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ステムセルバイオテクノロジー研究グループ
研究概要
研究開発：幹細胞工学は再生医療だけでなく、創薬や食品開発など様々な研究分野に貢献することができます。当グループでは幹細胞を使った細胞誘導法や、機能的システムの生体外再構築法の研究開発を行っています。
技術支援：当研究グループでは、基盤的研究に加えて、民間企業や大学・研究機関からのご要望に応じた共同研究や技術コンサルティングを行っています。
研究課題
研究課題1:生体外自律神経ネットワークシステムの確立
研究担当者:木田 泰之、髙山 祐三、赤木 祐香、二橋 佑磨
例えば、食品は自律神経系である迷走神経を介して脳機能や全身の生理機能をコントロールしています。このような自律神経機能は交感神経と副交感神経のバランスに依存してます。動物愛護の観点から、身体の生理機能を生体外で再現する動物実験代替法の開発ニーズが高まっています。我々はヒト幹細胞からの交感神経と副交感神経を誘導する方法を開発しました。本研究課題では脳腸相関などの生体の迷走神経路を模倣する動物実験代替法とその評価法を開発します。
研究課題
研究課題2:がん微小環境再現モデルによる革新的がん治療法の開発
研究担当者:木田 泰之、熊谷 雄太郎、森 宣仁
難治性固形がんである膵癌では、がん間質の線維化が障壁となり、治療薬が腫瘍の深部にまで浸透しないという間質バリアが治療の課題です。我々は、がん関連線維芽細胞や免疫細胞で構成されるがん微小環境を研究するための実験系構築を進めてきました。本研究課題では、がんの基盤研究や治療法開発、診断に向けた細胞誘導法や3次元組織構築法を開発します。
メンバー
| 名前 | 役職 | 主な研究テーマ |
|---|---|---|
| 木田 泰之 KIDA Yasuyuki | グループ長 | ・ステムセルバイオテクノロジー ・実験モデルの作製 ・幹細胞の再生医療応用 |
| 髙山 祐三 TAKAYAMA Yuzo | 主任研究員 | ・細胞誘導・加工技術の開発 ・幹細胞由来人工組織の作製 ・微細加工技術を用いた細胞組織間の相互作用評価系構築 |
| 熊谷 雄太郎 KUMAGAI Yutaro | 主任研究員 | ・自然免疫、がん免疫の分子レベルでの解明 ・免疫応答のバイオインフォマティクス ・免疫系動作原理の数理的理解と応用 |
| 森 宣仁 MORI Nobuhito | 主任研究員 | ・血管付き3次元肝臓組織の開発 ・3次元組織灌流システムの開発 ・血管付き3次元がんモデルの開発 |
| 赤木 祐香 AKAGI Yuuka | 研究員 | ・非破壊・非侵襲的な細胞評価技術の開発 ・細胞・組織間の相互作用評価 ・ティッシュエンジニアリング技術による大脳組織の作製 |
| 二橋 佑磨 NIHASHI Yuuma | 研究員 | ・幹細胞分化のバイオインフォマティクス ・体細胞のダイレクトリプログラミング法の開発 ・実験動物代替法の構築 |
業績リスト
- Nohira, N; Shinji, S; Nakamura, S; Nihashi, Y; Shimosato, T; Takaya, T.
Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells.
BIOMEDICINES. 2022 Oct 25;10(11):2691. doi: 10.3390/biomedicines10112691 - Imai, Y;Mori, N; Nihashi, Y; Kumagai, Y; Shibuya, Y; Oshima, J; Sasaki, M; Sasaki, K; Aihara, Y; Sekido, M; Kida, YS.
Therapeutic Potential of Adipose Stem Cell-Derived Conditioned Medium on Scar Contraction Model.
BIOMEDICINES. 2022 Sep 24;10(10):2388. doi: 10.3390/biomedicines10102388 - Sugisawa, E; Kondo, T; Kumagai, Y; Kato, H; Takayama, Y; Isohashi, K; Shimosegawa, E; Takemura, N; Hayashi, Y; Sasaki, T; Martino, MM; Tominaga, M; Maruyama, K.
Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
CELL REP. 2022 Mar 8;38(10):110462. doi: 10.1016/j.celrep.2022.110462 - Miyazaki, Y; Mori, N; Akagi, Y; Oda, T; Kida, YS.
Potential Metabolite Markers for Pancreatic Cancer Identified by Metabolomic Analysis of Induced Cancer-Associated Fibroblasts.
CANCERS. 2022 Mar 8;14(6):1375. doi: 10.3390/cancers14061375 - Akagi, Y; Mori, N; Kawamura, T; Takayama, Y; Kida, YS.
Non-invasive cell classification using the Paint Raman Express Spectroscopy System (PRESS)
SCI REP. 2021 Apr 23;11(1):8818. doi: 10.1038/s41598-021-88056-3 - Mori, N; Kida, YS.
Applicability of Artificial Vascularized Liver Tissue to Proteomic Analysis.
MICROMACHINES (Basel). 2021 Apr 11;12(4):418. doi: 10.3390/mi12040418 - Miyazaki Y, Oda T, Inagaki Y, Kushige H, Saito Y, Mori N, Takayama Y, Kumagai Y, Mitsuyama T, Kida YS
Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.
SCI REP. 2021 Feb 25;11(1):4690. doi: 10.1038/s41598-021-84058-3 - Kumagai Y, Hirasawa T, Wachi M
Requirement of the LtsA Protein for Formation of the Mycolic Acid-Containing Layer on the Cell Surface of Corynebacterium glutamicum.
MICROORGANISMS. 2021 Feb 16;9(2):409. doi: 10.3390/microorganisms9020409 - Baba T, Takagi T, Sumaru K, Kanamori T, Dewa T, Nango M
Membrane properties of ether-type phosphatidylcholine bearing partially fluorinated C(18)-monoacetylenic chains and their applicability to membrane protein reconstitution matrices
Colloids Surf B Biointerfaces. 2020 Nov 13:111459. doi: 10.1016/j.colsurfb.2020.111459 - Takayama Y, Akagi Y, Shibuya Y, Kida YS
Exposure to small molecule cocktails allows induction of neural crest lineage cells from human adipose-derived mesenchymal stem cells
PLoS One. ;15(10):e0241125. (2020)doi: 10.1371/journal.pone.0241125 - Miyazaki Y, Oda T, Mori N, Kida YS
Adipose-derived mesenchymal stem cells differentiate into pancreatic cancer-associated fibroblasts in vitro
FEBS Open Bio. 2020 Sep 15. (2020) doi: 10.1002/2211-5463.12976 - Mori N, Kida YS
Expression of genes involved in drug metabolism differs between perfusable 3D liver tissue and conventional 2D-cultured hepatocellular carcinoma cells
FEBS Open Bio. 0 (2020) Aug 14. doi: 10.1002/2211-5463.12948 - Maruyama K, Kidoya H, Takemura N, Sugisawa E, Takeuchi O, Kondo T, Eid MMA, Tanaka H, Martino MM, Takakura N, Takayama Y, Akira S, Vandenbon A, Kumagai Y
Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
Cell Rep. 32: 107906 (2020) doi:10.1016/j.celrep.2020.107906 - Sugisawa E, Takayama Y, Takemura N, Kondo T, Hatakeyama S, Kumagai Y, Sunagawa M, Tominaga M, Maruyama K
RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis
Cell. 8674: 30754-6 (2020) doi:10.1016/j.cell.2020.06.022 - Takayama Y, Kushige H, Akagi Y, Suzuki Y, Kumagai Y, Kida YS*
Selective induction of Human Autonomic neurons enables precise control of cardiomyocyte Beating
Sci Rep.10: 9464 (2020) doi:10.1038/s41598-020-66303-3 - Mori N, Akagi Y, Imai Y, Takayama Y, Kida YS*
Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue
Sci Rep. 10: 5646 (2020) doi: 10.1038/s41598-020-62286-3 - Baba T, Takagi T, Sumaru K, Kanamori T
Effect of the fluorination degree of partially fluorinated octyl-phosphocholine surfactants on their interfacial properties and interactions with purple membrane as a membrane protein model
Chemistry And Physics Lipids 227 0: 0 (2020) doi: 10.1016/j.chemphyslip.2020.104870 - Marx U, Akabane T, Andersson TB, Baker E, Beilmann M, Beken S, Brendler-Schwaab S, Cirit M, David R, Dehne EM, Durieux I, Ewart L, Fitzpatrick SC, Frey O, Fuchs F, Griffith LG, Hamilton GA, Hartung T, Hoeng J, Hogberg H, Hughes DJ, Ingber DE, Iskandar A, Kanamori T, Kojima H, Kuehnl J, Leist M, Li B, Loskill P, Mendrick DL, Neumann T, Pallocca G, Rusyn I, Smirnova L, Steger-Hartmann T, Tagle DA, Tonevitsky A, Tsyb S, Trapecar M, Van de Water B, Van den Eijnden-van Raaij J, Vulto P, Watanabe K, Wolf A, Zhou X, Roth A
Biology-inspired microphysiological systems to advance patient benefit and animal welfare in drug development
Altex. 12: 0 (2020) doi: 10.14573/altex.2001241 - Komatsu H, Sakai K, Baba T, Takagi T, Kanamori T
Aggregation behavior of short-chained archaeal phospholipid analogs: Contribution methyl branches to lipid hydrophobicity and membrane formability
Colloid And Interface Science Communications 129: 3827-3832 (2019) doi: 10.1016/j.colcom.2019.100200 - Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center
J. Physical Chemistry A 123: 3985-3991 (2019) doi: 10.1021/acs.jpca.8b11613 - Ishida S, Kanamori T
Microphysiological system as a promising technology for drug assay
Nihon Yakurigaku Zasshi. 154: 345-351 (2019) doi: 10.1254/fpj.154.345 - Kise R, Fukumi A, Shioya N, Shimoaka T, Sonoyama M, Amii H, , Takagi T, Kanamori T, Eda K, Hasegawa T
Fluorous Property of a Short Perfluoroalkyl-Containing Compound Realized by Self-Assembled Monolayer Technique on a Silicon Substrate
Bulletin The Chemical Society Japan 92: 785-789 (2019) doi: 10.1246/bcsj.20180356 - Tsukamoto T, Sogo T, Ueyama T, Nakao S, Harada Y, Ihara D, Akagi, Y, Kida YS, Hasegawa K, Nagamune T, Kawahara M*, Kawamura T*
Chimeric G-CSF Receptor-Mediated STAT3 Activation Contributes to Efficient Induction of Cardiomyocytes from Mouse Induced Pluripotent Stem Cells
Biotechnology J. 93: 10 (2019) doi: 10.1002/biot.201900052 - Takahashi H, Yoshino M, Morita K, Takagi T, Yokoyama Y, Kikukawa T, Amii H, Kanamori T, Sonoyama M
Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
Biochim Biophys Acta Biomembr. 1861: 631-642 (2019) doi: 10.1016/j.bbamem.2018.12.015 - Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
Commun Biol. 1: 218 (2018) doi: 10.1038/s42003-018-0222-4 - Mori N, Morimoto Y, Takeuchi S*
Perfusable and stretchable 3D culture system for skin-equivalent
Biofabrication. 11: 011001 (2018) doi: 10.1088/1758-5090/aaed12 - Vandenbon A#, Kumagai Y#, Lin M, Suzuki Y, Nakai K*
Waves of chromatin modifications in mouse dendritic cells in response to LPS stimulation
Genome Biol. 19: 138 (2018) doi: 10.1186/s13059-018-1524-z - Maruyama K*, Takayama Y, Sugisawa E, Yamanoi Y, Yokawa T, Kondo T, Ishibashi KI, Sahoo BR, Takemura N, Mori Y, Kanemaru H, Kumagai Y, Martino MM, Yoshioka Y, Nishijo H, Tanaka H, Sasaki A, Ohno N, Iwakura Y, Moriyama Y, Nomura M, Akira S, Tominaga M*
The ATP Transporter VNUT Mediates Induction of Dectin-1-Triggered Candida Nociception
iScience. 0: 0 (2018) doi: 10.1016/j.isci.2018.08.007 - Wagatsuma A*, Takayama Y, Hoshino T, Shiozuka M, Yamada S, Matsuda R, Mabuchi K
Pharmacological targeting of HSP90 with 17-AAG induces apoptosis of myogenic cells through activation of the intrinsic pathway
Mol Cell Biochem. 163: 170-182 (2018) doi: 10.1007/s11010-017-3250-3 - Sumaru K, Takagi T, Morishita K, Satoh T, Kanamori T
Fabrication of pocket-like hydrogel microstructures through photolithography
St Matter 14: 5710-5714 (2018) doi: 10.1039/c8sm00865e - Sumaru K, Takagi T, Morishita K, Kanamori T
Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
Biomacromolecules. 19: 2913-2922 (2018) doi: 10.1021/acs.biomac.8b00470 - Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
Commun Biol. 0: 0 (2018) doi: 10.1038/s42003-018-0222-4 - Sumaru K, Takagi T, Satoh T, Kanamori T
Photo- and Thermoresponsive Dehydration of Spiropyran-Functionalized Polymer Regulated by Molecular Recognition
Macromol Rapid Commun. 39: 0 (2018) doi: 10.1002/marc.201700234 - Ito N, Katoh K, Kushige H, Saito Y, Umemoto T, Matsuzaki Y, Kiyonari H, Kobayashi D, Soga M, Era T, Araki N, Furuta Y, Suda T, Kida Y, Ohta K*
Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency
Sci Rep. 8: 1634 (2018) doi: 10.1038/s41598-018-20057-1 - Ueyama T, Nakao S, Tsukamoto T, Ihara D, Harada Y, Akagi Y, Nakagawa S, Kida YS, Sogo T and Kawamura T*
PTEN/Akt Axis is Involved in Somatic Cell Reprogramming to Mouse iPS Cells
Biomed J Sci & Tech. Res. 11: 5 (2018) DOI: 10.26717/BJSTR.2018.11.002162 - Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
Study of Perfluoroalkyl Chain-Specific Band Shift in Infrared Spectra on the Chain Length
J. Physical Chemistry A 121: 8425-8431 (2017) doi: 10.1021/acs.jpca.7b08940 - Sumaru K, Morishita K, Takagi T, Satoh T, Kanamori T
Sectioning of cultured cell monolayer using photo-acid-generating substrate and micro-patterned light projection
European Polymer J. 121: 16964-16969 (2017) doi: 10.1016/j.eurpolymj.2017.02.021 - Watanabe Y, Miyasaka KY, Kubo A, Kida YS, Nakagawa O, Hirate Y, Sasaki H, Ogura T*
Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm
Sci Rep. 0: 0 (2017) doi: 10.1038/srep46135 - Kondo Y, Yada Y, Haga T, Takayama Y, Isomura T, Jimbo Y, Fukayama O, Hoshino T*, Mabuchi K
Temporal relation between neural activity and neurite pruning on a numerical model and a microchannel device with micro electrode array
Biochem Biophys Res Commun. 486: 539-544 (2017) doi: 10.1016/j.bbrc.2017.03.082 - Takayama Y#, Wakabayashi T#, Kushige H, Saito Y, Shibuya Y, Shibata S, Akamatsu W, Okano H, Kida YS*
Brief Exposure to Small Molecules Allows Induction of Mouse Embryonic Fibroblasts Into Neural Crest-Like Precursors
FEBS Lett. 591: 590-602 (2017) doi: 10.1002/1873-3468.12572