.
  Phonon modes controlled by primary chemical structure of partially fluorinated dimyristoylphosphatidylcholine (DMPC) revealed by multiple-angle incidence resolution spectrometry (MAIRS).
  J CHEM PHYS. 2024 Feb 14;160(6):064704. doi: 10.1063/5.0190441
• Kishimoto, T; Nishimura, K; Morishita, K; Fukuda, A; Miyamae, Y; Kumagai, Y; Sumaru, K; Nakanishi, M; Hisatake, K; Sano, M.
  An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors.
  J BIOL ENG. 2024 Jan 16;18(1):9. doi: 10.1186/s13036-024-00404-9
• Miyazaki, M; Arisaka, C; Nakagawara, A; Sasaki, N; Takahashi, H; Takagi, T; Amii, H; Sonoyama, M.
  Thermodynamic study on hydrated bilayers of ether-linked phosphatidylcholines with terminal perfluorobutyl group.
  BIOCHIM BIOPHYS ACTA BIOMEMBR. 2023 Dec 13:184261. doi: 10.1016/j.bbamem.2023.184261
• Adler, A; Fritsch, M; Fromell, K; Leneweit, G; Ekdahl, KN; Nilsson, B; Teramura, Y.
  Regulation of the innate immune system by fragmented heparin-conjugated lipids on lipid bilayered membranes in vitro.
  J MATER CHEM B. 2023 Nov 13. doi: 10.1039/d3tb01721d
• Takeda, N; Tsuchiya, A; Mito, M; Natsui, K; Natusi, Y; Koseki, Y; Tomiyoshi, K; Yamazaki, F; Yoshida, Y; Abe, H; Sano, M; Kido, T; Yoshioka, Y; Kikuta, J; Itoh, T; Nishimura, K; Ishii, M; Ochiya, T; Miyajima, A; Terai, S.
  Analysis of distribution, collection, and confirmation of capacity dependency of small extracellular vesicles toward a therapy for liver cirrhosis.
  INFLAMM REGEN. 2023 Oct 9;43(1):48. doi: 10.1186/s41232-023-00299-x
• Taniguchi, M; Okumura, R; Matsuzaki, T; Nakatani, A; Sakaki, K; Okamoto, S; Ishibashi, A; Tani, H; Horikiri, M; Kobayashi, N; Yoshikawa, HY; Motooka, D; Okuzaki, D; Nakamura, S; Kida, T; Kameyama, A, Takeda, K.
  Sialylation shapes mucus architecture inhibiting bacterial invasion in the colon.
  MUCOSAL IMMUNOL. 2023 Jun 27:S1933-0219(23)00049-1. doi: 10.1016/j.mucimm.2023.06.004
• Tsuyukubo, A; Morishita, K; Kanamori, T; Sumaru, K.
  Construction of a spheroid array culture system on a suspended permeable hydrogel membrane scaffold for improving the expression of a liver-specific drug-metabolizing enzyme of HepG2 cells.
  BIOMATER SCI. 2023 Jun 22. doi: 10.1039/d3bm00071k
• Sugiyama, JI; Tokunaga, Y; Hishida, M; Tanaka, M; Takeuchi, K; Satoh, D; Imashimizu, M.
  Nonthermal acceleration of protein hydration by sub-terahertz irradiation.
  NAT COMMUN. 2023 May 22;14(1):2825. doi: 10.1038/s41467-023-38462-0
• Nakagawa, Y; Hishida, T; Sumaru, K; Morishita, K; Kirito, K; Yokojima, S; Sakamoto, Y; Nakamura, S; Uchida, K.
  Phototunable Cell Killing by Photochromic Diarylethene of Thiazoyl and Thienyl Derivatives.
  JOURNAL OF MEDICINAL CHEMISTRY. 2023, 66 (8) 5937-5949. doi: 10.1021/acs.jmedchem.3c00164
• Sugiura, T; Hashimoto, K; Kikuta, K; Anazawa, U; Nomura, T; Kameyama, A.
  Expression and localisation of MUC1 modified with sialylated core-2 O-glycans in mucoepidermoid carcinoma.
  SCI REP. 2023 Apr 8;13(1):5752. doi: 10.1038/s41598-023-32597-2
• Rangasami, VK; Asawa, K; Teramura, Y; Le, Blanc K; Nilsson, B; Hilborn, J; Varghese, OP; Oommen, OP.
  Biomimetic polyelectrolyte coating of stem cells suppresses thrombotic activation and enhances its survival and function.
  BIOMATER ADV. 2023 Feb 8;147:213331. doi: 10.1016/j.bioadv.2023.213331
• Huang, T; Sato, Y; Kuramochi, A; Ohba, Y; Sano, M; Miyagishi, M; Tateno, H; Wadhwa, R; Kawasaki, K; Uchida, T; Ekdahl, KN; Nilsson, B; Chung, UI; Teramura, Y.
  Surface modulation of extracellular vesicles with cell-penetrating peptide-conjugated lipids for improvement of intracellular delivery to endothelial cells.
  REGEN THER. 2023 Jan 11;22:90-98. doi: 10.1016/j.reth.2022.12.007
• Tsuda, M; Nakatani, Y; Baba, S; Tanaka, I; Ichikawa, K; Nonaka, K; Ito, R; Yoko-O, T; Chiba, Y.
  Selection of the methylotrophic yeast Ogataea minuta as a high-producing host for heterologous protein expression.
  J BIOSCI BIOENG. 2023 Jan 24:S1389-1723(22)00375-9. doi: 10.1016/j.jbiosc.2022.12.006
• Suzuki, H; Adler, A; Huang, T; Kuramochi, A; Ohba, Y; Sato, Y; Nakamura, N; Manivel, VA; Ekdahl, KN; Nilsson, B; Ishihara, K; Teramura, Y.
  Impact of spontaneous liposome modification with phospholipid polymer-lipid conjugates on protein interactions.
  SCI TECHNOL ADV MATER. 2022 Dec 8;23(1):845-857. doi: 10.1080/14686996.2022.2146466
• Ekdahl, KN; Fromell, K; Mannes, M; Grinnemo, KH; Huber-Lang, M;Teramura, Y; Nilsson, B.
  Therapeutic regulation of complement activation in extracorporeal circuits and intravascular treatments with special reference to the alternative pathway amplification loop.
  IMMUNOL REV. 2022 Oct 18. doi: 10.1111/imr.13148
• Sano, M; Morishita, K; Onizawa, Y; Takagi, T; Sumaru, K.
  Rapid and Highly Sensitive Method for Evaluating Surface Coatings against an Enveloped RNA Virus.
  ACS APPL BIO MATER. 2022 Oct 14. doi: 10.1021/acsabm.2c00613
• Mellul, M; Lahav, S; Imashimizu, M; Tokunaga, Y; Lukatsky, DB; Ram, O.
  Repetitive DNA symmetry elements negatively regulate gene expression in embryonic stem cells.
  BIOPHYS J. 2022 Jul 8:S0006-3495(22)00557-4. doi: 10.1016/j.bpj.2022.07.011
• Omoto, T; Yimiti, D; Sanada, Y; Toriyama, M; Ding, C; Hayashi, Y; Ikuta, Y; Nakasa, T; Ishikawa, M; Sano, M; Lee, M; Akimoto, T; Shukunami, C; Miyaki, S; Adachi, N.
  Tendon-Specific Dicer Deficient Mice Exhibit Hypoplastic Tendon Through the Downregulation of Tendon-Related Genes and MicroRNAs.
  FRONT CELL DEV BIOL. 2022 Jun 14;10:898428. doi: 10.3389/fcell.2022.898428
• Adler, A; Manivel, VA; Fromell, K; Teramura, Y; Ekdahl, KN; Nilsson, B.
  A Robust Method to Store Complement C3 With Superior Ability to Maintain the Native Structure and Function of the Protein.
  FRONT IMMUNOL. 2022 May 3;13:891994. doi: 10.3389/fimmu.2022.891994
• Nakagawa, Y; Hishida, T; Hatano, E; Sumaru, K; Morishita, K; Morimoto, M; Yokojima, S; Nakamura, S; Uchida, K.
  Photoinduced cytotoxicity of photochromic symmetric diarylethene derivatives: the relation of structure and cytotoxicity.
  ORGANIC & BIOMOLECULAR CHEMISTRY. 2022 Apr 13;20(15):3211-3217. doi: 10.1039/d2ob00224h
• Sogabe, M; Kojima, S; Kaya, T; Tomioka, A; Kaji, H; Sato, T; Chiba, Y; Shimizu, A; Tanaka, N; Suzuki, N; Hayashi, I; Mikami, M; Togayachi, A; Narimatsu, H.
  Sensitive New Assay System for Serum Wisteria floribunda Agglutinin-Reactive Ceruloplasmin That Distinguishes Ovarian Clear Cell Carcinoma from Endometrioma.
  ANALYTICAL CHEMISTRY; doi:10.1021/acs.analchem.1c04302
• Goel, I; Noiri, M; Yamauchi, Y; Kato, K; Chung, UI; Teramura, Y.
  Enhancement of intercellular interaction between iPSC-derived neural progenitor cells and activated endothelial cells using cell surface modification with functional oligopeptides.
  BIOMATER SCI. 2022 Jan 11. doi: 10.1039/d1bm01503f
• Matsuzawa, T; Watanabe, M; Nakamichi, Y; Kameyama, A; Kojima, N; Yaoi, K.
  Characterization of an extracellular α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae.
  APPL MICROBIOL BIOTECHNOL. 2021 Dec 31. doi: 10.1007/s00253-021-11744-7
• Hurd, MD; Goel, I; Sakai, Y; Teramura, Y.
  Current status of ischemic stroke treatment: From thrombolysis to potential regenerative medicine.
  REGENERATIVE THERAPY 18 408-417; doi:10.1016/j.reth.2021.09.009 DEC
• Adler, A; Inoue, Y; Ekdahl, KN; Baba, T; Ishihara, K; Nilsson, B; Teramura, Y.
  Effect of liposome surface modification with water-soluble phospholipid polymer chain-conjugated lipids on interaction with human plasma proteins.
  J MATER CHEM B. 2021 Oct 7. doi: 10.1039/d1tb01485d
• Sano, M; Morishita, K; Oikawa, S; Akimoto, T; Sumaru, K; Kato, Y.
  Live-cell imaging of microRNA expression with post-transcriptional feedback control.
  MOL THER NUCLEIC ACIDS. 2021 Aug 26;26:547-556. doi: 10.1016/j.omtn.2021.08.018
• Yoko-O, T; Komatsuzaki, A; Yoshihara, E; Zhao, S; Umemura, M; Gao, XD; Chiba, Y.
  Regulation of alcohol oxidase gene expression in methylotrophic yeast Ogataea minuta.
  J BIOSCI BIOENG. 2021 Aug 27:S1389-1723(21)00204-8. doi: 10.1016/j.jbiosc.2021.08.001
• Isaka, E; Sugiura, T; Hashimoto, K; Kikuta, K; Anazawa, U; Nomura, T; Kameyama, A.
  Characterization of tumor-associated MUC1 and its glycans expressed in mucoepidermoid carcinoma.
  ONCOLOGY LETTERS 22 (4);doi: 10.3892/ol.2021.12963 OCT
• Sato, Y; Asawa, K; Huang, T; Noiri, M; Nakamura, N; Ekdahl, KN; Nilsson, B; Ishihara, K; Teramura, Y.
  Induction of Spontaneous Liposome Adsorption by Exogenous Surface Modification with Cell-Penetrating Peptide-Conjugated Lipids.
  LANGMUIR. 2021 Aug 3. doi: 10.1021/acs.langmuir.1c01072
• Adler, A; Inoue, Y; Sato, Y; Ishihara, K; Ekdahl, KN; Nilsson, B; Teramura, Y.
  Synthesis of poly(2-methacryloyloxyethyl phosphorylcholine)-conjugated lipids and their characterization and surface properties of modified liposomes for protein interactions.
  BIOMATER SCI. 2021 Jul 20. doi: 10.1039/d1bm00570g
• Hashimoto, M; Murai, Y; Morita, K; Kikukawa, T; Takagi, T; Takahashi, H; Yokoyama, Y; Amii, H; Sonoyama, M.
  Comparison of functionality and structural stability of bacteriorhodopsin reconstituted in partially fluorinated dimyristoylphosphatidylcholine liposomes with different perfluoroalkyl chain lengths.
  BIOCHIM BIOPHYS ACTA BIOMEMBR. 2021 Jun 25;1863(10):183686. doi: 10.1016/j.bbamem.2021.183686
• Tsuchida N, Takagi T, Takahashi H, Yoshihara T, Tobita S, Sonoyama M
  Membrane properties of amacrocyclic tetraether bisphosphatidylcholine lipid: Effect of a single membrane-spanning polymethylene cross-linkage between two head groups of ditetradecylphosphatidylcholine membrane.
  BIOCHIM BIOPHYS ACTA BIOMEMBR. 2021 Feb 4:183569. doi: 10.1016/j.bbamem.2021.183569
• Kameyama A, Nishijima R, Yamakoshi K
  Bmi-1 regulates mucin levels and mucin O-glycosylation in the submandibular gland of mice.
  PLOS ONE. 2021 Jan 19;16(1):e0245607. doi: 10.1371/journal.pone.0245607. eCollection
• Liu D, Liu G, Li Y, Wang Y, Zheng Y, Sha S, Li W, Kameyama A, Dong W
  Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.
  J PROTEOMICS. 2021 Jan 6:104098. doi: 10.1016/j.jprot.2020.104098
• Baba T, Takagi T, Sumaru K, Kanamori T, Dewa T, Nango M
  Membrane properties of ether-type phosphatidylcholine bearing partially fluorinated C(18)-monoacetylenic chains and their applicability to membrane protein reconstitution matrices
  Colloids Surf B Biointerfaces. 2020 Nov 13:111459. doi: 10.1016/j.colsurfb.2020.111459
• Kameyama A, Thet Tin WW, Nishijima R, Yamakoshi K
  Alteration of mucins in the submandibular gland during aging in mice.
  ARCH ORAL BIOL.121:104967 (2020) doi: 10.1016/j.archoralbio.2020.104967
• Matsuzawa T, Kameyama A, Nakamichi Y, Yaoi K
  Identification and characterization of two xyloglucan-specific endo-1,4-glucanases in Aspergillus oryzae.
  Appl Microbiol Biotechnol. 2020 Sep 10. (2020) doi: 10.1007/s00253-020-10883-7
• Abe T, Kameyama A, Natsuka S, Suzuki N
  Sequential modifications of glycans by linkage-specific alkylamidation of sialic acids and permethylation.
  Anal Biochem. 606:113861. (2020) doi: 10.1016/j.ab.2020.113861
• Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
  Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
  ROYAL SOCIETY OPEN SCIENCE 7 (7); (2020) doi: 10.1098/rsos.200027
• Akintayo A, Mayoral J, Asada M, Tang J, Sundaram S, Stanley P
  Point Mutations that Inactivate MGAT4D-L, an Inhibitor of MGAT1 and Complex N-Glycan Synthesis
  J Biol Chem. :jbc.RA120.014784. (2020) doi: 10.1074/jbc.RA120.014784
• Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
  Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
  Royal Society Open Science 7 7: 0 (2020) doi:10.1098/rsos.200027
• Watanabe U,Sugiura S, Kakehata M, Yanagawa F, Takagi T, Sumaru K, Satoh T, Tamura M, Hosokawa Y, Torizuka K, Kanamori T
  Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering
  Micromachines 11: 0 (2020) doi:10.3390/mi11070679
• Huang GL, Li Z, Li YQ, Liu G, Sun SJ, Gu JG, Kameyama A, Li WZ, Dong WJ
  Loss of core fucosylation in both ST6GAL1 and its substrate enhances glycoprotein sialylation in mice
  Biochem. J. 477 477: 1179-1201 (2020) doi: 10.1042/BCJ20190789
• Baba T, Takagi T, Sumaru K, Kanamori T
  Effect of the fluorination degree of partially fluorinated octyl-phosphocholine surfactants on their interfacial properties and interactions with purple membrane as a membrane protein model
  Chemistry And Physics Lipids 227 0: 0 (2020) doi: 10.1016/j.chemphyslip.2020.104870
• Matsuzawa T, Kameyama A, Yaoi K
  Identification and characterization of alpha-xylosidase involved in xyloglucan degradation in Aspergillus oryzae
  Applied Microbiology And Biotechnology 104 104: 201-210 (2020) doi: 10.1007/s00253-019-10244-z
• Sano M, Nakasu A, Ohtaka M, Nakanishi M
  A Sendai Virus-Based Cytoplasmic RNA Vector as a Novel Platform for Long-Term
  Expression of MicroRNAs
  Mol Ther Methods Clin Dev. 15: 371-382 (2019) doi: 10.1016/j.omtm.2019.10.012
• Tsuyukubo A, Sumaru K, Kanamori T
  Photolithographic Fabrication of Semi 3-D Microstructures Composed of Flexible Hydrogel Sheet for in Vivo-like Cell Culture System
  ACS App Bio Mater. 2: 4129–4133 (2019) doi: 10.1021/acsabm.9b00656,
• Komatsu H, Sakai K, Baba T, Takagi T, Kanamori T
  Aggregation behavior of short-chained archaeal phospholipid analogs: Contribution methyl branches to lipid hydrophobicity and membrane formability
  Colloid And Interface Science Communications 129: 3827-3832 (2019) doi: 10.1016/j.colcom.2019.100200
• Morita K, Horikoshi M, Yanagi T, Takagi T, Takahashi H, Amii H, Hasegawa T, Sonoyama M
  Thermotropic Transition Behaviors of Novel Partially Fluorinated Dimyristoylphosphatidylcholines with Different Perfluoroalkyl Chain Lengths
  Chemistry Letters 48: 1105-1108 (2019) doi: 10.1246/cl.190389
• Mizuguchi C, Nakagawa M, Namba N, Sakai M, Kurimitsu N, Suzuki A, Fujita K, Horiuchi S, Baba T, Ohgita T, Nishitsuji K, Saito H
  Mechanisms of aggregation and fibril formation of the amyloidogenic N-terminal fragment of apolipoprotein A-I
  J Biol Chem. 294: 13515-13524 (2019) doi: 10.1074/jbc.RA119.008000
• Matsuzawa T, Watanabe M, Kameda T, Kameyama A, Yaoi K
  Cooperation between beta-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae
  FEBS JOURNAL 286: 3182-3193 (2019) doi: 10.1111/febs.14848
• Kameyama A
  Towards a Novel Platform for Mucin Analysis
  Trends In Glycoscience And Glycotechnology 31: 0 (2019) doi: 10.4052/tigg.1918.2SE
• Zhao SB, Suda Y, Nakanishi H, Wang N, Yoko-O T, Gao XD, Fujita M
  Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network
  Biochim Biophys Acta Gen Subj. 1863: 1147-1157 (2019) doi: 10.1016/j.bbagen.2019.04.009
• Kameyama A, Thet Tin WW, Toyoda M, Sakaguchi M
  A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase
  Biochem Biophys Res Commun. 513: 186-192 (2019) doi: 10.1016/j.bbrc.2019.03.144
• Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
  Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center
  J. Physical Chemistry A 123: 3985-3991 (2019) doi: 10.1021/acs.jpca.8b11613
• Yanagi T, Takagi T, Takahashi H, Kikukawa T, Amii H, Sonoyama M
  Comparison of two different partially fluorinated phosphatidylcholines with the perfluorobutyl group on thermotropic properties of the bilayer membrane and reconstituted bacteriorhodopsin
  Biophys Rev. 6749: 30628-1 (2019) doi: 10.1007/s12551-019-00540-3
• Xu J, Sun HB, Huang GL, Liu G, Li Z, Yang H, Jin LL, Cui XL, , Shi L, Ma TH, Kameyama A, Dong WJ
  A fixation method for the optimisation of western blotting
  Scientific Reports 116: 8950-8959 (2019) doi: 10.1038/s41598-019-43039-3
• Kise R, Fukumi A, Shioya N, Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Eda K, Hasegawa T
  Fluorous Property of a Short Perfluoroalkyl-Containing Compound Realized by Self-Assembled Monolayer Technique on a Silicon Substrate
  Bulletin The Chemical Society Japan 92: 785-789 (2019) doi: 10.1246/bcsj.20180356
• Takahashi H, Yoshino M, Morita K, Takagi T, Yokoyama Y, Kikukawa T, Amii H, Kanamori T, Sonoyama M
  Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
  Biochim Biophys Acta Biomembr. 1861: 631-642 (2019) doi: 10.1016/j.bbamem.2018.12.015
• Isaji T, Im S, Kameyama A, Wang Y, Fukuda T, Gu J
  A complex between phosphatidylinositol 4-kinase IIalpha and integrin alpha3beta1 is required for N-glycan sialylation in cancer cells
  J Biol Chem. 294: 4425-4436 (2019) doi: 10.1074/jbc.RA118.005208
• Yoko-o T, Komatsuzaki A, Yoshihara E, Umemura M, Chiba Y
  Mating type switching, formation of diploids, and sporulation in the methylotrophic yeast Ogataea minuta J. Bioscience And Bioengineering 127: 1-7 (2019) doi: 10.1016/j.jbiosc.2018.07.008
• Kameyama A, Yamakoshi K, Watanabe A
  A rapid separation and characterization of mucins from mouse submandibular glands by supported molecular matrix electrophoresis
  Biochimica Et Biophysica Acta-Proteins And Proteomics 1867: 76-81 (2019) doi: 10.1016/j.bbapap.2018.05.006
• Iida M, Matsuno YK, Watanabe A, Maruyama M, Kameyama A, Yamakoshi K
  A sialo-oligosaccharide-rich mucin-like molecule specifically detected in the submandibular glands of aged mice
  Arch Oral Biol. 34: 89-94 (2019) doi: 10.1016/j.archoralbio.2018.10.011
• Matsuzawa T, Kameyama A, Yaoi K
  A novel isoprimeverose-producing enzyme from Phaeoacremonium minimum is active with low concentrations of xyloglucan oligosaccharides
  FEBS Open Bio. 9: 92-100 (2019) doi: 10.1002/2211-5463.12549
• Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
  Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
  Commun Biol. 1: 218 (2018) doi: 10.1038/s42003-018-0222-4
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