Molecular and Cellular Glycoproteomics Research Group

Research Project

Post-translational protein modification (glycosylation, amino acid isomerization, etc.) has a pivotal role in the functional regulation of proteins via the modifications of their three-dimensional structures, subcellular localization, and interactions with other functional molecules. To better understand the biological roles of such modifications, it is important to elucidate how physiological and pathological changes will alter the synthetic machinery/mechanism, and consequently, what sites of what proteins will be modified with which structural compartments, at each level of proteins, cells, tissues, and organisms. To confront these important matters, this research group aims to develop multi-omics technologies, including glycoproteomics, glycomics, glyco-transcriptomics, and glyco-metabolomics, etc. These multi-omics data will be available in the public databases that we and our colleagues developed, which will contribute to the glycoscience community.

Research Project

Many diseases such as cancer trigger changes in cell glycomes, known as "disease-related glycosylation alteration." We have previously developed various disease-biomarkers and molecular-targeted drugs by targeting this disease-related glycosylation alteration. On the other hand, the biological significance of the phenomena is yet not well understood. Our goal is reaching to a comprehensive understanding of the physiological and pathological "function of glycans" in tumors, virus infections, aging as well as immunities, reproductions, and other cell functions by using multi-omics approaches.

Results

• Hiono, T; Sakaue, H; Tomioka, A; Kaji, H; Sasaki, M; Orba, Y; Sawa, H; Kuno, A.
  Combinatorial Approach with Mass Spectrometry and Lectin Microarray Dissected Site-Specific Glycostem and Glycoleaf Features of the Virion-Derived Spike Protein of Ancestral and γ Variant SARS-CoV-2 Strains.
  J PROTEOME RES. 2024 Mar 27. doi: 10.1021/acs.jproteome.3c00874
• Miyashita, S; Ogura, T; Matsuura, S; Fukuda, E.
  Particle Size Measurement and Detection of Bound Proteins of Non-Porous/Mesoporous Silica Microspheres by Single-Particle Inductively Coupled Plasma Mass Spectrometry.
  MOLECULES. 2024 Feb 6. 29(5):1086. doi: 10.3390/molecules29051086
• Phuyathip, W; Putthisen, S; Panawan O, Ma-In P; Teeravirote, K; Sintusen, P; Udomkitkosol, S; Detarya, M; Luang. S; Mahalapbutr, P; Sato, T; Kuno, A; Chuangchaiya, S; Silsirivanit, A.
  Role of Wisteria floribunda agglutinin binding glycans in carcinogenesis and metastasis of cholangiocarcinoma.
  HISTOCHEM CELL BIOL. 2024 Feb 23. doi: 10.1007/s00418-024-02270-4
• Matsuda, A; Boottanun, P; Koizumi, S; Nagai, M; Kuno, A.
  Differential Glycoform Analysis of MUC1 Derived from Biological Specimens Using an Antibody-Overlay Lectin Microarray.
  METHODS MOL BIOL. 2024;2763:223-236. doi: 10.1007/978-1-0716-3670-1_19
• Niibori-Nambu, A; Yamasaki, Y; Kobayashi, D; Angata, K; Kuno, A; Panawan, O; Silsilivanit, A; Narimatsu, H; Araki, N.
  Chondroitin sulfate modification of CSPG4 regulates the maintenance and differentiation of glioma initiating cells via integrin-associated signaling.
  J BIOL CHEM. 2024 Feb 1:105706. doi: 10.1016/j.jbc.2024.105706
• Shimazaki, H; Nakamura, K; Ono, A; Segawa, O; Sawakami, K; Koizuka, M; Hirayama, M; Hori, K; Tajima, H; Kuno, A.
  Auto-Lectin Dotcoding by Two Octopuses: Rapid Analysis of Fluorescence-Labeled Glycoproteins by an 8-channel Fully-Automatic Bead Array Scanner with a Rolling-Circle Detector.
  ANAL CHEM. 2023 Aug 3. doi: 10.1021/acs.analchem.3c01395
• Boottanun, P; Nagai-Okatani, C; Nagai, M; Ungkulpasvich, U; Yamane, S; Yamada, M; Kuno, A.
  An improved evanescent fluorescence scanner suitable for high-resolution glycome mapping of formalin-fixed paraffin-embedded tissue sections.
  ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2023 JUL 3. doi: 10.1007/s00216-023-04824-2
• Tanaka, S; Sakaue, H, Koiwai, T; Okuda, N; Okuyama, K; Horioka, Y; Hiramatsu, Y; Kawashima, M; Ishiguro, N; Sato, T.
  Near-infrared radiation causes sebaceous gland enlargement along with an ROS-dependent augmentation of epidermal growth factor receptor expression in hamsters.
  EXPERIMENTAL DERMATOLOGY. 2023 JUL 4. doi: 10.1111/exd.14878
• Fuseya, S; Izumi, H; Hamano, A; Murakami, Y; Suzuki, R; Koiwai, R; Hayashi, T; Kuno, A, Takahashi, S; Kudo, T.
  Reduction in disialyl-T antigen levels in mice deficient for both St6galnac3 and St6galnac4 results in blood filling of lymph nodes.
  SCI REP. 2023 Jun 29;13(1):10582. doi: 10.1038/s41598-023-37363-y
• Matsuda, KM; Kotani, H; Yamaguchi, K; Okumura, T; Fukuda, E; Kono, M; Hisamoto, T; Kawanabe, R; Norimatsu, Y; Kuzumi, A; Fukayama, M; Fukasawa, T; Ebata, S; Yoshizaki-Ogawa, A; Okamura, T; Shoda, H; Fujio, K; Goshima, N; Sato, S; Yoshizaki, A.
  Significance of anti-transcobalamin receptor antibodies in cutaneous arteritis revealed by proteome-wide autoantibody screening.
  J AUTOIMMUN. 2023 Jan 30;135:102995. doi: 10.1016/j.jaut.2023.102995
• Itakura, Y; Hasegawa, Y; Kikkawa, Y; Murakami, Y; Sugiura, K; Nagai-Okatani, C; Sasaki, N; Umemura, M; Takahashi, Y; Kimura, T; Kuno, A; Ishiwata, T; Toyoda, M.
  Spatiotemporal changes of tissue glycans depending on localization in cardiac aging.
  REGEN THER. 2023 Jan 7;22:68-78. doi: 10.1016/j.reth.2022.12.009
• Zou, X; Lu, J; Deng, Y; Liu, Q; Yan, X; Cui, Y; Xiao, X; Fang, M; Yang, F; Sawaki, H; Sato, T; Tan, B; Lu, X; Feng, B; Kuno, A; Narimatsu, H; Gao, C; Zhang, Y.
  ST6GAL1 inhibits metastasis of hepatocellular carcinoma via modulating sialylation of MCAM on cell surface.
  ONCOGENE. 2022 Dec 17. doi: 10.1038/s41388-022-02571-9
• Tasaki, M; Tateno, H; Sato, T; Narimatsu, H; Saito, K; Nakagawa, Y; Aoki, T; Kamimura, M; Ushiki, T; Takahashi, K; Tomita, Y.
  Hyporesponsiveness against donors ABO antigens of renal grafts after ABO-incompatible kidney transplantation.
  CLIN EXP NEPHROL. 2022 Oct 8. doi: 10.1007/s10157-022-02280-3
• Hiono, T; Kuno, A.
  Glycan Profiling of Viral Glycoproteins with the Lectin Microarray.
  METHODS MOL BIOL. 2022;2556:59-68. doi: 10.1007/978-1-0716-2635-1_6
• Matsuda, KM; Yoshizaki, A; Yamaguchi, K; Fukuda, E; Okumura, T; Ogawa, K; Ono, C; Norimatsu, Y; Kotani, H; Hisamoto, T; Kawanabe, R; Kuzumi, A; Fukasawa, T; Ebata, S; Miyagawa, T; Yoshizaki-Ogawa, A; Goshima, N; Sato, S.
  Autoantibody Landscape Revealed by Wet Protein Array: Sum of Autoantibody Levels Reflects Disease Status.
  FRONT IMMUNOL. 2022 May 4;13:893086. doi: 10.3389/fimmu.2022.893086
• Kuroda, T; Hattori, Y; Kagami, A; Fukuda, E; Tamura, G.
  Genetic modification in the body to make friends with cancer cells?! Dreaming of cancer treatment in 100 years.
  CANCER SCIENCE. 113 1517-1517; FEB
• Fujimoto, M; Arai, E; Nishiyama, N; Maeshima, A; Fujimoto, H; Kitamura, H; Kuno, A; Yoshida, T; Kanai, Y.
  Lectin staining analysis of protein glycosylation in urothelial carcinomas.
  CANCER SCIENCE. 113 378-378; FEB
• Kitazume, Y; Arai, E; Matsuda, A; Kakuda, S; Ohara, K; Maeshima, A; Kuno, A; Yoshida, T; Kanai, Y.
  Alteration of protein glycosylation in renal carcinogenesis.
  CANCER SCIENCE. 113 1284-1284; FEB
• Nagata, Y; Kageyama, S; Ishikawa, T; Kokura, S; Okayama, T; Abe, T; Murakami, M; Otsuka, K; Ariyoshi, T; Kojima, T; Taniguchi, K; Kobayashi, S; Shimada, H; Yajima, S; Suzuki, T; Hirano, S; Tsuchikawa, T; Shichinohe, T; Ueda, S; Kanetaka, K; Yoneda, A; Wada, H; Doki, Y; Yamaue, H; Katsuda, M; Ohi, M; Yasuda, H; Kondo, K; Kataoka, M; Kodera, Y; Koike, M; Shiraishi, T; Miyahara, Y; Goshima,N;Fukuda, E; Yamaguchi, K; Sato, E; Ikeda, H; Yamada, T; Osako, M; Hirai, K; Miyamoto, H; Watanabe, T; Shiku, H.
  Prognostic significance of NY-ESO-1 antigen and PIGR expression in esophageal tumors of CHP-NY-ESO-1-vaccinated patients as adjuvant therapy.
  CANCER IMMUNOL IMMUNOTHER. 2022 Apr 16. doi: 10.1007/s00262-022-03194-5
• Tasaki, M; Tateno, H; Sato, T; Tomioka, A; Kaji, H; Narimatsu, H; Saito, K; Nakagawa, Y; Aoki, T; Kamimura, M; Ushiki, T; Okada, M; Miwa, Y; Hotta, K; Yoshida, Y; Takahashi, K; Tomita, Y.
  A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation.
  TRANSPL INT. 2022 Mar 23;35:10248. doi: 10.3389/ti.2022.10248
• Suzuki, R; Nakamura, Y; Koiwai, R; Fuseya, S; Murakami, Y; Hagiwara, K; Sato, T; Takahashi, S; Kudo, T.
  Global Loss of Core 1-Derived O-Glycans in Mice Leads to High Mortality Due to Acute Kidney Failure and Gastric Ulcers.
  INT J MOL SCI. 2022 Jan 24;23(3):1273. doi: 10.3390/ijms23031273
• Sogabe, M; Kojima, S; Kaya, T; Tomioka, A; Kaji, H; Sato, T; Chiba, Y; Shimizu, A; Tanaka, N; Suzuki, N; Hayashi, I; Mikami, M; Togayachi, A; Narimatsu, H.
  Sensitive New Assay System for Serum Wisteria floribunda Agglutinin-Reactive Ceruloplasmin That Distinguishes Ovarian Clear Cell Carcinoma from Endometrioma.
  ANALYTICAL CHEMISTRY; doi:10.1021/acs.analchem.1c04302
• Nagai-Okatani, C; Zou, X; Matsuda, A; Itakura, Y; Toyoda, M; Zhang, Y; Kuno, A.
  Tissue Glycome Mapping: Lectin Microarray-Based Differential Glycomic Analysis of Formalin-Fixed Paraffin-Embedded Tissue Sections.
  METHODS MOL BIOL. 2022;2460:161-180. doi: 10.1007/978-1-0716-2148-6_10
• Angata, K; Wagatsuma, T; Togayachi, A; Sato, T; Sogabe, M; Tajiri, K; Ozawa, T; Nagashima, I; Shimizu, H; Iijima, S; Korenaga, M; Kuno, A; Kaji, H; Mizokami, M; Narimatsu, H.
  O-glycosylated HBsAg peptide can induce specific antibody neutralizing HBV infection.
  BIOCHIM BIOPHYS ACTA GEN SUBJ. 2021 Sep 25:130020. doi: 10.1016/j.bbagen.2021.130020
• Noro, E; Matsuda,A; Kyoutou,T; Sato, T; Tomioka,A; Nagai,M; Sogabe,M; Tsuruno,C; Takahama,Y; Kuno, A; Tanaka,Y; Kaji, H; Narimatsu, H.
  N-glycan structures of Wisteria floribunda agglutinin-positive Mac2 binding protein in the serum of patients with liver fibrosis.
  GLYCOBIOLOGY. 2021 Jun 29:cwab060. doi: 10.1093/glycob/cwab060
• Sakata-Matsuzawa, M; Denda-Nagai, K; Fujihira, H; Noji, M; Ishii-Schrade, KB; Matsuda, A; Kuno, A; Okazaki, M; Nakai, K; Horimoto, Y; Saito, M; Irimura, T
  Glycans unique to the relapse-prone subset within triple-negative breast cancer as revealed by lectin array-based analysis of surgical specimens
  PLoS One. 2021 May 11;16(5):e0250747. doi: 10.1371/journal.pone.0250747. eCollection
• Nagai-Okatani C, Zou X, Fujita N, Sogabe I, Arakawa K, Nagai M, Angata K, Zhang Y, Aoki-Kinoshita KF, Kuno A
  LM-GlycomeAtlas Ver. 2.0: An Integrated Visualization for Lectin Microarray-based Mouse Tissue Glycome Mapping Data with Lectin Histochemistry.
  J PROTEOME RES. 2021 Mar 4. doi: 10.1021/acs.jproteome.0c00907
• Fujimoto M, Arai E, Matsuda A, Maeshima A, Fujimoto H, Kuno A, Yoshida T, Kanai Y
  Alterations of protein glycosylation and DNA methylation in urothelial carcinoma.
  CANCER SCIENCE 112 670-670; FEB
• Yokose T, Kabe Y, Matsuda A, Kitago M, Matsuda S, Hirai M, Nakagawa T, Masugi Y, Hishiki T, Nakamura Y, Shinoda M, Yagi H, Abe Y, Oshima G, Hori S, Nakano Y, Honda K, Kashiro A, Morizane C, Nara S, Kikuchi S, Shibahara T, Itonaga M, Ono M, Minegishi N, Koshiba S, Yamamoto M, Kuno A, Handa H, Sakamoto M, Suematsu M, Kitagawa Y
  O-Glycan-Altered Extracellular Vesicles: A Specific Serum Marker Elevated in Pancreatic Cancer
  Cancers (Basel) 12(9):E2469. (2020) doi: 10.3390/cancers12092469
• Angata K, Sawaki H, Tsujikawa S, Ocho M, Togayachi A, Narimatsu H
  Glycogene Expression Profiling of Hepatic Cells by RNA-Seq Analysis for Glyco-Biomarker Identification.
  FRONTIERS IN ONCOLOGY 10 57-77; (2020) doi: 10.3389/fonc.2020.01224
• Ito K, Angata K, Kuno A, Okumura A, Sakamoto K, Inoue R, Morita N, Watashi K, Wakita T, Tanaka Y, Sugiyama M, Mizokami M, Yoneda M, Narimatsu H
  Screening siRNAs against host glycosylation pathways to develop novel antiviral agents against hepatitis B virus
  HEPATOLOGY RESEARCH ; (2020) doi: 10.1111/hepr.13552
• Hiono T, Kuno A
  C-Terminally tagged NA in replication-competent influenza A viruses reveals differences in glycan profiles between NA and HA
  Analyst. 145(17): 5845-5853. (2020) doi: 10.1039/d0an00770f
• Angata K, Sawaki H, Tsujikawa S, Ocho M, Togayachi A, Narimatsu H.
  Glycogene expression profiling of hepatic cells by RNA-Seq analysis for glyco-biomarker identification.
  Frontiers In Oncology. 10:1224 (2020). doi: 10.3389/fonc.2020.01224
• Hiono T and Kuno A.
  C-terminally tagged NA in replication competent influenza A viruses reveals differences in glycan profiles between NA and HA.
  Analyst. Accepted(2020). doi:10.1039/D0AN00770F
• Silsirivanit A Matsuda A, Kuno A, Tsuruno C, Uenoyama Y, Seubwai W, Angata K, Teeravirote K, Wongkham C, Araki N, Takahama Y, Wongkham S, Narimatsu H.
  Multi-serum glycobiomarkers improves the diagnosis and prognostic prediction of cholangiocarcinoma.
  Clin Chim Acta. 510:142-149 (2020). doi: 10.1016/j.cca.2020.07.017
• Yamada I, Shiota M, Shinmachi D, Ono T, Tsuchiya S, Hosoda M, Fujita A, Aoki NP, Watanabe Y, Fujita N, Angata K, Kaji H, Narimatsu H, Okuda S, Aoki-Kinoshita KF.
  The GlyCosmos Portal: a unified and comprehensive web resource for the glycosciences.
  Nat Methods.17: 649-650 (2020). doi: 10.1038/s41592-020-0879-8
• Matsuda A, Kuno A^#*, Yoshida M, Wagatsuma T, Sato T, Miyagishi M, Suematsu M, Kabe Y, Narimatsu H
  Comparative glycomic analysis of exosome subpopulations derived from pancreatic cancer cell lines.
  J Proteom Res 19: 2516-2524 (2020). doi.org:10.1021/acs.jproteome.0c00200
• Ulaankhuu A, Bazarragchaa E, Okamatsu M, Hiono T, Bodisaikhan K, Amartuvshin T, Tserenjav J, Urangoo T, Buyantogtokh K, Matsuno K, Hattori T, Kondoh T, Sato M, Takadate Y, Torii S, Isono M, Okuya K, Saito T, Kasajima N, Kida Y, Maruyama J, Igarashi M, Takada A, Kida H, Batchuluun D, Sakoda Y.
  Genetic and Antigenic Characterization of H5 and H7 Avian Influenza Viruses Isolated From Migratory Waterfowl in Mongolia From 2017 to 2019
  Virus Genes. 19: 1-8 (2020). doi: 10.1007/s11262-020-01764-2
• Xu Z, Ku X, Tomioka A, Xie W, Liang T, Zou X, Cui Y, Sato T, Kaji H, Narimatsu H, Yan W, Zhang Y
  O-linked N-acetylgalactosamine modification is present on the tumor suppressor p53
  Biochim Biophys Acta Gen Subj. 1864: 129635 (2020) doi: 10.1016/j.bbagen.2020.129635
• Fuseya S, Suzuki R, Okada R, Hagiwara K, Sato T, Narimatsu H, Yokoi H, Kasahara M, Usui T, Morito N, Yamagata K, Kudo T, Takahashi S.
  Mice lacking core 1-derived O-glycan in podocytes develop transient proteinuria, resulting in focal segmental glomerulosclerosis.
  Biochem Biophys Res Commun. 523:1007-1013 (2020) doi: 10.1016/j.bbrc.2020.01.033
• Kikutani Y, Masatoshi, Shoko, Takase-Yoden S, Hiono T, de Vries RP, Ryan, Keita, Hiroshi, Yoshihiro
  E190V substitution of H6 hemagglutinin is one of key factors for binding to sulfated sialylated glycan receptor and infection to chickens
  Microbiol. Immunol. 73: 171-174 (2020) doi: 10.1111/1348-0421.12773
• Otani K, Tokudome T, Kamiya CA, Mao Y, Nishimura H, Hasegawa T, Arai Y, Kaneko M, Shioi G, Ishida J, Fukamizu A, Osaki T, Nagai-Okatani C, Minamino N, Ensho T, Hino J, Murata S, Takegami M, Nishimura K, Kishimoto I, Miyazato M, Harada-Shiba M, Yoshimatsu J, Nakao K, Ikeda T, Kangawa K.
  Deficiency of Cardiac Natriuretic Peptide Signaling Promotes Peripartum Cardiomyopathy-Like Remodeling in the Mouse Heart
  Circulation. 141: 571-588 (2020). doi: 10.1161/CIRCULATIONAHA.119.039761
• Shimazaki H, Ono A, Tsuruga M, Ueki A, Koseki-Kuno S, Toyoda T, Saito K, Sawakami K, Kariya M, Segawa O, Nakamura K, Koizuka M, Kuno A^*
  GlycoBIST: A System for Automatic Glycan Profiling of a Target Protein Using Milli-Bead Array in a Tip
  Curr Protoc Protein Sci. 99: e103 (2020) doi: 10.1002/cpps.103
• Wagatsuma T, Nagai-Okatani C^#, Matsuda A, Masugi Y, Imaoka M, Yamazaki K, Sakamoto M, Kuno A^*
  Discovery of Pancreatic Ductal Adenocarcinoma-Related Aberrant Glycosylations: A Multilateral Approach of Lectin Microarray-Based Tissue Glycomic Profiling With Public Transcriptomic Datasets
  Frontiers In Oncology 10: 338 (2020) doi: 10.3389/fonc.2020.00338
• Zou X, Yao F, Yang F, Zhang F, Xu ZJ, Shi JJ, Kuno A, Zhao H, Zhang Y
  Glycomic Signatures of Plasma IgG Improve Preoperative Prediction of the Invasiveness of Small Lung Nodules
  Molecules 25: 28 (2020) doi: 10.3390/molecules25010028
• Holdener BC, Percival CJ, Grady RC, Cameron DC, Berardinelli SJ, Zhang A, Neupane S, Takeuchi M, Jimenez-Vega JC, Uddin SMZ, Komatsu DE, Honkanen R, Dubail J, Apte SS, Sato T, Narimatsu H, McClain SA, Haltiwanger RS.
  ADAMTS9 and ADAMTS20 are differentially affected by loss of B3GLCT in mouse model of Peters plus syndrome.
  Hum Mol Genet. 28: 4053-4066 (2019) doi: 10.1093/hmg/ddz225
• Kurata I, Matsumoto I, Ohyama A, Osada A, Ebe H, Kawaguchi H, Kaneko S, Kondo Y, Tsuboi H, Tomioka A, Kaji H, Sumida T
  Potential involvement of OX40 in the regulation of autoantibody sialylation in arthritis
  Ann Rheum Dis. 78: 1488-1496 (2019) doi: 10.1136/annrheumdis-2019-215195
• Nagai-Okatani C^#*, Nishigori M, Sato T, Minamino N, Kaji H, Kuno A^*
  Wisteria floribunda agglutinin staining for the quantitative assessment of cardiac fibrogenic activity in a mouse model of dilated cardiomyopathy
  Lab Invest. 99: 1749-1765 (2019) doi: 10.1038/s41374-019-0279-9
• Shimazaki H, Saito K, Matsuda A, Sawakami K, Kariya M, Segawa O, , Miyashita Y, Ueda T, Koizuka M, Nakamura K, Kaji H, Tajima H, , Kuno A^*
  Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling
  Anal Chem. 91: 11162-11169 (2019) doi: 10.1021/acs.analchem.9b01876
• Saku A, Hirose K, Ito T, Iwata A, Sato T, Kaji H, Tamachi T, , Suto A, Goto Y, Domino SE, Narimatsu H, Kiyono H, Nakajima H
  Fucosyltransferase 2 induces lung epithelial fucosylation and exacerbates house dust mite-induced airway inflammation
  J. Allergy Clin Immunol. 144: 698-709 (2019) doi: 10.1016/j.jaci.2019.05.010
• Takahama H, Nishikimi T, Takashio S, Hayashi T, Nagai-Okatani C, Asada T, Fujiwara A, Nakagawa Y, Amano M, Hamatani Y, Okada A, Amaki M, Hasegawa T, Kanzaki H, Nishimura K, Yasuda S, Kangawa K, Anzai T, Minamino N, Izumi C.
  Change in the NT-proBNP/Mature BNP Molar Ratio Precedes Worsening Renal Function in Patients With Acute Heart Failure: A Novel Predictor Candidate for Cardiorenal Syndrome
  J Am Heart Assoc. 8: e011468 (2019). doi: 10.1161/JAHA.118.011468
• Nagai-Okatani C^#, Aoki-Kinoshita KF, Kakuda S, Nagai M, Hagiwara K, Kiyohara K, Fujita N, Suzuki Y, Sato T, Angata K, Kuno A^*
  LM-GlycomeAtlas Ver. 1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections
  Molecules. 24: 2962 (2019) doi: 10.3390/molecules24162962
• Takashio S, Takahama H, Nishikimi T, Hayashi T, Nagai-Okatani C, Matsuo A, Nakagawa Y, Amano M, Hamatani Y, Okada A, Amaki M, Hasegawa T, Kanzaki H, Yasuda S, Kangawa K, Anzai T, Minamino N, Izumi C.
  Superiority of Proatrial Natriuretic Peptide in the Prognostic Power in Patients With Acute Decompensated Heart Failure on Hospital Admission: Comparison With B-type Natriuretic Peptide and Other Natriuretic Peptide Forms
  Open Geart. 6: e001072 (2019). doi: 10.1136/openhrt-2019-001072
• Nakane T, Angata K, Sato T, Kaji H, Narimatsu H
  Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2
  J Biol Chem. 294: 7433-7444 (2019) doi: 10.1074/jbc.RA118.006892
• Shan A, Lu J, Xu Z, Li X, Xu Y, Li W, Liu F, Yang F, Sato T, Narimatsu H, Zhang Y.
  Polypeptide N-acetylgalactosaminyltransferase 18 non-catalytically regulates the ER homeostasis and O-glycosylation.
  Biochim Biophys Acta Gen Subj. 1863: 870-882 (2019). doi: 10.1016/j.bbagen.2019.01.009
• Nagai-Okatani C^#, Nagai M, Sato T, Kuno A^*
  An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
  Int J Mol Sci. 20: 700 (2019) doi: 10.3390/ijms20030700
• Shirabe K, Bekki Y, Gantumur D, Araki K, Ishii N, Kuno A, Narimatsu H, Mizokami M
  Response to the letter by Dr. Naoya Yamada, and Dr. Koichi Mizuta regarding our manuscript
  J Gastroenterol. 54: 206-207 (2019) doi: 10.1007/s00535-018-1524-5
• Matsumoto S, Ohara A, Nagai-Okatani C, Zhou YJ, Fujinaga D, Seike H, Nagata S
  Antagonistic Effect of Short Neuropeptide F on Allatotropin-Inhibited Feeding Motivation of the Silkworm Larva, Bombyx mori
  Zoolog Sci. 36: 58-67 (2019) doi: 10.2108/zs180119
• Hiono T^#*, Matsuda A, Wagatsuma T, Okamatsu M, Sakoda Y, Kuno A^*
  Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses
  Virology. 38: 103 (2019) doi: 10.1016/j.virol.2018.11.010
• Matsuo A, Nagai-Okatani C, Nishigori M, Kangawa K, Minamino N
  Natriuretic peptides in human heart: Novel insight into their molecular forms, functions, and diagnostic use
  Peptides. 11: 453-462 (2019) doi: 10.1016/j.peptides.2018.08.006
• Torii S, Matsuno K, Qiu Y, Mori-Kajihara A, Kajihara M, Nakao R, Nao N, Okazaki K, Sashika M, Hiono T, Okamatsu M, Sakoda Y, Ebihara H, Takada A, Sawa H.
  Infection of newly identified phleboviruses in ticks and wild animals in Hokkaido, Japan indicating tick-borne life cycles.
  Ticks Tick Borne Dis. 10: 328-335 (2019). doi: 10.1016/j.ttbdis.2018.11.012
• Solovieva E, Shikanai T, Fujita N, Narimatsu H
  GGDonto ontology as a knowledge-base for genetic diseases and disorders of glycan metabolism and their causative genes.
  J Biomed Semantics.9: 14 (2018). doi:10.1186/s13326-018-0182-0
• Narimatsu H, Kaji H, Vakhrushev SY, Clausen H, Zhang H, Noro E, Togayachi A, Nagai-Okatani C, Kuno A, Zou X, Cheng L, Tao SC, Sun Y
  Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics
  J Proteome Res. 17: 4097-4112 (2018) doi: 10.1021/acs.jproteome.8b00515
• Nagai-Okatani C^#, Nagata S, Nagasawa H
  Tissue distribution and biochemical characteristics of receptors for sinus gland peptide VII as a crustacean hyperglycemic hormone and vitellogenesis-inhibiting hormone of the kuruma prawn, Marsupenaeus japonicus
  Gen Comp Endocrinol. 266: 157-165 (2018) doi: 10.1016/j.ygcen.2018.05.010
• Wagatsuma T, Kuno A, Angata K, Tajiri K, Takahashi J, Korenaga M, Mizokami M, Narimatsu H
  Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment
  Anal Chem. 90: 10196-10203 (2018) doi: 10.1021/acs.analchem.8b01030
• Takahama H, Takashio S, Nishikimi T, Hayashi T, Nagai-Okatani C, Nakagawa Y, Amaki M, Ohara T, Hasegawa T, Sugano Y, Kanzaki H, Yasuda S, Kangawa K, Minamino N, Anzai T.
  Ratio of pro-B-type Natriuretic Peptide (BNP) to Total BNP Is Decreased in Mild, but Not Severe, Acute Decompensated Heart Failure Patients: A Novel Compensatory Mechanism for Acute Heart Failure.
  Int J Cardiol. 258: 165-171 (2018). doi: 10.1016/j.ijcard.2017.12.047
• Matsuura R, Kaji H, Tomioka A, Sato T, Narimatsu H, Moriwaki Y, Misawa H, Imai K, Tsuji S
  Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1
  Sci Rep. 8: 14251 (2018) doi: 10.1038/s41598-018-32534-8
• Shirabe K, Bekki Y, Gantumur D, Araki K, Ishii N, Kuno A, Narimatsu H, Mizokami M
  Mac-2 binding protein glycan isomer (M2BPGi) is a new serum biomarker for assessing liver fibrosis: more than a biomarker of liver fibrosis
  J Gastroenterol. 53: 819-826 (2018) doi: 10.1007/s00535-017-1425-z
• Wang ZJ, Kikutani Y, Nguyen LT, Hiono T, Matsuno K, Okamatsu M, Krauss S, Webby R, Lee YJ, Kida H, Sakoda Y.
  H13 influenza viruses in wild birds have undergone genetic and antigenic diversification in nature.
  Virus Genes. 54: 543-549 (2018). doi: 10.1007/s11262-018-1573-0
• Togayachi A, Tomioka A, Fujita M, Sukegawa M, Noro E, Takakura D, Miyazaki M, Shikanai T, Narimatsu H, Kaji H
  Identification of Poly-N-Acetyllactosamine-Carrying Glycoproteins from HL-60 Human Promyelocytic Leukemia Cells Using a Site-Specific Glycome Analysis Method, Glyco-RIDGE
  J Am Soc Mass Spectrom. 29: 1138-1152 (2018) doi: 10.1007/s13361-018-1938-6
• Narimatsu H, Sato T
  Wisteria floribunda agglutinin positive glycobiomarkers: a unique lectin as a serum biomarker probe in various diseases
  Expert Rev Proteomics. 15: 183-190 (2018) doi: 10.1080/14789450.2018.1419066
• Wakui H, Fuseya S, Suzuki R, Shimbo M, Okada R, Hamada M, Kuno A, , Hagiwara K, Sato T, Narimatsu H, Kudo T, Takahashi S
  Incomplete clearance of apoptotic cells by core 1-derived O-glycan-deficient resident peritoneal macrophages
  Biochem Biophys Res Commun. 495: 2017-2023 (2018) doi: 10.1016/j.bbrc.2017.12.066
• Suzuki R, Fujimoto Z, Kaneko S, Hasegawa T, and Kuno A^*.
  Enhanced azidolysis by the formation of stable Ser-His catalytic dyad in a glycoside hydrolase family 10 xylanase mutant.
  J Appl Glycosci 65: 1-8 (2018). doi.org/10.5458/jag.jag.JAG-2017_011
• Suzuki M, Okamatsu M, Hiono T, Matsuno K, Sakoda Y.
  Potency of an inactivated influenza vaccine prepared from A/duck/Hokkaido/162/2013 (H2N1) against a challenge with A/swine/Missouri/2124514/2006 (H2N3) in mice.
  J Vet Med Sci. 79: 1815-1821 (2017). doi: 10.1292/jvms.17-0312
• Nguyen LT, Nishi T, Shichinohe S, Chu DH, Hiono T, Matsuno K, Okamatsu M, Kida H, Sakoda Y.
  Selection of antigenic variants of an H5N1 highly pathogenic avian influenza virus in vaccinated chickens.
  Virology. 510: 252-261 (2017. doi: 10.1016/j.virol.2017.07.030
• Kanai Y, Yasoda A, Mori KP, Watanabe-Takano H, Nagai-Okatani C, , Yamashita Y, Hirota K, Ueda Y, Yamauchi I, Kondo E, Yamanaka S, , Sakane Y, Nakao K, Fujii T, Yokoi H, Minamino N, Mukoyama M, , Mochizuki N, Inagaki N
  Circulating osteocrin stimulates bone growth by limiting C-type natriuretic peptide clearance
  J. Clin Invest. 127: 4136-4147 (2017) doi: 10.1172/JCI94912
• Hiono T^#, Okamatsu M, Matsuno K, Haga A, Iwata R, Nguyen LT, Suzuki M, Kikutani Y, Kida H, Onuma M, Sakoda Y.
  Characterization of H5N6 highly pathogenic avian influenza viruses isolated from wild and captive birds in the winter season of 2016-2017 in Northern Japan.
  Microbiol Immunol. 61: 387-397 (2017). doi: 10.1111/1348-0421.12506
• Matsuda A, Higashi M, Nakagawa T, Yokoyama S, Kuno A^*, Yonezawa S, Narimatsu H
  Assessment of tumor characteristics based on glycoform analysis of membrane-tethered MUC1
  Lab Invest. 97: 1103-1113 (2017) doi: 10.1038/labinvest.2017.53
• Nguyen LT, Nakaishi K, Motojima K, Ohkawara A, Minato E, Maruyama J, Hiono T, Matsuno K, Okamatsu M, Kimura T, Takada A, Kida H, Sakoda Y.
  Rapid and broad detection of H5 hemagglutinin by an immunochromatographic kit using novel monoclonal antibody against highly pathogenic avian influenza virus belonging to the genetic clade 2.3.4.4.
  PLoS One. 12: e0182228 (2017). doi: 10.1371/journal.pone.0182228
• Togayachi A, Iwaki J, Kaji H, Matsuzaki H, Kuno A, Hirao Y, Nomura M, Noguchi M, Ikehara Y, Narimatsu H.
  Glycobiomarker, Fucosylated Short-Form Secretogranin III Levels Are Increased in Serum of Patients with Small Cell Lung Carcinoma.
  J Proteome Res. 16: 4495-4505 (2017). doi: 10.1021/acs.jproteome.7b00484
• Enkhbold B, Shatar M, Wakamori S, Tamura T, Hiono T, Matsuno K, Okamatsu M, Umemura T, Damdinjav B, Sakoda Y.
  Genetic and virulence characterization of classical swine fever viruses isolated in Mongolia from 2007 to 2015.
  Virus Genes. 53: 418-425 (2017). doi: 10.1007/s11262-017-1442-2
• Nagai-Okatani C^#, Kangawa K, Minamino N
  Three molecular forms of atrial natriuretic peptides: quantitative analysis and biological characterization
  J. Pept Sci. 121: 16964-16969 (2017) doi: 10.1002/psc.2969
• Okamatsu M, Ozawa M, Soda K, Takakuwa H, Haga A, Hiono T, Matsuu A, Uchida Y, Iwata R, Matsuno K, Kuwahara M, Yabuta T, Usui T, Ito H, Onuma M, Sakoda Y, Saito T, Otsuki K, Ito T, Kida H.
  Characterization of Highly Pathogenic Avian Influenza Virus A(H5N6), Japan, November 2016.
  Emerg Infect Dis. 23: 691-695 (2017). doi: 10.3201/eid2304.161957
• Nakatsukasa A, Kuruma K, Okamatsu M, Hiono T, Suzuki M, Matsuno K, Kida H, Oyamada T, Sakoda Y.
  Potency of whole virus particle and split virion vaccines using dissolving microneedle against challenges of H1N1 and H5N1 influenza viruses in mice.
  Vaccine. 35: 2855-2861 (2017). doi: 10.1016/j.vaccine.2017.04.009
• Ohkawara A, Okamatsu M, Ozawa M, Chu DH, Nguyen LT, Hiono T, Matsuno K, Kida H, Sakoda Y.
  Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia.
  Microbiol Immunol. 61: 149-158 (2017). doi: 10.1111/1348-0421.12478
• Bekki Y, Yoshizumi T, Shimoda S, Itoh S, Harimoto N, Ikegami T, Kuno A, Narimatsu H, Shirabe K, Maehara Y
  Hepatic stellate cells secreting WFA(+) -M2BP: Its role in biological interactions with Kupffer cells
  J Gastroenterol Hepatol. 32: 1387-1393 (2017) doi: 10.1111/jgh.13708
• Sato T^#, Tateno H, Kaji H, Chiba Y, Kubota T, Hirabayashi J, Narimatsu H
  Engineering of recombinant Wisteria floribunda agglutinin specifically binding to GalNAcbeta1,4GlcNAc (LacdiNAc)
  Glycobiology. 27: 743-754 (2017) doi: 10.1093/glycob/cwx038
• Mitani Y, Oshima Y, Mitsuda N, Tomioka A, Sukegawa M, Fujita M, Kaji H, Ohmiya Y
  Efficient production of glycosylated Cypridina luciferase using plant cells
  Protein Expr Purif. 133: 102-109 (2017) doi: 10.1016/j.pep.2017.03.008
• Shimbo M, Suzuki R, Fuseya S, Sato T, Kiyohara K, Hagiwara K, Okada R, Wakui H, Tsunakawa Y, Watanabe H, Kimata K, Narimatsu H, Kudo T, Takahashi S
  Postnatal lethality and chondrodysplasia in mice lacking both chondroitin sulfate N-acetylgalactosaminyltransferase-1 and -2
  PloS One. 12: e0190333 (2017) doi: 10.1371/journal.pone.0190333 56
• Tsuji S, Washimi K, Kageyama T, Yamashita M, Yoshihara M, Matsuura R, Yokose T, Kameda Y, Hayashi H, Morohoshi T, Tsuura Y, Yusa T, Sato T, Togayachi A, Narimatsu H, Nagasaki T, Nakamoto K, Moriwaki Y, Misawa H, Hiroshima K, Miyagi Y, Imai K.
  HEG1 Is a Novel Mucin-Like Membrane Protein That Serves as a Diagnostic and Therapeutic Target for Malignant Mesothelioma
  Sci Rep 7: 45768 (2017). doi: 10.1038/srep45768
• Zou X, Yoshida M, Nagai-Okatani C^#, Iwaki J, Matsuda A, Tan B, Hagiwara K, Sato T, Itakura Y, Noro E, Kaji H, Toyoda M, Zhang Y, Narimatsu H, Kuno A^*.
  A Standardized Method for Lectin Microarray-Based Tissue Glycome Mapping
  Sci Rep 7: 43560 (2017). doi: 10.1038/srep43560
• Tamaki N, Kuno A, Matsuda A, Tsujikawa H, Yamazaki K, Yasui Y, Tsuchiya K, Nakanishi H, Itakura J, Korenaga M, Mizokami M, Kurosaki M, Sakamoto M, Narimatsu H, Izumi N.
  Serum Wisteria Floribunda Agglutinin-Positive Sialylated Mucin 1 as a Marker of Progenitor/Biliary Features in Hepatocellular Carcinoma
  Sci Rep 7: 244 (2017). doi: 10.1038/s41598-017-00357-8
• Nakao K, Kuwahara K, Nishikimi T, Nakagawa Y, Kinoshita H, Minami T, Kuwabara Y, Yamada C, Yamada Y, Tokudome T, Nagai-Okatani C, Minamino N, Nakao YM, Yasuno S, Ueshima K, Sone M, Kimura T, Kangawa K, Nakao K.
  Endothelium-Derived C-Type Natriuretic Peptide Contributes to Blood Pressure Regulation by Maintaining Endothelial Integrity
  Hypertension. 69: 286-296 (2017) doi: 10.1161/HYPERTENSIONAHA.116.08219
• Shoda J, Matsuda A, Shida T, Yamamoto M, Nagino M, Tsuyuguchi T, Yasaka T, Tazuma S, Uchiyama K, Unno M, Ohkohchi N, Nakanuma Y, Kuno A, Narimatsu H.
  Wisteria Floribunda Agglutinin-Sialylated Mucin Core Polypeptide 1 Is a Sensitive Biomarker for Biliary Tract Carcinoma and Intrahepatic Cholangiocarcinoma: A Multicenter Study
  J Gastroenterol. 52: 218-228 (2017). doi: 10.1007/s00535-016-1230-0
• Zou X, Zhu MY, Yu DM, Li W, Zhang DH, Lu FJ, Gong QM, Liu F, Jiang JH, Zheng MH, Kuno A, Narimatsu H, Zhang Y, Zhang XX.
  Serum WFA + -M2BP Levels for Evaluation of Early Stages of Liver Fibrosis in Patients With Chronic Hepatitis B Virus Infection.
  Liver Int. 37: 35-44 (2017). doi: 10.1111/liv.13188