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研究課題

例えば、食品は自律神経系である迷走神経を介して脳機能や全身の生理機能をコントロールしています。このような自律神経機能は交感神経と副交感神経のバランスに依存してます。動物愛護の観点から、身体の生理機能を生体外で再現する動物実験代替法の開発ニーズが高まっています。我々はヒト幹細胞からの交感神経と副交感神経を誘導する方法を開発しました。本研究課題では脳腸相関などの生体の迷走神経路を模倣する動物実験代替法とその評価法を開発します。

研究課題

難治性固形がんである膵癌では、がん間質の線維化が障壁となり、治療薬が腫瘍の深部にまで浸透しないという間質バリアが治療の課題です。我々は、がん関連線維芽細胞や免疫細胞で構成されるがん微小環境を研究するための実験系構築を進めてきました。本研究課題では、がんの基盤研究や治療法開発、診断に向けた細胞誘導法や3次元組織構築法を開発します。

メンバー

業績リスト

• Kumagai, Y; Saito, Y; Kida, YS.
  A multiomics atlas of brown adipose tissue development over time.
  ENDOCRINOLOGY. 2023 Apr 21:bqad064. doi: 10.1210/endocr/bqad064
• Sogo, T; Nakao, S; Tsukamoto, T; Ueyama, T; Harada, Y; Ihara, D; Ishida, T; Nakahara, M; Hasegawa, K; Akagi, Y; Kida, YS; Nakagawa, O; Nagamune, T; Kawahara, M; Kawamura, T.
  Canonical Wnt signaling activation by chimeric antigen receptors for efficient cardiac differentiation from mouse embryonic stem cells.
  INFLAMM REGEN. 2023 Feb 10;43(1):11. doi: 10.1186/s41232-023-00258-6
• Nohira, N; Shinji, S; Nakamura, S; Nihashi, Y; Shimosato, T; Takaya, T.
  Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells.
  BIOMEDICINES. 2022 Oct 25;10(11):2691. doi: 10.3390/biomedicines10112691
• Imai, Y;Mori, N; Nihashi, Y; Kumagai, Y; Shibuya, Y; Oshima, J; Sasaki, M; Sasaki, K; Aihara, Y; Sekido, M; Kida, YS.
  Therapeutic Potential of Adipose Stem Cell-Derived Conditioned Medium on Scar Contraction Model.
  BIOMEDICINES. 2022 Sep 24;10(10):2388. doi: 10.3390/biomedicines10102388
• Sugisawa, E; Kondo, T; Kumagai, Y; Kato, H; Takayama, Y; Isohashi, K; Shimosegawa, E; Takemura, N; Hayashi, Y; Sasaki, T; Martino, MM; Tominaga, M; Maruyama, K.
  Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
  CELL REP. 2022 Mar 8;38(10):110462. doi: 10.1016/j.celrep.2022.110462
• Miyazaki, Y; Mori, N; Akagi, Y; Oda, T; Kida, YS.
  Potential Metabolite Markers for Pancreatic Cancer Identified by Metabolomic Analysis of Induced Cancer-Associated Fibroblasts.
  CANCERS. 2022 Mar 8;14(6):1375. doi: 10.3390/cancers14061375
• Akagi, Y; Mori, N; Kawamura, T; Takayama, Y; Kida, YS.
  Non-invasive cell classification using the Paint Raman Express Spectroscopy System (PRESS)
  SCI REP. 2021 Apr 23;11(1):8818. doi: 10.1038/s41598-021-88056-3
• Mori, N; Kida, YS.
  Applicability of Artificial Vascularized Liver Tissue to Proteomic Analysis.
  MICROMACHINES (Basel). 2021 Apr 11;12(4):418. doi: 10.3390/mi12040418
• Miyazaki Y, Oda T, Inagaki Y, Kushige H, Saito Y, Mori N, Takayama Y, Kumagai Y, Mitsuyama T, Kida YS
  Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.
  SCI REP. 2021 Feb 25;11(1):4690. doi: 10.1038/s41598-021-84058-3
• Kumagai Y, Hirasawa T, Wachi M
  Requirement of the LtsA Protein for Formation of the Mycolic Acid-Containing Layer on the Cell Surface of Corynebacterium glutamicum.
  MICROORGANISMS. 2021 Feb 16;9(2):409. doi: 10.3390/microorganisms9020409
• Baba T, Takagi T, Sumaru K, Kanamori T, Dewa T, Nango M
  Membrane properties of ether-type phosphatidylcholine bearing partially fluorinated C(18)-monoacetylenic chains and their applicability to membrane protein reconstitution matrices
  Colloids Surf B Biointerfaces. 2020 Nov 13:111459. doi: 10.1016/j.colsurfb.2020.111459
• Takayama Y, Akagi Y, Shibuya Y, Kida YS
  Exposure to small molecule cocktails allows induction of neural crest lineage cells from human adipose-derived mesenchymal stem cells
  PLoS One. ;15(10):e0241125. (2020)doi: 10.1371/journal.pone.0241125
• Miyazaki Y, Oda T, Mori N, Kida YS
  Adipose-derived mesenchymal stem cells differentiate into pancreatic cancer-associated fibroblasts in vitro
  FEBS Open Bio. 2020 Sep 15. (2020) doi: 10.1002/2211-5463.12976
• Mori N, Kida YS
  Expression of genes involved in drug metabolism differs between perfusable 3D liver tissue and conventional 2D-cultured hepatocellular carcinoma cells
  FEBS Open Bio. 0 (2020) Aug 14. doi: 10.1002/2211-5463.12948
• Maruyama K, Kidoya H, Takemura N, Sugisawa E, Takeuchi O, Kondo T, Eid MMA, Tanaka H, Martino MM, Takakura N, Takayama Y, Akira S, Vandenbon A, Kumagai Y
  Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
  Cell Rep. 32: 107906 (2020) doi:10.1016/j.celrep.2020.107906
• Sugisawa E, Takayama Y, Takemura N, Kondo T, Hatakeyama S, Kumagai Y, Sunagawa M, Tominaga M, Maruyama K
  RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis
  Cell. 8674: 30754-6 (2020) doi:10.1016/j.cell.2020.06.022
• Takayama Y, Kushige H, Akagi Y, Suzuki Y, Kumagai Y, Kida YS^*
  Selective induction of Human Autonomic neurons enables precise control of cardiomyocyte Beating
  Sci Rep.10: 9464 (2020) doi:10.1038/s41598-020-66303-3
• Mori N, Akagi Y, Imai Y, Takayama Y, Kida YS^*
  Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue
  Sci Rep. 10: 5646 (2020) doi: 10.1038/s41598-020-62286-3
• Baba T, Takagi T, Sumaru K, Kanamori T
  Effect of the fluorination degree of partially fluorinated octyl-phosphocholine surfactants on their interfacial properties and interactions with purple membrane as a membrane protein model
  Chemistry And Physics Lipids 227 0: 0 (2020) doi: 10.1016/j.chemphyslip.2020.104870
• Marx U, Akabane T, Andersson TB, Baker E, Beilmann M, Beken S, Brendler-Schwaab S, Cirit M, David R, Dehne EM, Durieux I, Ewart L, Fitzpatrick SC, Frey O, Fuchs F, Griffith LG, Hamilton GA, Hartung T, Hoeng J, Hogberg H, Hughes DJ, Ingber DE, Iskandar A, Kanamori T, Kojima H, Kuehnl J, Leist M, Li B, Loskill P, Mendrick DL, Neumann T, Pallocca G, Rusyn I, Smirnova L, Steger-Hartmann T, Tagle DA, Tonevitsky A, Tsyb S, Trapecar M, Van de Water B, Van den Eijnden-van Raaij J, Vulto P, Watanabe K, Wolf A, Zhou X, Roth A
  Biology-inspired microphysiological systems to advance patient benefit and animal welfare in drug development
  Altex. 12: 0 (2020) doi: 10.14573/altex.2001241
• Komatsu H, Sakai K, Baba T, Takagi T, Kanamori T
  Aggregation behavior of short-chained archaeal phospholipid analogs: Contribution methyl branches to lipid hydrophobicity and membrane formability
  Colloid And Interface Science Communications 129: 3827-3832 (2019) doi: 10.1016/j.colcom.2019.100200
• Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
  Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center
  J. Physical Chemistry A 123: 3985-3991 (2019) doi: 10.1021/acs.jpca.8b11613
• Ishida S, Kanamori T
  Microphysiological system as a promising technology for drug assay
  Nihon Yakurigaku Zasshi. 154: 345-351 (2019) doi: 10.1254/fpj.154.345
• Kise R, Fukumi A, Shioya N, Shimoaka T, Sonoyama M, Amii H, , Takagi T, Kanamori T, Eda K, Hasegawa T
  Fluorous Property of a Short Perfluoroalkyl-Containing Compound Realized by Self-Assembled Monolayer Technique on a Silicon Substrate
  Bulletin The Chemical Society Japan 92: 785-789 (2019) doi: 10.1246/bcsj.20180356
• Tsukamoto T, Sogo T, Ueyama T, Nakao S, Harada Y, Ihara D, Akagi, Y, Kida YS, Hasegawa K, Nagamune T, Kawahara M*, Kawamura T*
  Chimeric G-CSF Receptor-Mediated STAT3 Activation Contributes to Efficient Induction of Cardiomyocytes from Mouse Induced Pluripotent Stem Cells
  Biotechnology J. 93: 10 (2019) doi: 10.1002/biot.201900052
• Takahashi H, Yoshino M, Morita K, Takagi T, Yokoyama Y, Kikukawa T, Amii H, Kanamori T, Sonoyama M
  Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
  Biochim Biophys Acta Biomembr. 1861: 631-642 (2019) doi: 10.1016/j.bbamem.2018.12.015
• Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
  Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
  Commun Biol. 1: 218 (2018) doi: 10.1038/s42003-018-0222-4
• Mori N, Morimoto Y, Takeuchi S*
  Perfusable and stretchable 3D culture system for skin-equivalent
  Biofabrication. 11: 011001 (2018) doi: 10.1088/1758-5090/aaed12
• Vandenbon A^#, Kumagai Y^#, Lin M, Suzuki Y, Nakai K^*
  Waves of chromatin modifications in mouse dendritic cells in response to LPS stimulation
  Genome Biol. 19: 138 (2018) doi: 10.1186/s13059-018-1524-z
• Maruyama K^*, Takayama Y, Sugisawa E, Yamanoi Y, Yokawa T, Kondo T, Ishibashi KI, Sahoo BR, Takemura N, Mori Y, Kanemaru H, Kumagai Y, Martino MM, Yoshioka Y, Nishijo H, Tanaka H, Sasaki A, Ohno N, Iwakura Y, Moriyama Y, Nomura M, Akira S, Tominaga M*
  The ATP Transporter VNUT Mediates Induction of Dectin-1-Triggered Candida Nociception
  iScience. 0: 0 (2018) doi: 10.1016/j.isci.2018.08.007
• Wagatsuma A^*, Takayama Y, Hoshino T, Shiozuka M, Yamada S, Matsuda R, Mabuchi K
  Pharmacological targeting of HSP90 with 17-AAG induces apoptosis of myogenic cells through activation of the intrinsic pathway
  Mol Cell Biochem. 163: 170-182 (2018) doi: 10.1007/s11010-017-3250-3
• Sumaru K, Takagi T, Morishita K, Satoh T, Kanamori T
  Fabrication of pocket-like hydrogel microstructures through photolithography
  St Matter 14: 5710-5714 (2018) doi: 10.1039/c8sm00865e
• Sumaru K, Takagi T, Morishita K, Kanamori T
  Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
  Biomacromolecules. 19: 2913-2922 (2018) doi: 10.1021/acs.biomac.8b00470
• Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
  Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
  Commun Biol. 0: 0 (2018) doi: 10.1038/s42003-018-0222-4
• Sumaru K, Takagi T, Satoh T, Kanamori T
  Photo- and Thermoresponsive Dehydration of Spiropyran-Functionalized Polymer Regulated by Molecular Recognition
  Macromol Rapid Commun. 39: 0 (2018) doi: 10.1002/marc.201700234
• Ito N, Katoh K, Kushige H, Saito Y, Umemoto T, Matsuzaki Y, Kiyonari H, Kobayashi D, Soga M, Era T, Araki N, Furuta Y, Suda T, Kida Y, Ohta K^*
  Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency
  Sci Rep. 8: 1634 (2018) doi: 10.1038/s41598-018-20057-1
• Ueyama T, Nakao S, Tsukamoto T, Ihara D, Harada Y, Akagi Y, Nakagawa S, Kida YS, Sogo T and Kawamura T^*
  PTEN/Akt Axis is Involved in Somatic Cell Reprogramming to Mouse iPS Cells
  Biomed J Sci & Tech. Res. 11: 5 (2018) DOI: 10.26717/BJSTR.2018.11.002162
• Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
  Study of Perfluoroalkyl Chain-Specific Band Shift in Infrared Spectra on the Chain Length
  J. Physical Chemistry A 121: 8425-8431 (2017) doi: 10.1021/acs.jpca.7b08940
• Sumaru K, Morishita K, Takagi T, Satoh T, Kanamori T
  Sectioning of cultured cell monolayer using photo-acid-generating substrate and micro-patterned light projection
  European Polymer J. 121: 16964-16969 (2017) doi: 10.1016/j.eurpolymj.2017.02.021
• Watanabe Y, Miyasaka KY, Kubo A, Kida YS, Nakagawa O, Hirate Y, Sasaki H, Ogura T^*
  Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm
  Sci Rep. 0: 0 (2017) doi: 10.1038/srep46135
• Kondo Y, Yada Y, Haga T, Takayama Y, Isomura T, Jimbo Y, Fukayama O, Hoshino T^*, Mabuchi K
  Temporal relation between neural activity and neurite pruning on a numerical model and a microchannel device with micro electrode array
  Biochem Biophys Res Commun. 486: 539-544 (2017) doi: 10.1016/j.bbrc.2017.03.082
• Takayama Y^#, Wakabayashi T#, Kushige H, Saito Y, Shibuya Y, Shibata S, Akamatsu W, Okano H, Kida YS^*
  Brief Exposure to Small Molecules Allows Induction of Mouse Embryonic Fibroblasts Into Neural Crest-Like Precursors
  FEBS Lett. 591: 590-602 (2017) doi: 10.1002/1873-3468.12572