Primer sequences used for the test describe in ISO18074 Textiles -- Identification of some animal fibres by DNA analysis method – Cashmere, Wool, Yak and their blends



The sequences of primer sets for the testing described in ISO18074 were written below. These were mentioned in the section 4.9 – 4.12, and 7.21 in the International standard. Annealing temperature of PCR (9.5.2) should be set for 62 ˚C for those primer sets.


4.10 primer for cashmere (DNA fragment of 239 bp should be amplified)
Forward: gtggtagctatttttatctgggctt
Reverse: gctttattttagcactagaaaccgc


4.11 primer for wool (DNA fragment of 385 bp should be amplified)
Forward: ggagtgcacccaggaaagattc
Reverse: ccaaccgaaactgttaactcataaggag


4.12 primer for yak (DNA fragment of 333 bp should amplified)
Forward: gataccgcggccgttaaacag
Reverse: actcctagccccaatactggactaa


7.21 primer amplification verification sample in the reaction solution (DNA fragment of 480 bp should be amplified)
Forward: gaagctgctgcctgatactgac
Reverse: gtcctttttgagttcattcgtaggctag



Contact person:
Hideaki Koike, PhD
Project Leader of ISO18074
Senior researcher, Bioproduction Research Institute,
National Institute of Advanced Industrial Science and Technology (AIST)
e-mail: hi-koike(at)aist.go.jp

Yoichiro Yoshioka
BOKEN Quality Evaluation Institute
e-mail: yosioka(at)boken.or.jp


(at) should be @.

Hokkaido

  • Plant Molecular Technology Research Group
  • Molecular and Biological Technology Research Group
  • Biomolecular Engineering Research Group
  • Applied Molecular Microbiology Research Group
  • Environmental Biofunction Research Group
  • Bio-material Engineering Research Group
  • Antifreeze Protein Research Team
  • Molecular Systems Bioengineering Research Team

Tsukuba

  • Symbiotic Evolution and Biological Functions Research Group
  • Microbial and Genetic Resources Research Group
  • Synthetic Bioengineering Research Group
  • Plant Gene Regulation Research Group
  • Bio-Design Research Group

Director

  • Director's Research

LINK

AIST HOKKAIDO CENTER
AIST

*HOME
*About BPRI
*Research
*Publications
*Information
*Other

AIST Hokkaido
062-8517
2-17-2-1, Tsukisamu-Higashi,
Toyohira Ward, Sapporo City,
Hokkaido, 062-8517 Japan
+81-11-857-8537
AIST Tsukuba Central 6
305-8566
Central 6,1-1-1 Higashi, Tsukuba, Ibaraki 305-8566 Japan
+81-29-861-6040