1. S. Kato, H. Tobe, H. Matsubara, M. Sawada, Y. Sasaki, S. Fukiya, N. Morita and A. Yokota: The membrane phospholipid cardiolipin plays a pivotal role in bile acid adaptation by Lactobacillus gasseri JCM1131T. Biochim. Biophys. Acta 1864, 403-412(2019).


  1. R. Yamamoto, H. Minami, H. Matsusaki, M. Sakashita, N. Morita, O. Nishimiya, N. Tsutsumi, M. Hosokawa, Y. Itabashi, T. Matsui and K. Ura: Consumption of the edible sea urchin Mesocentrotus nudus attenuates body weight gain and hepatic lipid accumulation in mice. J. Func. Foods 47, 40-47(2018).
  2. S. Haga, H. Kanno, T. Ozawa, N. Morita, M. Asano and M. Ozaki: Detection of necroptosis in ligand-mediated and hypoxia-induced injury of hepatocytes using a novel optic probe detecting receptor-interacting protein (RIP)1/RIP3 binding. Oncol. Res. 26, 503-513(2018).
  3. S. Haga, T. Ozawa, N. Morita, M. Asano, S. Jin, Yimin and M. Ozaki: Photo-activatable Akt probe: A new tool to study Akt-dependent physio-pathology of cancer cells. Oncol. Res. 26, 467-472(2018).


  1. S. Mahatabuddin, Y. Haneda, Y. Nishimiya, A. Miura, H. Kondo, P.L. Davies, S. Tsuda: Concentration-dependent oligomerization 1 of an alpha-helical antifreeze polypeptide makes it hyperactive, Scientific Reports, 7, 42501(2017).
  2. T. Adachi, T. Sahara, H. Okuyama and N. Morita: Glass bead-based genetic transformation: An efficient method for transformation of thraustochytrid microorganisms. J. Oleo Sci. 66, 791-795(2017).


  1. M. Igarashi, Q. Zhu, M. Sasaki, R. Kodama, K. Oda, M. Fukushima: Catalytic oxidation of 2,4,6-tribromophenol using iron(III) complexes with imidazole, pyrazole, trizine and pyridine ligands, J. Molecular Catalysis A: Chemical, 413, 100-106(2016).
  2. Q. Zhu, M. Igarashi, M. Sasaki, T. Miyamoto, R. Kodama, M. Fukushima: Degradation and debromination of bromophenols using a free-base porphyrin and metalloporphyrins as photosensitizers under conditions of visible light irradiation in the absence and presence of humic substances, Applied Catalysis B: Environmental, 183, 61-68(2016).
  3. N. Kawashima, Y. Nishimiya, S. Takahata, K. Nakayama: Induction of glycosphingolipid GM3 expression by valproic acid suppresses cancer cell growth, J. Biol. Chem., 291, 21424-21433(2016).
  4. S. Mahatabuddin, Y. Nishimiya, A. Miura, H. Kondo, S. Tsuda: Critical ice shaping concentration (CISC): A new parameter to evaluate the activity of antifreeze proteins, Cryobiology and Cryotechnology, 62, 95-103(2016).


  1. Kanno, M., Katayama, T., Morita, N., Tamaki, H., Hanada, S., and Kamagata, Y. (2015) Catenisphaera adipatacumulans gen. nov., sp. nov., a member of the family Erysipelotrichaceae isolated from an anaerobic digester.  Int. J. Syst. Evol. Microbiol., 65, 805-810.
  2. T. Teranishi, T. Murokawa, M. Enomoto, S. Kuwahara: Synthesis of (±)-terpendole E, Biosci. Biotechnol. Biochem., 79(1), 11-15(2015).
  3. S. Maeno, Q. Zhu, M. Sasaki, T. Miyamoto, M. Fukushima: Monopersulfate oxidation of tetrabromobisphenol A by an iron(III)-phethalocyaninetetrasulfate catalyst coordinated to imidazole functionalized silica particles, J. Molecular Catalysis A: Chemical, 400, 56-63(2015).


  1. Haga, S., Ozawa, T., Yamada, Y., Morita, N., Nagashima, I., Inoue, H., Inaba, Y., Noda, N., Abe, R., Umezawa, K., and Ozaki, M. (2014) p62/SQSTM1 plays a protective role in oxidative injury of steatotic liver in a mouse hepatectomy model. Antioxid. Redox Signal. 21, 2515-2530.
  2. Makino, M., Sahara, T., Morita, N., and Ueno, H. (2014) Amino acid substitution reveals the role of V-shape helix on construction of yeast carboxypeptidase Y. J. Biol. Macromol. 14, 27-41.
  3. Hanada, Y., Nishimiya, Y., Miura, A., Tsuda, S., and Kondo H. (2014) Hyperactive antifreeze protein from an Antarctic sea ice bacterium Colwellia sp. has a compound ice-binding site without repetitive sequences. FEBS J. 281, 3576-3590.
  4. Basu, K., Garnham, C. P., Nishimiya, Y., Tsuda, S., Braslavsky, I., and Davies, P. L. (2014) Determining the ice-binding planes of antifreeze proteins by fluorescence-based ice plane affinity. J. Visual. Exp. 83, e51185.
  5. Katayama, T., Kanno, M., Morita, N., Hori, T., Narihiro, T., Mitani, Y., and Kamagata, Y. (2014) An oleaginous bacterium that intrinsically accumulates long-chain free fatty acids in its cytoplasm. Appl. Environ. Microbiol. 80, 1126-1131.
  6. Narita, T. B., Kikukawa, T. W., Sato, Y. G., Miyazaki, S. H., Morita, N., and Saito, T. (2014) Role of fatty acid synthase on the development of Dictyostelium discoideum. J. Oleo Sci. 63, 281-289.
  7. I. Nagashima, J. Sugiyama, T. Sakuta, M. Sasaki, H. Shimizu: Efficiency of 2.45 GHz and 5.80 GHz microwave irradiation for a hydrolysis reaction by thermostable β-Glucosidase HT1', Biosci. Biotechnol. Biochem., 78(5), 758-760(2014).
  8. M. Enomoto, W. Kitagawa, Y. Yasutake, H. Shimizu: Total synthesis of Aurachins C, D and L, and a structurally simplified analog of Aurachin C, Biosci. Biotechnol. Biochem., 78(8), 1324-1327(2014).


  1. Kanno, M., Katayama, T., Tamaki, H., Mitani, Y., Meng, X.-Y., Hori, T., Narihiro, T., Morita, N., Hoshino, T., Yumoto, I., Kimura, N., Hanada, S., and Kamagata, Y. (2013) Isolation of butanol- and isobutanol-tolerant bacteria and physiological characterization of their butanol tolerance. Appl. Environ. Microbiol. 79, 6998-7005.
  2. Okuda, T., Fukui, A., and Morita, N. (2013) Altered expression of O-GlcNAc-modified proteins in a mouse model whose glycemic status is controlled by a low carbohydrate ketogenic diet. Glycoconj. J. 30, 781-789.
  3. Kamijima, T., Sakashita, M., Miura, A., Nishimiya, Y., and Tsuda, S. (2013) Antifreeze protein prolongs the life-time of insulinoma cells during hypothermic preservation. PLoS One 8, e73643.
  4. Sato, S., Morita, N., Kitamoto, D., and Habe, H. (2013) Expression and characterization of a class III alcohol dehydrogenase gene from Gluconobacter frateurii in the presence of methanol during glyceric acid production from glycerol. J. Oleo Sci. 62, 835-842.
  5. Yamanouchi, T., Xiao, N., Hanada, Y., Kamijima, T., Sakashita, M., Nishimiya, Y., Miura, A., Kondo, H., and Tsuda, S. (2013) Dependence of freeze-concentration inhibition on antifreeze protein. Low Temp. Sci. 71, 91-96.
  6. Morita, T., Kawamura, D., Morita, N., Fukuoka, T., Imura, T., Sakai, H., Abe, M., and Kitamoto, D. (2013) Characterization of mannosylerythritol lipids including hexadecatetraenoic acid produced from cuttlefish oil by Pseudozyma churashimaensis OK96. J. Oleo Sci. 62, 319-327.
  7. Sato, S., Morita, N., Kitamoto, D., Yakushi, T., Matsushita, K., and Habe, H. (2013) Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol. AMB Express 3, 1-7.
  8. Shiraishi, T., Yokota, S.-I., Morita, N., Fukiya, S., Tomita, T., Tanaka, N., Okada, S., and Yokota, A. (2013) Characterization of lipoteichoic acid from Lactobacillus gasseri JCM 1131T: A novel glycolipid anchor structure. Appl. Environ. Microbiol. 79, 3315-3318.
  9. Kumeta, H., Ogura, K., Nishimiya, Y., Miura, A., Inagaki, F., and Tsuda, S. (2013) NMR structure note: a defective isoform and its activity-improved variant of a type III antifreeze protein from Zoarces elongates Kner. J. Biomol. NMR 55, 225-230.
  10. Ideta, A., Aoyagi, Y., Tsuchiya, K., Kamijima, T., Nishimiya, Y., and Tsuda, S. (2013) A simple medium enables bovine embryos to be held for seven days at 4°C. Sci. Rep. 3, 1173.
  11. Hirota, K., Aino, K., Nodasaka, Y., Morita, N., and Yumoto, I. (2013) Amphibacillus indicireducens sp. nov., a facultatively alkaliphile that reduces an indigo dye. Int. J. Syst. Evol. Microbiol. 63, 464-469.
  12. A. Asanuma, M. Enomoto, T. Nagasawa, S. Kuwahara: Synthesis of (±)-lecanindole D, Tetrahedron Lett., 54, 4561-4563 (2013).
  13. I. Nagashima, J. Sugiyama, T. Sakuta, M. Sasaki, H. Shimizu: Efficiency of 2.45 GHz and 5.80 GHz microwave irradiation for a hydrolysis reaction by thermostable Β-Glucosidase HT1', Biosci. Biotechnol. Biochem., in press.
  14. M. Suzuki, K. Ikeda, M. Yamaguchi, S. Kudoh, K. Yokoyama, R. Satoh, D. Ito, M. Nagayama, T. Uchida, K. Gohara: Neuronal cell patterning on a multi-electrode array for a network analysis platform, Biomaterials, 34(21), 5210-5217 (2013).
  15. R. Izumi, T. Matsushita, N. Fujitani, K. Naruchi, H. Shimizu, S. Tsuda, H. Hinou, S. Nishimura: Microwave-assisted solid-phase synthesis of antifreeze glycopeptides, Chem. Eur. J., 19(12), 3913-3920 (2013).
  16. T. Matsushita, N. Ohyabu, N. Fujitani, K. Naruchi, H. Shimizu, H. Hinou, S. Nishimura: Site-specific conformational alteration induced by sialylation of MUC1 tandem repeating glycopeptides at an epitope region for the anti-KL-6 monoclonal antibody, Biochemistry, 52(2), 402-424 (2013).


  1. Garnham, C. P., Nishimiya, Y., Tsuda, S., and Davies, P. L. (2012) Engineering a naturally inactive isoform of type III antifreeze protein into one that can stop the growth of ice. FEBS Lett. 586, 3876-3881.
  2. Asanuma-Date K., Hirano Y., Le N., Sano K., Kawasaki N., Hashii N., Hiruta Y., Nakayama K., Umemura M., Ishikawa K., Sakagami H., and Ogawa H. (2012) Functional regulation of sugar assimilation by N-glycan-specific interaction of pancreatic α-amylase with glycoproteins of duodenal brush border membrane. J. Biol. Chem. 287, 23104-23118.
  3. Okuda, T., and Morita, N. (2012) A very low carbohydrate ketogenic diet prevents the progression of hepatic steatosis caused by hyperglycemia in a juvenile obese mouse model. Nutr. Diabetes 2, e50.
  4. Motohashi, K. A., Morita, N., Kato, A., and Saito, T. (2012) Identification of Des-methyl-DIF-1 methyltransferase in Dictyostelium purpureum. Biosci. Biotech. Biochem. 76, 1672-1676.
  5. Morita, N., Haga, S., Ozawa, T., Remington, S. J., and Ozaki, M. (2012) Bio-imaging of surgical stress: Dynamic analysis of liver oxidative stress and damage. Luminescence 27, 143-145.
  6. M. Enomoto, A. Morita, S. Kuwahara: Total synthesis of the tremorgenic indole diterpene paspalinine, Angew. Chem. Int. Ed. 51, 12833-12836 (2012).
  7. I. Nagashima, T. Mine, T. Yamamoto, H. Shimizu: Efficiency of organic solvents on the ability of α2,3-sialyltransferase from Photobacterium sp. (JT-ISH-224) to control a hydrolysis side reaction, Carbohydr. Res., 358, 31-36 (2012).
  8. K. Yamada, I. Nagashima, M. Hachisu, I. Matsuo, H. Shimizu: Efficient Solid-phase Synthesis of Cyclic RGD peptide under Controlled Microwave Heating. Tetrahedron Lett., 53(9), 1066-1070 (2012).
  9. I. Nagashima, T. Mine, T. Yamamoto, H. Shimizu: Efficiency of organic solvents on the ability of 2,3-sialyltransferase from Photobacterium sp. (JT-ISH-224) to control a hydrolysis side reaction. Carbohydr. Res., 358, 31-36 (2012).


  1. Narita, T. B., Koide, K., Morita, N., and Saito, T. (2011) Dictyostelium hybrid polyketide synthase, SteelyA, produces 4-methyl-5-pentylbenzene-1,3-diol and induces spore maturation. FEMS Microbiol. Lett. 319, 82-87.
  2. Wu, C., Kawasaki, K., Ohgiya, S., and Ohmiya, Y. (2011) Chemical studies on the BRET system between the bioluminescence of Cypridina and quantum dots. Photochem. Photobiol. Sci. 10, 1531-1534.
  3. Fujii, R., Minami, A., Tsukagoshi, T., Sato, N., Sahara, T., Ohgiya, S., Gomi, K., and Oikawa, H. (2011) Total biosynthesis of diterpene aphidicolin, a specific inhibitor of DNA polymerase alpha: Heterologous expression of four biosynthetic genes in Aspergillus oryzae. Biosci. Biotechnol. Biochem. 75, 1813-1817.
  4. Ishiwata, A., Sakurai, A., Nishimiya, Y., Tsuda, S., and Ito, Y. (2011) Synthetic study and structural analysis of the antifreeze agent xylomannan from Upis ceramboides. J. Am. Chem. Soc. 133, 19524-19535.
  5. A. Miura, S. Fukuchi, R. Okabe, M. Fukushima, M. Sasaki, T. Sato: Effect of different fractions of weathered pumice in the formation of humic-like substances. Clay Minerals, 46(9), 637-648 (2011).
  6. S. L. Gaonkar, I. Nagashima, H. Shimizu: Microwave assisted solution phase synthesis of novel 2-[4-[2-N-methyl-2-pyridylamino]ethoxy]phenyl]-5-substituted-1,3,4-oxadiazole library. Org. Chem. Int., doi:10.1155/2011/751894, Volume 2011, Article ID 751894 (2011)
  7. N. T. Hoai, A. Sasaki, M. Sasaki, H. Kaga, T. Kakuchi and T. Satoh: Characterization, and lectin recognition of hyperbranched polysaccharide obtained from 1,6-anhydro-D-hexofuranose. Biomacromolecules, 12, 1891-1899 (2011).
  8. N. T. Hoai, A. Sasaki, M. Sasaki, H. Kaga, T. Satoh and T. Kakuchi: Selective synthesis of 1,6-anhydro-β-D-mannopyranose and -mannofuranose using microwave-assisted heating. Carbohydr. Res., 346, 1747-1751 (2011).
  9. R. Okabe, A. Miura, M. Fukushima, M. Terahara, M. Sasaki, S. Fukuchi and T. Sato: Characterization of an adsorbed humin-like substance on an allophonic soil formed via catalytic polycondensation between catechol and glycine, and its adsorption capability to pentachlorophenol. Chemosphere, 83: 1502-1506 (2011).
  10. R. Saito, Y. Masubuchi, T. Motohashi, S. Kikkawa and M. Sasaki: Microstructure formation during the annealing of iron nitrides. Materials Research Bulletin, 46, 547-550 (2011).
  11. M. Ikegami, Y. Mie, Y. Hirano, M. Suzuki and Y. Komatsu: Size-controlled fabrication of gold nanodome arrays and its application to enzyme electrodes. Colloids & Surfaces A, 384, 388-392 (2011).
  12. M. Yamaguchi, K. Ikeda, M. Suzuki, A. Kiyohara, S. N. Kudoh, K. Shimizu, T. Taira, D. Ito, T. Uchida and K. Gohara: Cell patterning using a template of micro-structured organosilane layer fabricated by vacuum ultraviolet light lithography. Langmuir, 27, 12521-12532 (2011).


  1. Haga, S., Morita, N,, Irani, K., Ogino, T., and Ozaki, M. (2010) p66Shc plays a pivotal role in impaired liver regeneration in aged mice by a redox-dependent mechanism. Lab. Invest. 90, 1718-1726.
  2. Orikasa, Y., Nodasaka, Y., Ohyama, T., Okuyama, H., Ichise, N., Yumoto, I., Morita, N., Wei, M., and Ohwada, T. (2010) Enhancement of the nitrogen fixation efficiency of genetically-engineered Rhizobium with high catalase activity. J. Biosci. Bioeng. 110, 397-402.
  3. Sugano, Y., Kawamura, Y., Goshima, N., Morita, N., and Ohgiya, S. (2010) Addition of amino acid residues to the N-terminus increases expression of the zeocin-resistance protein in Saccharomyces cerevisiae. Biotechnol. Lett. 32, 1515-1521.
  4. Tochigi, Y., Sato, N., Sahara, T., Wu, C., Saito, S., Irie, T., Fujibuchi, W., Goda, T., Yamaji, R., Ogawa, M., Ohmiya, Y., and Ohgiya, S.(2010) A sensitive and convenient yeast reporter assay for high-throughput analysis by using a secretory luciferase from Cypridina noctiluca. Anal. Chem. 82, 5768-5776.
  5. Nishida, T., Hori, R., Morita, N., and Okuyama, H. (2010) Membrane eicosapentaenoic acid is involved in the hydrophobicity of bacterial cells and affects the entry of hydrophilic and hydrophobic compounds. FEMS Microbiol. Lett. 306, 91-96.
  6. Sugihara, S., Hori, R., Nakanowatari, H., Takada, Y., Yumoto, I., Morita, N., Yano, Y., Watanabe, K., and Okuyama, H. (2010) Possible biosynthetic pathways for all cis-3,6,9,12,15,19,22,25,28-hentriacontanoaene in bacteria. Lipids 45, 167-177.
  7. Okuda, T., Nakakita, S. I., and Nakayama, K. (2010) Structural characterization and dynamics of globotetraosylceramide in vascular endothelial cells under TNF-alpha stimulation. Glycoconj. J. 27, 287-296.
  8. Xiao, N., Suzuki, K., Nishimiya, Y., Kondo, H., Miura, A., Tsuda, S., and Hoshino, T. (2010) Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis. FEBS J. 277, 394-403.
  9. Yamawaki, H., Fujihisa, H., Sakashita, M., Honda, K., and Gotoh, Y.(2010)Vibrational and structural study in phase I of Rb3H(SO4)2. PHYSICA B-CONDENSED MATTER 405, 291-295.
  10. S. Fuchuchi, A. Miura, R. Okabe, M. Fukuchima, M. Sasaki, T. Sato: Spectroscopic investigations of humic-like acids formed via polycondensation reactions between glycine, catechol and glucose in the presence of natural zeolites. J. Molecular Structure, 982, 181-186 (2010).


  1. Haga, S., Remington, J. S., Morita, N., Terui, K. and Ozaki, M. (2009) Hepatic ischemia induced immediate oxidative stress after reperfusion and determined the severity of the reperfusion-induced damage. Antioxid. Redox Signal. 11, 2563-2572.
  2. Orikasa, Y., Tanaka, M., Sugihara, S., Hori, R., Nishida, T., Ueno, A., Morita, N., Yano, Y., Yamamoto, K., Shibahara, A., Hayashi, H., Yamada, Y., Yamada, A., Yu, R., Watanabe, K. and Okuyama, H. (2009) pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis. FEMS Microbiol. Lett. 295, 170-176.
  3. Ogami, S., Hijikata, S., Tsukahara, T., Mie, Y., Matsuno, T., Morita, N., Hara, I., Yamazaki, K., Inoue, N., Yokota, A., Hoshino, T., Yoshimune, K. and Yumoto, I. (2009) A novel membrane-anchored cytochrome c-550 of alkaliphilic Bacillus clarkii K24-1U: expression, molecular features and properties of redox potential. Extremophiles 13, 491-504.
  4. Abe H., Takaoka Y., Chiba Y., Sato N., Ohgiya S., Itadani A., Hirashima M., Shimoda C., Jigami Y. and Nakayama K. (2009) Development of valuable yeast strains using a novel mutagenesis technique for the effective production of therapeutic glycoproteins. Glycobiology 19, 428-436.
  5. Ohgiya, Y., Arakawa, F., Akiyama, H., Yoshioka, Y., Hayashi, Y., Sakai, S., Ito, S., Yamakawa, Y., Ohgiya, S., Ikezawa, Z., and Teshima, R. (2009) Molecular cloning, expression, and characterization of a major 38-kd cochineal allergen. J. Allergy Clin. Immunol. 123, 1157-1162.
  6. Kawashima, N., Tsuji, D., Okuda, T., Itoh, K. and Nakayama, K. (2009) Mechanism of abnormal growth in astrocytes derived from a mouse model of GM2 gangliosidosis. J. Neurochem. 111, 1031-1041.
  7. Anas, A., Okuda, T., Kawashima, N., Nakayama, K., Itoh, T., Ishikawa, M. and Biju, V. (2009) Clathrin-Mediated Endocytosis of Quantum DotPeptide Conjugates in Living Cells. ACS Nano. 3, 2419-2429.
  8. Okuda, T., Furukawa, K. and Nakayama, K. (2009) A novel, promoter-based, target specific assay identifies 2-deoxy-d-glucose as an inhibitor of the globotriaosylceramide biosynthesis. FEBS J. 276, 5191-5202.
  9. Takamichi, M., Nishimiya, Y., Miura, A., and Tsuda, S. (2009) Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein. FEBS J. 276, 1471-1479.
  10. Yasuda, K., Takeya, S., Sakashita, M., Yamawaki, H., and Ohmura, R. (2009) Binary Ethanol-Methane Clathrate Hydrate Formation in the System CH4-C2H5OH-H2O: Confirmation of Structure II Hydrate Formation. J. Phys. Chem. 113, 12598-12601.


  1. Yamamoto, S., Sahara, T., Sato, D., Kawasaki, K., Ohgiya, S., Inoue, A. and Ojima, T. (2008) Catalytically important amino acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis. Enzyme Microbial. Biotechnol. 43, 396-402.
  2. Aino, K., Hirota, I., Matsuno, T., Morita, N., Nodasaka, Y., Fujiwara, T., Matsuyama, H., Yoshimune, K. and Yumoto I. (2008) Bacillus polygoni sp. nov., moderate halophilic non-motile obligate alkaliphile isolated from indigo ball. Int. J. Syst. Evol. Microbiol. 58, 120-124.
  3. Ichise, N., Hirota, K., Ichihashi, D., Nodasaka, Y., Morita, N., Okuyama, H. and Yumoto, I. (2008) H2O2 tolerance of Vibrio rumoiensis S-1T is attained owing to the catalase activity of the cells. J. Biosci. Bioeng. 106, 39-45.
  4. Yamahira, K., Hirota, K., Nakajima, K., Morita, N., Nodasaka, Y. and Yumoto, I. (2008) Acinetobacter sp. strain Ths, a novel psychrotolerant and alkalitolerant bacterium that utilizes hydrocarbon. Extremophiles.12, 729-734.
  5. Okuda, T. and Nakayama, K. (2008) Identification and characterization of the human Gb3/CD77 synthase gene promoter. Glycobiology 18, 1028-1035.
  6. Hirano, Y., Nishimiya, Y., Kowata, K., Mizutani, F., Tsuda, S., and Komatsu, Y. (2008) Construction of Time-Lapse Scanning Electrochemical Microscopy with Temperature Control and Its Application to Evaluate the Preservation Effects of Antifreeze Proteins on Living Cells. Anal. Chem. 80, 9349-9354.
  7. Nishimiya, Y., Kondo, H., Takamichi, M., Sugimoto, H., Suzuki, M., Miura, A., and Tsuda, S. (2008) Crystal Structure and Mutational Analysis of Ca2+-Independent Type II Antifreeze Protein from Longsnout Poacher, Brachyopsis rostratus. J. Mol. Biol. 382, 734-746.
  8. Yasui, M., Takamichi, M., Miura, A., Nishimiya, Y., Kondo, H. and Tsuda, S. (2008) Hydroxyl groups of threonines contribute to the activity of Ca2+-dependent type II antifreeze protein. Cryobiology and Cryotechnology 54, 1-8.
  9. Hirano, Y., Nishimiya, Y., Matsumoto, S., Matsushita, M., Todo, S., Miura, A., Komatsu, Y., and Tsuda, S. (2008) Hypothermic preservation effect on mammalian cells of type III antifreeze proteins from notched-fin eelpout. Cryobiology 57, 46-51.
  10. Holland, N. B., Nishimiya, Y., Tsuda, S., and Sonnichsen, F. D. (2008) Two-Domains of RD3 Antifreeze Protein Diffuse Independently. Biochemistry 47, 5935-5941.
  11. Nishimiya, Y., Mie, Y., Hirano, Y., Kondo, H., Miura, A., and Tsuda, S. (2008) Mass preparation and technological development of an antifreeze protein - Toward a practical use of biomolecules -. Synthesiology 1, 7-14.
  12. Yamawaki, H., Fujihisa, H., Sakashita, M., and Honda, K.(2008)Infrared study of proton-deuteron mutual diffusion in a CsHSO4/CsDSO4 solid under high pressure. PHYSICA B-CONDENSED MATTER 403, 2643-2648.


  1. , Nagao, A., Ohmiya, Y. and Ohgiya, S. (2007) Yeast mutant with efficient secretion identified by a novel secretory reporter, Cluc. Biochem. Biophys. Res. Commun. 358, 429-434.
  2. Nishida, T., Morita, N., Yano, Y., Orisaka, Y. and Okuyama, H. (2007) The antioxidative function of eicosapentaenoic acid in a marine bacterium, Shewanella marinintestina IK-1. FEBS Lett. 581, 4212-4216.
  3. Orikasa, Y., Ito, Y., Nishida, T., Watanabe, K., Morita, N., Ohwada, T., Yumoto, I. and Okuyama, H. (2007) Enhanced heterologous production of eicosapentaenoic acid in Escherichia coli cells that coexpress eicosapentaenoic acid biosynthesis pfa genes and foreign DNA fragments including a high-performance catalase gene, vktA. Biotechnol. Lett. 29, 803-809.
  4. Hara, I., Ichise, N., Kojima, K., Kondo, H., Ohgiya, S., Matsuyama, H. and Yumoto, I. (2007) Relationship between size of bottleneck 15 A away from iron in main channel and reactivity of catalase corresponding to melecular size of substrates. Biochemistry 46, 11-22.
  5. Wu, C., Kawasaki, K., Ogawa, Y., Yoshida, Y., Ohgiya, S. and Ohmiya, Y. (2007) Preparation of biotinylated Cypridina luciferase and its use in bioluminescent enzyme immunoassay. Anal. Chem. 79, 1634-1638.
  6. Okuyama, H., Orikasa, Y., Nishida, T., Watanabe, K. and Morita, N. (2007) Bacterial genes responsible for the biosynthesis of eicosapentaenoic and docosahexaenoic acids and their heterologous expression. Appl. Environ. Microbiol. 73, 665-670.
  7. Furukawa, K., Tajima, O., Okuda, T., Tokuda, N., and Furukawa, K. (2007) Knockout mice and glycolipids. COMPREHENSIVE GLYCOSCIENCE 4, 149-157.
  8. Takamichi, M., Nishimiya, Y., Miura, A., and Tsuda, S. (2007) Effect of annealing time of an ice crystal on the activity of type III antifreeze protein. FEBS J. 274, 6469-6476.
  9. Watanabe, H., Tsumoto, K., Taguchi, S., Yamashita, K., Doi, Y., Nishimiya, Y., Kondo, H., Umetsu, M., and Kumagai, I. (2007) A Human Antibody Fragment with High Affinity for Biodegradable Polymer Film. Bioconjugate Chem. 18, 1043-1082.
  10. Holland, N. B., Nishimiya, Y., Tsuda, S., and Sonnichsen, F. D. (2007) Activity of a Two-Domain Antifreeze Protein is Not Dependent on Linker Sequence. Biophys. J. 92, 541-546.
  11. Sakashita, M., Fujihisa, H., Suzuki, KI., Hayashi, S., and Honda, K. (2007) Using X-ray diffraction to study thermal phase transitions in Cs5sub>H3(SO,4),4・ xH,2O. Solid State Ionics 178, 1262-1267.


  1. Orikasa, Y., Nishida, T., Yamada, A., Yu, R., Watanabe, K., Hase, A., Morita, N. and Okuyama, H. (2006) Recombinant production of docosahexaenoic acid in a mode of polyketide biosynthesis in Escherichia coli. Biotechnol. Lett. 28, 1841-1847.
  2. Orikasa, Y., Nishida, T., Hase, A., Watanabe, K., Morita, N. and Okuyama, H. (2006) A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1. FEBS Lett. 580, 4423-4429.
  3. Okamoto, M., Yoko-o, T., Umemura, M., Nakayama, K. and Jigami, Y. (2006) Glycosylphosphatidylinositol-anchored proteins are required for the transport of detergent-resistant microdomain-associated membrane proteins Tat2p and Fur4p. J. Biol. Chem.281, 4013-4023.
  4. Okuda, T., Tokuda, N., Numata, S., Ito, M., Ohta, M., Kawamura, K., Wiels, J., Urano, T., Tajima, O., Furukawa, K. and Furukawa, K. (2006) Targeted disruption of Gb3/CD77 synthase gene resulted in the complete deletion of globo-series glycosphingolipids and loss of sensitivity to verotoxins. J. Biol. Chem.281, 10230-10235.
  5. Furukawa, K., Okuda, T., Furukawa, K. (2006) Roles of glycolipids in the development and maintenance of nervous tissues. Methods Enzymol.15417, 37-52.
  6. Nishimiya, Y., Kondo, H., Yasui, M., Sugimoto, H., Noro, N., Sato, R., Suzuki, M., Miura, A., and Tsuda, S. (2006) Crystallization and preliminary X-ray crystallographic analysis of Ca2+-independent and Ca2+-dependent species of the type II antifreeze protein. Acta CrystallographicaF62, 538-541.
  7. Fujihisa, H., Akahama, Y., Kawamura, H., Ohishi, Y., Shimomura, O., Yamawaki, H., Sakashita, M., Gotoh, Y., Takeya, S., and Honda, K.(2006)O8 cluster structure of the epsilon phase of solid oxygen. PHYSICAL REVIEW LETTERS 97, 085503-1-4.


  1. Kobashigawa, Y., Nishimiya, Y., Miura, K., Ohgiya, S., Miura, A. and Tsuda, S. (2005) A part of ice nuceation protein exhibits an ice-binding ability. FEBS Lett. 579, 1493-1497.
  2. Okada, Y., Sahara, T., Mitsubayashi, H., Ohgiya, S. and Nagashima, T. (2005) Knowledge-assisted recognition of cluster boundaries in gene expression data. Artif. Intell. Med. 35, 171-183.
  3. Yasui, Y., Hosokawa, M., Sahara, T., Suzuki, R., Ohgiya, S., Kohno, H., Tanaka, T. and Miyashita, K. (2005) Bitter gourd seed fatty acid rich in 9c, 11t, 13t-conjugated linolenic acid induces apoptosis and up-regulates the GADD45, p53 and PPARgamma in human colon cancer Caco-2 cells. Prostaglandins Leukot. Essent. Fatty Acids. 73, 113-119.
  4. Tochigi, Y., Yamashiki, N., Ohgiya, S., Ganaha, S. and Yokota, H. (2005) Isoform-specific expression and induction of udp-glucuronosyltransferase in immunoactivated peritoneal macrophages of the rat. Drug Metab. Dispos. 33, 1391-1398.
  5. Morita, N., Ichise, N., Yumoto, I., Yano, Y., Ohgiya, S. and Okuyama, H. (2005) Cultivation of microorganisms in the cultural medium made from squid internal organs and accumulation of polyunsaturated Fatty acids in the cells. Biotechnol. Lett. 27, 933-941.
  6. Morita, N., Nishida, T., Tanaka, M., Yano, Y. and Okuyama, H. (2005) Enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria. Biotechnol. Lett. 27, 389-393.
  7. Saito, T., Kato, A., Ochiai, H. and Morita, N. (2005) Temperature adaptation in Dictyostelium: role of delta 5 fatty acid desaturase. Microbiology 151, 113-119.
  8. Kobashigawa, Y., Nishimiya, Y., Miura, K., Ohgiya, S., Miura, A., and Tsuda, S. (2005) A part of ice nucleation protein exhibits the ice-binding ability. FEBS Letters. 579, 1493-1497.
  9. Nishimiya, Y., Sato, R., Takamichi, M., Miura, A., and Tsuda, S. (2005) Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-fin eelpout, Zoarces elongatus Kner. Eur. J. Biochem. 272, 482-492.
  10. Yamawaki, H., Sakashita, M., Fujihisa, H., and Honda, K.(2005)Infrared study on crystalline and amorphous phases of 2-propyn-1-ol under high pressure. PHYSICA B-CONDENSED MATTER 369, 44-50.



  1. 清水弘樹、マイクロ波反応装置、日刊工業新聞、産総研の企業連携&VB支援、2015.1.15


  1. 森田直樹(2014):農林水産物・食品の機能性評価 〜 核内受容体を利用して評価する 〜. グリーンテクノ情報 10, 6-10.
  2. 尾崎倫孝、芳賀早苗、森田直樹、野田なつみ、小澤岳昌(2014):光学技術を応用した新たな臓器保存法,細胞・臓器移植法の開発. Organ Biology 21, 171-176.
  3. 坂下真実、西宮佳志、近藤英昌、津田栄、山脇浩、藤久裕司、後藤義人(2014):高圧下における魚類由来不凍タンパク質と氷の相互作用. Photon Factory Activity Report 2013 #31B, 197.
  4. 長島生、清水弘樹、最新マイクロ波エネルギーと応用技術、第5章5-3生体高分子合成(糖鎖、ペプチド、糖ペプチド)(株)産業技術サービスセンター、pp. 543-549(2014)


  1. 坂下真実、西宮佳志、近藤英昌、津田栄、山脇浩、藤久裕司、後藤義人(2013):魚類由来III型不凍タンパク質水溶液における圧力効果. Photon Factory Activity Report 2012 #30B, 253.
  2. 加我晴生、人工硬膜材料の開発事例、体内埋め込み医療材料の開発とその理想的な性能・デザインの要件、技術情報協会、pp. 157-163 (2013)


  1. 奥田徹哉、近江谷克裕(2012):抗体の標識/修飾 - 酵素標識 -    新機能抗体開発ハンドブック(浜窪隆雄監修)  (株)エヌ・ティー・エス、205-208.
  2. 坂下真実、津田栄(2012):不凍タンパク質の機能および食品分野への応用    食品と科学 54, 15-18.
  3. 坂下真実、西宮佳志、近藤英昌、津田栄、山脇浩、藤久裕司、後藤義人(2012):魚類由来不凍タンパク質水溶液における圧力効果    高圧力の科学と技術 22, 1-2.
  4. Morita, N.(2012):Molecular and Biological Technology   Agricultural Sciences for Human Sustainability: Meeting the Challenges of Food Safety and Stable Food Production (Graduate School of Agriculture, Hokkaido University Editorial Committee, ed), pp. 55, Kaiseisha Press, Otsu, Shiga, Japan.
  5. 坂下真実、西宮佳志、近藤英昌、津田栄(2012):寒冷な海に生きる魚と不凍タンパク質    生物科学 63, 214-221.
  6. 長島生、清水弘樹 マイクロ波を利用した全合成 -ペプチド、糖鎖、創薬ライブラリ、薬剤プロセス合成、酵素合成研究を通じてマイクロ波非加熱効果に迫る, 有機合成化学協会誌, 3月号(2012).


  1. 古川鋼一、近藤裕史、奥田徹哉、古川圭子(2011):グロボ系糖脂質欠損マウス  生物機能モデルと新しいリソース・リサーチツール Seriesモデル動物利用マニュアル(小幡裕一、城石俊彦編集) (株)エル・アイ・シー、311-313.
  2. 扇谷悟、佐原健彦、栃木裕貴、佐藤なつ子、呉純、近江谷克裕(2011):産業技術総合研究所における酵母を用いた研究の事例 -発現系・レポーターアッセイの実用化,組換え体の安全管理-  生物工学会誌 89, 539-543.
  3. 尾崎倫孝、芳賀早苗、森田直樹、深井原、藤堂省、小澤岳昌、近江谷克裕(2011):移植臓器の非侵襲的モニタリング法の開発  Organ Biology 18, 134-140.
  4. 西宮佳志、津田栄(2011): 不凍タンパク質の技術開発  冷凍 86, 551-556.
  5. 長島生、清水弘樹 バイオ医薬品開発における糖鎖技術、第3編第8章酵素化学合成法による糖鎖生産技術,シーエムシー出版, 85-95, (2011).


  1. 西宮佳志、近藤英昌、坂下真実、三浦愛、津田栄(2010):不凍タンパク質 -機能と応用-  化学と生物 48, 381-388.
  2. 清水弘樹 マイクロ波を利用した糖鎖合成, ファインケミカル2011年5月号、Vol40, No.5,シーエムシー出版, 38-43, (2011).
  3. 加我晴生:非プロトン性極性溶媒を用いるマイクロ波加熱によるアンヒドロ糖の合成,化学工業 61: 884-890 (2010).


  1. 森田直樹(2009):微生物代謝工学に基づく機能性脂質生産を目指す本格研究 ー 機能性脂質の新展開. 産総研における本格研究の展開 時評社、164-169.
  2. 佐原健彦、扇谷悟(2007):出芽酵母における低温条件を利用した蛋白質生産. バイオサイエンスとインダストリー 65, 130-131.
  3. 奥山英登志、折笠善丈、西田孝伸、森田直樹(2007):細菌pfa遺伝子を利用したEPAおよびDHAの生産. バイオサイエンスとインダストリー 65, 188-190.
  4. 奥田徹哉, 徳田典代, 古川圭子, 古川鋼一(2007):細菌毒素と糖脂質受容体:O157感染を中心に. 実験医学増刊号 25, 949-953.
  5. 森田直樹、斉藤玉緒(2006):上巻、Part II、細胞の生物学 7.細胞間の相互作用(原題:chapter 7 Cell-Cell Interactionsの翻訳) 「レーヴン・ジョンソン 生物学」原書第7版 培風館.
  6. 森田直樹(2005):海洋細菌から見出された新たな遺伝子資源 -DHAやEPA合成に関与する遺伝子群-  産総研技術開発カタログー次のヒントはここにあるー 丸善プラネット株式会社、64-65 .
  7. 佐原健彦(2005):酵母低温発現系の開発  産総研技術開発カタログー次のヒントはここにあるー 丸善プラネット株式会社、180-181.


  1. 佐原健彦、扇谷悟、合田孝子、川崎公誠:酵母由来プロモーターならびにこれを用いたベクターおよび発現系.特許第4332630号
  2. 森田直樹、扇谷悟、尾田千佳子:海洋性微生物の培養法.特許第4051448号
  3. 森田直樹、星野保、澤田美智子、奥山英登志、栗木みどり、川上顕、寺見文宏:糸状菌により生産されるセルロース.特許第3607919号
  4. 森田直樹、奥山英登志、湯本勳:高度不飽和脂肪酸及び高度不飽和リン脂質の製造方法.特許第3502903号
  5. 森田直樹、奥山英登志:微生物を用いた高度不飽和脂肪酸の製造方法.特許第3477512号
  6. 星野保、石崎紘三、湯本勳、扇谷悟:低温活性プロテアーゼ及びその製法.特許第2873936号
  7. 特許登録 加我晴生、佐々木正秀、佐々木皇美、奥山市子、覚知豊次、佐藤敏文、ムエン・ト・ホアイ、多分岐ポリマー及びその製造方法、特許第5649161号、2014年11月21日
  8. 特許登録 加我晴生、佐々木正秀、佐々木皇美、鳴海敦、高橋憲司、佐藤宏衣、羽田由衣、佐藤敏文、覚知豊次、アンヒドロ糖の製造方法、特許第5267964号、2013年5月17日
  9. 特許登録 加我晴生、佐々木正秀、佐々木皇美、鳴海敦、金子憲明、高杉友、アンヒドロ糖の製造方法、特許第5131801号、2012年11月16日
  10. 特許登録 高橋憲司、加我晴生、金沢大学、(独)産業技術総合研究所、レボグルコサンの製造方法、特許第4756212号、2011年6月10日.
  11. 特許出願 加我晴生、佐々木正秀、佐々木皇美、奥山市子、覚知豊次、佐藤敏文、ムエン・ト・ホワイ、多分岐ポリマー及びその製造法、特願2010-108168(2010).
  12. 清水弘樹、西村紳一郎、「シアリルラクトサミン系、シアリルルイスX系のシアル酸とフコース含有新規糖 化合物」、 出願人:独立行政法人 産業技術総合研究所
    WO/2011/046057 、公開日:平成23年4月21日


  1. Kawasaki, K., Morita, Y., Ohgiya, S., Ohmiya, Y., and Ohyama, H. : Mutant luciferase, 2452457(英国)
  2. 佐原健彦、扇谷悟、合田孝子、川崎公誠:酵母由来プロモーターならびにこれを用いたベクターおよび発現系.7371847(米国)
  3. 森田直樹、星野保、澤田美智子、奥山英登志,栗木みどり、川上顕、寺見文宏:糸状菌により生産されるセルロース.6958227(米国)

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