Cellular imaging Research Group

Research Outline

Molecular diagnostic technologies for realizing health visualization
To contribute to innovation in healthy lifestyles, we are developing novel technologies “for improving and protecting the health by using bioluminescence and fluorescence”. We are engineering various bioluminescence- and fluorescence-probes and novel optical measurement systems, and are investigating molecular and cellular mechanisms of biological systems. Based on findings in differences between various bioluminescence systems, our research group focuses on development of luminescent/fluorescent protein molecular probes, supports on commercial product developments in private companies, and novel technologies for “visualization of functions of circadian rhythm genes”, “in vivo imaging of cancer cells”, “development of drug screening devices”, and “establishment of cell-based toxicity testing for chemicals”.


  1. Kimura Y, Watanabe M, Suzuki N, Iwaki T, Yamakage K, Saito K, Nakajima Y, Fujimura C, Ohmiya Y, Omori T, Kojima H, Aiba S,
    The performance of an in vitro skin sensitisation test, IL-8 Luc assay (OECD442E), and the integrated approach with direct peptide reactive assay (DPRA)
    J. Toxicol. Sci., 43, 741-749 (2018) .
  2. Saiki P, Nakajima Y, Van Griensven Ljld,, Miyazaki K,
    Real-time monitoring of IL-6 and IL-10 reporter expression for anti-inflammation activity in live RAW 264.7cells.
    Biochem. Biophys. Res. Commun., 505, 885-890 (2018).
  3. Tabei Y, Sugino S, Nakajima Y, Horie M,
    Reactive oxygen species independent genotoxicity of indium tin oxide nanoparticles triggered by intracellular degradation.
    Food Chem. Toxicol., 118, 264-271 (2018).
  4. Hirano K, Iwaki T, Ishido T, Yoshikawa Y, Naruse K, Yoshikawa K,
    Stretching of single DNA molecules caused by accelerating flow on a microchip.
    J. Chem. Phys. 149, 165101 (2018).
  5. Uno K, Murotomi K, Kazuki Y, Oshimura M, Nakajima Y,
    Bioluminescence-based cytotoxicity assay for simultaneous evaluation of cell viability and membrane damage in human hepatoma HepG2 cells.
    Luminescence, 33, 616-624 (2018).
  6. Sato D, Abe S, Kobayashi K, Nakajima Y, Oshimura M, Kazuki Y,
    Human and mouse artificial chromosome technologies for studies of pharmacokinetics and toxicokinetics.
    Drug Metab. Pharmacokinet., 33, 17-30 (2018).
  7. Abe H, Ishioka M, Fujita Y, Umeno A, Yasunaga M, Sato A, Ohnishi S, Suzuki S, Ishida N, Shichiri M, Yoshida Y, Nakajima Y,
    Yuzu (Citrus junos Tanaka) peel attenuates dextran sulfate sodium-induced murine experimental colitis.
    J. Oleo Sci., 67, 335-344 (2018).
  8. Mie Y, Hirano Y, Kowata K, Nakamura A, Yasunaga M, Nakajima Y, Komatsu Y,
    Function control of anti-microRNA oligonucleotides using interstrand cross-linked duplexes.
    Mol. Ther. Nucleic Acids, 10, 64-74 (2018).
  1. Wakuri S, Yamakage K, Kazuki K, Kazuki M, Oshimura M, Abutani S, Yasunaga M, Nakajima Y,
    Correlation between luminescence intensity and cytotoxicity in cell-based cytotoxicity assay using luciferase.
    Anal. Biochem., 522, 18-29 (2017).
  2. Yasunaga M, Fujita Y, Saito R, Oshimura M, Nakajima Y,
    Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement.
    BMC Biotechnol., 17, 54 (2017).
  3. Ono D, Honma S, Nakajima Y, Kuroda, S, Enoki R, Honma K,
    Dissociation of Per1 and Bmal1 circadian rhythms in the suprachiasmatic nucleus in parallel with behavioral outputs.
    Proc. Natl. Acad. Sci. USA, 114, E3699-E3708 (2017).
  4. Tabei Y, Murotomi K, Umeno A, Horie M, Tsujino Y, Masutani B, Yoshida Y, Nakajima Y,
    Antioxidant properties of 5-hydroxy-4-phenyl-butenolide via activation of Nrf2/ARE signaling pathway.
    Food Chem. Toxicol., 107, 129-137 (2017).
  5. Tarnow P, Bross S, Wollenberg L, Nakajima Y, Ohmiya Y, Tralau T, Luch A,
    A novel dual-colour luciferase reporter assay for simultaneous detection of estrogen and aryl hydrocarbon receptor activation.
    Chem. Res. Toxicol., 30, 1436-1447 (2017).
  6. Futami M, Nakano T, Yasunaga M, Makihara M, Asama T, Hagihara Y, Nakajima Y, Futami J,
    Enhanced in-cell folding of reversibly cationized transcription factor using amphipathic peptide.
    J. Biosci. Bioeng., 123, 419-424 (2017).
  7. Tabei Y, Sugino S, Eguchi K, Tajika M, Abe H, Nakajima Y, Horie M,
    Effect of calcium carbonate particles shape on phagocytosis and pro-inflammatory response in differentiated THP-1 macrophages.
    Biochem. Biophys. Res. Commun., 490, 499-505 (2017).
  8. Horie M, Miura T, Hirakata S, Hosoyama A, Sugino S, Umeno A, Murotomi K, Yoshida Y, Koike T,
    Comparative analysis of the intestinal flora in type 2 diabetes and nondiabetic mice.
    Exp. Anim., 66, 405-416 (2017).
  1. Abe H, Tomimoton K, Fujita Y, Chiba Y, Nakayama K, and Nakajima Y,
    Development of N- and O-linked oligosaccharide engineered S. cerevisiae strain.
    Glycobiology, 26, 1248–1256 (2016).
  2. Murotomi K, Arai S, Uchida S, Endo S, Mitsuzumi H, Tabei Y, Yoshida Y, Nakajima Y, 
    Involvement of splenic iron accumulation in the development of nonalcoholic steatohepatitis in Tsumura Suzuki Obese Diabetes mice.
    Sci. Rep., 6, 22476 (2016).
  3. Tabei Y, Sonoda A, Nakajima Y, Biju V, Makita Y, Yoshida Y, Horie M,
    Intracellular accumulation of indium ions released from nanoparticles induces oxidative stress, proinflammatory response and DNA damage.
    J. Biochem., 159, 225-237 (2016).
  1. Yasunaga M, Murotomi K, Abe H, Yamazaki T, Nishii S, Ohbayashi T, Oshimura M, Noguchi T, Niwa K, Ohmiya Y, Nakajima Y,
    Highly sensitive luciferase reporter assay using a potent destabilization sequence of calpain 3.
    J. Biotechnol., 194, 115-23 (2015).
  2. Kimura Y, Fujimura C, Ito Y, Takahashi T, Nakajima Y, Ohmiya Y, Aiba S,
    Optimization of the IL-8 Luc assay as an in vitro test for skin sensitization.
    Toxicol. In Vitro, 29, 1816-1830 (2015).
  3. Yoshikawa T, Nakajima Y, Yamada Y, Enoki R, Watanabe K, Yamazaki M, Sakimura K, Honma S, Honma K,
    Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus.
    Eur. J. Neurosci., 42, 2678-2689 (2015).
  4. Murotomi K, Umeno A, Yasunaga M, Shichiri M, Ishida N, Abe H, Yoshida Y, Nakajima Y,
    Switching from singlet-oxygen-mediated oxidation to free-radical-mediated oxidation in the pathogenesis of type 2 diabetes in model mouse.
    Free Radic. Res., 49, 133-138 (2015).
  5. Murotomi K, Umeno A, Yasunaga M, Shichiri M, Ishida N, Koike T, Matsuo T, Abe H, Yoshida Y, Nakajima Y,
    Oleuropein-Rich Diet Attenuates Hyperglycemia and Impaired Glucose Tolerance in Type 2 Diabetes Model Mouse.
    J. Agri. Food Chem., 63, 6715-6722 (2015).
  6. Umeno A, Takashima M, Murotomi K, Nakajima Y, Koike T, Matsuo T, Yoshida Y,
    Radical-scavenging activity and antioxidative effects of olive leaf components oleuropein and hydroxytyrosol in comparison with homovanillic alcohol.
    J. Oleo Sci., 64, 793-800 (2015)
  7. Tabei Y, Sonoda A, Nakajima Y, Biju V, Makita Y, Yoshida Y, Horie M,
    In vitro evaluation of the cellular effect of indium tin oxide nanoparticles using the human lung adenocarcinoma A549 cells.
    Metallomics, 7, 816-827 (2015).
  8. Iwaki T, Ishido T, Hirano K, Lazutin A A, Vasilevskaya V V, Kenmotsu T, Yoshikawa K,
    Marked difference in conformational fluctuation between giant DNA molecules in circular and linear forms.
    J. Chem. Phys., 142, 145101 (2015).
  1. Yasunaga M, Nakajima Y, Ohmiya Y,
    Dual-color bioluminescence imaging assay using green- and red-emitting beetle luciferases at subcellular resolution.
    Anal. Bioanal. Chem., 406, 5735-42 (2014).
  2. Suzuki S, Murotomi K, Nakajima Y, Kawai K, Ohta K, Warita K, Miki T, Takeuchi Y,
    Development of an artificial calcium-dependent transcription factor to detect sustained intracellular calcium elevation.
    ACS Synth. Biol., 3, 717-722 (2014).
  3. Okabe T, Kumagai M, Nakajima Y, Shirotake S, Kodaira K, Oyama M, Ueno M, Ikeda M,
    The impact of HIF1α on the Per2 circadian rhythm in renal cancer cell lines.
    PLoS One,. 9, e109693 (2014).
  4. Murotomi K, Umeno A, Yasunaga M, Shichiri M, Ishida N, Abe H, Yoshida Y, Nakajima Y,
    Type 2 diabetes model TSOD mouse is exposed to oxidative stress at young age.
    J. Clin. Biochem. Nutr., 55, 216-220 (2014).
  1. Tomimoto K, Fujita Y, Iwaki T, Chiba Y, Jigami Y, Nakayama K, Nakajima Y, Abe H,
    Protease-deficient Saccharomyces cerevisiae strains for the synthesis of human-compatible glycoproteins.
    Biosci. Biotechnol. Biochem., 77, 2461-2466 (2013).
  2. Koinuma S, Asakawa T, Nagano M, Furukawa K, Sujino M, Masumoto KH, Nakajima Y, Hashimoto S, Yagita K, Shigeyoshi Y,
    Regional circadian period difference in the suprachiasmatic nucleus of the mammalian circadian center.
    Eur. J. Neurosci,. 38, 2832-2841 (2013).
  3. Yamada Y, Nishide SY, Nakajima Y, Watanabe T, Ohmiya Y, Honma K, Honma S,
    Monitoring circadian time in rat plasma using a secreted Cypridina luciferase reporter.
    Anal. Biochem., 439, 80-87 (2013).
  4. Yang F, Inoue I, Kumagai M, Takahashi S, Nakajima Y, Ikeda M,
    Real-time analysis of the circadian oscillation of the Rev-Erbβ promoter.
    J. Atheroscler. Thromb., 20, 267-276 (2013).
  5. Morigaki K, Mizutani K, Saito M, Okazaki T, Nakajima Y, Tatsu Y, Imanishi H,
    Surface functionalization of a polymeric lipid bilayer for coupling a model biological membrane with molecules, cells, and microstructures.
    Langmuir, 29, 2722-2730 (2013).
  6. Hirano K, Ishido T, Yamamoto Y S, Murase N, Baba Y, Itoh T,
    Plasmonic imaging of Brownian motion of single DNA molecules spontaneously binding to Ag nanoparticles.
    Nano Lett., 13, 1877-1882 (2013).
  7. Yamamoto Y S, Hirano K, Ishido T, Yasui T, Murase N, Baba Y, Itoh T,
    Plasmonic staining of DNA molecules with photo-induced Ag nanopraticles monitored using dark-field microscopy.
    Phys Chem. Chem. Phys., 15, 10316-10320 (2013).
  8. Frusawa H, Manabe T, Kagiyama E, Hirano K, Kameta N, Masuda M, Shimizu T,
    Electric moulding of dispersed lipid nanotubes into a nanofluidic device.
    Sci. Rep., 3, 2165 (2013).
  1. Noguchi T, Ikeda M, Ohmiya Y, Nakajima Y,
    A dual-color luciferase assay system reveals circadian resetting of cultured fibroblasts by co-cultured adrenal glands.
    PLoS One, 7, e37093 (2012).
  2. Myung J, Hong S, Hatanaka F, Nakajima Y, De Schutter E, Takumi T,
    Period coding of Bmal1 oscillators in the suprachiasmatic nucleus.
    J.Neurosci., 32, 8900-8918 (2012).
  3. Tahara Y, Kuroda H, Saito K, Nakajima Y, Kubo Y, Ohnishi N, Seo Y, Otsuka M, Fuse Y, Ohura Y, Komatsu T, Moriya Y, Okada S, Furutani N, Hirao A, Horikawa K, Kudo T, Shibata S,
    In Vivo monitoring of peripheral circadian clocks in the mouse.
    Curr. Biol., 22:1029-1034 (2012).
  4. Miyako E, Deguchi T, Nakajima Y, Yudasaka M, Hagihara Y, Horie M, Shichiri M, Higuchi Y, Yamashita F, Hashida M, Shigeri Y, Yoshida Y, Iijima S,
    Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns.
    Proc. Natl.Acad. Sci. USA, 109, 7523-7528 (2012).
  5. Hayashi K, Yano S, Abe H, Fujita Y, Kishida M,
    Improvement of the growth defect in salt- and ethanol-tolerant yeast mutagenized with error-prone DNA polymerization by using backcross cell fusion.
    J. Biosci. Bioeng., 114, 476-478 (2012).
  6. Syamala KM, Abe H, Fujita Y, Tomimoto K, Biju V, Ishikawa M, Ozaki Y, Itoh T,
    Inhibition assay of yeast cell walls by plasmon resonance Rayleigh scattering and surface-enhanced Raman scattering imaging.
    Langmuir. 28, 8592-8958 (2012).
  7. Hirano K, Ichikawa M, Ishido T, Ishikawa M, Baba Y, Yoshikawa K,
    How Environmental Solution Conditions Determine the Compaction Velocity of Single DNA Molecules.
    Nucleic Acids Res., 40, 284-289 (2012).
  8. Myobatake Y, Takeuchi T, Kuramochi K, Kuriyama I, Ishido T, Hirano K, Sugawara F, Yoshida H, Mizushina Y,
    Pinophilins A and B, inhibitors of mammalian A-, B- and Y-families of DNA polymerases and human cancer cell proliferation.
    J. Nat. Prod., 75, 135-141 (2012).
  9. Murotomi K, Takagi N, Muroyama A, Kaji N, Takeo S, Tanonaka K,
    Transient focal cerebral ischemia differentially decreases Homer1a and 1b/c contents in the postsynaptic density.
    Neurosci. Lett., 515, 92-96 (2012).
  1. Takahashi T, Kimura Y, Saito R, Nakajima Y, Ohmiya Y, Yamasaki K, Aiba S,
    An in vitro test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8.
    Toxicol. Sci. 124, 359-369 (2011).
  2. Yatsushiro S, Akamine R, Yamamura S, Hino M, Kajimoto K, Abe K, Abe H, Kido J, Tanaka M, Shinohara Y, Baba Y, Ooie T, Kataoka M,
    Quantitative analysis of serum procollagen type I C-terminal propeptide by immunoassay on microchip.
    PLoS One,13, e18807 (2011).
  3. Yamaguchi Y, Yatsushiro S, Yamamura S, Abe H, Abe K, Watanabe M, Kajimoto K, Shinohara Y, Baba Y, Kataoka M,
    Ribonuclease protection assay on microchip electrophoresis.
    Analyst, 136, 2247-2251 (2011).
  4. Ishido T, Yamazaki N, Ishikawa M, Hirano K,
    Characterization of DNA polymerase β from Danio rerio by overexpression in E. coli using the in vivo/in vitro compatible pIVEX plasmid.
    Microb. Cell Fact., 10, 84 (2011).
  5. Kitahama Y, Itoh T, Ishido T, Hirano K, Ishikawa M,
    Surface-enhanced Raman Scattering from Photo-reduced Ag Nanoaggregates on an Optically-trapped Single Bacterium.
    Bull. Chem. Soc. Jpn., 84, 976-978 (2011).
  6. Murotomi K, Takagi N, Takeo S, Tanonaka K,
    NADPH oxidase-mediated oxidative damage to proteins in the postsynaptic density after transient focal cerebral ischemia and reperfusion.
    Mol. Cell. Neurosci., 46, 681-688 (2011).
  1. Noguchi T, Michihata T, Nakamura W, Takumi T, Shimizu R, Yamamoto M, Ikeda M, Ohmiya Y, Nakajima Y,
    Dual-color luciferase mouse directly demonstrates coupled expression of two clock genes.
    Biochemistry, 49, 8053-8061 (2010).
  2. Nakajima Y, Yamazaki T, Nishii S, Noguchi T, Hoshino H, Niwa K, Viviani VR, Ohmiya Y,
    Enhanced beetle luciferase for highresolution bioluminescence imaging.
    PLoS One, 5, e10011 (2010).
  3. Lee Y, Lee J, Kwon I, Nakajima Y, Ohmiya Y, Son GH, Lee KH, Kim K,
    Coactivation of the CLOCK-BMAL1 complex by CBP mediates resetting of the circadian clock.
    J.Cell Sci., 123, 3547-3557 (2010).
  4. Niwa K, Ichino Y, Kumata S, Nakajima Y, Hiraishi Y, Kato D, Viviani VR, Ohmiya Y,
    Quantum yields and kinetics of the firefly bioluminescence reaction of beetle luciferase.
    Photochem. Photobiol., 86, 1046-1049 (2010).
  5. Kwon H, Enomoto T, Shimogawara M, Yasuda K, Nakajima Y, Ohmiya Y,
    Bioluminescence imaging of dual gene expression at single-cell level.
    BioTechniques, 10, 460-462 (2010).
  6. Niwa K, Nakajima Y, Ohmiya Y,
    Bioluminescence assays for L-cysteine and luciferase.
    Anal. Biochem., 396, 316-318 (2010).
  7. Li X, Nakajima Y, Niwa K, Viviani VR,. Ohymiya Y,
    Enhanced red-emitting railroad worm luciferase for bioassays and bioimaging.
    Protein Sci., 19, 26-33 (2010).
  8. Takahashi S, Inoue I, Nakajima Y, Seo M, Nakano T, Kumagai M, Yang F, Kodama T, Awata T, Ikeda M, Katayama S,
    A promoter in the novel exon of hPPARγ directs the circadian expression of PPARγ.
    J. Atheroscler. Thromb., 17, 73-83 (2010).
  9. Hirano K, Yoshida Y, Ishido T, Wada Y, Moriya N, Yamazaki N, Mizushina Y, Baba Y, Ishikawa M,
    Consecutive incorporation of fluorochrome-labeled nucleotides by mammalian DNA polymerase beta.
    Anal. Biochem., 405, 160-167 (2010).
  10. Ishido T, Ishikawa M, Hirano K,
    Analysis of supercoiled DNA by agarose gel electrophoresis using low-conducting sodium threonine medium.
    Anal. Biochem., 400, 148-150 (2010).
  11. Kamiya K, Hamabe W, Tokuyama S, Hirano K, Satake T, Kumamoto-Yonezawa Y, Yoshida H, Mizushina Y,
    Inhibitory effect of anthraquinones isolated from the Noni (Morinda citrifolia) root on animal A, B and Y families of DNA polymerases and human cancer cell proliferation.
    Food Chem., 118, 725-730 (2010).
  12. Murotomi K, Takagi N, Mizutani R, Honda TA, Ono M, Takeo S, Tanonaka K,
    mGluR1 antagonist decreases NADPH oxidase activity and superoxide production after transient focal cerebral ischemia.
    J. Neurochem., 114, 1711-1719 (2010).
  1. Yang F, Nakajima Y, Kumagai M, Ohmiya Y, Ikeda M,
    The molecular mechanism regulating the autonomous circadian expression of Topoisomerase I in NIH3T3 cells.
    Biochem. Biophys. Res. Commun., 380, 22-27 (2009).
  2. Abe H, Fujita Y, Takaoka Y, Kurita E, Yano S, Tanaka N, Nakayama K,
    Ethanol-tolerant Saccharomyces cerevisiae strains isolated under selective conditions by over-expression of a proofreading-deficient DNA polymerase delta.
    J. Biosci. Bioeng.,108, 199-204 (2009).
  3. Abe H, Fujita Y, Chiba Y, Jigami Y, Nakayama K,
    Upregulation of genes involved in gluconeogenesis and the glyoxylate cycle suppressed the drug sensitivity of an N-glycan-deficient Saccharomyces cerevisiae mutant.
    Biosci. Biotechnol. Biochem., 73, 1398-1403 (2009).
  4. Abe H, Takaoka Y, Chiba Y, Sato N, Ohgiya S, Itadani A, Hirashima M, Shimoda C, Jigami Y, Nakayama K,
    Development of valuable yeast strains using a novel mutagenesis technique for the effective production of therapeutic glycoproteins.
    Glycobiology, 19, 428-436 (2009).
  5. Hirano K, Ishido T, Ishikawa M,
    Rapid sequencing gel electrophoresis by using glycerol-tolerant sodium taurine medium.
    Anal. Biochem., 390, 100-101 (2009).
  6. Hirano K. Aoyagi M, Ishido T, Ooie T, Frusawa H, Asakawa M, Shimizu T, Ishikawa M,
    Measuring the length distribution of self-assembled lipid nanotubes by orientation control with a high frequency electric field in aqueous solutions.
    Anal. Chem., 81, 1459-1464 (2009).
  7. Mizushina Y, Motoshima H, Yamaguchi Y, Takeuchi T, Hirano K, Sugawara F, Yoshida H,
    3-O-Methylfunicone, a Selective Inhibitor of Mammalian Y-Family DNA Polymerases from a Fungal Strain from Australian Sea Salt.
    Mar. Drugs, 7, 624-639 (2009).
  8. Miyako E, Nagata H, Funahashi R, Hirano K, Hirotsu T,
    Light-Driven Thermoelectric Conversion Based on a Carbon Nanotube-Ionic Liquid Gel Composite.
    ChemSusChem, 2, 740-742 (2009).
  9. Miyako E, Nagata H, Hirano K, Hirotsu T,
    Micropatterned Carbon Nanotube-Gel Composite as Photothermal Material.
    Adv. Mater., 21, 2819-2823 (2009).
  10. Miyako E, Nagata H, Funahashi R, Hirano K, Hirotsu T,
    Light-Triggered Thermoelectric Conversion Based on a Carbon Nanotube-Polymer Hybrid Gel.
    ChemSusChem, 2, 419-422 (2009).
  11. Miyako E, Nagata H, Hirano K, Hirotsu T,
    Laser-triggered Carbon Nanotube Microdevice for Remote Control.
    Lab Chip, 9, 788-794 (2009).
  12. Takenaga Y, Takagi N, Murotomi K, Tanonaka K, Takeo S,
    Inhibition of Src activity decreases tyrosine phosphorylation of occludin in brain capillaries and attenuates increase in permeability of the blood–brain barrier after transient focal cerebral ischemia.
    J. Cereb. Blood Flow Metab., 29, 1099-1108 (2009).
  1. Noguchi T, Ikeda M, Ohmiya Y, Nakajima Y,
    Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases.
    BMC Biotechnol., 8, 40 (2008).
  2. Miyamoto N, Izumi H, Noguchi T, Nakajima Y, Ohmiya Y, Shiota M, Kidani A, Tawara A, Kohno K,
    Tip60 is regulated by circadian transcription factor clock and is involved in cisplatin resistance.
    J. Biol. Chem., 283, 18218-18226 (2008).
  3. Sujith A, Itoh T, Abe H, Yoshida K, Anas AA, Biju VP, Ishikawa M,
    Surface enhanced Raman scattering analyses of individual silver nanoaggregates on living single yeast cell wall.
    Appl. Phys. Lett., 92:103901-103903 (2008).
  4. Hirano K, Nagata H, Ishido T, Tanaka Y, Baba Y, Ishikawa M,
    Sizing of single globular DNA molecules by using a circular acceleration technique with laser trapping.
    Anal. Chem., 80, 5197-5202 (2008).
  5. Miyako E, Nagata H, Hirano K, Hirotsu T,
    Photodynamic thermoresponsive nanocarbon-polymer gel hybrids.
    Small, 4, 1711-1715 (2008).
  6. Miyako E, Nagata H, Hirano K, Makita Y, Nakayama K, Hirotsu T,
    Photoinduced antiviral carbon nanohorns.
    Nanotechnology, 19, 075106-075111 (2008).
  7. Miyako E, Nagata H, Hirano K, Makita Y, Hirotsu T,
    Photodynamic release of fullerenes from within carbon nanohorn.
    Chem. Phys. Lett., 456, 220-222 (2008).
  8. Miyako E, Nagata H, Hirano K, Hirotsu T,
    Carbon nanotube-polymer composite for light-driven microthermal control.
    Angew. Chem. Int. Ed., 47, 3610-3613 (2008).
  9. Nagata H, Ishikawa M, Yoshida Y, Tanaka Y, Hirano K,
    Use of a heterogeneous buffer combination in microchip electrophoresis for high-resolution separation by on-line concentration of DNA samples.
    Electrophoresis, 29, 3744-3751 (2008).
  10. Tanaka Y, Kawada H, Hirano K, Ishikawa M, Kitajima H,
    Automated manipulation of non-spherical micro-objects using optical tweezers combined with image processing techniques.
    Opt. Exp., 16, 15117-15122 (2008).
  11. Murotomi K, Takagi N, Takayanagi G, Ono M, Takeo S, Tanonaka K,
    mGluR1 antagonist decreases tyrosine phosphorylation of NMDA receptor and attenuates infarct size after transient focal cerebral ischemia.
    J. Neurochem., 105, 1625-1634 (2008).
  12. Akita H, Takagi N, Ishihara N, Takagi K, Murotomi K, Funakoshi H, Matsumoto K, Nakamura T, Takeo S,
    Hepatocyte growth factor improves synaptic localization of the NMDA receptor and intracellular signaling after excitotoxic injury in cultured hippocampal neurons.
    Exp. Neurol., 210, 83-94 (2008).
  1. Hoshino H, Nakajima Y, Ohmiya Y,
    Single-cell imaging with fluorescent protein excited by luciferin-luciferase reaction.
    Nature Methods, 8, 637-639 (2007).
  2. Tsuboi Y, Abe H, Nakagawa R, Oomizu S, Watanabe K, Nishi N, Nakamura T, Yamauchi A and Hirashima M,
    Galectin-9 protects mice from the Swaartzman reaction by attracting prostaglandin E2-producing polymorphonuclear leukocytes.
    Clinical Immunol., 124:221-233 (2007).
  3. Miyanishi N, Nishi N, Abe H, Kashio Y, Shinonaga R, Nakakita S, Sumiyoshi W, Yamauchi A, Nakamura T, Hirashima M, Hirabayashi J,
    Carbohydrate-recognition domains of galectin-9 are involved in intermolecular interaction with galectin-9 itself and other members of the galectin family.
    Glycobiology, 17, 423-32 (2007).
  4. Miyako E, Nagata H, Hirano K, Makita Y, Nakayama K, Hirotsu T,
    Near-infrared laser-triggered carbon nanohorns for selective elimination of microbes.
    Nanotechnology, 18, 475103-475110 (2007).
  5. Tanaka Y, Hirano K, Nagata H, Ishikawa M,
    Real-time three-dimensional orientation control of non-spherical micro-objects using laser trapping.
    Electron. Lett., 43, 412-413 (2007).
  1. Nishide S, Honma S, Nakajima Y, Ikeda M, Baba K, Ohmiya Y, Honma K,
    New reporter system for Per1 and Bmal1 expressions revealed self-sustained circadian rhythms in peripheral tissues.
    Genes Cells, 11, 1173-1182 (2006).
  2. Maeda E, Hirano K, Baba Y, Nagata H, Tabuchi M,
    Conformational separation of monosaccharides of glycoproteins labeled with 2-aminoacrydone using microchip electrophoresis.
    Electrophoresis, 27, 2002-2010 (2006).
  1. Nakajima Y, Kimura T, Sugata K, Enomoto T, Asakawa A, Kubota H, Ikeda M , Ohmiya Y,
    A multicolor luciferase assay system, one-step monitoring of multiple gene expressions with a single substrate.
    BioTechniques, 38:891-894 (2005).
  2. Suzuki C, Nakajima Y, Akimoto H, Wu C, Ohmiya Y,
    A new additional reporter enzyme, dinoflagellate luciferase, for monitoring of gene expression in mammalian cells.
    Gene, 344, 61-66 (2005).
  3. Nagata H, Tabuchi M, Hirano K, Baba Y,
    Microchip electrophoretic protein separation using electroosmotic flow induced by dynamic SDS-coating of uncoated plastic chips.
    Electrophoresis, 26, 2247-2253 (2005).
  4. Nagata H, Tabuchi M, Hirano K, Nobumasa H, Higasa M, Murakami Y, Yamazaki Y, Baba Y,
    High speed separation of proteins by microchip electrophoresis using a PEG-coated plastic chip with an SDS-linear polyacrylamide solution.
    Electrophoresis, 26, 2687-2691 (2005).
  5. Nagata H, Tabuchi M, Hirano K, Baba Y,
    Automatic protein separation by microchip electrophoresis using quartz chip.
    Chromatography, 26, 23-28 (2005).
  1. Nakajima Y, Ikeda M, Kimura T, Honma S, Ohmiya Y, Honma K,
    Bidirectional role of orphan nuclear receptor RORα in clock gene transcriptions demonstrated by a novel reporter assay system.
    FEBS Lett., 565, 122-126 (2004).
  2. Nakajima Y, Kimura T, Suzuki C, Ohmiya Y,
    Improved expression of novel red- and green-emitting luciferases of Phrixothrix railroad worms in mammalian cells.
    Biosci. Biotechniol. Biochem., 68, 948-951 (2004).
  3. Nakajima Y, Kobayashi K, Yamagishi K, Enomoto T, Ohmiya Y,
    cDNA cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca.
    Biosci. Biotechnol. Biochem., 68, 565-570 (2004).
  4. Abe H, Ohba H, Shimma H and Jigami Y,
    Yeast cells harboring human α-1, 3 -fucosyltransferase at the cell surface engineered using Pir, a cell wall-anchored protein.
    FEMS Yeast Res., 4, 417-425 (2004).
  5. Nakamura T, Abe H, Shimoda C,
    ADAM family protein Mde10 is essential for development of spore envelopes in the fission yeast Schizosaccharomyces pombe.
    Eukaryotic Cell, 3, 27-39 (2004).

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