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AIST-INDIA 機能性資源連携研究室

研究概要

我々は、細胞培養、生化学・分子生物学、バイオインフォマティクスなどを統合した手法を用い、ストレス、加齢、がんなどの生命現象の理解及びそれらに介入する独自技術の開発に取り組んでいます。
既存技術では不可能だった細胞機能の解析を実現し、ヒト正常細胞で厳密に制御される細胞分裂と増殖が、がん化により破綻するメカニズムに注目し研究を進めています。
このプロジェクトのために、インド国内の様々な研究機関との国際連携を行っており、学生や若手研究者育成のための様々なプログラムを推進しています。
参照 http://web.iitd.ac.in/~sundar/dailab/ AIST-INDIA 機能性資源連携研究室

研究課題

研究課題1:がん標的としてのモータリンとCARFの検証:ストレス及びがん生物学におけるその役割の分子的理解、および介入ツールと技術の開発
研究担当者:ワダワ レヌー

我々が独自にクローニングした2つのタンパク質、モータリンとCARFは、がん細胞で過剰に発現することから、抗がん剤の新たな標的として注目されています。現在、それらのタンパク質について、以下のような検証を進めています。
(i)ストレス、老化及びがん生物学における役割の解明、
(ii)ストレス及びがん診断への有用性、
(iii)ストレス及びがん介入のための阻害剤としての利用

AIST-INDIA機能性資源連携研究室2

研究課題

研究課題2:分子バイオメカニクスに基づく生命現象の動的原理の解明
研究担当者:中村 史、秋葉 龍郎、金 賢徹、山岸 彩奈

直径200nmのナノニードルやお椀形状磁気微粒子マグカップなど独自のナノ材料技術を駆使して、生命の精密かつ柔軟な動作原理を分子レベルで理解する研究を推進します。具体的には、細胞に極めてダメージの小さいナノニードル技術を用いた生細胞内での分子計測と分子操作、転移に関与する新規がんマーカーの機械的機能の解析とこれを標的とした診断および治療技術の開発、センシング機能搭載マグカップを用いたマーカー分子微量発現がん細胞の高感度検出と解析などの研究を行っています。

AIST-INDIA機能性資源連携研究室3

研究課題

研究課題3:細胞を用いた新たな機能性評価系の開発とその応用
研究担当者:冨田 辰之介

天然資源等からの新規な機能性物質探索を行うために、バイオアッセイを中心に実験を進めています。バイオアッセイとしては、培養細胞を用いて、毒性評価、免疫応答性、生体リズムについて、生物発光を取り入れた新しい検出方法を開発しています。さらにこの開発した技術を用いて新たな生理活性物質を探索しています。植物などの二次代謝産物から生理活性物質を探索しますが、アッセイには粗精製物も適用可能です。その中から必要に応じて活性物質の単離や同定を行い、活性物質の作用点やメカニズムを明らかにする研究も行っています。

AIST-INDIA機能性資源連携研究室4

研究課題

研究課題4:圧力駆動型MPSの開発と産業応用
研究担当者:杉浦 慎治

微細加工技術を利用して生理学性の高い培養環境を創出し、高次の細胞・臓器機能を発現可能な生体模倣システム(Microphysiological systems、MPS)の開発と産業応用を目指します。

AIST-INDIA機能性資源連携研究室5

メンバー

名前 役職 主な研究テーマ
ワダワ レヌー WADHWA Renu 連携研究室長 ・がん発生におけるモータリンの分子機構解析
・アシュワガンダによるがんの抑制と治療
・ストレス改善の為のCARFメカニズム解析
杉浦 慎治 SUGIURA Shinji 副連携研究室長 ・Microphysiological systemsの開発と創薬への応用
・マイクロプロセを用いた微小培養環境制御
・光分解性ゲルを用いた細胞分離
中村 史 NAKAMURA Chikashi 連携研究室付 ・中間径フィラメント捕縛による転写制御機構の解明
・ナノニードルを用いた安全・高効率物質導入技術の開発
・悪性がん細胞の機械的特性に着目した浸潤性制御
冨田 辰之介 TOMITA Tatsunosuke 主任研究員 ・生体リズムを指標とした生理活性物質探索
・生理活性物質探索に適したレポータ細胞の設計構築
・細胞の培養方法を工夫した新たなアッセイ方法開発
山岸 彩奈 YAMAGISHI Ayana 研究員 ・がん細胞の転移に関わる中間径フィラメントの機能解明
・癌細胞の弾性率と転移性の関係解明
・癌転移性評価方法の開発
吉富 廉 YOSHITOMI Ren 研究員 ・新規生体模倣アッセイ系の開発
・機能性分子の探索ならびにそのメカニズム解明
・環状RNAの機能解析
カウル スニル KAUL Sunil 招聘研究員

業績リスト

  • Huifu, H; Shefrin, S; Yang, S; Zhang, Z; Kaul, SC; Sundar, D; Wadhwa, R.
    Cucurbitacin-B inhibits cancer cell migration by targeting mortalin and HDM2: computational and in vitro experimental evidence.
    J BIOMOL STRUCT DYN. 2023 May 2:1-10. doi: 10.1080/07391102.2023.2206914
  • Zhang, H; Wang, J; Prakash, J; Zhang, Z; Kaul, SC; Wadhwa, R.
    Three-way Cell-based Screening of Antistress Compounds: Identification, Validation, and Relevance to Old-age Related Pathologies.
    J GERONTOL A BIOL SCI MED SCI. 2023 Apr 15:glad103. doi: 10.1093/gerona/glad103
  • Sugiura, S; Yamahira, S; Tamura, M; Shin, K; Shibuta, M; Satoh, T; Matsuzawa, Y; Fujii, G; Yanagawa, F; Mutoh, M; Yanagisawa, M; Kato, R; Matsui, H.
    Automated cell isolation from photodegradable hydrogel based on fluorescence image analysis.
    BIOTECHNOL BIOENG. 2023 Mar 15. doi: 10.1002/bit.28375
  • Kumar, V; Sari, AN; Meidinna, HN; Kaul, A; Basu, B; Ishida, Y; Terao, K; Kaul, SC; Vrati, S; Sundar, D; Wadhwa, R.
    Computational and experimental evidence of the anti-COVID-19 potential of honeybee propolis ingredients, caffeic acid phenethyl ester and artepillin c.
    PHYTOTHER RES. 2023 Feb 9. doi: 10.1002/ptr.7717
  • Huang, T; Sato, Y; Kuramochi, A; Ohba, Y; Sano, M; Miyagishi, M; Tateno, H; Wadhwa, R; Kawasaki, K; Uchida, T; Ekdahl, KN; Nilsson, B; Chung, UI; Teramura, Y.
    Surface modulation of extracellular vesicles with cell-penetrating peptide-conjugated lipids for improvement of intracellular delivery to endothelial cells.
    REGEN THER. 2023 Jan 11;22:90-98. doi: 10.1016/j.reth.2022.12.007
  • Yu, Y; Zhang, GX; Li, ZP; Wang, J; Liu, Y; Bhardwaj, R; Wadhwa, R; Nagao, Y; Shichiri, M; Gao, R.
    Designed fabrication of active tumor targeting covalent organic framework nanotherapeutics via a simple post-synthetic strategy.
    NANO RESEARCH. 03 January 2023. doi: 10.1007/s12274-022-5265-7
  • Limjanthong, N; Tohbaru, Y; Okamoto, T; Okajima, R; Kusama, Y; Kojima, H; Fujimura, A; Miyazaki, T; Kanamori, T; Sugiura, S; Ohnuma, K.
    Gravity-driven microfluidic device placed on a slow-tilting table enables constant unidirectional perfusion culture of human induced pluripotent stem cells.
    J BIOSCI BIOENG. 2022 Dec 29:S1389-1723(22)00346-2. doi: 10.1016/j.jbiosc.2022.11.007
  • Sugiura, S; Shin, K; Kanamori, T.
    Perfusion culture of endothelial cells under shear stress on microporous membrane in a pressure-driven microphysiological system.
    J BIOSCI BIOENG. 2022 Oct 14:S1389-1723(22)00258-4. doi: 10.1016/j.jbiosc.2022.09.005
  • Shefrin, S; Sari, AN; Kumar, V; Zhang, H; Meidinna, HN; Kaul, SC; Wadhwa, R; Sundar, D.
    Comparative computational and experimental analyses of some natural small molecules to restore transcriptional activation function of p53 in cancer cells harbouring wild type and p53(Ser46) mutant.
    CURR RES STRUCT BIOL. 2022 Sep 13;4:320-331. doi: 10.1016/j.crstbi.2022.09.002
  • Meidinna, HN; Shefrin, S; Sari, AN; Zhang, H; Dhanjal, JK; Kaul, SC; Sundar, D; Wadhwa, R.
    Identification of a new member of Mortaparib class of inhibitors that target mortalin and PARP1.
    FRONT CELL DEV BIOL. 2022 Sep 12;10:918970. doi: 10.3389/fcell.2022.918970
  • Sugiura, S; Satoh, T; Shin, K; Onuki-Nagasaki, R; Kanamori, T.
    Perfusion culture of multi-layered HepG2 hepatocellular carcinoma cells in a pressure-driven microphysiological system.
    J BIOSCI BIOENG. 2022 Aug 11:S1389-1723(22)00189-X. doi: 10.1016/j.jbiosc.2022.07.001
  • Tomita, T; Kawano, Y; Kassai, M; Onda, H; Nakajima, Y; Miyazaki, K.
    Hydroxy-β-sanshool isolated from Zanthoxylum piperitum (Japanese pepper) shortens the period of the circadian clock.
    FOOD FUNCT. 2022 Aug 12. doi: 10.1039/d2fo01036d
  • Yamagishi, A; Mizusawa, M; Uchida, K; Iijima, M; Kuroda, S; Fukazawa, K; Ishihara, K; Nakamura, C.
    Mechanical detection of interactions between proteins related to intermediate filament and transcriptional regulation in living cells.
    BIOSENS BIOELECTRON. 2022 Aug 6;216:114603. doi: 10.1016/j.bios.2022.114603
  • Yoon, AR; Wadhwa, R; Kaul, SC; Yun, CO.
    Why is Mortalin a Potential Therapeutic Target for Cancer.
    FRONT CELL DEV BIOL. 2022 Jun 29;10:914540. doi: 10.3389/fcell.2022.914540
  • Viswan, A; Yamagishi, A; Hoshi, M; Furuhata, Y; Kato, Y; Makimoto, N; Takeshita, T; Kobayashi, T; Iwata, F; Kimura, M; Yoshizumi, T; Nakamura, C.
    Microneedle Array-Assisted, Direct Delivery of Genome-Editing Proteins Into Plant Tissue.
    FRONT PLANT SCI. 2022 Jun 24;13:878059. doi: 10.3389/fpls.2022.878059
  • Nagasaki, A; Katoh, K; Hoshi, M; Doi, M; Nakamura, C; Uyeda, TQP.
    Characterization of phalloidin-negative nuclear actin filaments in U2OS cells expressing cytoplasmic actin-EGFP.
    GENES TO CELLS. 2022 Feb 23. doi: 10.1111/gtc.12930
  • Radhakrishnan, N; Kaul, SC; Wadhwa, R; Sundar, D.
    Phosphatidylserine Exposed Lipid Bilayer Models for Understanding Cancer Cell Selectivity of Natural Compounds: A Molecular Dynamics Simulation Study.
    MEMBRANES 12 (1);doi: 10.3390/membranes12010064 JAN
  • Malik, V; Radhakrishnan, N; Kaul, SC; Wadhwa, R; Sundar, D.
    Computational Identification of BCR-ABL Oncogenic Signaling as a Candidate Target of Withaferin A and Withanone.
    BIOMOLECULES 12 (2);doi: 10.3390/biom12020212 FEB
  • Sari, AN; Dhanjal, JK; Elwakeel, A; Kumar, V; Meidinna, HN; Zhang, H; Ishida, Y; Terao, K; Sundar, D; Kaul, SC; Wadhwa, R.
    A Low Dose Combination of Withaferin A and Caffeic Acid Phenethyl Ester Possesses Anti-Metastatic Potential In Vitro: Molecular Targets and Mechanisms.
    CANCERS (Basel). 2022 Feb 3;14(3):787. doi: 10.3390/cancers14030787
  • Penedo, M; Miyazawa, K; Okano, N; Furusho, H; Ichikawa, T; Alam, MS; Miyata, K; Nakamura, C; Fukuma, T.
    Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM.
    SCI ADV. 2021 Dec 24;7(52):eabj4990. doi: 10.1126/sciadv.abj4990. Epub 2021 Dec 22
  • Malik, V; Kumar, V; Kaul, SC; Wadhwa, R; Sundar, D.
    Potential of Withaferin-A, Withanone and Caffeic Acid Phenethyl ester as ATP-competitive inhibitors of BRAF: A bioinformatics study.
    CURR RES STRUCT BIOL. 2021 Nov 18;3:301-311. doi: 10.1016/j.crstbi.2021.11.004
  • Kaul, A; Kuthethur, R; Ishida, Y; Terao, K; Wadhwa, R; Kaul, SC.
    Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C.
    MOLECULES. 2021 Dec 23;27(1):80. doi: 10.3390/molecules27010080
  • Hori, K; Yoshimoto, S; Yoshino, T; Zako, T; Hirao, G; Fujita, S; Nakamura, C; Yamagishi, A; Kamiya, N.
    Recent advances in research on biointerfaces: From cell surfaces to artificial interfaces.
    J BIOSCI BIOENG. 2022 Jan 5:S1389-1723(21)00331-5. doi: 10.1016/j.jbiosc.2021.12.004
  • Radhakrishnan, N; Dhanjal, JK; Sari, AN; Ishida, Y; Terao, K; Kaul, SC; Sundar, D; Wadhwa, R.
    Caffeic acid phenethyl ester (CAPE) confers wild type p53 function in p53(Y220C) mutant: bioinformatics and experimental evidence.
    DISCOVER ONCOLOGY 12 (1);doi: 10.1007/s12672-021-00461-2 DEC 20
  • Wang, J; Zhang, H; Kaul, A; Li, K; Priyandoko, D; Kaul, SC; Wadhwa, R.
    Effect of Ashwagandha Withanolides on Muscle Cell Differentiation.
    BIOMOLECULES. 2021 Oct 4;11(10):1454. doi: 10.3390/biom11101454
  • Kumar, V; Sari, AN; Meidinna, HN; Dhanjal, JK; Subramani, C; Basu, B; Kaul, SC; Vrati, S; Sundar, D; Wadhwa, R.
    Computational and in vitro experimental analyses of the anti-COVID-19 potential of Mortaparib and MortaparibPlus.
    BIOSCI REP. 2021 Oct 29;41(10):BSR20212156. doi: 10.1042/BSR20212156
  • Bhargava, P; Mahanta, D; Kaul, A; Ishida, Y; Terao, K; Wadhwa, R; Kaul, SC.
    Experimental Evidence for Therapeutic Potentials of Propolis.
    NUTRIENTS. 2021 Jul 24;13(8):2528. doi: 10.3390/nu13082528
  • Kumar, A; Konar, A; Garg, S; Kaul, SC; Wadhwa, R.
    Experimental evidence and mechanism of action of some popular neuro-nutraceutical herbs.
    NEUROCHEMISTRY INTERNATIONAL 149; doi: 10.1016/j.neuint.2021.105124
  • Kaul, Z; Cheung, CTY; Bhargava, P; Sari, AN ; Yu, Y; He, HF; Bid, H; Henson, JD; Groden, J; Reddel, RR; Kaul, SC; Wadhwa, R.
    Functional characterization of miR-708 microRNA in telomerase positive and negative human cancer cells.
    SCI REP. 2021 Aug 23;11(1):17052. doi: 10.1038/s41598-021-96096-y
  • Elwakeel, A; Sari, AN; Dhanjal, JK; Meidinna, HN; Sundar, D; Kaul, SC; Wadhwa, R.
    Mutant p53(L194F) Harboring Luminal-A Breast Cancer Cells Are Refractory to Apoptosis and Cell Cycle Arrest in Response to Mortaparib(Plus), a Multimodal Small Molecule Inhibitor.
    CANCERS (Basel). 2021 Jun 18;13(12):3043. doi: 10.3390/cancers13123043
  • Yamagishi, A; Ito, F; Nakamura, C.
    Study on Cancer Cell Invasiveness via Application of Mechanical Force to Induce Chloride Ion Efflux.
    ANAL CHEM. 2021 Jun 21. doi: 10.1021/acs.analchem.1c01589
  • Tomita, T; Wadhwa, R; Kaul, SC; Kurita, R; Kojima, N; Onishi, Y.
    Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa.
    J NAT PROD. 2021 Jun 21. doi: 10.1021/acs.jnatprod.0c01276
  • Dhanjal, JK; Kumar, V; Garg, S; Subramani, C; Agarwal, S; Wang, J; Zhang, H; Kaul, A; Kalra, RS; Kaul, SC; Vrati, S; Sundar, D; Wadhwa, R
    Molecular mechanism of anti-SARS-CoV2 activity of Ashwagandha-derived withanolides
    Int J Biol Macromol. 2021 Jun 9:S0141-8130(21)01215-0. doi: 10.1016/j.ijbiomac.2021.06.015
  • Li, X; Wang, J; Zhang, H; Xiao, L; Lei, Z; Kaul, SC; Wadhwa, R; Zhang, Z.
    Low Dose of Fluoride in the Culture Medium of Cordyceps militaris Promotes Its Growth and Enhances Bioactives with Antioxidant and Anticancer Properties.
    J FUNGI (Basel). 2021 Apr 28;7(5):342. doi: 10.3390/jof7050342
  • Penedo, M; Shirokawa, T; Alam, MS; Miyazawa, K; Ichikawa, T; Okano, N; Furusho, H; Nakamura, C; Fukuma, T.
    Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells.
    SCI REP. 2021 Apr 8;11(1):7756. doi: 10.1038/s41598-021-87319-3
  • Kalra, RS; Kumar, V; Dhanjal, JK; Garg, S; Li, X; Kaul, SC; Sundar, D; Wadhwa, R.
    COVID19-inhibitory activity of withanolides involves targeting of the host cell surface receptor ACE2: insights from computational and biochemical assays.
    J BIOMOL STRUCT DYN. 2021 Apr 2:1-14. doi: 10.1080/07391102.2021.1902858
  • Sari AN, Elwakeel A, Dhanjal JK,Kumar V, Sundar D, Kaul SC, Wadhwa R
    Identification and Characterization of Mortaparib(Plus)-A Novel Triazole Derivative That Targets Mortalin-p53 Interaction and Inhibits Cancer-Cell Proliferation by Wild-Type p53-Dependent and -Independent Mechanisms.
    CANCERS (Basel). 2021 Feb 17;13(4):835. doi: 10.3390/cancers13040835
  • Malik V, Kumar V, Kaul SC, Wadhwa R, Sundar D
    Computational Insights into the Potential of Withaferin-A, Withanone and Caffeic Acid Phenethyl Ester for Treatment of Aberrant-EGFR Driven Lung Cancers.
    BIOMOLECULES. 2021 Jan 26;11(2):160. doi: 10.3390/biom11020160
  • Maejima A, Ishibashi K, Kim H, Kumagai I, Asano R
    Evaluation of intercellular cross-linking abilities correlated with cytotoxicities of bispecific antibodies with domain rearrangements using AFM force-sensing.
    BIOSENS BIOELECTRON. 2021 Jan 27;178:113037. doi: 10.1016/j.bios.2021.113037
  • Wadhwa R, Yadav NS, Katiyar SP, Yaguchi T, Lee C, Ahn H, Yun CO, Kaul SC, Sundar D
    Molecular dynamics simulations and experimental studies reveal differential permeability of withaferin-A and withanone across the model cell membrane.
    SCI REP. 2021 Jan 27;11(1):2352. doi: 10.1038/s41598-021-81729-z
  • Landeira JM, Liu BB, Omura T, Akiba T, Tanaka Y
    Salinity effects on the first larval stage of the invasive crab Hemigrapsus takanoi: Survival and swimming patterns
    ESTUARINE COASTAL AND SHELF SCIENCE 245; (2020) doi: 10.1016/j.ecss.2020.106976
  • Konar A, Kalra RS, Chaudhary A, Nayak A, Guruprasa KP, Satyamoorthy K, Ishida Y, Terao K, Kaul SC, Wadhwa R
    Identification of Caffeic Acid Phenethyl Ester (CAPE) as a Potent Neurodifferentiating Natural Compound That Improves Cognitive and Physiological Functions in Animal Models of Neurodegenerative Diseases
    Front Aging Neurosci. 2020 Nov 10;12:561925. doi: 10.3389/fnagi.2020.561925
  • Nishihara R, Niwa K, Tomita T, Kurita R
    Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence
    Bioconjug Chem. 2020 Nov 25. doi: 10.1021/acs.bioconjchem.0c00536
  • Kim H, Ishibashi K, Iijima M, Kuroda S, Nakamura C
    Influence of Nivolumab for Intercellular Adhesion Force between a T Cell and a Cancer Cell Evaluated by AFM Force Spectroscopy
    Sensors (Basel) 20(19):E5723. (2020)doi: 10.3390/s20195723
  • Priyanka, Wadhwa R, Chaudhuri R, Nag TC, Seth P
    Novel role of mortalin in attenuating HIV-1 Tat-mediated astrogliosis
    J Neuroinflammation.;17(1):276. (2020) doi: 10.1186/s12974-020-01912-3
  • Yu Y, Yang X, Reghu S, Kaul SC, Wadhwa R, Miyako E
    Photothermogenetic inhibition of cancer stemness by near-infrared-light-activatable nanocomplexes
    Nat Commun. 11:4117 (2020) doi: 10.1038/s41467-020-17768-3
  • Wang J, Bhargava P, Yu Y, Sari AN, Zhang H, Ishii N, Yan K, Zhang Z, Ishida Y, Terao K, Kaul SC, Miyako E, Wadhwa R
    Novel Caffeic Acid Phenethyl Ester-Mortalin Antibody Nanoparticles Offer Enhanced Selective Cytotoxicity to Cancer Cells
    Cancers (Basel). 12(9):E2370. (2020) doi: 10.3390/cancers12092370
  • Malik V, Garg S, Afzal S, Dhanjal JK, Yun CO, Kaul SC, Sundar D, Wadhwa R
    Bioinformatics and Molecular Insights to Anti-Metastasis Activity of Triethylene Glycol Derivatives
    Int J Mol Sci. 21(15):E5463. (2020) doi: 10.3390/ijms21155463
  • Kalra RS, Tomar D, Meena AS, Kandimalla R
    SARS-CoV-2, ACE2, and Hydroxychloroquine: Cardiovascular Complications, Therapeutics, and Clinical Readouts in the Current Settings
    Pathogens. 9: 0 (2020) doi: 10.3390/pathogens9070546
  • Kumar V, Dhanjal JK, Bhargava P, Kaul A, Wang J, Zhang H, Kaul SC, Wadhwa R, Sundar D
    Withanone and Withaferin-A are predicted to interact with transmembrane protease serine 2 (TMPRSS2) and block entry of SARS-CoV-2 into cells
    J. Biomolecular Structure 21: 0 (2020) doi: 10.1080/07391102.2020.1775704
  • Asano K, Kim HJ, Sakaki K, Nakamura YM, Wang YM, Isobe S, Doi M, Fujita A, Maejima N, Machida A, Watanuki T, Westerwaal RJ, Schreuders H, Dam B
    Metallurgical Synthesis of Mg2FexSi1-x Hydride: Destabilization of Mg2FeH6 Nanostructured in Templated Mg2Si
    Inorganic Chemistry 59: 2758-2764 (2020) doi: 10.1021/acs.inorgchem.9b03117
  • Kumar V, Dhanjal JK, Bhargava P, Kaul A, Wang J, Zhang H, Kaul SC, Wadhwa R, Sundar D.
    Withanone and withaferin-A Are Predicted to Interact with Transmembrane Protease Serine 2 (TMPRSS2) and Block Entry of SARS-CoV-2 Into Cells
    J Biomol Struct Dyn. 1-27 (2020) doi: 10.1080/07391102.2020.1775704
  • Kumar V, Dhanjal JK, Kaul SC, Wadhwa R, Sundar D
    Withanone and caffeic acid phenethyl ester are predicted to interact with main protease (Mpro) of SARS-CoV-2 and inhibit its activity
    J Biomol Struct Dyn 1-13 (2020) doi: 10.1080/07391102.2020.1772108
  • Kim H, Hoshi M, Iijima M, Kuroda S, Nakamura C
    Development of a universal method for the measurement of binding affinities of antibody drugs towards a living cell based on AFM force spectroscopy
    Anal Methods (2020) doi: 10.1039/d0ay00788a
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    Combination of Withaferin-A and CAPE Provides Superior Anticancer Potency: Bioinformatics and Experimental Evidence to Their Molecular Targets and Mechanism of Action
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    Soyasapogenol-A targets CARF and results in suppression of tumor growth and metastasis in p53 compromised cancer cells
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    Stress-induced changes in CARF expression determine cell fate to death, survival, or malignant transformation
    Cell Stress Chaperones 25(3): 481-494 (2020). doi: 10.1007/s12192-020-01088-y
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    Protein microswimmers capable of delivering cells for tissue engineering applications
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    Anti-stress, Glial- and Neuro-differentiation Potential of Resveratrol: Characterization by Cellular, Biochemical and Imaging Assays
    Nutrients 12(3): 671 (2020). doi: 10.3390/nu12030671
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    Adhesion strengths between cancer cells with different malignancies and endothelial cells measured using atomic force microscopy
    Jpn J Appl Phys 59: SDDE01 (2020) doi: 10.7567/1347-4065/ab51ad
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    Induction of Senescence in Cancer Cells by a Novel Combination of Cucurbitacin B and Withanone: Molecular Mechanism and Therapeutic Potential
    J Gerontol A Biol Sci Med Sci. 75(6):1031-1041 (2020). doi: 10.1093/gerona/glz077
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    Mortaparib, a novel dual inhibitor of mortalin and PARP1, is a potential drug candidate for ovarian and cervical cancers
    J Exp Clin Cancer Res. 38(1): 499 (2019). doi: 10.1186/s13046-019-1500-9
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    beta-Arrestin 1 (ARRB1) serves as a molecular marker of the peripheral circadian rhythm
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    2, 3-Dihydro-3beta-methoxy Withaferin-A Lacks Anti-Metastasis Potency: Bioinformatics and Experimental Evidences
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    Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro
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    M1 muscarinic receptor is a key target of neuroprotection, neuroregeneration and memory recovery by i-Extract from Withania somnifera
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    Quantitative Measurements of Intercellular Adhesion Strengths between Cancer Cells with Different Malignancies Using Atomic Force Microscopy
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    Folic Acid Receptor-Mediated Targeting Enhances the Cytotoxicity, Efficacy, and Selectivity of Withania somnifera Leaf Extract: In vitro and in vivo Evidence
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    Marine Carotenoid Fucoxanthin Possesses Anti-Metastasis Activity: Molecular Evidence
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    Modulation of Diacylglycerol-Induced Melanogenesis in Human Melanoma and Primary Melanocytes: Role of Stress Chaperone Mortalin
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    The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis
    INTERNATIONAL J. Biological Sciences 15: 1546-1556 (2019) doi: 10.7150/ijbs.33423
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    TAS0728, A Covalent-binding, HER2-selective Kinase Inhibitor Shows Potent Antitumor Activity in Preclinical Models
    Mol Cancer Ther. 18: 733-742 (2019) doi: 10.1158/1535-7163. MCT-18-1085
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    Proteomics to Predict Loss of RXR-gamma During Progression of Epithelial Ovarian Cancer
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    Rat Glioma Cell-Based Functional Characterization of Anti-Stress and Protein Deaggregation Activities in the Marine Carotenoids, Astaxanthin and Fucoxanthin
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    Wild type p53 function in p53(Y220C) mutant harboring cells by treatment with Ashwagandha derived anticancer withanolides: bioinformatics and experimental evidence
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    Molecular Insights into Withaferin-A-Induced Senescence: Bioinformatics and Experimental Evidence to the Role of NFkappaB and CARF
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    Anti-Stress and Glial Differentiation Effects of a Novel Combination of Cucurbitacin B and Withanone (CucWi-N): Experimental Evidence
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    Anticancer Activity in Honeybee Propolis: Functional Insights to the Role of Caffeic Acid Phenethyl Ester and Its Complex With gamma-Cyclodextrin
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    TAS6417, A Novel EGFR Inhibitor Targeting Exon 20 Insertion Mutations
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    Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence
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    A genome editing vector that enables easy selection and identification of knockout cells
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    Self-assembled nanodiamond supraparticles for anticancer chemotherapy
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    CARF enrichment promotes epithelial-mesenchymal transition via Wnt/beta-catenin signaling: its clinical relevance and potential as a therapeutic target
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    Cucurbitacin B and cancer intervention: Chemistry, biology and mechanisms (Review
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    Tumor suppressor activity of miR-451: Identification of CARF as a new target
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    2,3-Dihydro-3beta-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity
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    Mortalin deficiency suppresses fibrosis and induces apoptosis in keloid spheroids
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    Induction of senescence in cancer cells by 5-Aza-2-deoxycytidine: Bioinformatics and experimental insights to its targets
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    CARF is a multi-module regulator of cell proliferation and a molecular bridge between cellular senescence and carcinogenesis
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    Quantitative measurements of intercellular adhesion between a macrophage and cancer cells using a cup-attached AFM chip
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    Epigenetic regulation of the circadian clock: role of 5-aza-2-deoxycytidine
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    The Position of the GFP Tag on Actin Affects the Filament Formation in Mammalian Cells
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    Addressing Challenges to Enhance the Bioactives of Withania somnifera through Organ, Tissue, and Cell Culture Based Approaches
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    Withaferin-A kills cancer cells with and without telomerase: chemical, computational and experimental evidences
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    Relevance of Mortalin to Cancer Cell Stemness and Cancer Therapy
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