生物システム工学特別研究チーム
成果発表

    2017年

  1. Matsui, M., Yokoyama, T., Nemoto, K., Kumagai, T., Terai, G., Tamano, K. et al. Identification of a putative FR901469 biosynthesis gene cluster in fungal sp. No. 11243 and enhancement of the productivity by overexpressing the transcription factor gene frbF. J Biosci and Bioeng. 123, 147-153 (2017).
  2. 2016年

  3. Ye, Y., Minami, A., Igarashi, Y., Izumikawa, M., Umemura, M., Nagano, N. et al. Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi. Angew Chem Int Ed Engl. 55, 8072-8075 (2016).
  4. Kumagai, T., Ishii, T., Terai, G., Umemura, M., Machida, M. & Asai, K. Genome Sequence of Ustilaginoidea virens IPU010, a Rice Pathogenic Fungus Causing False Smut. Genome Announc. 4 (2016).
  5. Yamada, O., Machida, M., Hosoyama, A., Goto, M., Takahashi, T., Futagami, T. et al. Genome sequence of Aspergillus luchuensis NBRC 4314. DNA Research. 23, 507-515 (2016).
  6. Yoshimi, A., Umemura, M., Nagano, N., Koike, H., Machida, M. & Abe, K. Expression of ustR and the Golgi protease KexB are required for ustiloxin B biosynthesis in Aspergillus oryzae. AMB Express. 6, 9 (2016).
  7. Nagano, N., Umemura, M., Izumikawa, M., Kawano, J., Ishii, T., Kikuchi, M. et al. Class of cyclic ribosomal peptide synthetic genes in filamentous fungi. Fungal Genet Biol. 86, 58-70 (2016).
  8. 2015年

  9. Ikegami, T., Inatsugi, T., Kojima, I., Umemura, M., Hagiwara, H., Machida, M. et al. Hybrid De Novo Genome Assembly Using MiSeq and SOLiD Short Read Data. PLoS One. 10, e0126289 (2015).
  10. Tamano, K., Bruno, K.S., Koike, H., Ishii, T., Miura, A., Umemura, M. et al. Increased production of free fatty acids in Aspergillus oryzae by disruption of a predicted acyl-CoA synthetase gene. Appl Microbiol Biotechnol. 99, 3103-3113 (2015).
  11. 2014年

  12. Takeda, I., Umemura, M., Koike, H., Asai, K. & Machida, M. Motif-Independent Prediction of a Secondary Metabolism Gene Cluster Using Comparative Genomics: Application to Sequenced Genomes of Aspergillus and Ten Other Filamentous Fungal Species. DNA Res. 21, 447-457 (2014).
  13. Umemura, M., Nagano, N., Koike, H., Kawano, J., Ishii, T., Miyamura, Y. et al. Characterization of the biosynthetic gene cluster for the ribosomally synthesized cyclic peptide ustiloxin B in Aspergillus flavus. Fungal Genet Biol. 68, 23-30 (2014).
  14. 2013年

  15. Umemura, M., Koike, H., Nagano, N., Ishii, T., Kawano, J., Yamane, N. et al. MIDDAS-M: Motif-Independent De Novo Detection of Secondary Metabolite Gene Clusters through the Integration of Genome Sequencing and Transcriptome Data. PLoS One. 8, e84028 (2013).
  16. Morita, T. et al., Genome Sequence of the Basidiomycetous Yeast Pseudozyma antarctica T-34, a Producer of the Glycolipid Biosurfactants Mannosylerythritol Lipids, Genome Announc, 1, e0006413 (2013).
  17. Umemura, M. et al. Fine De Novo Sequencing of a Fungal Genome Using only SOLiD Short Read Data: Verification on Aspergillus oryzae RIB40. PLoS One 8, e63673 (2013)
  18. Tamano, K. et al. Increased production of fatty acids and triglycerides in Aspergillus oryzae by enhancing expressions of fatty acid synthesis-related genes. Appl Microbiol Biotechnol 97, 269-281 (2013).
  19. Hong, S.B. et al. Aspergillus luchuensis , an industrially important black Aspergillus in East Asia. PLoS One 8, e63769 (2013).
  20. 2012年

  21. Umemura, M. et al. Comparative genome analysis between Aspergillus oryzae strains reveals close relationship between sites of mutation localization and regions of highly divergent genes among Aspergillus species. DNA Res 19, 375-382 (2012).
  22. Nemoto, N. et al. Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on interleukin-6-dependent cell proliferation. Biochem Biophys Res Commun 421, 129-133 (2012).
  23. 2011年

  24. Naimuddin, M. et al. Directed evolution of a three-finger neurotoxin by using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling. Mol Brain 4, 2 (2011).
  25. Marui, J. et al. Kojic acid biosynthesis in Aspergillus oryzae is regulated by a Zn(II)(2)Cys(6) transcriptional activator and induced by kojic acid at the transcriptional level. J Biosci Bioeng 112, 40-43 (2011).
  26. 2010年

  27. Terabayashi, Y. et al. Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae. Fungal Genet Biol 47, 953-961 (2010).
  28. Marui, J. et al. Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering. J Biosci Bioeng 110, 8-11 (2010).
  29. Marui, J. et al. Use of the Aspergillus oryzae actin gene promoter in a novel reporter system for exploring antifungal compounds and their target genes. Appl Microbiol Biotechnol 87, 1829-1840 (2010).
  30. 2009年

  31. Yamaguchi,J. et al. cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions. Nucleic Acids Res. 37, e108 (2009).
  32. Jin,F.J., Takahashi,T., Machida,M. & Koyama,Y. Identification of a basic helix-loop-helix-type transcription regulator gene in Aspergillus oryzae by systematically deleting large chromosomal segments. Appl. Environ. Microbiol. 75, 5943-5951 (2009).
  33. Migita,K. et al. Interleukin-18 promoter polymorphisms and the disease progression of Hepatitis B virus-related liver disease. Transl. Res. 153, 91-96 (2009).
  34. 2008年

  35. Takahashi,T., Jin,F.J., Sunagawa,M., Machida,M. & Koyama,Y. Generation of large chromosomal deletions in koji molds Aspergillus oryzae and Aspergillus sojae via a loop-out recombination. Appl. Environ. Microbiol. 74, 7684-7693 (2008).
  36. Tamano,K. et al. Transcriptional regulation of genes on the non-syntenic blocks of Aspergillus oryzae and its functional relationship to solid-state cultivation. Fungal. Genet. Biol. 45, 139-151 (2008).
  37. Yu,J. et al. Aspergillus flavus genomics as a tool for studying the mechanism of aflatoxin formation. Food Addit. Contam 1-6 (2008).
  38. Machida,M. et al. Genomics of industrial Aspergilli and comparison with toxigenic relatives. Food Addit. Contam Part A Chem. Anal. Control Expo. Risk Assess. 25, 1147-1151 (2008).
  39. Hisada,H., Sano,M., Hata,Y., Abe,Y. & Machida,M. Deletion analysis of the catalase-encoding gene (catB) promoter from Aspergillus oryzae. Biosci. Biotechnol. Biochem. 72, 48-53 (2008).
  40. 2007年

  41. Tamano,K. et al. The beta-1,3-exoglucanase gene exgA (exg1) of Aspergillus oryzae is required to catabolize extracellular glucan, and is induced in growth on a solid surface. Biosci. Biotechnol. Biochem. 71, 926-934 (2007).
  42. Ohashi-Kunihiro,S., Yohda,M., Masaki,H. & Machida,M. A novel vector for positive selection of inserts harboring an open reading frame by translational coupling. Biotechniques 43, 751-2, 754 (2007).
  43. Akao,T. et al. Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions. DNA Res. 14, 47-57 (2007).
  44. Hagiwara,H. et al. Development of an automated SNP analysis method using a paramagnetic beads handling robot. Biotechnol. Bioeng. 98, 420-428 (2007).
  45. Rokas,A. et al. What can comparative genomics tell us about species concepts in the genus Aspergillus? Stud. Mycol. 59, 11-17 (2007).
  46. 2006年

  47. Natsuki,J. & Machida,M. A simple and reliable method for the detection of sialylation in complex/hybrid-type carbohydrate chains of glycoproteins by mixed lectins. Biotechnol. Bioeng. 93, 225-230 (2006).
  48. Hisada,H. et al. Deletion analysis of the superoxide dismutase (sodM) promoter from Aspergillus oryzae. Appl. Microbiol. Biotechnol. 72, 1048-1053 (2006).
  49. Payne,G.A. et al. Whole genome comparison of Aspergillus flavus and A. oryzae. Mdical Mycology 44, 9-11 (2006).
  50. 2005年

  51. Machida,M. et al. Genome sequencing and analysis of Aspergillus oryzae. Nature 438, 1157-1161 (2005).
  52. Nierman,W.C. et al. Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature 438, 1151-1156 (2005).
  53. Galagan J.E. et al. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 438, 1105-1115 (2005).
  54. Maeda,H. et al. Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae. Appl. Microbiol. Biotechnol. 67, 778-788 (2005).
  55. Tamano,K. et al. Improvement of the lectin-antibody enzyme immunoassay of the alphafetoprotein carbohydrate chain for automation with the enzyme immunoassay robot. Biosci. Biotechnol. Biochem. 69, 1616-1619 (2005).
  56. Ueda,H.R. et al. System-level identification of transcriptional circuits underlying mammalian circadian clocks. Nat. Genet. 37, 187-192 (2005).
  57. 2003年

  58. Sawakami-Kobayashi,K. et al. Multipurpose robot for automated cycle sequencing. Biotechniques 34, 634-637 (2003).

    総説(英文)

  1. Umemura, M., Koike, H. & Machida, M. Motif-independent de novo detection of secondary metabolite gene clusters - toward identification from filamentous fungi. Frontiers Microbiol. 6, 371 (2015).
  2. Machida,M., Yamada,O. & Gomi,K. Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future. DNA Res. 15, 173-183 (2008).
  3. Kobayashi,T. et al. Genomics of Aspergillus oryzae. Biosci. Biotechnol. Biochem. 71, 646-670 (2007).
  4. Payne,G.A. et al. The Aspergilli: Genomics, Medical Aspects, Biotechnology, and Research Methods. Osmani,S.A. & Goldman,G.H. (eds.), pp. 12-24 (CRC Press,2007).
  5. Abe,K., Gomi,K., Hasegawa,F. & Machida,M. Impact of Aspergillus oryzae genomics on industrial production of metabolites. Mycopathologia 162, 143-153 (2006).
  6. J. Yu, R.H. Proctor, D.W. Brown, K. Abe, K. Gomi, M. Machida, F. Hasegawa, W.C. Nierman, D. Bhatnagar. T.E. Cleveland, Genomics of Economically Significant Aspergillus and Fusarium Species, Appl. Mycol. Biotechnol.: Fungal Genomics. Vol. 4., 249-283 (2004)
  7. M. Machida, M. Sano, K. Asai, T. Kumagai1, T. Kin1, H. Nagasaki1, T. Tanaka, et al. (total 28, first), Genome Sequencing of Aspergillus oryzae, The 10th International Congress for Culture Collections, Open Seminar "Traditional Koji mold entering a modern gate" Booklet 44-49 (2004)
  8. M. Machida, Progress of Aspergillus oryzae genomics, Advances in Applied Microbiology, 51, 81-106 (2002)

    総説・解説(邦文)

  1. 町田 雅之、安達 博、「新市場創造のための新技術の研究」の考察、市場創造研究会「市場創造研究」、(2013)
  2. 小池 英明、町田 雅之、第20章 コウジ酸の生合成遺伝子,麹菌培養条件に応答した遺伝子発現機構、発酵・醸造食品の最新技術と機能性Ⅱ(2011)
  3. 町田雅之、麹菌のゲノム解析からわかった新事実、国立科学博物館雑誌『milsil(ミルシル)』、6、15-17 (2008)
  4. 西村麻里江、町田 雅之、小池 英明、糸状菌ゲノム解析のインパクト、化学と生物、46-1、32-40 (2008)
  5. 町田 雅之、神崎壽夫、近赤外蛍光ナノ粒子を利用した生体反応検出、ナノ蛍光体の開発と応用、253-261 (2007)
  6. 町田雅之、新しいSNPsタイピング技術と技術動向、バイオ解析・診断技術のテーラーメイド医療への応用、シー・エム・シー、46-51 (2006)
  7. 町田雅之、麹菌のゲノム解析、蛋白質・核酸・酵素、51, 452-456 (2006)
  8. 町田雅之、麹菌ゲノム解析の完了と今後の展望、BRAINテクノニュース、(2006)
  9. 町田雅之、麹菌のゲノム解析、蛋白質 核酸 酵素,51-5,pp.452-456 (2006)
  10. 町田雅之、阿部敬悦、DNAマイクロアレイ技術の利用、発酵・醸造食品の最新技術と機能性、シー・エム・シー、132-142 (2006)
  11. 町田雅之、麹菌とゲノム解析、化学と教育、日本化学会化学教育協議会(2006)
  12. 町田雅之、麹菌のゲノム解析、Ohm Bulletin、42, 6-7, (2006)
  13. 町田雅之、麹菌ゲノム科学と産業利用、産総研TODAY,6-3,pp.12-15 (2006)
  14. 町田雅之、マイクロビーズを用いた解析技術、Cytometry Research,15,pp.41-45 (2005)
  15. 町田雅之、阿部敬悦、マイクロアレイを用いた遺伝子発現解析、生物工学会誌,83-6,pp.280-282 (2005)
  16. 町田雅之、糸状菌のゲノム解析~麹菌を中心として~、学術月報 (2005)
  17. 町田雅之、麹菌のゲノム解析とその背景、化学・バイオつくば財団ニュース,58,pp.39-42 (2005)
  18. 柏木豊、山口加奈子、栗原洋子、鈴木聡、秋田修、町田雅之、小山泰二、山口庄太郎、五味勝也、阿部敬悦、竹内道雄、小林哲夫、堀内裕之、北本勝ひこ 「麹菌の発現遺伝子の大規模解析」、日本醤油研究所雑誌、30(4),135-141 (2004)
  19. 阿部敬悦、五味勝也、町田雅之、長谷川史彦、「糸状菌(カビ)ゲノム情報の産業利用」ゲノミックス・プロテオミックスの新展開、エヌ・ティー・エス (2004)
  20. 町田雅之、「麹菌のゲノム研究」、伝統ある麹の新しい世界、pp.43-48、2004/10
  21. 町田雅之、「微粒子を用いた高速解析技術」、先端化学シリーズ III 糖鎖/バイオマテリアル/分子認識/バイオインフォマティクス、丸善 日本化学会編 (2003)
  22. 町田雅之、「磁気ビーズを用いたSNPタイピング」、医学のあゆみ、医歯薬出版、206(8), 492-496 (2003)
  23. 町田雅之、「テーラーメード医療を実現するための解析技術」、Bioベンチャー Vol.3 No.3、羊土社 (2003)
  24. 町田雅之、「磁気ビーズを用いた多重化自動SNP解析技術」、バイオサイエンスとインダストリー、財団法人バイオインダストリー協会、60(12), 821-824 (2002)
  25. 五味勝也、阿部敬悦、町田雅之、「糸状菌のゲノム研究のフロントライン」、化学と生物、学会出版センター、Vol.40, No.12, 802-812 (2002)


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生物プロセス研究部門

北海道センター
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札幌市豊平区月寒東2条17丁目2-1
011-857-8537(代)
つくばセンター
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茨城県つくば市東1-1-1 中央第6
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