不凍蛋白質特別研究チーム【生物プロセス研究部門】
生体物質工学研究グループ
成果発表

査読論文

  • (82) Azuma, N., Miyazaki Y., Nakano, M., and Tsuda, S.: Unexpected Rise of Glass Transition Temperature of Ice Crystallized from Antifreeze Protein Solution. J. Phys. Chem. Lett. 9, 4512-4515 (2018).
  • (81)Mahatabuddin S., Fukami, D., Arai,T., Nishimiya, Y., Shimizu, R., Shibazaki, C., Kondo, H., Adachi, M., and Tsuda, S.: Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein. Proc. Natl. Acad. Sci. USA,115 (21), 5456-5461 (2018).
  • (80) Orii, R., Sakamoto, N., Fukami, D., Tsuda, S., Izumi, M., Kajihara, Y., and Okamoto, R. Total Synthesis of O-GalNAcylated Antifreeze Glycoprotein using the Switchable Reactivity of Peptidyl-N-pivaloylguanidine. Chem. Eur. J., 9253-9257 (2017).
  • (79) Mahatabuddin, S., Hanada, Y., Nishimiya, Y., Miura, A., Kondo, H., Davies, P.L., and Tsuda, S. : Concentration-dependent oligomerization of an alpha-helical antifreeze polypeptide makes it hyperactive. Scientific Reports 7, 42501 (2017).
  • (78) Cheng, J., Hanada, Y., Miura, A., Tsuda, S., and Kondo, H.: Hydrophobic Ice-Binding Sites confer Hyperactivity of an Antifreeze Protein from a Snow Mold Fungus. Biochem. J. 473 (21), 4011-26 (2016).
  • (77) Tsuda, S.: Mass preparation of fish antifreeze protein. J. Bioprocess Biotechniq. 6 (7), 30 (2016).
  • (76) Mahatabuddin S., Nishimiya, Y., Miura, A., Kondo, H., and Tsuda, S.: Critical Ice Shaping Concentration (CISC): A New Parameter to Evaluate the Activity of Antifreeze Proteins. Cryobiology and Cryotechnology 62 (2), 95-103 (2016).
  • (75) Arai, A., Cheng, J., Mahatabuddin, S., Kondo, H., and Tsuda, S.: Observation of Inhibitory Effect of Antifreeze Protein on Progressive Freeze-Concentration. Cryobiology and Cryotechnology 61 (2) 121-124 (2015).
  • (74) Ideta, A., Aoyagi, Y., Tsuchiya, K., Nakamura, K., Shirasawa, A., Sakaguchi, K., Tominaga, N., Nishimiya, Y., and Tsuda, S. : Prolonging hypothermic storage (4oC) of bovine embryos with fish antifreeze protein. J. Reprod. Dev. 61, 1-6 (2015).
  • (73) Hanada, Y., Nishimiya Y., Miura, A., Tsuda, S., and Kondo, H. : Hyperactive antifreeze protein from an Antarctic sea ice bacterium Colwellia sp. has a compound ice-binding site without repetitive sequences. FEBS J. 281 3576-3590 (2014).
  • (72) Xiao, N., Hanada, Y., Seki, H., Kondo, H., Tsuda, S., and Hoshino, T. : Annealing condition influences thermal hysteresis of fungal type ice-binding proteins. Cryobiology 68 159-161 (2014).
  • (71) Singh, P., Hanada, Y., Singh, S., and Tsuda, S. : Antifreeze protein activity in Arctic Cryoconite bacteria. FEMS Microbiol Lett. 351, 14-22 (2014).
  • (70) Sakaki, K., Cristov N., Tsuda, S., and Imai, R. : Identification of a novel LEA protein involved in freezing tolerance in wheat. Plant and Cell Physiology, 55 (1) 136-147 (2014).
  • (69) Tsuchiya, K., Ideta, A., Nishimiya, Y., and Tsuda, S. and Aoyagi, Y. : Artificial dormancy of bovine embryos for a maximum of 7 days using a simple medium. Reproduction, Fertility and Development, 26 (1) 139-140 (2013).
  • (68) Basu, K., Garnham, C.P., Nishimiya, Y., Tsuda, S., Braslavsky, I., and Davies, P.L. : Determining the ice-binding planes of antifreeze proteins by fluorescence-based ice plane affinity. Journal of Visualized Experiments 83, e51185 (2014).
  • (67) Fukami, D., Hanada, Y., Cheng, J., Tsuda, S., and Kondo, H. : Functional Analysis of Antifreeze Protein from Ascomycete. Cryobiology and Cryotechnology 59 (2), 157-160 (2013).
  • (66) Kamijima, T, Sakashita, M., Miura, A., Nishimiya, Y., and Tsuda, S. :Antifreeze Protein Prolongs the Life-Time of Insulinoma Cells during Hypothermic Preservation. PLoS ONE 8 (9), e73643 (2013).
  • (65) Tsuji, M., Fujiu, S., Xiao N, Hanada, Y., Kudoh, S., Kondo, H., Tsuda, S.,and Hoshino, T. : Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica. FEMS Microbiol Lett 346 121-130 (2013).
  • (64) Ideta, A., Aoyagi, Y., Tsuchiya, K., Kamijima, T., Nishimiya, Y., and Tsuda, S. : A simple medium enables bovine embryos to be held for seven days at 4 ℃. Scientific Reports, 3 (1173) 1-5 (2013).
  • (63) Fukushima, M., Tsuda, S., and Nishizawa, Y.-I. : Fabrication of highly porous alumina prepared by gelation freezing route with antifreeze protein. J. Am. Ceram. Soc., 96 (4), 1029-1031 (2013).
  • (62) Kumeta, H., Ogura, K., Nishimiya, Y., Miura, A., Inagaki, F., and Tsuda, S. : A defective isoform and its activity-improved variant of a type III antifreeze protein from Zoarces elongatus Kner. J. Biomol. NMR, 55 (2), 225-230 (2013).
  • (61) Izumi, R., Matsushita, T., Ohyabu, N., Fujitani, N., Naruchi, K., Shimizu, H., Tsuda, S., Hinou, H., and Nishimura, S.-I. : Microwave-assisted solid-phase synthesis of antifreeze glycopeptides. Chem. Eur. J.,19, 3913-3920 (2013).
  • (60) Yamanouchi, T., Xiao, N., Hanada, Y., Kamijima, T., Sakashita, M., Nishimiya, Y., Miura, A., Kondo, H., and Tsuda, S. : Dependence of freeze-concentration inhibition on antifreeze protein. Low Temperature Science 71, 91-96 (2013).
  • (59) Garnham, C.P., Nishimiya, Y., Tsuda, S., and Davies, P.L. : Engineering a naturally inactive isoform of type III antifreeze protein into one that can stop the growth of ice. FEBS Letters, 586, 3876-3881 (2012).
  • (58) Kondo, H., Hanada, Y., Hoshino, T., Garnham, C.P., Davies, P.L., and Tsuda, S. : Ice-binding site of snow mold fungus antifreeze protein deviates from structural regularity and high conservation. Proc. Natl. Acad. Sci., 109 (24), 9360-9365 (2012).
  • (57) Ishiwata, A., Sakurai, A., Nishimiya, Y., Tsuda, S., and Ito, Y. : Synthetic study and structural analysis of the antifreeze agent xylomannan from Upis ceramboides. J. Am. Chem. Soc. 133, 19524-19535 (2011).
  • (56) Xiao, N., Suzuki, K., Nishimiya, Y., Kondo, H., Miura, A., Tsuda, S., and Hoshino, T. : Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis. FEBS J. 277, 394-403 (2010).
  • (55) Yamawaki, H., Fujihisa, H., Sakashita, M., Honda, K., and Gotoh, Y. : Vibration and structural study in phase I of Rb3H(SO4)2. Physica B-Condensed Matter, 405, 291-295 (2010).
  • (54) Yaoi, K., Kondo, H., Hiyoshi, A., Noro, N., Sugimoto, H., Tsuda, S., and Miyazaki, K. : The crystal structure of a xyloglucan-specific endo-beta-1,4-glucanase from Geotrichum sp. M128 xyloglucanase reveals a key amino acid residue for substrate specificity. FEBS J. 276, 5094-5100 (2009).
  • (53) Yasuda, K., Takeya, S., Sakashita, M., Yamawaki, H., and Ohmura, R. : Binary Ethanol-Methane Clathrate Hydrate Formation in the System CH4-C2H5OH-H2O: Confirmation of Structure II Hydrate Formation. J. Phys. Chem. C, 113, 12598-12601 (2009).
  • (52) Yasuda, K., Takeya, S., Sakashita, M., Yamawaki, H., and Ohmura, R. : Characterization of the Clathrate Hydrate Formed with Methane and Propan-1-ol. Ind. Eng. Chem. Res. 48, 9335-9337 (2009).
  • (51) Kodama, Y., Masaki, K., Kondo, H., Suzuki, M., Tsuda, S., Nagura, K., Shimba, N., Suzuki, E. and Iefuji, H. : Crystal Structure and Enhanced Activity of a Cutinase-like Enzyme from Cryptococcus sp. Strain S-2. Proteins 77 (3), 710-717 (2009).
  • (50) Sakae, Y., Matsubara, T., Aida, M., Kondo, H., Masaki. K., and Iefuji, H. : ONIOM Study of the Mechanism of the Enzymatic Hydrolysis of Biodegradable Plastics. Bull. Chem. Soc. Jpn. 82 (3), 338-346 (2009).
  • (49) Hachisu, M., Hinou, H., Takamichi, M., Tsuda, S., Koshida, S., and Nishimura, S.-I.. : One-pot synthesisof cyclic antifreeze glycopeptides. Chem. Commun. 1641-1643 (2009).
  • (48) Takamichi, M., Nishimiya, Y., Miura, A., and Tsuda, S. : Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein. FEBS J. 276 1471-1479 (2009).
  • (47) Nishimiya, Y., Kondo, H., Sugimoto, H., Suzuki, M., Takamichi, M., Miura, A., and Tsuda, S. : Crystal Structure and Mutatiuonal Analysis of Ca2+-independent Type II Antifreeze Protein from Longsnout poacher, Brachyopsis rostatus. J. Mol. Biol., 382 (3), 734-746 (2008).
  • (46) Warashina, A., Hirano, Y., Takamichi, M., Nishimiya, Y., Kondo, H., and Tsuda, S. : Hypothermic preservation of cultured cells with using fish type III antifreeze protein from Notched-fin eelpout. Cryobiology and Cryotechnology 54 (2). 93-96 (2008).
  • (45) Iwasaki, K., Kondo, H., Nishimiya, Y., Takamichi, M., Miura, A., and Tsuda, S. : Enhancement of the activity of antifreeze protein by addition of a water-soluble polymer. Cryobiology and Cryotechnology 54 (2). 89-92 (2008).
  • (44) Hirano, Y., Nishimiya, Y., Kowata, K., Mizutani, F., Tsuda, S., and Komatsu, Y. : Construction of Time-Lapse Scanning Electrochemical Microscopy with Temperature Control and Its Application to Evaluate the Preservation Effects of Antifreeze Proteins on Living Cells. Anal. Chem. 80, 9349-9354 (2008).
  • (43) Nishimiya, Y., Mie, Y., Hirano, Y., Kondo, H., Miura, A., and Tsuda, S. : Mass preparation and technological development of an antifreeze protein - Toward a practical use of biomolecules ? Synthesiology 1 (1), 7-14 (2008).
  • (42) Yasui, M., Takamichi, M., Miura, A., Nishimiya, Y., Kondo, H., and Tsuda, S. : Hydroxyl groups of threonines contribute to the activity of Ca2+-dependent type II antifreeze protein. Cryobiology and Cryotechnology 54 (1). 1-8 (2008).
  • (41) Nakanishi, T., Tsumoto, K., Yokota, A., Kondo, H., and Kumagai, I. : Critical contribution of VH-VL interaction to rehaping of an antibody: The case of humanization of anti-lysozyme antibody, HyHEL-10. Protein Sci. 17 (2), 261-270 (2008).
  • (40) Makabe, K., Nakanishi, T., Tsumoto, K., Tanaka, Y., Kondo, H., Umezu, M., Sone, Y., Asano, R., and Kumagai, I. : Thermodynamic consequences of mutations in Vernier Zone residues of a humanized anti-human epidermal growth factor receptor (EGFR) murine antibody, 528. J. Biol. Chem. 283 (2), 1156-1166 (2008).
  • (39) Sakashita, M., Fujihisa, H., Suzuki, K., Hayashi, S., and Honda, K. : Using X-ray diffraction to study thermal phase transitions in CS5H3(SO4)4 xH2O. Solid State Ionics, 178, 1262-1267 (2007).
  • (38) Takamichi, M., Nishimiya, Y., Miura, A., and Tsuda, S. : Effect of annealing time of an ice crystal on the activity of type III antifreeze protein. FEBS J. 274, 6469-6476 (2007).
  • (37) Matsumoto, S., Matsushita, M., Nishimiya, Y., Hirano, Y., Tsuda, S. and Todo, S. : Type III antifreeze protein extremely enhances viability of cultured endothelial cells during hypothermic preservation. Am. J. Transplant, 7, 506 (2007).
  • (36) Yaoi, K., Kondo, H., Hiyoshi, A., Noro, N., Sugimoto, H., Tsuda, S., Mitsuishi, Y., and Miyazaki, K. : The Structural Basis for the Exo-mode of Action in GH74 Oligoxyloglucan Reducing End-speicific Cellobiohydrolase. J. Mol. Biol., 370, 53-62 (2007).
  • (35) Shiroishi, M., Tsumoto, K., Tanaka, Y., Yokota, A., Nakanishi, T., Kondo, H., and Kumagai, I. : Structural consequences of mutations in interfacial Tyr residues of a protein antigen-antibody complex: The case of HyHEL-10-HEL. J. Biol. Chem. 282, 6783-6791 (2007).
  • (34) Hara, I., Ichise, N., Kojima, K., Kondo,H., Ohgiya, S., Matsuyama, H., and Yumoto, I. : Relationship between Size of Bottleneck 15 Angstrome Away from Iron in Main Channel and Reactivity of Catalase Corresponding to Molecular Size of Substrates. Biochemistry, 46, 11-22 (2007).
  • (33) Uchida, T., Ikeda, I.Y., Ohmura, R., and Tsuda, S. : Effects of additives on formation rates of CO2 hydrate Films. Proceedings of 11th International Conference of Physical Chemistry of Ice, 609-618 (2007).
  • (32) Holland, N.B., Nishimiya, Y., Tsuda, S., and Sonnichsen, F.D. : Activity of a Two-domain Antifreeze Protein is Not Dependent on Linker Sequence. Biophys. J., 46, 11-22 (2007).
  • (31) Nishimiya, Y., Kondo, H., Yasui, M., Sugimoto, H., Noro, N., Sato, R., Suzuki, M., Miura, A., and Tsuda, S. : Crystallization and preliminary X-ray crystallographic analysis of Ca2+-independent and Ca2+-dependent species of the type II antifreeze protein. Acta Crystallographica., F62, 538-541 (2006).
  • (30) Miyazaki, K., Takenouchi M., Kondo, H., Noro, N., Suzuki, M., and Tsuda, S. : Thermal Stabilization of Bacillus Subtilis Family-11 Xylanase by Directed Evolution. J. Biol. Chem., 281, 10236-10242 (2006).
  • (29) Kobashigawa, Y., Nishimiya, Y., Miura, K., Ohgiya, S., Miura, A., and Tsuda, S. : A part of ice nucleation protein exhibits the ice-binding ability. FEBS Letters., 579(6), 1493-1497 (2005).
  • (28) Nishimiya, Y., Sato, R., Takamichi, M., Miura, A., and Tsuda, S. : Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-fin eelpout, Zoarces elongatus Kner. FEBS J. (Eur. J. Biochem.), 272, 482-492 (2005).
  • (27) Yaoi, K., Kondo, H., Noro, N., Suzuki, M., Tsuda, S., and Mitsuishi, Y. : A part of ice nucleation protein exhibits the ice-binding ability. FEBS Letters., 579(6), 1493-1497 (2005).
  • (26) Tachibana, Y., Fletcher, G.L., Fujitani, N., Tsuda, S., Monde, K., and Nishimura, S.-I.: Antifreeze Glycoproteins: Elucidation of the Structural Motifs That Are Essential for Antifreeeze Activity. Angew. Chem. Int. Ed., 43, 856-862 (2004).
  • (25) Hoshino, T., Kiriaki, M., Ohgiya, S., Fujiwara, M., Kondo, H., Nishimiya, Y., Yumoto, I., and Tsuda, S. : Antifreeze proteins from snow mold fungi. Can J. Bot., 81, 1175-1181 (2003).
  • (24) Nishimiya, Y., Ohgiya, S., and Tsuda, S. : Artificial Multimers of The Type III Antifreeze Protein: Effects on Thermal Hysteresis and Ice Crystal Morphology. J. Biol. Chem., 278(34), 32307-32312 (2003).
  • (23) Yaoi, K., Kondo, H., Suzuki, M., Noro, N., Tsuda, S., and Mitsuishi, Y. : Crystallization and Preliminary X-Ray Crystallographic Study on Xyloglucan-Specific exo-beta-glycosidase, oligoxyloglucan reducing-end specific cellobiohydrolase. Acta Crystal., D59, 1838-1839 (2003).
  • (22) Yamashita, Y., Miura, R., Takemoto, Y., Tsuda, S., Kawahara, H., and Obata, H. : Type II Antifreeze Protein from a Mid-Latitude Freshwater Fish, Japanese Smelt (Hypomesus nipponesis). Biosci. Biotech. Biochem., 67(3), 461-466 (2003).
  • (21) Kumeta, H., Miura, A., Kobashigawa, Y., Miura, K., Oka, C., Nemoto, N., Nitta, K., and Tsuda, S : Low-Temperature-Induced Structural Changes in Human Lysozyme Elucidated by Three-Dimensional NMR Spectroscopy. Biochemistry, 42(5), 1209-1216 (2003).
  • (20) Tachibana, Y., Matsubara, N., Nakajima, F., Tsuda, T., Tsuda, S., Monde, K., and Nishimura, S-I. : Efficient and versatile synthesis of mucin-like glycoprotein mimics. Tetrahedron, 58, 10213-10224 (2002).
  • (19) Koike, M., Okamoto, T., Tsuda, S., and Imai, R. : A novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation. Biochecm. Biophys. Res. Commun., 298, 46-53 (2002).
  • (18) Suetake, T., Aizawa, T., Koganesawa, N., Osaki, T., Kobashigawa, Y., Demura, M., Kawabata, S., Kawano, K., Tsuda, S., and Nitta, K. : Production and Characterization of Recombinant tachycitin, the Cys-rich chitin-binding protein. Protein Engineering, 15(9), 763-769 (2002).
  • (17) Kawasaki, K., Kondo, H., Suzuki, M., Ohgiya, S., and Tsuda, S. : Alternate conformations observed in catalytic serine of Bacillus subtilis lipase determined at 1.3 angstrome resolution. Acta Cryst., D58, 1168-1174 (2002).
  • (16) Kumeta, H., Kobashigawa, Y., Miura, K., Nishimiya, Y., Oka, C., Nemoto, N., Miura, A., Nitta, K., and Tsuda, S: Assignment of 1H, 13C, and 15N resonances of human lysozyme at 4oC. J. Biomol. NMR, 22(3), 183-184 (2002).
  • (15) Uedaira Hisashi, Okouchi S., Tsuda, S., and Uedaira Hatsuho. : Hydration of Glucose and Galactose Derivatives. Bull. Chem. Soc. Jpn., 74, 1857-1861 (2001).
  • (14) Kobashigawa, Y., Miura, K., Demura, M., Nemoto, N., Koshiba, T., Nitta, K., and Tsuda, S. : Assignment of 1H, 13C, and 15N resonances of canine milk lysozyme. J. Biomol. NMR, 19(4), 387-388 (2001).
  • (13) Miura, K., Ohgiya, S., Hoshino, T., Nemoto, N., Suetake, T., Miura, A., Spyracopoulos, L., and Tsuda, S. : NMR Analysis of Type III Antifreeze Protein Intramolecular Dimer: Structural Basis for Enhanced Activity. J. Biol. Chem., 276(2), 1304-1310 (2001).
  • (12) Miura, K., Ohgiya, S., Hoshino, T., Nemoto, N., Nitta, K., and Tsuda, S. : Assignments of 1H, 13C, and 15N NMR resonances of intramolecular dimer antifreeze protein RD3. J. Biomol. NMR, 16, 273-274 (2000).
  • (11) Suetake, T., Tsuda, S., Kawabata, S., Kawano, K., Miura, K., Iwanaga, S., Hikichi, K., and Nitta, K. : Chitin-binding Proteins in Invertebrates and Plants Comprise a Common Chitin-binding Structural Motif. J. Biol. Chem., 275(24), 17929-17932 (2000).
  • (10) Hoshino, T., Odaira, T., Yoshida, M., and Tsuda, S. : Physiological and biochemical significance of antifreeze substances in plants. J. Plant Res., 112, 255-261 (1999).
  • (9) Miura, K., Ohgiya, S., Hoshino, T., Nemoto, N., Nitta, K., and Tsuda, S. : Determination of the solution structure of the N-domain plus linker of antarctic eel pout antifreeze protein RD3. J. Biochem., 126, 387-394 (1999).
  • (8) Tsuda, S., Miura, A., Gagne, S.M., Spyracopoulos, L., and Sykes, B.D. : Low-Temperature-Induced Structural Changes in The Apo Regulatory Domain of Skeletal Muscle Troponin C. Biochemistry, 38(18), 5693-5700 (1999).
  • (7) Gagne, S.M., Tsuda, S., Spyracopoulos, L., and Sykes, B.D. : Backbone and Methyl Dynamics of the Regulatory Domain of Troponin C: Anisotropic Rotational Diffusion and Contribution of Conformational Entropy to Calcium Affinity. J. Mol. Biol., 278, 667-686 (1998).
  • (6) Tsuda, S., Ito, A., and Matsushima, N. : A hairpin-loop conformation in tandem repeat sequence of the ice nucleation protein revealed by NMR spectroscopy. FEBS Letters, 409, 227-231 (1997).
  • (5) John, G., Tsuda, S., and Morita, M. : Synthesis and Modification of New Biodegradable Co-polymers: Serine/Glycolic acid Alternating Polymer abd Copolymers of L-Lactide or e-Caprolactone. J. Polym. Sci., A62, 1901-1907 (1997).
  • (4) Ohki, S., Miura, K., Saito, M., Nakashima, K., Maekawa, H., Yazawa, M., Tsuda, S., and Hikichi, K. : Secondary Structure and Ca2+-Binding Property of the N-Terminal Half Domain of Calmodulin from Yeast Saccaromyces cerevisiae as Studied by NMR. J. Biochem., 119, 1045-1055 (1996).
  • (3) Sykes, B.D., Audette, G., Gagne, S.M., Li, M.X., Slupsky, C.M., and Tsuda, S. : NMR Studies of the Calcium-Induced Structural lChanges that Triggers Muscle Contraction. Proceedings of 34th. Hanford Symposium on Health and the Environment, "Biomolecules: From 3-D Structure to Applications"(ed. Ornstein, R.L.) pp. 11-19, Pasco, Washington (1995).
  • (2) Gagne, S.M., Tsuda, S., Li, M.X., Smillie, L.B., and Sykes, B.D. : Structures of the troponinC regulatory domains in the apo and calcium-saturated states. Nature Struct. Biol., 2, 784-789 (1995).
  • (1) Li, M.X., Gagne, S.M., Tsuda, S., Kay, C.M., Smillie, L.B., and Sykes, B.D. : Calcium Binding to the Regulatory N-Domain of Skeletal Muscle Troponin C Occurs in a Stepwise manner. Biochemistry, 34, 8330-8440 (1995).

日本語総説等

  • (22) 津田 栄(監修): 不凍タンパク質の機能と応用 Functions and Applications of Antifreeze Protein. シーエムシー出版 約290頁. ISBN: 978-4-7813-1339-9.
  • (21) 近藤英昌、津田 栄: 氷の結晶成長を止める新しい不凍タンパク質—食品の品質、細胞の生命力を維持できる凍結技術の実現への期待—、化学、72 (8), 49-52 (2017).
  • (20) 岡田璃生、新井達也、津田 栄、佐々木裕次: タンパク質動態からの不凍機能X線1分子解析、低温生物工学会誌、63 (2),85-88 (2017).
  • (19) 津田 栄: 不凍液、日本再生医療学会雑誌 再生医療、15 (4), 372-373 (2016).
  • (18) 津田 栄: 不凍タンパク質の構造と機能 ー高品質魚類不凍タンパク質がもたらす冷熱技術イノベーションー、化学と工業、69 (8), 646-648 (2016).
  • (17) 石原和成、三浦 愛、津田 栄: 魚類不凍蛋白質の細胞保護機能の解析、バイオインダストリー、31 (6), 61-66 (2014).
  • (16) 近藤英昌、津田 栄: キノコの不凍タンパク質の分子構造と不凍機能のメカニズム、化学と生物(日本農芸学会)、52 (1), 10-12 (2014).
  • (15) 坂下真実、西宮佳志、近藤英昌、津田 栄、山脇 浩、藤久裕司、後藤義人: 魚類由来III型不凍タンパク質水溶液における圧力効果、Photon Factory Activity Report 2012 #30 B (2013).
  • (14) 津田 栄: 国産魚類由来の不凍タンパク質、極限生物学会誌、11 (2), 64-69 (2012).
  • (13) 近藤英昌、津田 栄: 不凍タンパク質の探索・解明と応用、極限環境生物の産業展開 シーエムシー出版(監修:今中忠行)、121-129 (2012).
  • (12) 坂下真実、津田 栄: 不凍タンパク質の機能および食品分野への応用、食品と科学, 54 (5), 15-18 (2012).
  • (11) 坂下真実、西宮佳志、近藤英昌、津田 栄: 寒冷な海に生きる魚と不凍タンパク質、生物科学, 63 (4), 214-221 (2012).
  • (10) 近藤英昌、津田 栄: 不凍タンパク質の種類と凍結抑制効果、冷凍(特集:不凍タンパク質の新展開), 86 (1005), 557-561 (2011).
  • (9) 西宮佳志、津田 栄: 不凍タンパク質の技術開発、 冷凍(特集:不凍タンパク質の新展開), 86 (1005), 551-556 (2011).
  • (8) 西宮佳志、近藤英昌、坂下真実、三浦 愛、津田 栄: 不凍タンパク質 ー機能と応用ー、化学と生物、48 (6), 381-388 (2010).
  • (7) 山崎 悠、四ッ倉典滋、江端弘樹、近藤英昌、伊藤 等、嶌田 智 : 高濃度CO2海水下における海草類の生育特性, Algal Resources, 1 (2), 39-44 (2009).
  • (6) 津田 栄 : 不凍タンパク質, タンパク質の辞典 (朝倉書店), pp. 729 (2008).
  • (5) 西宮佳志、三重安弘、平野 悠、近藤英昌、三浦 愛、津田 栄 : 不凍蛋白質の大量精製と新たな応用開拓 - 実用化を指向する蛋白質研究 -, Synthesiology, 1巻 1号, 7-14 (2008).
  • (4) 津田 栄、三浦 愛、西宮佳志:日本産魚類由来の不凍蛋白質. 日本高分子学会誌 特集:海洋生物資源と高分子、高分子 55巻、7月号、494-495 (2006).
  • (3) 津田 栄(取材協力):不凍タンパク質. 日経BP出版センター刊 "産総研のすごい仕事"第1章ライフサイエンス分野、pp. 54-57 (2006).
  • (2) 津田 栄:純国産不凍タンパク質. バイオサイエンスとインダストリー、62(10), 9-10 (2004).
  • (1) 田中正太郎、小橋川敬博、三浦和紀、西宮佳志、三浦 愛、津田 栄:不凍蛋白質. 日本生物物理学会誌 総説、43(3), 130-135 (2003).

招待・依頼講演

  • (53) 津田 栄:「不凍タンパク質の分子機能と技術応用」、石川県立大学食品科学科公開セミナー.金沢 (2017/12/4).
  • (52) 津田 栄:「不凍タンパク質とは何か?−分子機能解明から産業医学応用まで−」、防衛大学校応用化学科課外講演.横須賀(2017/11/28).
  • (51) 津田 栄:「不凍タンパク質の分子機能と技術応用」、第15回Spring-8先端研究ワークショップ.京都 (2017/8/24).
  • (50) 津田 栄:「不凍タンパク質の分子生物学」、日本魚類学会年会.函館 (2017/9/18).
  • (49) Tsuda, S.:A new type I antifreeze protein BpAFP undergoes oligomerization to bind to whole surface of an ice crystal. 2nd International Conference on Bioscience. London, UK (2017/6/19).
  • (48) 津田 栄:「不凍物質の科学」、ちよだプラットホームスクエア 第3回低温科学技術交流調査会.東京 (2017/1/20).
  • (47) 津田 栄:「高純度魚類不凍タンパク質を用いた細胞保存技術の創生」、基礎生物学研究所IBBPセンター Cryopreservation Conference 2016.岡崎 (2016/11/10).
  • (46) Tsuda, S.:Mass preparation of fish antifreeze protein. 4th International conference on Bioprocess and Bio Therapeutics (Bioprocess2016). Houston, USA (2016/10/21).
  • (45) 津田 栄:「不凍タンパク質の構造ー氷結晶結合機能の解明とその応用ー」、2016年度日本真空学会年会.札幌 (2016年10月6日).
  • (44) 津田 栄:「不凍タンパク質とは何か?ーその構造多様性と分子機能の関係ー」、大阪大学大学院理学研究科主催講演会.大阪 (2015/11/5).
  • (43) 津田 栄:「不凍蛋白質の分子機能解明に基づく新規細胞保存技術の開発」、基礎生物学研究所IBBPセンター Cryopreservation Conference 2014.岡崎 (2014/10/24).
  • (42) Tsuda, S.:The membrane-binding ability of fish IBP prolongs the life-time of a cell dramatically. 2nd International Ice-Binding Protein Conference (IBP2014). Sapporo JPN (2014/8/7).
  • (41) 津田 栄:「不凍蛋白質の機能解明に基づく技術創生」、第1回北海道大学オープンファシリティシンポジウム.札幌 (2014/3/10).
  • (40) 津田 栄:「新技術の創生をもたらす不凍蛋白質の分子機能解明」、平成25年度北海道地区国立大学法人等技術職員研修セミナー.札幌 (2013/8/27).
  • (39)○Tsuda, S., Kondo, H., Ideta, A., Aoyagi, Y., and Nishimiya, Y. Antifreeze Protein from Japanese Organisms: Functional Analyses for the General Use. 50th Annual meeting of the society for cryobiology (CRYO2013), Washingon D.C. USA (2013/7/29).
  • (38) 津田 栄:「不凍タンパク質とは何か?ー凍結技術分野での応用可能性ー」、(株)サイエンスフォーラム主催講演会 不凍タンパク質の食品応用~新製品開発と市場拡大へのマイルストーン~.東京 (2013/2/14).
  • (37) 津田 栄:「キノコの不凍タンパク質の分子構造と不凍機能のメカニズムを解明」、第21回産総研・新技術セミナー in 弘前.弘前市 (2012/12/13).
  • (36) 津田 栄:「不凍タンパク質の機能解明に基づく産業応用展開」、H24中国地域産総研技術セミナー in 米子.米子市 (2012/12/7).
  • (35) 津田 栄:「不凍蛋白質の機能と産業応用に関する研究」、第9回メタンハイドレート産業創出イノベーション講演会.東京 (2012/11/5).
  • (34) 津田 栄:「食品分野における不凍蛋白質(AFP)の活用技術について」、日本食品工業倶楽部 東京部会(品質保証懇話会). 東京 (2012/4/24).
  • (33) 津田 栄:「不凍タンパク質の大量精製と新たな応用開拓」、平成24年度 産総研・新規採用職員初期研修(ロールモデル懇談会 講師). つくば (2012/4/11).
  • (32) Tsuda, S.:「Recent technological developments utilizing antifreeze protein」、1st International Ice-Binding Protein (IBP) Conference and Workshop, Kingston (ON), CAN (2011/8/3~6).
  • (31) Kondo, H.:「Crystal structure of antifreeze protein from a snow mold fungus」、1st International Ice-Binding Protein (IBP) Conference and Workshop, Kingston (ON), CAN (2011/8/3~6).
  • (30) 津田 栄:「不凍タンパク質の分子機能解明に基づく新技術の創成」、極限環境生物学会第12回シンポジウム. つくば (2011/6/13).
  • (29) 津田 栄:「不凍タンパク質の分子機能解析と応用技術研究」、酵素工学研究会第65回講演会. 京都 (2011/4/22).
  • (28) Tsuda, S.:「NEW TECHNOLOGIES UTILIZING ANTIFREEZE PROTEIN」、12th Int. Conference on the Physics and Chemistry of ICE (PCI-2010), Sapporo, JPN (2010/9/10).
  • (27) Sakashita, M., Nishimiya, Y., Kondo, H., and Tsuda, S.:「INTERACTION BETWEEN ICE AND A TYPE III ANTIFREEZE PROTEIN UNDER PRESSURE」、12th Int. Conference on the Physics and Chemistry of ICE (PCI-2010), Sapporo, JPN (2010/9/10).
  • (26) 津田 栄:「不凍タンパク質の分子機能解明および産業応用」、第5回メタンハイドレート研究アライアンス講演会. 札幌 (2010/9/2).
  • (25) 津田 栄:「新技術をもたらす不凍蛋白質の構造機能解析」、第10回日本蛋白質科学会. 札幌 (2010/6/16).
  • (24) 津田 栄:「新技術をもたらす不凍蛋白質の分子機能解明」、茨城県中性子利用促進研究会「生命物質構造解析研究会」. 東海 (2010/3/25).
  • (23) 西宮佳志:「氷と相互作用する不凍蛋白質?作用機序解明に向けた中性子回折実験への期待」、茨城県中性子利用促進研究会分科会・公開研究会. 東京 (2010/1/15).
  • (22) 津田 栄:「不凍タンパク質の分子機能解明と産業応用に関する研究」、室蘭工業大学・創製機能工学専攻セミナー. 室蘭 (2009/11/11).
  • (21) 津田 栄:「不凍タンパク質の分子機能解明と食品分野への応用」、第56回日本食品科学工学会大会. 名古屋 (2009/9/12).
  • (20) Tsuda, S.:「New technologies developed for practical use of antifreeze protein (Keynote Lecture)」、46th Annual Meeting of the Society for Cryobiology (CRYO2009), Sapporo, JPN (2009/7/20).
  • (19) 西宮佳志:「国産魚由来不凍タンパク質の精製と解析」、道央バイオ研究交流会. 恵庭 (2009/2/27).
  • (18) 西宮佳志:「魚から不凍タンパク質を大量に精製する」、産業技術総合研究所技術フェア. 函館 (2009/1/23).
  • (17) 津田 栄:「魚肉すり身由来不凍タンパク質による新しい凍結保存技術の開発」、産総研研究講演会in中部. 名古屋 (2007/12/5).
  • (16) 津田 栄:「魚類由来不凍タンパク質を用いた冷却エネルギー削減技術の創生」、九州大学WSフォーラム「タンパク質・ペプチド研究の現状と展望」. 福岡 (2007/11/2).
  • (15) Tsuda, S.:「Antifreeze Protein from Japanese Fish: An epoch in biotechnology」, The 234th American Chemical Society Meeting - Antifreeze Proteins: A Memorial Synposium for Robert Feeney. (*Program assigned to 'Division of Computers in Chemistry'), Boston, USA (2007/8/19).
  • (14) Tsuda, S.:「Antifreeze Protein - from functional analysis to technological applications- (Keynote Lecture)」,2nd International Symposium on the Environmental Physiology of Ectotherms and Plants (ISEPEP2) . Duniden, New Zealand (2007/7/2).
  • (13) 津田 栄:「不凍タンパク質(AFP)の謎と産業利用の可能性」、極限環境下の生物に学ぶ食品技術フォーラム. 東京 (2006/4/21).
  • (12) 津田 栄:「北の自然からの贈り物ー不凍タンパク質ー」、市民講演会 ー世界物理年にあたり、生命を新しい視点から解き明かすー. 札幌 (2005/11/26).
  • (11) 津田 栄:「純国産不凍蛋白質のバイオテクノロジー」、H16産業技術連携推進会議生命工学部会. 福岡 (2005/3/3).
  • (10) 津田 栄:「不凍タンパク質ー3次元分子構造解明から産業応用までー」、北大創成科学研究機構セミナー. 札幌 (2004/10/12).
  • (9) 矢追 克郎、近藤 英昌、鈴木 守、野呂 奈津子、津田 栄、三石 安. 「糖質加水分解酵素ファミリー74に属するキシログルカン分解酵素の機能と構造」、第12回糖質関連酵素化学シンポジウム、鹿児島 (2004/9/17).
  • (8) 津田 栄:「Antifreeze Protein - from structural analysis to industrial application -」、産総研-韓国工業科学技術会ジョイントワークショップ. 札幌 (2004/6/29).
  • (7) 津田 栄:「純国産不凍蛋白質の研究」、第30回日本低温医学会総会. 札幌 (2003/11/29).
  • (6) 津田 栄:「純国産不凍蛋白質の研究?臓器保存等への応用の可能性?」、第2回北海道海洋生物科学シンポジウム. 札幌 (2003/10/31).
  • (5) 津田 栄:「不凍蛋白質の3次元構造解析および産業応用研究」、H15産業技術連携推進会議生命工学部会. 札幌 (2003/9/18).
  • (4) 津田 栄:「不凍蛋白質?分子構造解明から産業応用まで?」、H15低温生物工学会年会. 札幌 (2003/6/26).
  • (3) 津田 栄:「純国産不凍蛋白質研究の最新状況」、第3回蛋白質科学会. 札幌 (2003/6/24).
  • (2) Tsuda, S.:「Japanese Antifreeze Proteins」, Symposium on Stress Proteins: From Antifreeze to Heat Shock. California, USA (2003/6/26).
  • (1) 津田 栄、西宮佳志:「極地魚類の低温適応 -凍結から身を守る氷晶形成阻害蛋白質-」、大阪大学蛋白質研究所セミナー. 大阪 (2003/3/11)

書籍

  • (6) 石原和成、三浦 愛、津田 栄:魚類不凍蛋白質の細胞保護機能の解析、バイオインダストリー、31 (6), 61-66 (2014).

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