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Group Introduction

Advanced Biomeasurements Research Group

Introduction to research

We are doing a wide range of studies in the field of biomeasurement (1) to develop molecular methods and bioinformatics for quantitative detection of particular nucleic acid sequences in sequence specific and/or highly multiplexed manners, (2) to develop techniques for measuring biological activities of cells, and (3) to develop techniques for quality control of high-throughput bio-measurements. We are currently studying following four topics.

1. Laboratory tool development for highly multiplexed DNA measurements

Specific nucleic acid sequence quantification is important in many molecular biological diagnostics such as viral titer determination, environmental monitoring, and the examination of genetically modified organisms. We are interested in development of methods for quantifying specific nucleic acid sequences for use in such genetic characterization, monitoring, and gene expression analysis in highly multiplexed manner. We are particularly focusing on next generation DNA sequencing (NGS) technologies and evaluating the measurement biases associated with NGS. We also have interest in developing methods for quality control of such measurements.

2. Development of molecular and bioinformatic tools for quantitative detection of microorganisms in environment

Microorganisms are an essential component of the earth’s biota, playing integral roles in ecosystems in terms of function and sustainability. In clinical aspect, part of microbes are hazardous for human and animals. To precisely monitor such organisms, the quantitative detection and identification of microbes of interest is required in clinical, pharmaceutical, and environmental microbiology. We are currently developing molecular methods to better detect and quantify particular species (or groups) of microorganisms based on DNA and RNA.

3. Development of techniques to measure biological activities

We are currently interested in overcoming several obstacles to the measurement of biological activities. First, we are studying how the viability, recovery and cell integrity of animal cells stored in cell banks should be evaluated. The quantification of cellular growth and viability has become an essential tool in any laboratory working on cell-based studies. We are currently developing rapid and more precise methods to measure the viability of cells based on direct observation of cell movements.

4. Development of microfluidics to further facilitate practical applications of bio-measurements

Microfluidics, biochip, and electrochemical sensors are one of the emerging practical application areas of bio-measurement. We are currently interested in integrating our techniques mentioned above into microfluidics devices, and biochips to further facilitate practical applications of our methods.

List of Publications

20172016201520142013201220112010

Staff:

  • Yuji Sekiguchi (Research Group Leader)

  • Penmetcha Kumar

  • Dieter Tourlousse