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分子機能応用研究グループ
研究概要
培養細胞が盛んに利活用される昨今、これまで以上にこれら細胞を精密に制御する技術へのニーズが高まっています。また、生体機能を司るバイオ分子として、糖タンパクが注目されています。
私たちは、光応答性ポリマーやDNA・RNAなどの高分子、フッ素化脂質で意のままに細胞を操作する、そして糖タンパクを生産・機能化・分析するためのたくさんの要素技術を確立し、その応用・実用化を進めています。
研究課題
研究課題1:細胞を操る分子技術
研究担当者:須丸 公雄・高木 俊之・馬場 照彦・佐野 将之
新開発の光応答ポリマーを用いて培養細胞を思いのままに配列・選別する技術、持続発現型RNAベクターを用いて細胞のリプログラミング・機能発現解析を行う技術、細胞の機能化への展開が期待されるフッ素化脂質、膜タンパク操作技術を開発しました。
研究課題
研究課題2:糖タンパク質製造分析技術
研究担当者:亀山昭彦・浅田眞弘・横尾岳彦・千葉靖典
新しい膜電気泳動法により、巨大糖タンパクの精密分析を実現しました。また、特別な酵母で有用な糖タンパク質を大量かつ安価に生産する技術、タンパク質に糖鎖を付加し、生体内での安定性や標的選択性を向上させる技術を開発しました。
研究課題
研究課題3:サブテラヘルツ波を利用し、水和と生物分子機能の関係を探る
研究担当者:今清水 正彦
生物は、熱運動に打ち勝てないほど弱い、生体高分子と水の相互作用を集団的に作用させ、DNA分子情報のコピーのような極めて正確な分子機能を作り上げています。その興味深い機構には、本質的な分子機能応用のヒントが隠されていますが、多くは謎のままです。私達は、生体高分子と水の集団相互作用のタイムスケールと周期が対応するサブテラヘルツ(sub-THz)波を利用し、分光学的・生化学的な観測と組み合わせて、この謎に挑戦しています。
研究課題
研究課題4:細胞を操る分子技術：細胞膜を修飾するバイオマテリアル
研究担当者:馬場 照彦・高木 俊之・寺村 裕治
生体適合性の高い両親媒性高分子を用いて、細胞や臓器を修飾することで、細胞操作や細胞保護が可能になることが分かってきました。この技術を用いて、細胞だけでなく、リポソームやエクソソームの表面加工へと応用展開しています。
メンバー
| 名前 | 役職 | 主な研究テーマ |
|---|---|---|
| 須丸 公雄 SUMARU Kimio | グループ長 | ・光応答性培養基材を用いた細胞プロセシング ・新規光応答ポリマー材料の開発と応用 ・光異性化分子材料のバイオ応用 |
| 亀山 昭彦 KAMEYAMA Akihiko | 上級主任研究員 | ・グライコミクス方法論の開発 ・分子マトリックス電気泳動法の開発と応用 ・腫瘍および老化や認知症におけるムチンの機能解明 |
| 寺村 裕治 TERAMURA Yuji | 上級主任研究員 | ・細胞と臓器移植に役立つバイオマテリアルの研究・開発 ・細胞並びに血管内皮表面を修飾できる高分子材料の研究・開発 ・エクソソーム・細胞をin vitroとin vivoで操作できる材料の研究・開発 |
| 馬場 照彦 BABA Teruhiko | 主任研究員 | ・機能性タンパク質を支える安定な人工脂質の特性評価技術 ・機能性タンパク質の安定化技術 ・人工脂質-機能性タンパク質複合系の生物工学的応用 |
| 浅田 眞弘 ASADA Masahiro | 主任研究員 | ・複合糖質の構造と機能の解析 ・iPS細胞の利用技術の開発 ・生理活性物質の探索・機能解析・産業応用 |
| 横尾 岳彦 YOKO－O Takehiko | 主任研究員 | ・酵母による異種タンパク質生産 ・GPIアンカーの生合成機構 ・糖転移酵素の局在化機構 |
| 高木 俊之 TAKAGI Toshiyuki | 主任研究員 | ・膜タンパク質－脂質膜複合型バイオセンサの開発 ・部分フッ素化および擬環状型脂質の開発 ・光応答性材料の開発 |
| 佐野 将之 SANO Masayuki | 主任研究員 | ・欠損持続発現型RNAベクターの開発 ・幹細胞制御技術の開発 ・遺伝子発現制御技術の開発 |
| 今清水 正彦 IMASHIMIZU Masahiko | 主任研究員 | ・熱運動を利用する生物反応を理解するための摂動手段の獲得 ・THz光による水・生体高分子の運動変化の直接観測 ・THz光の非熱的効果の検証 |
業績リスト
- Rangasami, VK; Asawa, K; Teramura, Y; Le, Blanc K; Nilsson, B; Hilborn, J; Varghese, OP; Oommen, OP.
Biomimetic polyelectrolyte coating of stem cells suppresses thrombotic activation and enhances its survival and function.
BIOMATER ADV. 2023 Feb 8;147:213331. doi: 10.1016/j.bioadv.2023.213331 - Huang, T; Sato, Y; Kuramochi, A; Ohba, Y; Sano, M; Miyagishi, M; Tateno, H; Wadhwa, R; Kawasaki, K; Uchida, T; Ekdahl, KN; Nilsson, B; Chung, UI; Teramura, Y.
Surface modulation of extracellular vesicles with cell-penetrating peptide-conjugated lipids for improvement of intracellular delivery to endothelial cells.
REGEN THER. 2023 Jan 11;22:90-98. doi: 10.1016/j.reth.2022.12.007 - Tsuda, M; Nakatani, Y; Baba, S; Tanaka, I; Ichikawa, K; Nonaka, K; Ito, R; Yoko-O, T; Chiba, Y.
Selection of the methylotrophic yeast Ogataea minuta as a high-producing host for heterologous protein expression.
J BIOSCI BIOENG. 2023 Jan 24:S1389-1723(22)00375-9. doi: 10.1016/j.jbiosc.2022.12.006 - Suzuki, H; Adler, A; Huang, T; Kuramochi, A; Ohba, Y; Sato, Y; Nakamura, N; Manivel, VA; Ekdahl, KN; Nilsson, B; Ishihara, K; Teramura, Y.
Impact of spontaneous liposome modification with phospholipid polymer-lipid conjugates on protein interactions.
SCI TECHNOL ADV MATER. 2022 Dec 8;23(1):845-857. doi: 10.1080/14686996.2022.2146466 - Ekdahl, KN; Fromell, K; Mannes, M; Grinnemo, KH; Huber-Lang, M;Teramura, Y; Nilsson, B.
Therapeutic regulation of complement activation in extracorporeal circuits and intravascular treatments with special reference to the alternative pathway amplification loop.
IMMUNOL REV. 2022 Oct 18. doi: 10.1111/imr.13148 - Sano, M; Morishita, K; Onizawa, Y; Takagi, T; Sumaru, K.
Rapid and Highly Sensitive Method for Evaluating Surface Coatings against an Enveloped RNA Virus.
ACS APPL BIO MATER. 2022 Oct 14. doi: 10.1021/acsabm.2c00613 - Mellul, M; Lahav, S; Imashimizu, M; Tokunaga, Y; Lukatsky, DB; Ram, O.
Repetitive DNA symmetry elements negatively regulate gene expression in embryonic stem cells.
BIOPHYS J. 2022 Jul 8:S0006-3495(22)00557-4. doi: 10.1016/j.bpj.2022.07.011 - Omoto, T; Yimiti, D; Sanada, Y; Toriyama, M; Ding, C; Hayashi, Y; Ikuta, Y; Nakasa, T; Ishikawa, M; Sano, M; Lee, M; Akimoto, T; Shukunami, C; Miyaki, S; Adachi, N.
Tendon-Specific Dicer Deficient Mice Exhibit Hypoplastic Tendon Through the Downregulation of Tendon-Related Genes and MicroRNAs.
FRONT CELL DEV BIOL. 2022 Jun 14;10:898428. doi: 10.3389/fcell.2022.898428 - Adler, A; Manivel, VA; Fromell, K; Teramura, Y; Ekdahl, KN; Nilsson, B.
A Robust Method to Store Complement C3 With Superior Ability to Maintain the Native Structure and Function of the Protein.
FRONT IMMUNOL. 2022 May 3;13:891994. doi: 10.3389/fimmu.2022.891994 - Nakagawa, Y; Hishida, T; Hatano, E; Sumaru, K; Morishita, K; Morimoto, M; Yokojima, S; Nakamura, S; Uchida, K.
Photoinduced cytotoxicity of photochromic symmetric diarylethene derivatives: the relation of structure and cytotoxicity.
ORGANIC & BIOMOLECULAR CHEMISTRY. 2022 Apr 13;20(15):3211-3217. doi: 10.1039/d2ob00224h - Sogabe, M; Kojima, S; Kaya, T; Tomioka, A; Kaji, H; Sato, T; Chiba, Y; Shimizu, A; Tanaka, N; Suzuki, N; Hayashi, I; Mikami, M; Togayachi, A; Narimatsu, H.
Sensitive New Assay System for Serum Wisteria floribunda Agglutinin-Reactive Ceruloplasmin That Distinguishes Ovarian Clear Cell Carcinoma from Endometrioma.
ANALYTICAL CHEMISTRY; doi:10.1021/acs.analchem.1c04302 - Goel, I; Noiri, M; Yamauchi, Y; Kato, K; Chung, UI; Teramura, Y.
Enhancement of intercellular interaction between iPSC-derived neural progenitor cells and activated endothelial cells using cell surface modification with functional oligopeptides.
BIOMATER SCI. 2022 Jan 11. doi: 10.1039/d1bm01503f - Matsuzawa, T; Watanabe, M; Nakamichi, Y; Kameyama, A; Kojima, N; Yaoi, K.
Characterization of an extracellular α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae.
APPL MICROBIOL BIOTECHNOL. 2021 Dec 31. doi: 10.1007/s00253-021-11744-7 - Hurd, MD; Goel, I; Sakai, Y; Teramura, Y.
Current status of ischemic stroke treatment: From thrombolysis to potential regenerative medicine.
REGENERATIVE THERAPY 18 408-417; doi:10.1016/j.reth.2021.09.009 DEC - Adler, A; Inoue, Y; Ekdahl, KN; Baba, T; Ishihara, K; Nilsson, B; Teramura, Y.
Effect of liposome surface modification with water-soluble phospholipid polymer chain-conjugated lipids on interaction with human plasma proteins.
J MATER CHEM B. 2021 Oct 7. doi: 10.1039/d1tb01485d - Sano, M; Morishita, K; Oikawa, S; Akimoto, T; Sumaru, K; Kato, Y.
Live-cell imaging of microRNA expression with post-transcriptional feedback control.
MOL THER NUCLEIC ACIDS. 2021 Aug 26;26:547-556. doi: 10.1016/j.omtn.2021.08.018 - Yoko-O, T; Komatsuzaki, A; Yoshihara, E; Zhao, S; Umemura, M; Gao, XD; Chiba, Y.
Regulation of alcohol oxidase gene expression in methylotrophic yeast Ogataea minuta.
J BIOSCI BIOENG. 2021 Aug 27:S1389-1723(21)00204-8. doi: 10.1016/j.jbiosc.2021.08.001 - Isaka, E; Sugiura, T; Hashimoto, K; Kikuta, K; Anazawa, U; Nomura, T; Kameyama, A.
Characterization of tumor-associated MUC1 and its glycans expressed in mucoepidermoid carcinoma.
ONCOLOGY LETTERS 22 (4);doi: 10.3892/ol.2021.12963 OCT - Sato, Y; Asawa, K; Huang, T; Noiri, M; Nakamura, N; Ekdahl, KN; Nilsson, B; Ishihara, K; Teramura, Y.
Induction of Spontaneous Liposome Adsorption by Exogenous Surface Modification with Cell-Penetrating Peptide-Conjugated Lipids.
LANGMUIR. 2021 Aug 3. doi: 10.1021/acs.langmuir.1c01072 - Adler, A; Inoue, Y; Sato, Y; Ishihara, K; Ekdahl, KN; Nilsson, B; Teramura, Y.
Synthesis of poly(2-methacryloyloxyethyl phosphorylcholine)-conjugated lipids and their characterization and surface properties of modified liposomes for protein interactions.
BIOMATER SCI. 2021 Jul 20. doi: 10.1039/d1bm00570g - Hashimoto, M; Murai, Y; Morita, K; Kikukawa, T; Takagi, T; Takahashi, H; Yokoyama, Y; Amii, H; Sonoyama, M.
Comparison of functionality and structural stability of bacteriorhodopsin reconstituted in partially fluorinated dimyristoylphosphatidylcholine liposomes with different perfluoroalkyl chain lengths.
BIOCHIM BIOPHYS ACTA BIOMEMBR. 2021 Jun 25;1863(10):183686. doi: 10.1016/j.bbamem.2021.183686 - Tsuchida N, Takagi T, Takahashi H, Yoshihara T, Tobita S, Sonoyama M
Membrane properties of amacrocyclic tetraether bisphosphatidylcholine lipid: Effect of a single membrane-spanning polymethylene cross-linkage between two head groups of ditetradecylphosphatidylcholine membrane.
BIOCHIM BIOPHYS ACTA BIOMEMBR. 2021 Feb 4:183569. doi: 10.1016/j.bbamem.2021.183569 - Kameyama A, Nishijima R, Yamakoshi K
Bmi-1 regulates mucin levels and mucin O-glycosylation in the submandibular gland of mice.
PLOS ONE. 2021 Jan 19;16(1):e0245607. doi: 10.1371/journal.pone.0245607. eCollection - Liu D, Liu G, Li Y, Wang Y, Zheng Y, Sha S, Li W, Kameyama A, Dong W
Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.
J PROTEOMICS. 2021 Jan 6:104098. doi: 10.1016/j.jprot.2020.104098 - Baba T, Takagi T, Sumaru K, Kanamori T, Dewa T, Nango M
Membrane properties of ether-type phosphatidylcholine bearing partially fluorinated C(18)-monoacetylenic chains and their applicability to membrane protein reconstitution matrices
Colloids Surf B Biointerfaces. 2020 Nov 13:111459. doi: 10.1016/j.colsurfb.2020.111459 - Kameyama A, Thet Tin WW, Nishijima R, Yamakoshi K
Alteration of mucins in the submandibular gland during aging in mice.
ARCH ORAL BIOL.121:104967 (2020) doi: 10.1016/j.archoralbio.2020.104967 - Matsuzawa T, Kameyama A, Nakamichi Y, Yaoi K
Identification and characterization of two xyloglucan-specific endo-1,4-glucanases in Aspergillus oryzae.
Appl Microbiol Biotechnol. 2020 Sep 10. (2020) doi: 10.1007/s00253-020-10883-7 - Abe T, Kameyama A, Natsuka S, Suzuki N
Sequential modifications of glycans by linkage-specific alkylamidation of sialic acids and permethylation.
Anal Biochem. 606:113861. (2020) doi: 10.1016/j.ab.2020.113861 - Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
ROYAL SOCIETY OPEN SCIENCE 7 (7); (2020) doi: 10.1098/rsos.200027 - Akintayo A, Mayoral J, Asada M, Tang J, Sundaram S, Stanley P
Point Mutations that Inactivate MGAT4D-L, an Inhibitor of MGAT1 and Complex N-Glycan Synthesis
J Biol Chem. :jbc.RA120.014784. (2020) doi: 10.1074/jbc.RA120.014784 - Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T
Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
Royal Society Open Science 7 7: 0 (2020) doi:10.1098/rsos.200027 - Watanabe U,Sugiura S, Kakehata M, Yanagawa F, Takagi T, Sumaru K, Satoh T, Tamura M, Hosokawa Y, Torizuka K, Kanamori T
Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering
Micromachines 11: 0 (2020) doi:10.3390/mi11070679 - Huang GL, Li Z, Li YQ, Liu G, Sun SJ, Gu JG, Kameyama A, Li WZ, Dong WJ
Loss of core fucosylation in both ST6GAL1 and its substrate enhances glycoprotein sialylation in mice
Biochem. J. 477 477: 1179-1201 (2020) doi: 10.1042/BCJ20190789 - Baba T, Takagi T, Sumaru K, Kanamori T
Effect of the fluorination degree of partially fluorinated octyl-phosphocholine surfactants on their interfacial properties and interactions with purple membrane as a membrane protein model
Chemistry And Physics Lipids 227 0: 0 (2020) doi: 10.1016/j.chemphyslip.2020.104870 - Matsuzawa T, Kameyama A, Yaoi K
Identification and characterization of alpha-xylosidase involved in xyloglucan degradation in Aspergillus oryzae
Applied Microbiology And Biotechnology 104 104: 201-210 (2020) doi: 10.1007/s00253-019-10244-z - Sano M, Nakasu A, Ohtaka M, Nakanishi M
A Sendai Virus-Based Cytoplasmic RNA Vector as a Novel Platform for Long-Term
Expression of MicroRNAs
Mol Ther Methods Clin Dev. 15: 371-382 (2019) doi: 10.1016/j.omtm.2019.10.012 - Tsuyukubo A, Sumaru K, Kanamori T
Photolithographic Fabrication of Semi 3-D Microstructures Composed of Flexible Hydrogel Sheet for in Vivo-like Cell Culture System
ACS App Bio Mater. 2: 4129–4133 (2019) doi: 10.1021/acsabm.9b00656, - Komatsu H, Sakai K, Baba T, Takagi T, Kanamori T
Aggregation behavior of short-chained archaeal phospholipid analogs: Contribution methyl branches to lipid hydrophobicity and membrane formability
Colloid And Interface Science Communications 129: 3827-3832 (2019) doi: 10.1016/j.colcom.2019.100200 - Morita K, Horikoshi M, Yanagi T, Takagi T, Takahashi H, Amii H, Hasegawa T, Sonoyama M
Thermotropic Transition Behaviors of Novel Partially Fluorinated Dimyristoylphosphatidylcholines with Different Perfluoroalkyl Chain Lengths
Chemistry Letters 48: 1105-1108 (2019) doi: 10.1246/cl.190389 - Mizuguchi C, Nakagawa M, Namba N, Sakai M, Kurimitsu N, Suzuki A, Fujita K, Horiuchi S, Baba T, Ohgita T, Nishitsuji K, Saito H
Mechanisms of aggregation and fibril formation of the amyloidogenic N-terminal fragment of apolipoprotein A-I
J Biol Chem. 294: 13515-13524 (2019) doi: 10.1074/jbc.RA119.008000 - Matsuzawa T, Watanabe M, Kameda T, Kameyama A, Yaoi K
Cooperation between beta-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae
FEBS JOURNAL 286: 3182-3193 (2019) doi: 10.1111/febs.14848 - Kameyama A
Towards a Novel Platform for Mucin Analysis
Trends In Glycoscience And Glycotechnology 31: 0 (2019) doi: 10.4052/tigg.1918.2SE - Zhao SB, Suda Y, Nakanishi H, Wang N, Yoko-O T, Gao XD, Fujita M
Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network
Biochim Biophys Acta Gen Subj. 1863: 1147-1157 (2019) doi: 10.1016/j.bbagen.2019.04.009 - Kameyama A, Thet Tin WW, Toyoda M, Sakaguchi M
A practical method of liberating O-linked glycans from glycoproteins using hydroxylamine and an organic superbase
Biochem Biophys Res Commun. 513: 186-192 (2019) doi: 10.1016/j.bbrc.2019.03.144 - Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center
J. Physical Chemistry A 123: 3985-3991 (2019) doi: 10.1021/acs.jpca.8b11613 - Yanagi T, Takagi T, Takahashi H, Kikukawa T, Amii H, Sonoyama M
Comparison of two different partially fluorinated phosphatidylcholines with the perfluorobutyl group on thermotropic properties of the bilayer membrane and reconstituted bacteriorhodopsin
Biophys Rev. 6749: 30628-1 (2019) doi: 10.1007/s12551-019-00540-3 - Xu J, Sun HB, Huang GL, Liu G, Li Z, Yang H, Jin LL, Cui XL, , Shi L, Ma TH, Kameyama A, Dong WJ
A fixation method for the optimisation of western blotting
Scientific Reports 116: 8950-8959 (2019) doi: 10.1038/s41598-019-43039-3 - Kise R, Fukumi A, Shioya N, Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Eda K, Hasegawa T
Fluorous Property of a Short Perfluoroalkyl-Containing Compound Realized by Self-Assembled Monolayer Technique on a Silicon Substrate
Bulletin The Chemical Society Japan 92: 785-789 (2019) doi: 10.1246/bcsj.20180356 - Takahashi H, Yoshino M, Morita K, Takagi T, Yokoyama Y, Kikukawa T, Amii H, Kanamori T, Sonoyama M
Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
Biochim Biophys Acta Biomembr. 1861: 631-642 (2019) doi: 10.1016/j.bbamem.2018.12.015 - Isaji T, Im S, Kameyama A, Wang Y, Fukuda T, Gu J
A complex between phosphatidylinositol 4-kinase IIalpha and integrin alpha3beta1 is required for N-glycan sialylation in cancer cells
J Biol Chem. 294: 4425-4436 (2019) doi: 10.1074/jbc.RA118.005208 - Yoko-o T, Komatsuzaki A, Yoshihara E, Umemura M, Chiba Y
Mating type switching, formation of diploids, and sporulation in the methylotrophic yeast Ogataea minuta J. Bioscience And Bioengineering 127: 1-7 (2019) doi: 10.1016/j.jbiosc.2018.07.008 - Kameyama A, Yamakoshi K, Watanabe A
A rapid separation and characterization of mucins from mouse submandibular glands by supported molecular matrix electrophoresis
Biochimica Et Biophysica Acta-Proteins And Proteomics 1867: 76-81 (2019) doi: 10.1016/j.bbapap.2018.05.006 - Iida M, Matsuno YK, Watanabe A, Maruyama M, Kameyama A, Yamakoshi K
A sialo-oligosaccharide-rich mucin-like molecule specifically detected in the submandibular glands of aged mice
Arch Oral Biol. 34: 89-94 (2019) doi: 10.1016/j.archoralbio.2018.10.011 - Matsuzawa T, Kameyama A, Yaoi K
A novel isoprimeverose-producing enzyme from Phaeoacremonium minimum is active with low concentrations of xyloglucan oligosaccharides
FEBS Open Bio. 9: 92-100 (2019) doi: 10.1002/2211-5463.12549 - Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K
Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
Commun Biol. 1: 218 (2018) doi: 10.1038/s42003-018-0222-4 - Shibuta M, Tamura M, Kanie K, Yanagisawa M, Matsui H, Satoh T, , Takagi T, Kanamori T, Sugiura S, Kato R
Imaging cell picker: A morphology-based automated cell separation system on a photodegradable hydrogel culture platform
J. Bioscience And Bioengineering 126: 653-660 (2018) doi: 10.1016/j.jbiosc.2018.05.004 - Xu XX, Li ST, Wang N, Kitajima T, Yoko-O T, Fujita M, Nakanishi H, Gao XD
Structural and functional analysis of Alg1 beta-1,4 mannosyltransferase reveals the physiological importance of its membrane topology
Glycobiology. 28: 741-753 (2018) doi: 10.1093/glycob/cwy060 - Yoko-O T, Umemura M, Komatsuzaki A, Ikeda K, Ichikawa D, Takase K, Kanzawa N, Saito K, Kinoshita T, Taguchi R, Jigami Y
Lipid moiety of glycosylphosphatidylinositol-anchored proteins contributes to the determination of their final destination in yeast
Genes Cells. 23: 880-892 (2018) doi: 10.1111/gtc.12636 - Sumaru K, Takagi T, Morishita K, Satoh T, Kanamori T
Fabrication of pocket-like hydrogel microstructures through photolithography
Soft Matter 14: 5710-5714 (2018) doi: 10.1039/c8sm00865e - Sumaru K, Takagi T, Morishita K, Morishita K, Satoh T, Kanamori T
Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
Biomacromolecules. 19: 2913-2922 (2018) doi: 10.1021/acs.biomac.8b00470 - Mizuguchi C, Nakamura M, Kurimitsu N, Ohgita T, Nishitsuji K, Baba T, Shigenaga A, Shimanouchi T, Okuhira K, Otaka A, Saito H
Effect of Phosphatidylserine and Cholesterol on Membrane-mediated Fibril Formation by the N-terminal Amyloidogenic Fragment of Apolipoprotein A-I
Sci Rep. 8: 5497 (2018) doi: 10.1038/s41598-018-23920-3 - Kameyama A, Dissanayake SK, Thet Tin WW
Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans
PloS One. 13: 0 (2018) doi: 10.1371/journal.pone.0196800 - Xu XX, Komatsuzaki A, Chiba Y, Gao XD, Yoko-O T
PER1, GUP1 and CWH43 of methylotrophic yeast Ogataea minuta are involved in cell wall integrity
Yeast. 35: 225-236 (2018) doi: 10.1002/yea.3285 - Sumaru K, Takagi T, Satoh T, Kanamori T
Photo- and Thermoresponsive Dehydration of Spiropyran-Functionalized Polymer Regulated by Molecular Recognition
Macromol Rapid Commun. 39: 0 (2018) doi: 10.1002/marc.201700234 - Shimoaka T, Sonoyama M, Amii H, Takagi T, Kanamori T, Hasegawa T
Study of Perfluoroalkyl Chain-Specific Band Shift in Infrared Spectra on the Chain Length
J. Physical Chemistry A 121: 8425-8431 (2017) doi: 10.1021/acs.jpca.7b08940 - Sano M, Ohtaka M, Iijima M, Nakasu A, Kato Y, Nakanishi M
Sensitive and long-term monitoring of intracellular microRNAs using a non-integrating cytoplasmic RNA vector
Sci Rep. 7: 12673 (2017) doi: 10.1038/s41598-017-12847-w - Zhou Y, Fukuda T, Hang Q, Hou S, Isaji T, Kameyama A, Gu J
Inhibition of fucosylation by 2-fluorofucose suppresses human liver cancer HepG2 cell proliferation and migration as well as tumor formation
Sci Rep. 7: 11563 (2017) doi: 10.1038/s41598-017-11911-9 - Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M
Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302
Stem Cell Res. 23: 13-19 (2017) doi: 10.1016/j.scr.2017.06.011 - Sumaru K, Morishita K, Takagi T, Satoh T, Kanamori T
Sectioning of cultured cell monolayer using photo-acid-generating substrate and micro-patterned light projection
European Polymer J. 121: 16964-16969 (2017) doi: 10.1016/j.eurpolymj.2017.02.021 - Tamura M, Sugiura S, Takagi T, Satoh T, Sumaru K, Kanamori T, Okada T, Matsui H
Morphology-based optical separation of subpopulations from a heterogeneous murine breast cancer cell line
PloS One. 12: 0 (2017) doi: 10.1371/journal.pone.0179372