Primer sequences used for the test describe in ISO18074 Textiles -- Identification of some animal fibres by DNA analysis method – Cashmere, Wool, Yak and their blends
The sequences of primer sets for the testing described in ISO18074 were written below. These were mentioned in the section 4.9 – 4.12, and 7.21 in the International standard. Annealing temperature of PCR (9.5.2) should be set for 62 ˚C for those primer sets.
4.10 primer for cashmere (DNA fragment of 239 bp should be amplified)
4.11 primer for wool (DNA fragment of 385 bp should be amplified)
4.12 primer for yak (DNA fragment of 333 bp should amplified)
7.21 primer amplification verification sample in the reaction solution (DNA fragment of 480 bp should be amplified)
Hideaki Koike, PhD
Project Leader of ISO18074
Senior researcher, Bioproduction Research Institute,
National Institute of Advanced Industrial Science and Technology (AIST)
BOKEN Quality Evaluation Institute
(at) should be @.