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研究課題

多くの可能性を秘めた脂肪組織由来細胞を使った再生医療応用技術を開発しています。私たちは、脂肪幹細胞等を用いた腱やケロイド、軟部組織の再建を目指し、効率的な細胞誘導法開発や移植による治療法開発に挑んでいます。

研究課題

末梢神経系と様々な臓器をin vitroで接続することで、創薬アッセイ系への応用を目指しています。また、神経堤細胞・末梢神経の新規誘導技術の開発を行っており、特に自律神経系（交感/副交感神経）の誘導技術開発に注力しています。

研究課題

組織培養デバイスを用いて人工組織に大きな主血管とそこから枝分かれする毛細血管を作製する技術を研究している。酸素や栄養を供給して大きな組織を維持したり、試験の対象である薬剤を流し入れたりすることができる。

研究課題

共焦点ラマン分光顕微鏡を生体組織由来の細胞に応用し、非侵襲かつ高精度に細胞プロファイリング・分画する技術を開発する。この技術は加工細胞の新型医薬品開発が可能となり、革新的な技術の発展に寄与するものと期待できる。

研究課題

私たちは臨床の固形がんを再現するin vitroがん微小環境実験法と、豊富な間質増生を伴う臨床組織像を再構築する担がんマウスモデルを開発しました。これらを用いて新たな医療シーズとその非臨床POCを取得することを目指しています。

研究課題

免疫系は外来の異物を認識し排除するのみならず神経系、代謝系、がんとの相互作用を通じて様々な疾患に関係しています。この免疫応答の理解を目指し、その情報処理機構を実験、情報科学、数理の多方面から研究しています。

研究課題

微細加工技術を利用して生理学性の高い培養環境を創出し、高次の細胞・臓器機能を発現可能な生体模倣システム（Microphysiological systems、MPS）の開発と産業応用を目指します。

メンバー

業績リスト

• Miyazaki Y, Oda T, Inagaki Y, Kushige H, Saito Y, Mori N, Takayama Y, Kumagai Y, Mitsuyama T, Kida YS
  Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.
  SCI REP. 2021 Feb 25;11(1):4690. doi: 10.1038/s41598-021-84058-3
• Kumagai Y, Hirasawa T, Wachi M
  Requirement of the LtsA Protein for Formation of the Mycolic Acid-Containing Layer on the Cell Surface of Corynebacterium glutamicum.
  MICROORGANISMS. 2021 Feb 16;9(2):409. doi: 10.3390/microorganisms9020409
• Shinohara M, Arakawa H, Oda Y, Shiraki N, Sugiura S, Nishiuchi T, Satoh T, Iino K, Leo S, Kato Y, Araya K, Kawanishi T, Nakatsuji T, Mitsuta M, Inamura K, Goto T, Shinha K, Nihei W, Komori K, Nishikawa M, Kume S, Kato Y, Kanamori T, Sakai Y, Kimura H
  Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system
  Sci Rep. 2021 Mar 8;11(1):5437. doi: 10.1038/s41598-021-84861-y
• Baba T, Takagi T, Sumaru K, Kanamori T, Dewa T, Nango M
  Membrane properties of ether-type phosphatidylcholine bearing partially fluorinated C(18)-monoacetylenic chains and their applicability to membrane protein reconstitution matrices
  Colloids Surf B Biointerfaces. 2020 Nov 13:111459. doi: 10.1016/j.colsurfb.2020.111459
• Figarol A, Piantino M, Furihata T, Satoh T, Sugiura S, Kanamori T, Matsusaki M
  Interstitial flow regulates in vitro three-dimensional self-organized brain micro-vessels
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 533 (3) 600-606; (2020) doi:10.1016/j.bbrc.2020.09.061 DEC 10
• Takayama Y, Akagi Y, Shibuya Y, Kida YS
  Exposure to small molecule cocktails allows induction of neural crest lineage cells from human adipose-derived mesenchymal stem cells
  PLoS One. ;15(10):e0241125. (2020)doi: 10.1371/journal.pone.0241125
• Miyazaki Y, Oda T, Mori N, Kida YS
  Adipose-derived mesenchymal stem cells differentiate into pancreatic cancer-associated fibroblasts in vitro
  FEBS Open Bio. 2020 Sep 15. (2020) doi: 10.1002/2211-5463.12976
• Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
  Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
  ROYAL SOCIETY OPEN SCIENCE 7 (7); (2020) doi: 10.1098/rsos.200027
• Mori N, Kida YS
  Expression of genes involved in drug metabolism differs between perfusable 3D liver tissue and conventional 2D-cultured hepatocellular carcinoma cells
  FEBS Open Bio. 0 (2020) Aug 14. doi: 10.1002/2211-5463.12948
• Maruyama K, Kidoya H, Takemura N, Sugisawa E, Takeuchi O, Kondo T, Eid MMA, Tanaka H, Martino MM, Takakura N, Takayama Y, Akira S, Vandenbon A, Kumagai Y
  Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
  Cell Rep. 32: 107906 (2020) doi:10.1016/j.celrep.2020.107906
• Sugisawa E, Takayama Y, Takemura N, Kondo T, Hatakeyama S, Kumagai Y, Sunagawa M, Tominaga M, Maruyama K
  RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis
  Cell. 8674: 30754-6 (2020) doi:10.1016/j.cell.2020.06.022
• Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
  Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
  Royal Society Open Science 7 7: 0 (2020) doi:10.1098/rsos.200027
• Watanabe U,Sugiura S, Kakehata M, Yanagawa F, Takagi T, Sumaru K, Satoh T, Tamura M, Hosokawa Y, Torizuka K, Kanamori T
  Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering
  Micromachines 11: 0 (2020) doi:10.3390/mi11070679
• Takayama Y, Kushige H, Akagi Y, Suzuki Y, Kumagai Y, Kida YS^*
  Selective induction of Human Autonomic neurons enables precise control of cardiomyocyte Beating
  Sci Rep.10: 9464 (2020) doi:10.1038/s41598-020-66303-3
• Mori N, Akagi Y, Imai Y, Takayama Y, Kida YS^*
  Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue
  Sci Rep. 10: 5646 (2020) doi: 10.1038/s41598-020-62286-3
• Baba T, Takagi T, Sumaru K, Kanamori T
  Effect of the fluorination degree of partially fluorinated octyl-phosphocholine surfactants on their interfacial properties and interactions with purple membrane as a membrane protein model
  Chemistry And Physics Lipids 227 0: 0 (2020) doi: 10.1016/j.chemphyslip.2020.104870
• Marx U, Akabane T, Andersson TB, Baker E, Beilmann M, Beken S, Brendler-Schwaab S, Cirit M, David R, Dehne EM, Durieux I, Ewart L, Fitzpatrick SC, Frey O, Fuchs F, Griffith LG, Hamilton GA, Hartung T, Hoeng J, Hogberg H, Hughes DJ, Ingber DE, Iskandar A, Kanamori T, Kojima H, Kuehnl J, Leist M, Li B, Loskill P, Mendrick DL, Neumann T, Pallocca G, Rusyn I, Smirnova L, Steger-Hartmann T, Tagle DA, Tonevitsky A, Tsyb S, Trapecar M, Van de Water B, Van den Eijnden-van Raaij J, Vulto P, Watanabe K, Wolf A, Zhou X, Roth A
  Biology-inspired microphysiological systems to advance patient benefit and animal welfare in drug development
  Altex. 12: 0 (2020) doi: 10.14573/altex.2001241
• Arakawa H, Sugiura S, Kawanishi T, Shin K, Toyoda H, Satoh T, Sakai Y, Kanamori T, Kato Y
  Kinetic analysis of sequential metabolism of triazolam and its extrapolation to humans using an entero-hepatic two-organ microphysiological system
  Lab Chip. 3: 25 (2020) doi: 10.1039/c9lc00884e
• Komatsu H, Sakai K, Baba T, Takagi T, Kanamori T
  Aggregation behavior of short-chained archaeal phospholipid analogs: Contribution methyl branches to lipid hydrophobicity and membrane formability
  Colloid And Interface Science Communications 129: 3827-3832 (2019) doi: 10.1016/j.colcom.2019.100200
• Hirama H, Satoh T, Sugiura S, Shin K, Onuki-Nagasaki R, Kanamori T, Inoue T
  Glass-based organ-on-a-chip device for restricting small molecular absorption
  J Biosci Bioeng. 127: 641-646 (2019) doi: 10.1016/j.jbiosc.2018.10.019
• Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
  Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center
  J. Physical Chemistry A 123: 3985-3991 (2019) doi: 10.1021/acs.jpca.8b11613
• Ishida S, Kanamori T
  Microphysiological system as a promising technology for drug assay
  Nihon Yakurigaku Zasshi. 154: 345-351 (2019) doi: 10.1254/fpj.154.345
• Kise R, Fukumi A, Shioya N, Shimoaka T, Sonoyama M, Amii H, , Takagi T, Kanamori T, Eda K, Hasegawa T
  Fluorous Property of a Short Perfluoroalkyl-Containing Compound Realized by Self-Assembled Monolayer Technique on a Silicon Substrate
  Bulletin The Chemical Society Japan 92: 785-789 (2019) doi: 10.1246/bcsj.20180356
• Tsukamoto T, Sogo T, Ueyama T, Nakao S, Harada Y, Ihara D, Akagi, Y, Kida YS, Hasegawa K, Nagamune T, Kawahara M*, Kawamura T*
  Chimeric G-CSF Receptor-Mediated STAT3 Activation Contributes to Efficient Induction of Cardiomyocytes from Mouse Induced Pluripotent Stem Cells
  Biotechnology J. 93: 10 (2019) doi: 10.1002/biot.201900052
• Takahashi H, Yoshino M, Morita K, Takagi T, Yokoyama Y, Kikukawa T, Amii H, Kanamori T, Sonoyama M
  Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
  Biochim Biophys Acta Biomembr. 1861: 631-642 (2019) doi: 10.1016/j.bbamem.2018.12.015
• Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
  Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
  Commun Biol. 1: 218 (2018) doi: 10.1038/s42003-018-0222-4
• Shibuta M, Tamura M, Kanie K, Yanagisawa M, Matsui H, Satoh T, , Takagi T, Kanamori T, Sugiura S, Kato R
  Imaging cell picker: A morphology-based automated cell separation system on a photodegradable hydrogel culture platform
  J. Bioscience And Bioengineering 126: 653-660 (2018) doi: 10.1016/j.jbiosc.2018.05.004
• Mori N, Morimoto Y, Takeuchi S*
  Perfusable and stretchable 3D culture system for skin-equivalent
  Biofabrication. 11: 011001 (2018) doi: 10.1088/1758-5090/aaed12
• Vandenbon A^#, Kumagai Y^#, Lin M, Suzuki Y, Nakai K^*
  Waves of chromatin modifications in mouse dendritic cells in response to LPS stimulation
  Genome Biol. 19: 138 (2018) doi: 10.1186/s13059-018-1524-z
• Tashiro S, Le MNT, Kusama Y, Nakatani E, Suga M, Furue MK, Satoh T, Sugiura S, Kanamori T, Ohnuma K
  High cell density suppresses BMP4-induced differentiation of human pluripotent stem cells to produce macroscopic spatial patterning in a unidirectional perfusion culture chamber
  J Biosci Bioeng. 126: 379-388 (2018) doi: 10.1016/j.jbiosc.2018.03.007
• Maruyama K^*, Takayama Y, Sugisawa E, Yamanoi Y, Yokawa T, Kondo T, Ishibashi KI, Sahoo BR, Takemura N, Mori Y, Kanemaru H, Kumagai Y, Martino MM, Yoshioka Y, Nishijo H, Tanaka H, Sasaki A, Ohno N, Iwakura Y, Moriyama Y, Nomura M, Akira S, Tominaga M*
  The ATP Transporter VNUT Mediates Induction of Dectin-1-Triggered Candida Nociception
  iScience. 0: 0 (2018) doi: 10.1016/j.isci.2018.08.007
• Wagatsuma A^*, Takayama Y, Hoshino T, Shiozuka M, Yamada S, Matsuda R, Mabuchi K
  Pharmacological targeting of HSP90 with 17-AAG induces apoptosis of myogenic cells through activation of the intrinsic pathway
  Mol Cell Biochem. 163: 170-182 (2018) doi: 10.1007/s11010-017-3250-3
• Sumaru K, Takagi T, Morishita K, Satoh T, Kanamori T
  Fabrication of pocket-like hydrogel microstructures through photolithography
  St Matter 14: 5710-5714 (2018) doi: 10.1039/c8sm00865e
• Sumaru K, Takagi T, Morishita K, Kanamori T
  Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
  Biomacromolecules. 19: 2913-2922 (2018) doi: 10.1021/acs.biomac.8b00470
• Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
  Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
  Commun Biol. 0: 0 (2018) doi: 10.1038/s42003-018-0222-4
• Kanamori T, Sugiura S, Sakai Y
  Technical aspects of microphysiological systems (MPS) as a promising wet human-in-vivo simulator
  Drug Metab Pharmacokinet. 33: 40-42 (2018) doi: 10.1016/j.dmpk.2017.11.006
• Sumaru K, Takagi T, Satoh T, Kanamori T
  Photo- and Thermoresponsive Dehydration of Spiropyran-Functionalized Polymer Regulated by Molecular Recognition
  Macromol Rapid Commun. 39: 0 (2018) doi: 10.1002/marc.201700234
• Ito N, Katoh K, Kushige H, Saito Y, Umemoto T, Matsuzaki Y, Kiyonari H, Kobayashi D, Soga M, Era T, Araki N, Furuta Y, Suda T, Kida Y, Ohta K^*
  Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency
  Sci Rep. 8: 1634 (2018) doi: 10.1038/s41598-018-20057-1
• Ueyama T, Nakao S, Tsukamoto T, Ihara D, Harada Y, Akagi Y, Nakagawa S, Kida YS, Sogo T and Kawamura T^*
  PTEN/Akt Axis is Involved in Somatic Cell Reprogramming to Mouse iPS Cells
  Biomed J Sci & Tech. Res. 11: 5 (2018) DOI: 10.26717/BJSTR.2018.11.002162
• Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
  Study of Perfluoroalkyl Chain-Specific Band Shift in Infrared Spectra on the Chain Length
  J. Physical Chemistry A 121: 8425-8431 (2017) doi: 10.1021/acs.jpca.7b08940
• Kondo Y, Hattori K, Tashiro S, Nakatani E, Yoshimitsu R, Satoh T, Sugiura S, Kanamori T, Ohnuma K
  Compartmentalized microfluidic perfusion system to culture human induced pluripotent stem cell aggregates
  J Biosci Bioeng. 124: 234-241 (2017) doi: 10.1016/j.jbiosc.2017.03.014
• Sumaru K, Morishita K, Takagi T, Satoh T, Kanamori T
  Sectioning of cultured cell monolayer using photo-acid-generating substrate and micro-patterned light projection
  European Polymer J. 121: 16964-16969 (2017) doi: 10.1016/j.eurpolymj.2017.02.021
• Watanabe Y, Miyasaka KY, Kubo A, Kida YS, Nakagawa O, Hirate Y, Sasaki H, Ogura T^*
  Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm
  Sci Rep. 0: 0 (2017) doi: 10.1038/srep46135
• Kondo Y, Yada Y, Haga T, Takayama Y, Isomura T, Jimbo Y, Fukayama O, Hoshino T^*, Mabuchi K
  Temporal relation between neural activity and neurite pruning on a numerical model and a microchannel device with micro electrode array
  Biochem Biophys Res Commun. 486: 539-544 (2017) doi: 10.1016/j.bbrc.2017.03.082
• Tamura M, Sugiura S, Takagi T, Satoh T, Sumaru K, Kanamori T, Okada T, Matsui H
  Morphology-based optical separation of subpopulations from a heterogeneous murine breast cancer cell line
  PloS One. 12: 0 (2017) doi: 10.1371/journal.pone.0179372
• Takayama Y^#, Wakabayashi T#, Kushige H, Saito Y, Shibuya Y, Shibata S, Akamatsu W, Okano H, Kida YS^*
  Brief Exposure to Small Molecules Allows Induction of Mouse Embryonic Fibroblasts Into Neural Crest-Like Precursors
  FEBS Lett. 591: 590-602 (2017) doi: 10.1002/1873-3468.12572
• Satoh T^#, Sugiura S^#*, Shin K, Onuki-Nagasaki R, Ishida S, Kikuchi K, Kakiki M, Kanamori T
  A Multi-Throughput Multi-Organ-On-A-Chip System on a Plate Formatted Pneumatic Pressure-Driven Medium Circulation Platform
  Lab Chip. 18:115-125 (2017) doi: 10.1039/c7lc00952f